International Journal of Vaccines & Vaccination, 2016
Background: Mass vaccination has significantly reduced the incidence of pertussis; however, the d... more Background: Mass vaccination has significantly reduced the incidence of pertussis; however, the disease is re-emerging even in some countries with high vaccination coverage. In Serbia, whole cell pertussis vaccine was introduced in 1957 and it has been used since 2014. Methods: To monitor changes in bacterial population, 77 isolates collected from 1953 to 2013 were studied. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn), pertussis toxin S1 subunit (ptxA) and pulsed-field gel electrophoresis analysis (PFGE). Results: Shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. In the period 1961-1979 genotype ptxA1 became as common as genotype ptxA2. After that, during the period of 1980-1989, the predominant ptx genotype was ptxA1. Reappearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981. The prn2 allele was only found in one strain isolated in 1984, two of the four strains isolated in 2000 and three strains from 2011. Serotype Fim2.3 disappeared before 1980 and serotype Fim2 became predominant since then. The four vaccine strains represented four PFGE profiles. Twenty-two (40%) isolates tested by PFGE produced 22 distinct profiles. Twenty-four (43%) isolates had unique Serbian profiles (BpSBR). Conclusion: The results of this present study indicate that the B. pertussis population in Serbia is different from other vaccinated populations and that this difference may be related to the vaccine used for 57years.
In Serbia, whole cell pertussis vaccine was introduced in 1957. Current composition of the vaccin... more In Serbia, whole cell pertussis vaccine was introduced in 1957. Current composition of the vaccine has been used since 1985 and contains four autochthonous strains of Bordetella pertussis isolated from 1957 to 1984. To monitor changes in bacterial population, 70 isolates collected from 1953 to 2000 were studied together with the vaccine strains. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn) and pertussis toxin S1 subunit (ptxA), and pulsed-field gel electrophoresis analysis. Shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. All isolates from 1980 to 1984 harbored ptxA1. Re-appearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981 and 1984. The allele prn2 was found only in two strains isolated in 2000. Serotype Fim2.3 disappeared before 1980 and serotype F...
Mass vaccination has significantly reduced the incidence of pertussis, however, the disease is re... more Mass vaccination has significantly reduced the incidence of pertussis, however, the disease is re-emerging, even in some countries with high vaccination coverage. In Serbia, whole cell pertussis vaccine was introduced in 1957. To monitor changes in bacterial population, 77 isolates collected from 1953 to 2011 were studied. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn) and pertussis toxin S1 subunit (ptxA). A shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. In the period 1961-1979, the genotype ptxA1 became as common as genotype ptxA2. After that, during the period 1980-1989, the predominant ptx genotype was ptxA1. The reappearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981. The prn2 allele was only found in one strain isolated in 1984, two of the four...
International Journal of Vaccines & Vaccination, 2016
Background: Mass vaccination has significantly reduced the incidence of pertussis; however, the d... more Background: Mass vaccination has significantly reduced the incidence of pertussis; however, the disease is re-emerging even in some countries with high vaccination coverage. In Serbia, whole cell pertussis vaccine was introduced in 1957 and it has been used since 2014. Methods: To monitor changes in bacterial population, 77 isolates collected from 1953 to 2013 were studied. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn), pertussis toxin S1 subunit (ptxA) and pulsed-field gel electrophoresis analysis (PFGE). Results: Shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. In the period 1961-1979 genotype ptxA1 became as common as genotype ptxA2. After that, during the period of 1980-1989, the predominant ptx genotype was ptxA1. Reappearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981. The prn2 allele was only found in one strain isolated in 1984, two of the four strains isolated in 2000 and three strains from 2011. Serotype Fim2.3 disappeared before 1980 and serotype Fim2 became predominant since then. The four vaccine strains represented four PFGE profiles. Twenty-two (40%) isolates tested by PFGE produced 22 distinct profiles. Twenty-four (43%) isolates had unique Serbian profiles (BpSBR). Conclusion: The results of this present study indicate that the B. pertussis population in Serbia is different from other vaccinated populations and that this difference may be related to the vaccine used for 57years.
In Serbia, whole cell pertussis vaccine was introduced in 1957. Current composition of the vaccin... more In Serbia, whole cell pertussis vaccine was introduced in 1957. Current composition of the vaccine has been used since 1985 and contains four autochthonous strains of Bordetella pertussis isolated from 1957 to 1984. To monitor changes in bacterial population, 70 isolates collected from 1953 to 2000 were studied together with the vaccine strains. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn) and pertussis toxin S1 subunit (ptxA), and pulsed-field gel electrophoresis analysis. Shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. All isolates from 1980 to 1984 harbored ptxA1. Re-appearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981 and 1984. The allele prn2 was found only in two strains isolated in 2000. Serotype Fim2.3 disappeared before 1980 and serotype F...
Mass vaccination has significantly reduced the incidence of pertussis, however, the disease is re... more Mass vaccination has significantly reduced the incidence of pertussis, however, the disease is re-emerging, even in some countries with high vaccination coverage. In Serbia, whole cell pertussis vaccine was introduced in 1957. To monitor changes in bacterial population, 77 isolates collected from 1953 to 2011 were studied. The methods included serotyping of fimbriae (Fim), genotyping of pertactin (prn) and pertussis toxin S1 subunit (ptxA). A shift from ptxA2 to ptxA1 has been observed in isolates since the late of 1960s. In the period 1961-1979, the genotype ptxA1 became as common as genotype ptxA2. After that, during the period 1980-1989, the predominant ptx genotype was ptxA1. The reappearance of the ptxA2 allele followed an addition of the two strains harboring ptxA1 in the vaccine in 1985. The allele prn1 was predominant among the Serbian isolates, though prn3 and prn11 have been detected since 1981. The prn2 allele was only found in one strain isolated in 1984, two of the four...
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