Papers by Sompong Doolgindachbaporn
Journal of Pure and Applied Microbiology, Sep 30, 2017
May to December 2015 to justify effects of chitosan of Experiment 1 on growth performance of rice... more May to December 2015 to justify effects of chitosan of Experiment 1 on growth performance of ricefield crabs. The Experiment 1 had five chitosan treatments, i.e. 0, 1, 2, 3 and 4 % (on dry matter basis). Experiment 2 had two treatments, i.e. the best treatment of Experiment 1 (T3) was chosen in comparison with flesh fish meat treatment. A Completely Randomized Design (CRD) with four replications was used for Experiments 1 and 2. The results of the Experiment 1 showed that a chitosan level of 2 % of T3 gave significantly (p < 0.05) better growth performance on final weight gained, weight gained, average daily growth rate, specific growth rate and survival rate (%) of the ricefield crabs than the rest, except that of the feed conversion ratio where T1 (control) was the highest. Thus a chitosan level of 2 % (T3) in the feed diet is recommended for ricefield crab culture. With the Experiment 2, the feed diet of T3 of Experiment 1, i.e. with 2 % chitosan gave significantly (p < 0.05) higher survival rate (%) of ricefield crabs than the flesh fish meat of T1.
Fish & Shellfish Immunology, Nov 1, 2017
Effects of Astragalus polysaccharides (APS) and chitooligosaccharides (COS) on growth, immune res... more Effects of Astragalus polysaccharides (APS) and chitooligosaccharides (COS) on growth, immune response and disease resistance of juvenile largemouth bass, Micropterus salmoides
Aquaculture Research, Sep 28, 2021
Pakistan Journal of Biological Sciences, Dec 15, 2010
Fish Physiology and Biochemistry, Nov 16, 2017
The study was conducted to evaluate the effect of dietary phospholipids (PLs) on growth, lipid me... more The study was conducted to evaluate the effect of dietary phospholipids (PLs) on growth, lipid metabolism, and antioxidative status of hybrid snakehead (Channa argus × Channa maculata). Five isonitrogenous and isolipidic diets with graded levels of PLs (8.5, 19.3, 30.7, 41.5, and 50.8 g kg −1) were fed to triplicate groups of juveniles (initial body weight 12.6 ± 0.23 g) for 8 weeks. Results showed that dietary PL supplementation significantly improved growth of juveniles. The final body weight (FBW) and specific growth rate (SGR) significantly increased with dietary PLs increasing from 8.5 to 41.5 g kg −1 (P < 0.05). Fish fed with the diet containing 8.5 g kg −1 PLs showed higher feed conversion ratio (FCR) compared to the other treatments (P < 0.05). Survival rate (SR) was not affected by dietary PL levels (P > 0.05). Liver lipid contents, serum triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) contents significantly decreased with the increasing levels of dietary PLs (P < 0.05). However, serum total cholesterol (TC) and highdensity lipoprotein cholesterol (HDL-C) contents and HDL-C/TC and HDL-C/LDL-C value significantly increased with increasing dietary PL levels (P < 0.05). The catalase (CAT), superoxide dismutase (SOD), and carnitine palmitoyl transferase I (CPT-1) activities in the liver significantly increased with incremental dietary PL level (P < 0.05), while the liver malondialdehyde (MDA) contents and fatty acid synthase (FAS) activity significantly reduced (P < 0.05). No significant difference was observed in the glutathione peroxidase (GPx) activity among dietary treatments (P > 0.05).These results confirmed that dietary PL supplementation has beneficial effects on growth performance and antioxidant capacity of juvenile hybrid snakehead. Dietary PLs might reduce lipid deposition in the liver of juvenile hybrid snakehead.
Molecular Biology Reports, Dec 18, 2011
Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used... more Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to investigate the genetic structure of four subpopulations of Mystus nemurus in Thailand. The 7 RAPD and 7 ISSR primers were selected. Of 83 total RAPD fragments, 80 (96.39%) were polymorphic loci, and of 81 total ISSR fragments, 75 (92.59%) were polymorphic loci. Genetic variation and genetic differentiation obtained from RAPD fragments or ISSR fragments showed similar results. Percentage of polymorphic loci (%P), observed number of alleles, effective number of alleles, Nei's gene diversity (H) and Shannon's information index revealed moderate to high level of genetic variations within each M. nemurus subpopulation and overall population. High levels of genetic differentiations were received from pairwise unbiased genetic distance (D) and coefficient of differentiation. Mantel test between D or gene flow and geographical distance showed a low to moderate correlation. Analysis of molecular variance indicated that variations among subpopulations were higher than those within subpopulations. The UPGMA dendrograms, based on RAPD and ISSR, showing the genetic relationship among subpopulations are grouped into three clusters; Songkhla (SK) subpopulation was separated from the other subpopulations. The candidate species-specific and subpopulation-specific RAPD fragments were sequenced and used to design sequencecharacterized amplified region primers which distinguished M. nemurus from other species and divided SK subpopulation from the other subpopulations. The markers used in this study should be useful for breeding programs and future aquacultural development of this species in Thailand.
Fish & Shellfish Immunology, Dec 1, 2014
This study evaluated effects of low molecular weight agar (LMWA) and Lactobacillus plantarum sing... more This study evaluated effects of low molecular weight agar (LMWA) and Lactobacillus plantarum singly or combined on growth performance, immunity and disease resistance of basa fish (Pangasius bocourti). Two hundred forty fish were divided into four treatments, i.e. 0 g kg(-1) LMWA (Control), 2 g kg(-1) LMWA, 10(8) cfu g(-1)L. plantarum, and 2 g kg(-1) LMWA + 10(8) cfu g(-1)L. plantarum. Following 7, 14 and 28 days of the treatment, specific growth rate (SGR), feed conversion ratio (FCR), serum lysozyme, phagocytosis, respiratory burst and alternative complement activity (ACP) were measured. A Completely Randomized Design with four replications was applied. At the end of the feeding trial, five fish were randomly selected for a challenge test against Aeromonas hydrophila. The results showed that fish fed diet of 2 g kg(-1) LMWA and 10(8) cfu g(-1) of L. plantarum singly or combined significantly enhanced SGR, FCR, serum lysozyme, phagocytosis, respiratory burst, alternative complement activities and post-challenge survival rate of P. bocourti. The results inferred that dietary of LMWA and L. plantarum stimulated growth, immunity and disease resistance of the P. bocourti.
Pakistan Journal of Biological Sciences, May 1, 2015
Thai Journal of Agricultural Science, 2017
In Malaysia, the giant freshwater prawn Macrobrachium rosenbergii is becoming an increasingly imp... more In Malaysia, the giant freshwater prawn Macrobrachium rosenbergii is becoming an increasingly important targeted species, as its culture, is considered to have the potential to raise income among impoverished farmers. Although, total freshwater aquaculture production decreased in the year 2013 compared to previous year, the aquaculture production of M. rosenbergii increased from 318 tonnes in 2012 to 457 tonnes in 2013. Recently, the production of giant freshwater fries increased from the three government and 21 private hatcheries in 2012 to the four government and 19 private hatcheries in 2013. The number and production of feed mills increased in 2013 compared to the four years ago. Until recently, lack of a stable nursery of PL and feed had been an important obstacle to the further expansion and development of M. rosenbergii culture. This paper reviews the current status of freshwater prawn culture in Malaysia and background history, and future prospects of freshwater prawn farming. It was concluded that freshwater prawn farming in Malaysia has a favorable scenario for expansion due to increasing demand and to prospects of an improved organization of the productive chain.
Fish Physiology and Biochemistry, Apr 29, 2016
This study was conducted to evaluate the effects of Eryngii mushroom, Pleurotus eryngii (PE), and... more This study was conducted to evaluate the effects of Eryngii mushroom, Pleurotus eryngii (PE), and Lactobacillus plantarum single or combined on growth, innate immune response and disease resistance of the Pangasius catfish, Pangasius bocourti. Two hundred forty fish were divided into four treatments, i.e., 0 g kg-1 PE (Control, Diet 1), 3 g kg-1 PE (Diet 2), 10 8 cfu g-1 L. plantarum (Diet 3) and 3 g kg-1 PE ? 10 8 cfu g-1 L. plantarum (Diet 4). Fish were culture in glass tanks with water volume approximately of 150 l, and each treatment had four replications with 15 fish per replication. Following 30, 60 and 90 days of the feeding trial, specific growth rate (SGR), feed conversion ratio (FCR), serum lysozyme, phagocytosis and respiratory burst activities were measured. SGR and FCR were significantly improved in fish fed supplemented diets after 90 days of the feeding trial. Serum lysozyme, phagocytosis and respiratory burst activity of fish were significantly stimulated by both PE and L. plantarum diets; however, the highest innate immune response was observed in fish fed synbiotic diet. At the end of the experiment, five fish were randomly selected for a challenge test against Aeromonas hydrophila. The post-challenge survival rate of the fish fed supplemented diets was significantly greater than the control treatment, and the highest post-challenge survival rate was observed in synbiotic diet. The results revealed that dietary supplementation of PE and L. plantarum stimulated growth, immunity and disease resistance of the P. bocourti.
Aquaculture Nutrition, Feb 2, 2015
ABSTRACT This study evaluated the effects of Jerusalem artichoke (JA) and Lactobacillus plantarum... more ABSTRACT This study evaluated the effects of Jerusalem artichoke (JA) and Lactobacillus plantarum singly or combined on growth, immunity and disease resistance of Pangasius bocourti. In the first experiment, different concentrations of JA (0, 5, 10, 20, 40, 80 and 160 g kg−1) were administered to determine an optimal concentration on growth of P. bocourti. In the second experiment, the optimal concentration of JA (5 g kg−1) was combined with 108 cfu g−1 L. plantarum. In the final experiment, five randomly selected fish from the second experiment were challenged with Aeromonas hydrophila. Treatments for second and third experiments were 0 g kg−1 JA (Diet 1), 5 g kg−1 JA (Diet 2), 108 cfu g−1L. plantarum (Diet 3) and 5 g kg−1 JA + 108 cfu g−1L. plantarum (Diet 4). Fish fed 5 g kg−1 JA or 108 cfu g−1 of L. plantarum significantly improved specific growth rate (SGR), feed conversion ratio (FCR), serum lysozyme activity and postchallenge survival rate (PCSR). Dietary in the combination of JA and L. plantarum showed significantly enhanced SGR, FCR, serum lysozyme, phagocytosis, respiratory burst activities and PCSR compared with control and individual applications. Dietary JA and L. plantarum significantly stimulated growth, immunity and disease resistance of P. bocourti.
Khon Kaen University Journal (Graduate Studies), 2009
In this study, 119 bacterial strains were isolated from various samples such as healthy tilapia f... more In this study, 119 bacterial strains were isolated from various samples such as healthy tilapia fish (Oreochromis niloticus), water and sediment around the culture fish-cages, and several kinds of traditional fermented foods. These bacterial isolates were screened for antibacterial activities against bacterial fish pathogens i.e. Aeromonas hydrophila, A. caviae and Streptococcus agalactiae using an agar well diffusion assay. The isolate CR1T5 derived from fermented rice showed the highest antibacterial activity against all three fish pathogens tested. It was identified as Lactobacillus plantarum by using both conventional and molecular methods. The other probiotic properties were evaluated in vitro which revealed that strain CR1T5 tolerated the simulated gastrointestinal conditions well, showed high capacity to adhere intestinal mucosa and did not lyse red blood cells. The efficiency of L. plantarum CR1T5 was also examined in vivo. O. niloticus were employed in the feed-trial experi...
Journal of Pure and Applied Microbiology
The effects of a probiotic Lactobacillus plantarum CR1T5, at various concentrations(0, 10 6 , 10 ... more The effects of a probiotic Lactobacillus plantarum CR1T5, at various concentrations(0, 10 6 , 10 7 , 10 8 and 10 9 cfu g-1) in dietary supplements for Black Eared Catfish (Pangasius larnaudii) were investigated. Fish were randomly allocated into 5 treatments with 3 replications and reared for 60 days. The growth performance, non-specific immunity and disease resistance were determined at 15, 30, 45 and 60 days. The results revealed that at day 15 the fish received probiotic dietary supplements at 10 7 , 10 8 and 10 9 cfu g-1 had significantly higher Specific growth rate (SGR) and lower feed conversion ratio (FCR) than the fish received control diet. On the other hand, fish fed 10 6 cfu g-1 dietary supplement had significantly higher SGR and lower FCR at day 45. For immune parameters, the respiratory burst activity was significantly increased after 30 days. Probiotic diets at 10 8 and 10 9 cfu g-1 significantly improved complement activity after 15 days and significantly enhanced lysozyme activity after 45 days of feeding. All fish received probiotic dietary supplement displayed a significantly increased survival rate post-challenge with A. hydrophila. This study found that the optimum probiotic dietary supplement at 10 8 cfu g-1 had significantly improved growth performance, immune stimulation and disease resistance in P. larnaudii.
Fish & Shellfish Immunology
Journal of Pure and Applied Microbiology
Uploads
Papers by Sompong Doolgindachbaporn