Papers by Taukhid Taukhid
E3S Web of Conferences, 2021
Disease outbreaks and mortality in giant gourami and Asian catfish (pangasius) culture have been ... more Disease outbreaks and mortality in giant gourami and Asian catfish (pangasius) culture have been frequently reported during the last decade. Etiological agents of the disease have remained unknown, so it is needed to explore epidemiological studies to know the main causative agents. The study aimed to know the main pathogenic agents who were identified during disease outbreaks on giant gourami and pangasius. Data and information were collected through active and passive surveillance at the production centers of each species. Analysis was carried out according to standard fish disease diagnosis and eventually was focused on two suspected new emerging diseases (NED), namely Infectious Spleen and Kidney Necrosis Virus (ISKNV) belonging to Megalocytivirus on giant gourami and Enteric Septicemia of Catfish (ESC) caused by Edwardsiella ictaluri on pangasius. The results showed that ISKNV was detected on most of the diseased giant gourami samples and that E. ictaluri was identified on most...
E3S Web of Conferences, 2021
Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing los... more Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing losses estimated up to IDR 15.0 billion annually. This study aims to assess the efficacy of bivalent and trivalent vaccines containing Streptococcus agalactiae bacteria on tilapia. The formula of the bivalent vaccine contains 75% of S01-196-16 and 25% of N14G isolates (v/v). Trivalent vaccine contains 30%, 35%, and 35% of N14G, NP1050, and SG01-16 isolates (v/v), respectively. A challenge test assessed the efficacy of the vaccines, and it was carried out at 30, 90, and 150 days post-vaccination by artificially infection at LD60. Selected bacteria isolate to be appointed in the challenge test are N14G (biotype 2) and S01-196-16 (biotype 1). Relative Percentage of Survival (RPS) was used as the main indicator of vaccine efficacy. The results revealed that the highest RPS of a bivalent vaccine against S. agalactiae (S01-196-16) was achieved at the first challenge (61.84%), and trivalent vaccin...
E3S web of conferences, 2021
The disease is the main agent that causes mortality of fish, especially during seed stages. The r... more The disease is the main agent that causes mortality of fish, especially during seed stages. The research aimed to find out bacteria and parasitic speciesin glass eel, Anguilla spp. Bacterial identification was carried out by a biochemical method. The prevalence of bacterial species was calculated using the El-Gohary et al. (2020) formula, while the results of bacterial identification from glass eel were Aeromonas spp., Vibrio spp., Enterococcus spp., Staphylococcus spp., Planococcus spp., Lactobacillus spp., Listeria spp., Citrbacterfreundii, Neisseria spp., Pseudomonas aeruginosa, Kurthia spp., Streptococcus spp., and Corynebacterium spp. It was found that the five highest prevalence rate was for Listeria spp. (39.64%), followed by Aeromonas spp. (26.13%), Staphylococcus spp. (16.22%), Corynebacterium spp. (5.41%), Lactobacillus spp. (2.70%), and the lowest prevalence rate was Streptococcus spp. (0.90%). The type of parasitic pathogen obtained was Trichodina spp. (2,70%), Dactylogyrus spp. (2,70%) and Gyrodactylus spp. (2,70%). Bacterial and parasites identified in glass eels need further verification on the epizootiology characteristic of each pathogenic agent.
E3S web of conferences, 2021
Alternative prevention should be developed to control disease outbreaks in Tilapia culture caused... more Alternative prevention should be developed to control disease outbreaks in Tilapia culture caused by co-infections of Aeromonas hydrophila and Streptococcus agalactiae. Applications of vaccines with low cost and simple methods are highly recommended. This study was conducted to find out the safety and the efficacy of immersion vaccine in Tilapia used bivalent vaccines with formalin inactivated of S. agalactiae and A. hydrophila. Tilapia (Oreochromis niloticus) were divided into two groups, vaccinated and not vaccinated, with single and double doses immersion. The results showed that single and double doses of immersion vaccine were safe for fish (SR 100%) and effectively increased the antibody titer values compared to non-vaccinated fish. The immersion vaccine method can be recommended as an alternative to controlled fish diseases, especially for both Motile Aeromonads Septicaemia and Streptococcosis caused by A. hydrophila and S. agalactiae infections, respectively.
Bulletin of The European Association of Fish Pathologists, 2017
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License Dry green leaves of Indian almond (Terminalia catappa) to prevent streptococcal infection in juveniles of the Nile tilapia (Oreochromis niloticus)
Indonesian Aquaculture Journal, 2020
Co-infections occur when hosts are infected by two or more pathogens either simultaneous or as a ... more Co-infections occur when hosts are infected by two or more pathogens either simultaneous or as a secondary infection. The aim of this research was to know the best vaccine composition regarding a combined vaccine and the efficacy of the best vaccine dose for protection against co-infections. In treatment 1: Vaccination was done by immersion with a dosage of 10 mL KHV and Aeromonas hydrophila Liter -1 . One liter was used for immersion of 10 carp with a size between 5-7cm. The vaccine treatment was consisted of four main factors, and for each treatment 200 fish in four groups with a repetition was used of 4 times. For treatment 2: The best proportional combination from treatment 1, then will be tested on koi by immersion and fish is kept in aquaria 60 cm x 70cm x 40cm and filled with water as much as 30 liters per container. Each treatment was proceeded with 200 fish in four groups with 4 replications. Fish are fed with commercial pellets twice a day every morning and evening. In con...
Efficacious of in-active bacterial vaccines: Aeromonas hydrophila-AHL0905-2 (Hydrovac) and Strept... more Efficacious of in-active bacterial vaccines: Aeromonas hydrophila-AHL0905-2 (Hydrovac) and Streptococcus agalactiae-N14G (Streptovac) for prevention of bacterial diseases on fresh water aquaculture. By: Taukhid, Uni Purwaningsih, Desy Sugiani, Tuti Sumiati, and Angela Mariana Lusiastuti The study with aimed to determine the performance of hydrovac and streptovac vaccines for prevention of bacterial disease, namely motile aeromonad septicemia (MAS) and streptococcosis diseases on freshwater aquaculture has been carried out at laboratory and field scales. The fish used in the study were catfish, tilapia and giant gouramy. The vaccine was administered by immersion technique at the dose and period of immersion refers to standard instructions of product label. Vaccine effectiveness was evaluated based on survival rate, and then calculated the relative percentage survival (RPS). The study results revealed that the RPS value of hydrovac vaccine on the laboratory scale was ranged between 52...
HAYATI Journal of Biosciences, 2022
In Indonesia, the Nile tilapia (Oreochromis niloticus) is the most widely farmed and available fi... more In Indonesia, the Nile tilapia (Oreochromis niloticus) is the most widely farmed and available fish for consumption. Production loss due to bacterial infection by Aeromonas hydrophila and Streptococcus agalactiae is the main problem in tilapia cultivation. This study aimed to determine the occurrence of concurrent infection of Aeromonas hydrophila AHL 0905-2 and Streptococcus agalactiae N14G in Nile tilapia based on biochemical and molecular characteristics. From the results of biochemical assay and sequence analysis of the 16S rRNA fragment, Aeromonas hydrophila and Streptococcus agalactiae were confirmed. Genes for aerolysin (417 bp), nuclease (504 bp), lipase (155 bp), and serine protease (211 bp) were found in Aeromonas hydrophila AHL 0905-2, while Streptococcus agalactiae N14G was determined as a 1b serotype group that had genes for CPS L (688 bp), CPSG (621 bp), and CPS J (272 bp). The confirmation in tilapia of Aeromonas hydrophila and Streptococcus agalactiae by PCR and sequ...
E3S Web of Conferences, 2021
The disease is the main agent that causes mortality of fish, especially during seed stages. The r... more The disease is the main agent that causes mortality of fish, especially during seed stages. The research aimed to find out bacteria and parasitic speciesin glass eel, Anguilla spp. Bacterial identification was carried out by a biochemical method. The prevalence of bacterial species was calculated using the El-Gohary et al. (2020) formula, while the results of bacterial identification from glass eel were Aeromonas spp., Vibrio spp., Enterococcus spp., Staphylococcus spp., Planococcus spp., Lactobacillus spp., Listeria spp., Citrbacterfreundii, Neisseria spp., Pseudomonas aeruginosa, Kurthia spp., Streptococcus spp., and Corynebacterium spp. It was found that the five highest prevalence rate was for Listeria spp. (39.64%), followed by Aeromonas spp. (26.13%), Staphylococcus spp. (16.22%), Corynebacterium spp. (5.41%), Lactobacillus spp. (2.70%), and the lowest prevalence rate was Streptococcus spp. (0.90%). The type of parasitic pathogen obtained was Trichodina spp. (2,70%), Dactylogy...
E3S Web of Conferences, 2021
Alternative prevention should be developed to control disease outbreaks in Tilapia culture caused... more Alternative prevention should be developed to control disease outbreaks in Tilapia culture caused by co-infections of Aeromonas hydrophila and Streptococcus agalactiae. Applications of vaccines with low cost and simple methods are highly recommended. This study was conducted to find out the safety and the efficacy of immersion vaccine in Tilapia used bivalent vaccines with formalin inactivated of S. agalactiae and A. hydrophila. Tilapia (Oreochromis niloticus) were divided into two groups, vaccinated and not vaccinated, with single and double doses immersion. The results showed that single and double doses of immersion vaccine were safe for fish (SR 100%) and effectively increased the antibody titer values compared to non-vaccinated fish. The immersion vaccine method can be recommended as an alternative to controlled fish diseases, especially for both Motile Aeromonads Septicaemia and Streptococcosis caused by A. hydrophila and S. agalactiae infections, respectively.
Indonesian Aquaculture Journal, 2008
Penaeid shrimp culture is a major contributor to foreign exchange earning in Indonesia. It has si... more Penaeid shrimp culture is a major contributor to foreign exchange earning in Indonesia. It has significant impact on economic development of fisheries sector, and leads to be one of prime mover to improve social prosperity. However, shrimp industry particularly black tiger shrimp (Penaeus monodon) has been facing unpredictable situation due to disease problem. The main constrain in correlation to the development of shrimp industry is disease outbreak, especially caused by viral agents. White Spot Syndrome Virus (WSSV) occurred in 1994, causing mass mortality of black tiger shrimp almost in all of the middle and western part of Indonesia. Due to the disease problem, it is estimated that in year 2000, more than 50% of shrimp pond were idle. Pacific white shrimp (Litopenaeus vannamei) or “udang vanamei” was introduced to Indonesia at the end of 1999, and released officially in July, 2001. Response of shrimp farmers to the shrimp rapidly accepted and distributed to many provinces in the...
Indonesian Aquaculture Journal, Jan 10, 2021
Indonesian Aquaculture Journal, 2008
The aquaculture industry in Indonesia has been growing rapidly and plays an important role in rur... more The aquaculture industry in Indonesia has been growing rapidly and plays an important role in rural development and export earning. Penaeid shrimp culture in Indonesia has become a leading export earning in fisheries sector. The main constraint encountered with shrimp culture has always been associated with disease outbreaks, especially, caused by viral agents. The Pacific white shrimp (Litopenaeus vannamei) was unofficially introduced to Indonesia in 1999, and officially approved by Indonesian government in 2001. By the end of 2007, the Pacific white shrimp has been cultured in more than 17 provinces. The Taura Syndrome (TS) disease was detected in Indonesia in 2002, and the disease is currently found in at least 10 provinces. The Infectious Myonecrosis (IMN) is an emerging disease for L. vannamei in Indonesia, first detected in May-June 2006, causing significant mortalities in grow-out ponds. The IMN is characterized by an acute onset of gross signs: focal to extensive whitish nec...
Journal of the World Aquaculture Society, 2016
Among the most common plants used by fish farmers in Java, 18 were selected for deeper ethnobotan... more Among the most common plants used by fish farmers in Java, 18 were selected for deeper ethnobotanical and laboratory investigation into their antimicrobial activity. These plants are mainly used against fish diseases, but with no precise therapeutic indication, leading to low specificity of use. Leaves, which were the most commonly used part of plants, were generally placed directly in water or less frequently added to the feed. Disk diffusion tests showed that 15 plants of the 18 (83%) displayed varying degrees of antibacterial activity and that Streptococcus agalactiae was more sensitive than Aeromonas hydrophila. Crude ethanolic extracts (EE) were more active than water macerates (WM), and minimum inhibitory concentrations (MICs) ranged from 12.5 to 25 mg/mL. Only the EE of Piper betle had an inhibitory effect against A. hydrophila. No MIC was found for any of the WM studied here. Furthermore, both the EE and, to a lesser extent, the WM of active plants were able to reduce bacterial growth by more than 99%, even at doses below their MICs. These results suggest that these plant extracts have a potential for eco-friendly prevention of bacterial fish diseases.
Media Akuakultur, 2009
Perkembangan vaksin pada ikan masih dalam tahap penelitian. Rencana strategis untuk pengembangan ... more Perkembangan vaksin pada ikan masih dalam tahap penelitian. Rencana strategis untuk pengembangan vaksin oleh peneliti merupakan pola dari riset yang dilakukan. Identifikasi antigen yang bersifat protektif, metode untuk produksi antigen protektif dalam kultur mikroba, metode bagaimana merubah bentuk antigen protektif menjadi bersifat imunogenik, merupakan tiga tahap yang dilakukan dalam riset tentang vaksin. Vaksin polivaleni adalah bentuk vaksin generasi baru yang memberikan perlindungan terhadap dua atau tiga penyakit pada saat yang sama jika dibandingkan dengan aplikasi satu atau dua vaksin secara terpisah. Keuntungan dari strategi ini adalah: sedikit jarum yang diinjeksikan, berkurangnya prosedur tata laksana, dan sumber daya manusia (SDM) yang digunakan. Dalam makalah ini juga dibahas keuntungan dan kerugian penggunaan vaksin polivalen serta tahap-tahap produksi vaksin tersebut.
Jurnal Penelitian Perikanan Indonesia, 2017
Sukses penanggulangan suatu penyakit akan banyak tergantung pada kecepatan dan ketepatan diagnosi... more Sukses penanggulangan suatu penyakit akan banyak tergantung pada kecepatan dan ketepatan diagnosisnya.
Journal of Aquaculture Science
Eel (Anguilla sp.) is one of the important commercial fish in some countries particularly in East... more Eel (Anguilla sp.) is one of the important commercial fish in some countries particularly in East Asian countries such as Japan, South Korea, China, and Taiwan. The need of eel fry or frequently called as glass eel still relies on the natural capture. The issue of natural captured fish is the high level of bacterial infections and diseases. The dependence of glass eel from the nature provides an indication of infections of parasites and bacteria in glass eel. The existence of parasites and bacteria as microorganisms which are potential as pathogenic agents in the cycle of eel culture can be a serious obstacle, so that the glass eel handling needs a particular attention since the capturing proses from the nature. Therefore, identification of parasites and bacteria in the capture location of glass eel was necessarily conducted in Pelabuhan Ratu Bay, Sukabumi, West Java. The glass eel sampling was conducted in May-June 2019, in 3 locations of estuary with the highest capture yield name...
Terapi herbal untuk penanggulangan penyakit ikan baik parasitik maupun bakterial telah banyak dig... more Terapi herbal untuk penanggulangan penyakit ikan baik parasitik maupun bakterial telah banyak digunakan oleh pembudidaya ikan sebagai alternatif obat ikan yang ramah lingkungan, serta dapat memanfaatkan kearifan lokal. Pembudidaya dalam menggunakan herbal sebagai desinfektan dan antiseptik terkadang tidak memperhatikan tingkat kerentanan masing-masing jenis ikan terhadap respon zat aktif dari setiap jenis herbal. Penelitian ini bertujuan untuk melihat tingkat kerentanan ikan Mas (Cyprinus carpio), Gurami (Osphronemus gouramy Lac.), Lele (Clarias gariepinus), Patin (Pangasius sp), dan Nila (Oreochromis niloticus) terhadap aplikasi desinfektan dan antiseptik herbal Medis-Herb MH-1 yang merupakan campuran dari Piper betle (daun sirih), Carica papaya (daun pepaya), Lithonia diversifolia (daun kipahit), Curcuma longa (kunyit), Psidium guajava (daun jambu biji), dan Morinda citrifolia L. (daun mengkudu). Ikan mas dan gurami ternyata lebih sensitif terhadap penggunaan herbal dibandingkan d...
Penelitian tentang diagnosa dan pengendalian penyakit "red legs" pada KODOK BENGGALA Ra... more Penelitian tentang diagnosa dan pengendalian penyakit "red legs" pada KODOK BENGGALA Rana catesbeiana Shaw telah dilakukan dengan tujuan untuk mengetahui genus dan prevalensi bakteri patogen dalam penyakit tersebut serta pengendaliannya secara kemoterapi.
Uploads
Papers by Taukhid Taukhid