Papers by Teresita Borromeo
Journal of Tropical Agriculture and Food Science, 2013
Diversity among 80 Malaysian rice accessions was assessed based on 119 Simple Sequence Repeat (SS... more Diversity among 80 Malaysian rice accessions was assessed based on 119 Simple Sequence Repeat (SSR) markers. The Polymorphic Information Content (PIC) values of 89% of the SSR markers were above 0.50 indicating that those markers were highly informative. The presence of Quantitative Trait Loci (QTLs) was assessed based on 45 markers linked to drought grain yield QTLs. Five drought grain yield QTLs were present in the 80 Malaysian rice accessions. The highest frequency was 10.6% found in qDTY12.1 present in Way Rarem, Boewani, Pulut Malaysia 1, CI–9534, IR1561–243–5–6, CICA4, IR2797–156–3, Chianung Sen Yu, MR 142 and Huma Wangi Lenggong. Second highest was qDTY3.1 present in 8.5% of the germplasm, observed in Apo, Mokwoo, Way Rarem, CICA 4, Biris, MR 185, Tainan and Q70. Two UPGMA cluster analysis were performed based on 119 SSR markers and 45 specific SSR markers which linked to QTLs for grain yield under drought. The rice accessions were grouped at 77% similarity coefficient and produced 7 clusters using 119 SSR markers and 9 clusters using 45 specific SSR markers. Keywords: SSR markers; PIC; similarity coefficient; cluster analysis
Journal of Tropical Agriculture and Food Science, 2012
The rice accessions reached the 50% flowering stage at the end of March, indicating that the crop... more The rice accessions reached the 50% flowering stage at the end of March, indicating that the crops underwent severe stress for about 2 weeks during panicle development and pre-flowering stages. All traits were drastically affected by the drought stress and significant reduction in their performance was also observed. Analysis of variance showed significant differences for all phenotypic and agronomic traits observed under stress environment. Heritability showed four phenotypic and agronomic traits, namely, days to 50% flowering, plant height, flag leaf length and flag leaf width. These traits showed high heritability both under non-stress (control) (67.7 – 82.6%) and stress (64.1 – 89.7%) environments. Grain yield had high heritability under non-stress environment (62.5%) but moderate heritability under stress environment (50.0%). Several significant positive as well as negative correlations under stress environment were observed among the traits. Similar trends of correlation were also observed under non-stress environments. Keywords: Keywords: growth performance; rice germplasm; drought stress
Philippine Agricultural Scientist, Mar 24, 2010
The genetic diversity of 22 accessions of the genus Garcinia was assessed using peroxidase, RAPD ... more The genetic diversity of 22 accessions of the genus Garcinia was assessed using peroxidase, RAPD markers and gene sequence specific amplification polymorphism (GSSAP). Among the 15 isozymes tested, only peroxidase produced reproducible, polymorphic bands with a polymorphism information content (PIC) of 0.79. A total of eight bands were generated forming three fingerprint patterns distinct for G. mangostana, G. binucao, G. kydia and G. lateriflora. No bands were observed for G. livingstonei and G. xanthochymus. The three RAPD primers showed high PIC of 0.92 (OPB-04), 0.78 (OPB-06) and 0.91 (OPB-07). For GSSAP markers, two sets of primers based on the conserved regions of acyl-ACP thioesterase (ACYL-ACP), and chalcone synthase (CHALCS) had relative PICs of 0.75 and 0.89 for ACYL-ACP and CHALCS, respectively. The high PICs indicate the capability of these techniques to quantify genetic diversity in Garcinia species. The dendrograms using UPGMA-SAHN cluster analysis based on peroxidase, RAPD and GSS amplification polymorphism showed that Garcinia species clustered into five groups at mean similarity coefficient 0.54. Group I consisted of all 17 G. mangostana accessions and was further classified into three subgroups (Ia, Ib and Ic). Group II composed of G. kydia and G. lateriflora showed a genetic similarity of 0.94. G. livingstonei, G. xanthochymus and G. binucao were unique in their groups. This study showed that the G. mangostana accessions analyzed had low genetic variation and that the different species can be clearly distinguished by combined peroxidase, RAPD and gene sequence specific amplification polymorphism. Key Words: DNA markers, diversity analysis, Garcinia, gene-specific markers, isozymes, mangosteen, RAPD
Indian journal of agricultural research, Jun 1, 2019
Atuna racemosa Raf., a forest tree used to season raw fish dish in the Philippines, is proven to ... more Atuna racemosa Raf., a forest tree used to season raw fish dish in the Philippines, is proven to have antibacterial properties. The study aimed to help elucidate the significance of using its kernel as a condiment and consequently, increase its value by assessing its antimicrobial and phytochemical properties. The antimicrobial activity of its ethanolic extracts were screened against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica ser. Typhimurium, Aspergillus niger, Saccharomyces cerevisiae and Candida albicans using agar disc diffusion technique. Qualitative phytochemical screening was done and total phenolic content was determined at a concentration of 100 mg dry sample per 1 mL solvent. The extract showed bacterial growth inhibition in Staphylococcus aureus, Pseudomonas aeruginosa and Bacillus cereus while a mild antifungal activity against Aspergillus niger was observed. Phytochemical analysis detected the presence of cardiac glycosides, tannins, phenolic compounds and saponins in the extract. It yielded a total phenolic content of 42.7 mg GAE/100 g. This denotes that the kernel of A. racemosa has the potential to be classified as a natural food preservative and functional food.
Philippine Agricultural Scientist, 2000
Philippine Journal of Crop Science, 2001
Philippine Journal of Crop Science, 1993
한국작물학회 학술발표대회 논문집, Oct 1, 2012
Plant Systematics and Evolution, 2000
ABSTRACT To determine the taxonomic status and to clarify the biosystematic relationships of wild... more ABSTRACT To determine the taxonomic status and to clarify the biosystematic relationships of wild rice species, i.e.,Oryza granulata andO. meyeriana in theO. meyeriana complex (Poaceae), a series of intraspecific and interspecific hybridization experiments, genomic analyses through meiotic pairing, and morphological studies were conducted. Hybridization results demonstrate comparatively high crossability with about 34–39% of seed set, and very limited reproductive isolation betweenO. granulata andO. meyeriana. Chromosome pairing in the parental species and their F1 hybrids indicate very high genomic affinity between the two taxa. Morphological analyses of the parental accessions and their intra- and interspecific hybrids show a large variation among the samples included, but no appreciable grouping was observed. The traditionally used character, i.e., spikelet length, does not contribute significantly to the grouping of the samples included. Based on the facts that the two taxa have negligible reproductive barriers and share almost identical genomes, the authors believe thatO. granulata andO. meyeriana do not warrant separate taxonomic status, and recommend that they be combined asOryza granulata.
Journal of bioscience and agriculture research, 2016
The study was conducted at International Rice Research Institute (IRRI) to assess the response of... more The study was conducted at International Rice Research Institute (IRRI) to assess the response of dry season hybrid seeds including parental checks FL478 and NSIC Rc222 to salt tolerance (12 dS/m) at the seedling stage of rice using IRRI screening techniques. The total number of seeds were two hundred thirty-one and irrigated, flood prone, heat tolerance, salinity and problem soils, aerobic and anaerobic germination, rainfed lowland and South Asian samples were used for this experiment. Among them, only 1.73% populations (4 irrigated) were identified as tolerant, 18.18% moderately tolerant, 37.26% sensitive and 46.86% were highly sensitive. Moderately tolerant plants were found from irrigated, flood prone, salinity and problem soils, aerobic germination, anaerobic germination and rainfed lowland and South Asian genotypes. As there was Brown Plant Hopper (BPH) infestation in this experiment, the tolerant genotypes may also be tolerant to BPH and for that reason results were distorted for other genotypes. Therefore, study should be conducted under controlled environment to ascertain the level of salt tolerance of the moderately tolerant populations. Besides, the identified tolerant genotypes (4) should be further tested with 18 dS/m to determine their supremacy to salt tolerance at the seedling and reproductive stages and QTL analysis could be performed to determine the effects of each genomic region of the trait of interest.
Plant Genetic Resources, Jun 29, 2023
V. bicolor, V. trifolia s. str. and V. rotundifolia are part of a species complex that has record... more V. bicolor, V. trifolia s. str. and V. rotundifolia are part of a species complex that has recorded medicinal use in the Philippines. We assembled the first chloroplast genome of V. bicolor through next-generation sequencing and compared this to earlier established chloroplast genomes of V. trifolia s. str. and V. rotundifolia to provide additional insights into their genotypic differences. To ensure the continued utility of the research outputs in case of future taxonomic revisions, we characterized the morphology of PBN 2018-674, the reference germplasm utilized to generate the plastome. The complete chloroplast genome sequence of V. bicolor was 154,460 bp long with 131 coding genes comprising 87 mRNA genes, 36 tRNA genes and 8 rRNA genes. Using a separate accession from a different type locality, an identical chloroplast genome was equally established, indicating its conserved nature. When compared to V. trifolia s. str. and V. rotundifolia, slight variations were observed in gen...
Journal of Forest Research, Feb 27, 2023
Mitochondrial DNA Part B, Nov 2, 2022
Philippine Journal of Crop Science, 2001
... ANALEN MDELA ROSA1,TOMAS MMASAJO2,SENEN HESCAMOS1,ERNESTO BCAYABAN JR1, DANILO JLALICAN1,TERE... more ... ANALEN MDELA ROSA1,TOMAS MMASAJO2,SENEN HESCAMOS1,ERNESTO BCAYABAN JR1, DANILO JLALICAN1,TERESITA HBORROMEO1 &JOSE EHERNANDEZ1 1 ... On Hybrid Rice, India Virmani, SS, BC Viraktamath, CL Casal, RS Toledo, MT Lopez & JO Manalo. ...
International Journal of Disaster Resilience in The Built Environment, Aug 29, 2019
Purpose-Sarangani, a province in Southern Philippines, is inhabited predominantly by tribal group... more Purpose-Sarangani, a province in Southern Philippines, is inhabited predominantly by tribal groups who depend on traditional rice farming for subsistence and livelihood. The purpose of this study is to identify current pressures to these upland communities and the interventions instituted to address them or mitigate their effects. Design/methodology/approach-This is an exploratory and cross-sectional research using the emic approach. Rapid rural appraisal techniques (i.e. focus group discussion, key informant interviews, community immersion and field observation) were concurrently undertaken in 15 farming villages in the Sarangani uplands. Findings-Results revealed that many upland families inhabited disaster-prone areas under conditions of hardship and abject poverty. Prevalent problems in these areas have largely arisen from the encroachment of modern agriculture, environmental degradation and changes in the socio-political and economic spheres. Consequently, food insecurity, cultural and genetic erosion and biodiversity losses have resulted in lowered Sarangani agro-ecosystem resilience. While policies and programs had been instituted to address these problems, positive results still remain to be realized.
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Papers by Teresita Borromeo