Papers by Hidayatullah Tak
Giardia is a common protozoan parasite infecting children in developing countries and is known to... more Giardia is a common protozoan parasite infecting children in developing countries and is known to result protein loss in infected children. The objective of this study was to assess the association of plasma protein profile with Giardiasis among children in district Anantnag of Kashmir valley. The study included 268 children aged 5-15 years belonging to both the genders. Stool samples collected from children were screened for Giardia infection and blood samples were analysed to estimate plasma protein, albumin and globulin levels. The results showed a significant decrease in mean values of total protein between infected and uninfected children from 6.71±0.96 to 7.01±0.98. The mean values of albumin also showed a decrease in their values between infected and uninfected children from 3.7±0.45 to 4.21±0.51 and that of globulin showed an increase in mean values from 3.01±0.91 to 2.8±0.58 between infected and uninfected children. However the difference was statistically insignificant (p&...
Necroscopic study of 284 goats was examined for Moniezia expansa Rudolphi, 1891 infection for the... more Necroscopic study of 284 goats was examined for Moniezia expansa Rudolphi, 1891 infection for the period of one year. The infection rate observed during the study was 2.11%. Histopathological study of the infected tissues with Moniezia expansa revealed shortened and flattened villi and local haemorrhages. The luminal site of the duodenum was found to b depressed like cavity because of Moniezia expansa.
Neotropical Helminthology, 2020
Las trematodiasis zoonóticas han surgido a un ritmo rápido y frecuente con un importante costo hu... more Las trematodiasis zoonóticas han surgido a un ritmo rápido y frecuente con un importante costo humano y financiero. La más importante y conocida zoonosis humana causada por trematodos, que afectan al hígado es la fasciolosis. El presente trabajo ha sido realizado para tener una revisión exhaustiva de la extensa bibliografía sobre la fasciolosis en detalle que está aumentando rápidamente en incidencia y alcance geográfico. Las razones para el aumento de la infección por Fasciola spp son probablemente multifactoriales. El uso de la parasitoproteómica como un poderoso enfoque experimental, y sus potenciales beneficios para la biología de trematodos también se examinan en relación con el control futuro de las infecciones por trematodos en animales y en seres humanos. El objetivo es hacer a los jóvenes investigadores más vigilantes sobre la importancia de esta temática crítica. Los esfuerzos mundiales para ampliar la planificación conjunta, el intercambio de información, y el apoyo finan...
An IgG antibody prevalence of 24.2% (123/508) was found in the study population, while examinatio... more An IgG antibody prevalence of 24.2% (123/508) was found in the study population, while examination of specific IgA and IgM antibodies was negative. A significant increase in toxoplasmosis prevalence with increasing age was confirmed (p =0.001). There were no significant differences between genders or between inhabitants of rural and urban areas (p =0.079 and p =0.291, respectively). An increased consumption of raw meat (40.7%) and raw vegetables or fruit (30.8%) was observed in serologically positive women. This difference between serologically positive and negative women was significant (p =0.042). A significant difference between men and women eating raw vegetables and fruit was also found (p =0.049). There was no difference in clinical symptoms between serologically positive and negative patients (p =0.658). Lymphadenopathy with or without other symptoms (influenza, arthralgia, ocular symptom, dermatosis), 40.7%, was the most common clinical symptom in seropositive patients.
Fasciolosis is emerging as a serious medical and veterinary problem. Etiological agent for this e... more Fasciolosis is emerging as a serious medical and veterinary problem. Etiological agent for this economically important zoonotic disease are Fasciola hepatica and Fasciola gigantica which coexist in bovine hosts reared in Kashmir province because of overlap in the boundaries of their respective intermediate snail hosts. But there is predominance of Fasciola gigantica which incur heavy losses in bovine rearing industry. Present investigation was carried out for detection of Fasciola gigantica soluble antigens readily diagnosed by Fasciola gigantica infected bovine sera using Western blot analysis.
Journal of Veterinary Advances, 2016
The present study of seroprevalence of B. bigemina and A. marginale in cattle, sheep and goats wa... more The present study of seroprevalence of B. bigemina and A. marginale in cattle, sheep and goats was studied in Ganderbal district of Kashmir, between January to December 2012. A total of 153 blood samples were collected randomly from 40 cattle, 52 sheep and 61 goats for the preparation of blood smears and serum samples in four consecutive seasons and tested against B. bigemina and A. marginale using the SVANOVIR ® B. bigemina -Abs and A. marginale -Abs ELISA Kit. Samples were also examined by Giemsa's stained blood smear method. The effect of topography, season, age, gender and breed was observed in cattle during this study. The overall prevalence of B. bigemina infection was 3 (7.5%), 2 (3.84%) and 3 (4.91%) in cattle, sheep and goats and for A. marginale 2 (5%), 1 (1.92%) and1 (1.63%) respectively. The mixed infections between B. bigemina and A. marginale were 5% in cattle, 1.92% in sheep and 3.27% in goats. The seasonal prevalence of B.bigemina , A. marginale and mixed infecti...
International Journal of Scientific & Technology Research, 2020
Coprological analysis was conduction during February, 2017 to January, 2019 to evaluate the preva... more Coprological analysis was conduction during February, 2017 to January, 2019 to evaluate the prevalence of gastrointestinal helminths in stray cats of Kashmir valley, India. Fecal floatation and sedimentation technique revealed helminths in 349 scats out of 887 scats, with an overall prevalence of 39.43%. The study revealed four species of helminths in scats. Cats harboring one helminth were (6.31%), those harboring two were common (19.16%), than three (13.30%) or four (0.5%). The helminths observed and their respective frequencies detected were: Toxocara cati (26.49%), Dipylidium caninum (19.39%), Spirometra spp. (18.15%) and Taenia spp. (22.50%). Toxocara cati, a nematode with great zoonotic significance, was most common helminth found in scats of cat during the study. We observed significant seasonal variation (p<0.05) of prevalence in the gastrointestinal helminths, with high prevalence during summer followed by autumn, spring and least in winter. The high prevalence of zoonot...
Journal of Medical Sciences, 2017
is an International, peer-reviewed scientific journal that publishes original article in experime... more is an International, peer-reviewed scientific journal that publishes original article in experimental & clinical medicine and related disciplines such as molecular biology, biochemistry, genetics, biophysics, bio-and medical technology. JMS is issued four times per year on paper and in electronic format.
The present study was conducted for the time period of one year on goats of Ladakh (J&K) with the... more The present study was conducted for the time period of one year on goats of Ladakh (J&K) with the aim to find out the various nematode parasites infesting goats of this region and their prevalence with regard to season, sex, age, body condition, and agro-ecology. A total of 268 gastro intestinal tracts along with heart and lungs of the host animal of either sex and of different age groups belonging to different areas of the study area were collected randomly and were examined for nematode parasites. Out of these 116 (43.28%) were found infected with single or multiple parasite species. The study reveals the presence of four species of nematodes viz; Trichuris ovis, Haemonchus contortus, Dictyocaulus filaria and Chabertia ovina. It was also observed that among these T. ovis (48.03%) was most dominant followed by H. contortus (41.02%), D. filaria (37.05%) and C. ovina (22.35%) respectively. A significant difference was observed in prevalence of nematode parasites with respect to seaso...
Journal of Parasitic Diseases, 2014
The present study aims to determine the agewise, sex-wise and month-wise prevalence along with se... more The present study aims to determine the agewise, sex-wise and month-wise prevalence along with seasonal fluctuations of Trichuris spp. in ovines and caprines slaughtered during a 12 month period in local abattoirs in Srinagar region from August 2011 to July 2012. Adult parasites were identified on the basis of morphological characters (Soulsby Helminths, arthropods and protozoa of domes-229ticated animals, CLBS and Bailliere Tinda, London,1982). The highest prevalence (66.6 %) was in the month of Jan, 2012 whereas prevalence was lowest in the month of August 2011. Trichuris count in ovines increased in autumn (42.02 %), reached maximum levels in winter (59.37), and then tended to decline until spring (53.22 %) and reached minimum levels in summer (30.6 %), before increasing again in mid-autumn. Thus with respect to climatic conditions of area from which exotic ovines were imported, Trichuris prevalence was more prevalent in dry season(55.5 %) than in wet season (36.36 %). Moreover, an association was observed between sex and age of the host with prevalence of Trichuris infection. Of the representative examined samples, Trichuris infection was 44.07 % in female host comparative to 38.07 % infection in males (p [ 0.05). Likewise young animals were more infected (53.8 %) than the adult ones (32.9 %) and kids (37.5 %). Moreover, Trichuris spp. were more prevalent in goats than in sheep(p \ 0.05). Hence, it was concluded that prevalence of Trichuris spp. infecting ovines varied with respect to season, age and sex.
Liver, 2012
... Azra N. Kamili, Centre of Research for Development, University of Kashmir, Srinagar for provi... more ... Azra N. Kamili, Centre of Research for Development, University of Kashmir, Srinagar for providing technical support during present work. ... 8. Pandit, BA, RA Shahardar, MM Darzi, MAA Banday and AS Bhat, 2003 Survey of Gastrointestinal nematodes in sheep of Kashmir Valley. ...
Journal of Parasitic Diseases, 2014
Gastrointestinal trichostrongyles of small ruminants are one of the major causes of productivity ... more Gastrointestinal trichostrongyles of small ruminants are one of the major causes of productivity loss. Epidemiological study was carried out to determine parasitic infection of sheep with abamossal nematodes at various abattoirs in Srinagar district of Kashmir Valley from August 2011 to July 2012. On the basis of necroscopy, out of representative 281 abamossa, 53.3 % were recorded to be infected with Haemonchus species and 41.2 % with Ostertagia spp. Thus, Haemonchus spp. were more prevalent than Ostertagia spp. in ovines (P [ 0.05). Infection prevalence percentage of Haemonchus spp. was highest in late summer season and early rainy season (62.85 %) with peak value in the month of July (71.42 %) and lowest in winter (42.85 %) with minimum value in the month of February (40 %). Similar trend was seen with Ostertagia spp. having highest infection prevalence value during summer season (52.8 %) with peak values in the month of July (64.2 %) and lowest infection in winter (34.2 %) with minimum value in February (30 %). Moreover, non-local breeds were more prevalent than local ones (P = 0.05).
The gastrointestinal helminths are known to have high potential for transmission through feco-ora... more The gastrointestinal helminths are known to have high potential for transmission through feco-oral route and may transmit from livestock to wild ungulates with an impact on their health and reproduction. We lack studies on prevalence of gastrointestinal helminth parasites in domestic livestocksharing habitat with wild ungulates of Hirpora Wildlife Sanctuary. As the domestic sheep and goat constitute more than 90% of livestock population, we therefore, collected faecal samples of domestic sheep (n=281) and goat (n= 226) in the sanctuary from June to October of two consecutive years (2018 and 2019) for qualitative examination of gastrointestinal helminth parasites by centrifugal sedimentation and floatation methods.The present study reveals theprevalence of 77.22% and 72.12% of gastrointestinal helminth parasites in domestic sheep and goat respectively with an overall prevalence of 74.67%. A total of seven species of gastrointestinal helminth parasites were recorded which are arranged...
Paramphistomum cervi is one of the most common trematode parasites infecting ruminants across the... more Paramphistomum cervi is one of the most common trematode parasites infecting ruminants across the globe. The current study was carried out to identify the somatic antigens present in P. cervi using SDS PAGE. The electrophoretic pattern of somatic proteins of P. cervi under reducing conditions in 12% SDS revealed the presence of several bands with the molecular weights ranging between 10-150 kDa. The major bands appeared at 10, 18, 23, 40, 50, 75 kDa. The proteins can be subjected to further experimental analysis for detection of their possible role in serodiagnosis or vaccine development.
Pakistan Journal of Zoology, 2012
Haemonchus contortus has posed a great problem to researchers and has created confusion among the... more Haemonchus contortus has posed a great problem to researchers and has created confusion among them regarding its identification. In this study an attempt has been made to study the morphology and morphometry of Haemonchus contortus recovered from the ruminants and to relate it with some parameters like study area, host, intensity, methodology, age and sex of the host. It was observed that all these factors have some minor intraspecific effects on the morphology and morphometry of the parasites. These variations were of minor importance because they were found within the range of similarity and were not good enough to label it as a new species as was expected. Also some of the parameters with regard to morphology and morphometry of this parasite were described for the first time which were not described by the previous authors and these could be of great taxonomic importance for the identification of this parasite which is being confused many times and is being labeled a new species.
Plants are the source of large amount of drugs comprising to different groups such as antispasmod... more Plants are the source of large amount of drugs comprising to different groups such as antispasmodics, emetics, anti-cancer, antimicrobials etc. A large number of the plants are claimed to possess the antibiotic properties in the traditional system and are also used extensively by the tribal people worldwide. It is now believed that nature has given the cure of every disease in one way or another. Plants have been known to relieve various diseases in Ayurveda. Therefore, the researchers today are emphasizing on evaluation and characterization of various plants and plant constituents against a number of diseases based on their traditional claims of the plants given in Ayurveda. In this present review, an attempt has been made to give an overview of certain bioactive compounds which may have the antimicrobial activity.
The variation in electrophoretic patterns between two economically important species of Fasciolai... more The variation in electrophoretic patterns between two economically important species of Fasciolais important tools for identification of these species and also for vaccine designing. The aim behind the review is to encourage more young researchers to initiate work on this aspect of an economically cosmopolitan parasite. Disease biomarker discovery is generally carried out using two dimensional polyacrylamide gel Electrophoresis (2D-PAGE) to compare and identify differences in the protein expression patterns of two parasites. After 2D-PAGE fractionation and staining, the protein(s) of interest are removed, proteolytically or chemically digested and identified by mass spectrometry (MS). Although 2DPAGE separation provides excellent resolution, the need for protein staining and the subsequent sample handling limits the sensitivity of the overall approach. Protein profiling is expected to discover unexpected targets for drug design by determining the function of thousands of unidentifie...
Neotropical helminthology, 2016
Las trematodiasis zoonoticas han surgido a un ritmo rapido y frecuente con un importante costo hu... more Las trematodiasis zoonoticas han surgido a un ritmo rapido y frecuente con un importante costo humano y financiero. La mas importante y conocida zoonosis humana causada por trematodos, que afectan al higado es la fasciolosis. El presente trabajo ha sido realizado para tener una revision exhaustiva de la extensa bibliografia sobre la fasciolosis en detalle que esta aumentando rapidamente en incidencia y alcance geografico. Las razones para el aumento de la infeccion por Fasciola spp son probablemente multifactoriales. El uso de la parasitoproteomica como un poderoso enfoque experimental, y sus potenciales beneficios para la biologia de trematodos tambien se examinan en relacion con el control futuro de las infecciones por trematodos en animales y en seres humanos. El objetivo es hacer a los jovenes investigadores mas vigilantes sobre la importancia de esta tematica critica. Los esfuerzos mundiales para ampliar la planificacion conjunta, el intercambio de informacion, y el apoyo finan...
The Journal of medical research, 2016
Fasciolosis is denoted as a significant veterinary health problem. During current study, a total ... more Fasciolosis is denoted as a significant veterinary health problem. During current study, a total of 714 cattle slaughtered at different abattoirs of Srinagar city (J&K) were examined for the presence of Fasciola sps in the liver from January 2014 to January 2016. There was moderate prevalence of 26.84% in the studied area. Predominance of Fasciola gigantica (20.86%) was seen as compared to Fasciola hepatica (3.361%) infection with mixed infection of 2.66%. Epidemiological determinants like age, gender, breed and body condition showed statistically significant (p<0.05) effect on bovine Fasciolosis. Seasonal data showed highest prevalence in autumn (39.87%) followed by winter (28.84%) with lowest prevalence in spring (16.40%).
Journal of Threatened Taxa, 2021
A one-year study conducted to ascertain the prevalence and risk factors associated with helminth ... more A one-year study conducted to ascertain the prevalence and risk factors associated with helminth infection in Hangul Deer Cervus hanglu hanglu at Dachigam National Park revealed that 40.45% (89 of 220 samples) were infected with four helminth species including Heamonchus contortus, Trichuris ovis, Dictyocaulus viviparus, and Moneizia expansa. The study signified that the infection was more prevalent during the dry season (summer and autumn) as compared to the wet season (winter and spring). The overall prevalence of Heamonchus contortus was the highest (23.18%) followed by Trichuris ovis (8.18%), followed by Dictyocaulus viviparus (5.45%), and Moneizia expansa (3.63%). The present study should be of importance in conserving the erstwhile state animal, listed as Critically Engendered in 2017 by IUCN.
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Papers by Hidayatullah Tak