Papers by Suzanne Michiko Bon
European Journal of Biochemistry, 1978
European Journal of Biochemistry, 1978
European Journal of Biochemistry, 1978
European Journal of Biochemistry, 1976
Molecular Biology Reports, 1978
Acetylcholinesterase from Electrophorus electric organs may be readily solubilized, in the form&#... more Acetylcholinesterase from Electrophorus electric organs may be readily solubilized, in the form' of asymmetrical molecules (9, 10) containing a rod-like tail (4, 5, 12). These molecules undergo a reversible aggregation process in low ionic strength conditions (3, 7, 9), forming aggregates which sediment around 70 S. These aggregates consist of about half a dozen molecules, the tails of which are packed side-to-side in bundles, leaving the head-moieties at both extremities of the structure. We describe here another multimolecular association of acetylcholinesterase molecules, which differs from the low ionic strength aggregates by its lower sedimentation coefficient, and which is not sensitive to ionic conditions. Instead, covalent disulfide bonds seem to be responsible for its stability since it is disrupted by reducing agents. Electron microscopy revealed a dumbbell structure which was identical to that already observed by Dudai et al. in some of their acetylcholinesterase preparations (5).
Annual Review of Neuroscience, 1982
The mechanism of cholinergic neurotransmission requires the rapid inacн tivation of acetylcholine... more The mechanism of cholinergic neurotransmission requires the rapid inacн tivation of acetylcholine (Dale 1914). Loewi & Navratil showed in 1926 that acetylcholine can be destroyed by an enzyme that exists in aqueous extracts of frog tissues. An esterase that specifically hydrolyzes ...
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Papers by Suzanne Michiko Bon