Current Journal of Applied Science and Technology, Mar 24, 2020
A micropropagation protocol for parthenocarpic gynoecious cucumber reduces the burden of producin... more A micropropagation protocol for parthenocarpic gynoecious cucumber reduces the burden of producing the seeds for each generation and their maintenance in-vivo. Thus an experiment was conducted in order to regenerate the plants in-vitro to check their performance after micropropagation. The micropropagation resulted in maximum shoot initiation (100%) from seedling excised cotyledonary explants with half strength MS medium supplemented with 0.5 mg/l IAA and 2 mg/l BAP along with half strength MS medium supplemented with 0.25 mg/l IAA for rooting and from stem nodal explants with Full MS + 1.5 mg/l IAA + 2 mg/l BAP media whereas half strength MS media without any hormones resulted in rooting and in both cases there were in-vitro flowers and change in their sex expression while grown in in-vivo conditions. On an average 61.11 and 48.15 percent survival was recorded from the plants regenerated through cotyledonary explants Original Research Article
Cytoplasmic male sterile system in ridge gourd has been converted to cytoplasmic genetic male ste... more Cytoplasmic male sterile system in ridge gourd has been converted to cytoplasmic genetic male sterile (CGMS) system through the development of analogues of male sterile (MS) line, maintainer line and fertility restorer line. These lines were developed by crossing the MS mutant, regenerated through in vitro culture, with monoecious pollen parents Deepthi, Haritham, LA 101, CO 2, IC 92761 and IC 92685. All hybrids and the BC 1 generation developed by crossing with the recurring pollen parents Deepthi, Haritham and LA 101 were male sterile. Male sterile BC 1 plants have been advanced to BC 6 generation and the parental line LA 101 was proved to be a successful maintainer line, producing male sterile progeny in successive back cross generations. Analogue of cytoplasmic male sterile line, MS LA 101, was developed through back crossing and on crossing with fertility restorer lines Arka Sumeet and LA 102, this line excelled as female parent, resulting heterotic combinations. Mitochondrial marker rpS14 and SCAR Tm-53 were identified to yield male sterility specific markers whereas SSR marker 18956 has generated the male fertility specific marker. These primers are recommended for marker assisted selection of ridge gourd, for utilizing male sterility for hybrid seed production and for developing A, B and C lines in CGMS system.
The genetic interrelationship among all the popular vegetable cowpea varieties cultivated in Kera... more The genetic interrelationship among all the popular vegetable cowpea varieties cultivated in Kerala was investigated through morphometric, RAPD and SSR analyses. High polymorphism was observed in RAPD (88.0 per cent of bands were polymorphic) and SSR (80.0 per cent of bands were polymorphic) analyses. Dendrogram based on morphological parameters has divided the genotypes into two main clusters which truncated at 68.0 per cent similarity, with purple and green poded varieties segregating into separate clusters. Morphometric analysis was successful to group the varieties with determinate and indeterminate growth habits whereas, RAPD and SSR markers could not make a clear division. However, distinction was apparent between purple poded and green poded types and purple poded varieties viz Vyjayanthi and Varun exhibited high similarity with each other in SSR analysis (80.0 per cent) when compared to RAPD (77.0 per cent). Dendrogram of pooled data of SSR and RAPD analyses has revealed max...
Current Journal of Applied Science and Technology, Mar 24, 2020
A micropropagation protocol for parthenocarpic gynoecious cucumber reduces the burden of producin... more A micropropagation protocol for parthenocarpic gynoecious cucumber reduces the burden of producing the seeds for each generation and their maintenance in-vivo. Thus an experiment was conducted in order to regenerate the plants in-vitro to check their performance after micropropagation. The micropropagation resulted in maximum shoot initiation (100%) from seedling excised cotyledonary explants with half strength MS medium supplemented with 0.5 mg/l IAA and 2 mg/l BAP along with half strength MS medium supplemented with 0.25 mg/l IAA for rooting and from stem nodal explants with Full MS + 1.5 mg/l IAA + 2 mg/l BAP media whereas half strength MS media without any hormones resulted in rooting and in both cases there were in-vitro flowers and change in their sex expression while grown in in-vivo conditions. On an average 61.11 and 48.15 percent survival was recorded from the plants regenerated through cotyledonary explants Original Research Article
Cytoplasmic male sterile system in ridge gourd has been converted to cytoplasmic genetic male ste... more Cytoplasmic male sterile system in ridge gourd has been converted to cytoplasmic genetic male sterile (CGMS) system through the development of analogues of male sterile (MS) line, maintainer line and fertility restorer line. These lines were developed by crossing the MS mutant, regenerated through in vitro culture, with monoecious pollen parents Deepthi, Haritham, LA 101, CO 2, IC 92761 and IC 92685. All hybrids and the BC 1 generation developed by crossing with the recurring pollen parents Deepthi, Haritham and LA 101 were male sterile. Male sterile BC 1 plants have been advanced to BC 6 generation and the parental line LA 101 was proved to be a successful maintainer line, producing male sterile progeny in successive back cross generations. Analogue of cytoplasmic male sterile line, MS LA 101, was developed through back crossing and on crossing with fertility restorer lines Arka Sumeet and LA 102, this line excelled as female parent, resulting heterotic combinations. Mitochondrial marker rpS14 and SCAR Tm-53 were identified to yield male sterility specific markers whereas SSR marker 18956 has generated the male fertility specific marker. These primers are recommended for marker assisted selection of ridge gourd, for utilizing male sterility for hybrid seed production and for developing A, B and C lines in CGMS system.
The genetic interrelationship among all the popular vegetable cowpea varieties cultivated in Kera... more The genetic interrelationship among all the popular vegetable cowpea varieties cultivated in Kerala was investigated through morphometric, RAPD and SSR analyses. High polymorphism was observed in RAPD (88.0 per cent of bands were polymorphic) and SSR (80.0 per cent of bands were polymorphic) analyses. Dendrogram based on morphological parameters has divided the genotypes into two main clusters which truncated at 68.0 per cent similarity, with purple and green poded varieties segregating into separate clusters. Morphometric analysis was successful to group the varieties with determinate and indeterminate growth habits whereas, RAPD and SSR markers could not make a clear division. However, distinction was apparent between purple poded and green poded types and purple poded varieties viz Vyjayanthi and Varun exhibited high similarity with each other in SSR analysis (80.0 per cent) when compared to RAPD (77.0 per cent). Dendrogram of pooled data of SSR and RAPD analyses has revealed max...
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Papers by Varun Roch