Papers by Vitaliy Khutoryanskiy
Chemical Bulletin of Kazakh National University
Gelatin is a proteinaceous substance composed of all the essential amino acids (except tryptophan... more Gelatin is a proteinaceous substance composed of all the essential amino acids (except tryptophan) and derived from collagen using a hydrolysis technique. Hydrogels and modified composites based on gelatin are widely used in the food industry, biomedicine, pharmaceutical industry and food packaging materials due to their biocompatibility, biodegradability, nonimmunogenicity and ability to stimulate cell adhesion and proliferation. Gelatin can absorb 5-10 times its weight of water and is the main ingredient of hard and soft capsules in pharmaceutical industry. It melts above 30°C and easily releases biologically active compounds, nutrients and drugs in human gastrointestinal tract. In addition, gelatin contains arginine-glycine-asparagine RGD-sequences in the polymer structure and contributes to various functions such as antioxidant, anti-hypertensive, anti-microbial, tissue regeneration, wound healing, enhances bone formation and anti-cancer therapy. This article reports a brief ove...
Orally administered live probiotic bacteria have gained popularity in recent years as potentially... more Orally administered live probiotic bacteria have gained popularity in recent years as potentially beneficial health supplements. However, these bacteria require additional protection if they are to survive passage through the low pH environment of the stomach after oral administration [1]. This talk will describe the use of alginates for microencapsulation of live probiotic bacteria. It will discuss the methods of microencapsulation, methods of drying alginate capsules (tray drying, freeze drying, fluidized bed drying), coating approaches (formation of single layer or multilayer coats; materials that can be used to coat alginates), as well as in vitro experimentation to demonstrate the ability of these matrices to protect live bacterial cells in gastric juice and target release to the intestine [2-4]. The efficiency of coating was evaluated using confocal microscopy with fluorescently-labeled chitosan. The mechanism of live bacteria protection from stomach acid was established using...
Thermoresponsive polymers featuring the appropriate combination of structural characteristics, i.... more Thermoresponsive polymers featuring the appropriate combination of structural characteristics, i.e. architecture, composition, and molar mass (MM), can form physically crosslinked networks in a solvent upon changes in temperature. This fascinating class of polymers finds utility in various sectors such as formulation science and tissue engineering. Here, we report a novel thermoresponsive triblock terpolymer which out-performs the most commonly used and commercially available thermoresponsive polymer, Poloxamer P407 (also known as Pluronic® F127) in terms of gelation concentration. Specifically, the in-house synthesised polymer forms gels at lower concentrations that is an advantage in biomedical applications. To elucidate the differences in their macroscale gelling behaviour, we investigate their micellization via differential scanning calorimetry, and their nanoscale self-assembly behaviour in detail by means of small-angle neutron scattering by simultaneously recording their rheo...
Chemical Bulletin of Kazakh National University, 2021
Gold nanoparticles (GNPs) protected by poly(2-ethyl-2-oxazoline) (POZ) of different molecular wei... more Gold nanoparticles (GNPs) protected by poly(2-ethyl-2-oxazoline) (POZ) of different molecular weights (Mw = 5, 50, 200 and 500 kDa) were synthesised and characterised by dynamic light scattering, nanoparticle tracking analysis, zeta potential measurement and transmission electron microscopy. It was established that the use of POZ with 50 kDa resulted in formation of GNPs with low polydispersity while POZ with greater molecular weights led to formation of more polydisperse GNPs. Fluorescent labelling of these nanoparticles was achieved through their reaction with polyethyleneglycol dithiol (8-12 kDa) as a linker molecule with subsequent reaction with 6-(iodoacetamido) fluorescein. The fluorescent nature of obtained GNPs was confirmed by the appearance of the fluorescence peak at 510 nm that is typical for fluorescein molecules and glowing of the aqueous solution under the UV irradiaton. The fluorescently-labelled GNPs are promising tool in biomedical application to monitor the biolog...
Pharmaceutics, 2021
Background: We developed a novel polymer insect repellent conjugate for extended release and decr... more Background: We developed a novel polymer insect repellent conjugate for extended release and decreased skin permeation of the volatile insect repellent p-menthane-3,8-diol (PMD). Methods: PMD was conjugated with acryloyl chloride via an ester bond to form acryloyl–PMD, which was subsequently copolymerised with acrylic acid at varying molar ratios. Copolymer structures were characterised by 1H NMR and FT-IR, analysed by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), molecular weights and reactivity ratios determined, and repellent loading assessed. Results: Using porcine liver esterases, ~45% of the insect repellent was released over five days. Penetration and permeation studies of the copolymer and free repellent using excised, full-thickness porcine ear skin showed no detectable permeation of the copolymer through skin compared to the PMD. Moreover, tape stripping revealed that over 90% of the copolymer remained on the outer surface of the skin, whereas ...
Polymers for Advanced Technologies, 2021
Gellan gum (GG) has been used to prepare polymeric carriers with prolonged retention on the eye s... more Gellan gum (GG) has been used to prepare polymeric carriers with prolonged retention on the eye surface for topical ocular drug delivery. In this work, GG was chemically modified with short poly(2‐ethyl‐2‐oxazoline) (PEtOx) chains that were expected to have minimal adhesion to mucosal tissues (mucoadhesion). The choice of synthetic procedure, solvents, and reagents has been dictated by biocompatibility of the materials and possible application in drug delivery. The grafts were synthesized via cationic ring‐opening polymerization and their living chains were attached onto deprotonated gellan backbone. The derivatives with three degrees of grafting were prepared by varying the in‐feed mass ratio of PEtOx grafts over GG. NMR and FT‐IR spectroscopies, thermogravimetric analysis, and SEC evidenced that the grafting had actually taken place. However, a greater diffusion coefficient determined for the copolymer, using diffusion‐ordered spectroscopy (NMR), in relation to the diffusion of th...
Pharmaceutics, 2020
Transmucosal drug delivery includes the administration of drugs via various mucous membranes, suc... more Transmucosal drug delivery includes the administration of drugs via various mucous membranes, such as gastrointestinal, nasal, ocular, and vaginal mucosa. The use of nanoparticles in transmucosal drug delivery has several advantages, including the protection of drugs against the harsh environment of the mucosal lumens and surfaces, increased drug residence time, and enhanced drug absorption. Due to their relatively simple synthetic methods for preparation, safety profile, and possibilities of surface functionalisation, silica nanoparticles are highly promising for transmucosal drug delivery. This review provides a description of silica nanoparticles and outlines the preparation methods for various core and surface-functionalised silica nanoparticles. The relationship between the functionalities of silica nanoparticles and their interactions with various mucous membranes are critically analysed. Applications of silica nanoparticles in transmucosal drug delivery are also discussed.
Chemical Bulletin of Kazakh National University, 2020
Chitosan is a natural biopolymer, polysaccharide, a product of chitin deacetylation. Chitosan is ... more Chitosan is a natural biopolymer, polysaccharide, a product of chitin deacetylation. Chitosan is a non-toxic, biocompatible and biodegradable polymer with high biological activity and stability in the environment. In addition, chitosan is obtained from natural renewable resources and is an inexpensive substance. Due to all these properties, chitosan is widely used in practical medicine, for example, in the form of hydrogel dosage forms in combination with natural and synthetic polymers. This review is focused on polymer hydrogel materials based on chitosan. Special attention is paid to the preparation and use of wound dressings for the treatment of wounds of various etiologies. The use of hydrogel wound dressings based on this polysaccharide allows to create a protective shell on the surface of various wounds, to prolong delivery of antibacterial agents, peptides and other active substances, which significantly increases the effectiveness of therapy. Bactericidal and sorption proper...
Chemical Bulletin of Kazakh National University, 2020
Some recommendations presented on the preparation of chemistry papers for publication in peer-rev... more Some recommendations presented on the preparation of chemistry papers for publication in peer-reviewed journals, indexed in Scopus and Web of Science. Some advice given on the selection of a journal for publication. Impact factors and other types of classifications for different chemistry journals and publication business models by publishers briefly discussed. Some publishers specialising on publication of chemistry papers considered. Recommendations given on the preparation of each section in a manuscript, quality of illustrations as well as the documents necessary for submission of these to the journal. Some approaches used to revise the manuscript and to deal with reviewer comments also briefly discussed. This work is prepared based on the course “How to write a paper” delivered by the author at the University of Reading (United Kingdom) for a number of years for post-graduate students from physical natural sciences as well as the courses delivered in some institutions of China,...
Journal of Chemical Technology & Biotechnology, 2017
Food Hydrocolloids, 2018
A novel concept is proposed in which alginate capsules containing a model probiotic Lactobacillus... more A novel concept is proposed in which alginate capsules containing a model probiotic Lactobacillus plantarum strain are coated with different surfactants with the aim to enhance cell survival during passage initially through simulated gastric (SGF) and then intestinal (SIF) fluid. The surfactants investigated included the anionic sodium dodecyl sulphate (SDS) and ammonium lauryl sulphate (ALS), the cationic dimethyldioctadecylammonium chloride (DDAC), benzalkonium chloride (BZK) and hexadecyltrimethylammonium bromide (CTAB), and the zwitterionic lecithin. Coating the alginate capsules with CTAB, BZK, ALS and SDS resulted in worst survival (~ 4-9 log CFU/g decrease) compared to uncoated capsules (~3 log CFU/g decrease), after 1 hour exposure to SGF and two hours in SIF, which was most likely associated with their gradual penetration inside the microcapsules, as shown by confocal microscopy, and their antimicrobial effects. Coating the alginate capsules with DDAC improved cell survival compared to uncoated capsules (~1.2 CFU/g decrease), whereas coating with lecithin improved cell survival considerably, resulting in almost complete recovery of viable cells in SGF and SIF (~ 0.3 log CFU/g decrease). Although the interaction between alginate and lecithin was relatively weak as demonstrated by turbidity and contact angle measurements, it is likely that the protection was associated with the fact that lecithin was able to penetrate into the capsule rapidly, an observation that was supported by the fact that lecithin enhanced the viability of free cells in SGF and SIF. Lecithin has significant potential of being used as a coating material for probiotic containing capsules.
Polymers, 2018
Poly(2-ethyl-2-oxazoline) has become an excellent alternative to the use of poly(ethylene glycol)... more Poly(2-ethyl-2-oxazoline) has become an excellent alternative to the use of poly(ethylene glycol) in pharmaceutical formulations due to its valuable physicochemical and biological properties. This work presents a formulation of poorly-water soluble drug, hydrocortisone, using interpolymer complexes and physical blends of poly(2-ethyl-2-oxazoline)s and two Carbopols ® (Carbopol 974 and Carbopol 971) for oromucosal administration. The swelling, hydrocortisone release and mucoadhesive properties of a series of tablet formulations obtained by combination of different Carbopols with poly(2-ethyl-2-oxazoline)s of different molecular weights have been evaluated in vitro.
Eurasian Chemico-Technological Journal, 2017
The complexation of linear chitosan with hydrogel of poly(acrylic acid) was studied in acetic aci... more The complexation of linear chitosan with hydrogel of poly(acrylic acid) was studied in acetic acid solutions. It was found that the complexation is accompanied by contraction of hydrogel samples with formation of turbid layer on their surface. The dynamic changes of swelling ratio of poly(acrylic acid) hydrogel in course of the interaction with chitosan are interpreted from the diffusion theory point of view considering the properties of double electric layer on hydrogel-solution boundary. The FTIR spectroscopy method revealed the electrostatic mechanism of interaction between poly(acrylic acid) hydrogel and chitosan. The spectrum of polycomplex shows the bands, which are characteristic for both poly(acrylic acid) (1725, 1450, 1249 cm-1) and chitosan (1648, 1536, 1165, 1091, 1023 cm-1) confirming the sorption of the later polymer by hydrogel of poly(acrylic acid). The possibility of encapsulation of antibiotic levomycetin into polycomplex hydrogels as well as its release from the capsules has been studied. It was found that the maximal value of the drug released from the polycomplex capsule is achieved after the longer period in comparison with control experiment with its release from pure PAA hydrogel. It was shown that the interpolymer interactions between oppositely charged linear polymers and hydrogels could be successfully used for preparation of encapsulated forms of various physiologically active substances.
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Eurasian Chemico-Technological Journal, 2017
Complex formation between bovine serum albumin and water-soluble synthetic cationic polyelectroly... more Complex formation between bovine serum albumin and water-soluble synthetic cationic polyelectrolyte poly[2-methacryloyloxy)ethyl]trimethyl ammonium chloride has been studied in aqueous solutions by turbidimetric and viscometric methods. It was found that the structure of polycomplex is compact and its stability strongly depends on the environment. Formation of insoluble polycomplexes is observed in solutions with low ionic strength and pH, higher than 5.0. This pH value corresponds to the isoelectric point of the protein, so at lower pH the biopolymer macromolecules gain the positive charge and not able to be bound by the positively charged macromolecules of poly[2-methacryloyloxy)ethyl]trimethyl ammonium chloride. An increase of pH within 5.0-11.0 leads to further stabilization of polycomplex because of appearance of additional negative charges on biomacromolecules, caused by ionization of acidic groups. It was found that the main forces, which are responsible for the complexation,...
International Journal of Pharmaceutics, 2017
Chemotherapeutic agents administered intravesically to treat bladder cancer have limited efficacy... more Chemotherapeutic agents administered intravesically to treat bladder cancer have limited efficacy due to periodic dilution and wash-out during urine formation and elimination. This review describes the pathophysiology, prevalence and staging of bladder cancer, and discusses several formulation strategies used to improve drug residence within the bladder. These include the use of amphiphilic copolymers, mucoadhesive formulations, hydrogels, floating systems, and liposomes. Various in vitro and in vivo models recently employed for intravesical drug delivery studies are discussed. Some of the challenges that have prevented the clinical use of some promising formulations are identified.
Eurasian Chemico-Technological Journal, 2016
The formation of H-bonded interpolymer complexes between linear and cross-linked poly(acrylic aci... more The formation of H-bonded interpolymer complexes between linear and cross-linked poly(acrylic acid) and poly(vinyl ether of ethyleneglycol) is considered. The effects of pH and concentrations of polymers solution as well as the nature of the solvent on the complexation processes are discussed. It was found that the complexes are formed in aqueous solutions only below some critical pH value, which depends on the concentration of polymers and ionic strength of the solution. The interaction between gel of poly(acrylic acid) swollen in alcohol with solution of linear poly(vinyl ether of ethyleneglycol) is accompanied by formation of turbid layer of polycomplex and contraction of gel.
Chemical Bulletin of Kazakh National University, 2015
Journal of Chemical Technology & Biotechnology, 2015
BACKGROUND: Chemical chitin extraction generates large amounts of wastes and increases partial de... more BACKGROUND: Chemical chitin extraction generates large amounts of wastes and increases partial deacetylation of the product. Therefore, the use of biological methods for chitin extraction is an interesting alternative. The effects of process conditions on enzyme assisted extraction of chitin from the shrimp shells in a systematic way were the focal points of this study. RESULTS: Demineralisation conditions of 25C, 20 min, shells-lactic acid ratio of 1:1.1 w/w; and shellsacetic acid ratio of 1:1.2 w/w, the maximum demineralisation values were 98.64 and 97.57% for lactic and acetic acids, respectively. A total protein removal efficiency of 91.10% by protease from Streptomyces griseus with enzyme-substrate ratio 55 U/g, pH 7.0 and incubation time 3 h i s o b t a i n e d w h e n t h e particle size range is 50-25 μm, which was identified as the most critical factor. The X-ray diffraction and 13 C NMR spectroscopy analysis showed that the lower percent crystallinity and higher degree of acetylation of chitin from enzyme assisted extraction may exhibit better solubility properties and less depolymerisation in comparison with chitin from the chemical extraction. CONCLUSION: The present work investigates the effects of individual factors on process yields, and it has shown that, if the particle size is properly controlled a reaction time of 3 h is more than enough for deproteination by protease. Physicochemical analysis indicated that the enzyme assisted production of chitin seems appropriate to extract chitin, possibly retaining its native structure.
Chemical Bulletin of Kazakh National University, 2012
Limonium gmelinii өсімдік түрінің химиялық зерттеулері, оның негізінде кең ауқымды əсер беруші эф... more Limonium gmelinii өсімдік түрінің химиялық зерттеулері, оның негізінде кең ауқымды əсер беруші эффективті препарат алу жəне практикалы медицинаға ұсыну мəліметтері берілген.
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Papers by Vitaliy Khutoryanskiy