Murine fibroblasts deficient in mitochondria respiratory complexes III (CIII) and IV (CIV) produc... more Murine fibroblasts deficient in mitochondria respiratory complexes III (CIII) and IV (CIV) produced by either the ablation of Uqcrfs1 (encoding for Rieske iron sulfur protein, RISP) or Cox10 (encoding for protoheme IX farnesyltransferase, COX10) genes, respectively, showed a pleiotropic effect in complex I (CI). Exposure to 1–5% oxygen increased the levels of CI in both RISP and COX10 KO fibroblasts. De novo assembly of the respiratory complexes occurred at a faster rate and to higher levels in 1% oxygen compared to normoxia in both RISP and COX10 KO fibroblasts. Hypoxia did not affect the levels of assembly of CIII in the COX10 KO fibroblasts nor abrogated the genetic defect impairing CIV assembly. Mitochondrial signaling involving reactive oxygen species (ROS) has been implicated as necessary for HIF-1α stabilization in hypoxia. We did not observe increased ROS production in hypoxia. Exposure to low oxygen levels stabilized HIF-1α and increased CI levels in RISP and COX10 KO fibro...
Background. We aimed to develop a quality-oflife subscale for nasopharyngeal carcinoma (NPC) and ... more Background. We aimed to develop a quality-oflife subscale for nasopharyngeal carcinoma (NPC) and validate the functional assessment of cancer therapy-nasopharyngeal (FACT-NP). Methods. The FACT-NP was tested cross-sectionally in survivors of postirradiated NPC (n ¼ 357) and was administered to newly diagnosed patients (n ¼ 160) before, at the end of, and 3 months after radiotherapy (RT). Results. Each FACT-NP domain was internally consistent (Cronbach's alpha ¼ 0.87-0.90). The test-retest reliability for each subscale was satisfactory (intraclass correlation coefficient ¼ .73-.88). Concurrent validity was suggested by the moderate to strong correlations between the FACT-NP and the Quality of Life-Radiation Therapy Instrument-Head and Neck (QOL-RTI-H&N) subscales (Pearson r ¼ .39-.84). The FACT-NP was responsive to clinical changes from pretreatment to 3 months after RT (effect sizes > 0.6 for clinically relevant subscales). The pooled data for multitrait scaling analysis showed satisfactory item internal consistency and item discriminant validity (100% and 90% scaling success, respectively). Conclusion. The FACT-NP is a reliable and valid instrument for measuring QOL in patients with NPC. V
Murine fibroblasts deficient in mitochondria respiratory complexes III (CIII) and IV (CIV) produc... more Murine fibroblasts deficient in mitochondria respiratory complexes III (CIII) and IV (CIV) produced by either the ablation of Uqcrfs1 (encoding for Rieske iron sulfur protein, RISP) or Cox10 (encoding for protoheme IX farnesyltransferase, COX10) genes, respectively, showed a pleiotropic effect in complex I (CI). Exposure to 1–5% oxygen increased the levels of CI in both RISP and COX10 KO fibroblasts. De novo assembly of the respiratory complexes occurred at a faster rate and to higher levels in 1% oxygen compared to normoxia in both RISP and COX10 KO fibroblasts. Hypoxia did not affect the levels of assembly of CIII in the COX10 KO fibroblasts nor abrogated the genetic defect impairing CIV assembly. Mitochondrial signaling involving reactive oxygen species (ROS) has been implicated as necessary for HIF-1α stabilization in hypoxia. We did not observe increased ROS production in hypoxia. Exposure to low oxygen levels stabilized HIF-1α and increased CI levels in RISP and COX10 KO fibro...
Background. We aimed to develop a quality-oflife subscale for nasopharyngeal carcinoma (NPC) and ... more Background. We aimed to develop a quality-oflife subscale for nasopharyngeal carcinoma (NPC) and validate the functional assessment of cancer therapy-nasopharyngeal (FACT-NP). Methods. The FACT-NP was tested cross-sectionally in survivors of postirradiated NPC (n ¼ 357) and was administered to newly diagnosed patients (n ¼ 160) before, at the end of, and 3 months after radiotherapy (RT). Results. Each FACT-NP domain was internally consistent (Cronbach's alpha ¼ 0.87-0.90). The test-retest reliability for each subscale was satisfactory (intraclass correlation coefficient ¼ .73-.88). Concurrent validity was suggested by the moderate to strong correlations between the FACT-NP and the Quality of Life-Radiation Therapy Instrument-Head and Neck (QOL-RTI-H&N) subscales (Pearson r ¼ .39-.84). The FACT-NP was responsive to clinical changes from pretreatment to 3 months after RT (effect sizes > 0.6 for clinically relevant subscales). The pooled data for multitrait scaling analysis showed satisfactory item internal consistency and item discriminant validity (100% and 90% scaling success, respectively). Conclusion. The FACT-NP is a reliable and valid instrument for measuring QOL in patients with NPC. V
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Papers by Sofia Garcia