Papers by Thiago Sidney Valentim Pereira
Eletrônica de Potência, 2020
Frontiers in immunology, 2018
In chronic schistosomiasis, liver fibrosis is linked to portal hypertension, which is a condition... more In chronic schistosomiasis, liver fibrosis is linked to portal hypertension, which is a condition associated with high mortality and morbidity. High mobility group box 1 (HMGB1) was originally described as a nuclear protein that functions as a structural co-factor in transcriptional regulation. However, HMGB1 can also be secreted into the extracellular milieu under appropriate signal stimulation. Extracellular HMGB1 acts as a multifunctional cytokine that contributes to infection, injury, inflammation, and immune responses by binding to specific cell-surface receptors. HMGB1 is involved in fibrotic diseases. From a clinical perspective, HMGB1 inhibition may represent a promising therapeutic approach for treating tissue fibrosis. In this study, we demonstrate elevated levels of HMGB1 in the sera in experimental mice or in patients with schistosomiasis. Using immunohistochemistry, we demonstrated that HMGB1 trafficking in the hepatocytes of mice suffering from acute schistosomiasis wa...
PLOS Neglected Tropical Diseases, 2016
Revista Ilha Digital, Jul 23, 2012
Digestive diseases and sciences, Jan 24, 2015
Nonalcoholic fatty liver disease (NAFLD) is the number one cause of chronic liver disease and sec... more Nonalcoholic fatty liver disease (NAFLD) is the number one cause of chronic liver disease and second indication for liver transplantation in the Western world. Effective therapy is still not available. Previously we showed a critical role for caspase-2 in the pathogenesis of nonalcoholic steatohepatitis (NASH), the potentially progressive form of NAFLD. An imbalance between free coenzyme A (CoA) and acyl-CoA ratio is known to induce caspase-2 activation. We aimed to evaluate CoA metabolism and the effects of supplementation with CoA precursors, pantothenate and cysteine, in mouse models of NASH. CoA metabolism was evaluated in methionine-choline deficient (MCD) and Western diet mouse models of NASH. MCD diet-fed mice were treated with pantothenate and N-acetylcysteine or placebo to determine effects on NASH. Liver free CoA content was reduced, pantothenate kinase (PANK), the rate-limiting enzyme in the CoA biosynthesis pathway, was down-regulated, and CoA degrading enzymes were incr...
Journal of Cosmology and Astroparticle Physics, 2015
Clinical science (London, England : 1979), Jan 21, 2015
Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibr... more Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibrosis that develops during chronic infection. The mechanisms whereby the pathogen evokes these host responses remain unclear. We evaluated the hypothesis that schistosome eggs release factors that directly stimulate liver cells to produce Osteopontin (OPN), a profibrogenic protein that stimulates hepatic stellate cells to become myofibroblasts. We also investigated OPN's utility as a biomarker of fibrosis and/or portal hypertension severity. Cultured cholangiocytes, kupffer cells and hepatic stellate cells were treated with soluble egg antigen (SEA); OPN production was quantified by qRTPCR and ELISA; cell proliferation assessed by BrdU. Mice were infected with S. mansoni for 6 or 16 weeks to cause early or advanced fibrosis. Liver OPN was evaluated by qRTPCR and immunohistochemistry, and correlated with liver fibrosis and serum OPN. Livers from patients with schistosomiasis mansoni (e...
Brazilian Journal of Physics, 2015
Spectroscopy: An International Journal, 2012
The aim of the present study is to evaluate the differences on FTIR spectra of the normal lung ce... more The aim of the present study is to evaluate the differences on FTIR spectra of the normal lung cell (noncancerous mice lung epithelial cell line e10) due to different fixation protocols for histological processing. The results shown that formalin and methacarn (normally used in fixation) did cause many changes on the FTIR spectra of mice lung cells e10, mainly in the organic compounds (800–1800 cm−1) in lipids, DNA, and proteins, and the alcohol 70% fixation protocol caused almost no changes on the FTIR spectra compared to unfixed cells spectra (in PBS). It can be concluded that histological processing with alcohol 70% fixation protocol can be used in the FTIR study of mice lung cell line e10.
Journal of Cosmology and Astroparticle Physics, 2014
Journal of Ovarian Research, 2014
Monthly Notices of the Royal Astronomical Society, 2013
Liver International, 2009
Liver International, 2012
Laboratory Investigation, 2010
Journal of Cosmology and Astroparticle Physics, 2009
Journal of Cosmology and Astroparticle Physics, 2012
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Papers by Thiago Sidney Valentim Pereira