Infertility in cows is one of the major problems with great economic implications. The purpose of... more Infertility in cows is one of the major problems with great economic implications. The purpose of this study was to identify by global and individual gynecological investigation major ovarian diseases and to determinate their incidence in the relation with hot season at Aberdeen Angus cows. The study was carried out in June 2019 in private unit in Timis county, Romania, on a number of 93 Aberdeen Angus cows in order to determine the prevalence of different ovarian diseases at this breed in a summer season. Gynecological investigations were performed in order to establish the prevalence of ovarian diseases in the summer season. The results showed different pathological entity variation. Thus, 50.22% of the animals taken into study presented ovarian hypofunction, 27.9 % presented corpus luteum , 0.93% polycystic ovary with uterine adherences and 7.44% ovarian follicle
”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2019
The objective of this study was to evaluate the effects of prostaglandins (natural and synthetic ... more The objective of this study was to evaluate the effects of prostaglandins (natural and synthetic analogues) on estrous synchronization in beef cows. The study was carried out between May-August 2019 in a private unit in Timis County, and involved 54 Aberdeen Angus cows diagnosed with ovarian luteal tissue: corpus luteum (n=36 cows), cystic corpus luteum (n= 8 cows) and luteal cysts (n=10 cows). Two products were used for the treatment of this pathology: PGF Veyx Forte (n=27 cows) and Enzaprost (n=27 cows). The interval between hormonal treatment and the onset of estrous was ranging from 48-96 hours. In this trial 83.33% of the animals expressed signs of estrous. In the present experiment the conception rate after the artificial insemination was 71.11%.
Lucrări Științifice - Medicină Veterinară, Universitatea de Științe Agricole și Medicină Veterinară "Ion Ionescu de la Brad" Iași, 2019
The purpose of this research was to evaluate an intrauterine suspension in the treatment of retai... more The purpose of this research was to evaluate an intrauterine suspension in the treatment of retained placenta. A therapeutic option that affects, as little as possible the female reproductive life, thus reducing the associated economic implication is the ideal in the treatment of retained placenta. In this study, we analyzed the cases of retained placenta in a farm from Cluj county, the incidence, the diagnosis and the treatment of this pathology in cows. The study was carried out during January 2017-April 2019 and included 216 animals that have calved in that period. Of these, 16 animals were with retained placenta at 12-15 hours after calving, which represents 7.40%. After the treatment, the service period, the number of doses of semen needed for one gestation and the costs of treatment were established.
Lucrări Științifice - Medicină Veterinară, Universitatea de Științe Agricole și Medicină Veterinară "Ion Ionescu de la Brad" Iași, 2017
The purpose of this work was to supervise the activity and health of the mammary gland in dairy c... more The purpose of this work was to supervise the activity and health of the mammary gland in dairy cows in a dairy specialty unit in Cluj County. The objectives of this study can be summarized as follows: diagnosis of mastitis, carrying out a study on the incidence of mastitis according to some factors, performing the microbiological exam and treatment. Following the clinical examination, 6 cows (5.36%) were diagnosed with clinical mastitis. Depending on the type of lesion, it was found that acute mastitis had the highest rate (66.66%), and according to the symptomatology, there were found 2 cases (33.33%) with haemorrhagic mastitis, catarrhal mastitis and purulent mastitis. The highest incidence of clinical mastitis based on the number of lactations was in cows at 3 th and 4 th lactation (83.33%). Depending on age, all cases of clinical mastitis have been detected in cows aged 4-7 years. After the application of the Kerba Test method, a total of 10 cows (8.93%) were diagnosed with subclinical mastitis. The incidence of subclinical mastitis according to the age of the animals shows that 60% of the cases were recorded in cows aged 5-7 years. The incidence of subclinical mastitis regarding the number of lactations was 20% in cows at 5 th lactation, 10% in those at 4 th lactation, 30% in those at the 3 th lactation, 30% in the 2 nd lactation and 10% in the first lactation cows. Bacteriological exam revealed that the most commonly encountered pathogen was Staphylococcus spp. As regards the treatment of clinical mastitis, comparing the average of administrations, we observed a better efficacy of Masti Veyxym and Synulox LC therapy than in the treatment that used exclusively Synulox LC (2.5 vs. 4.5), although performed under the same conditions. In subclinical mastitis we achieved 100% healing after a single administration.
The silver content of the skin regeneration ointments can influence its regeneration process but ... more The silver content of the skin regeneration ointments can influence its regeneration process but in the meantime, it can take the benefit of the antibacterial properties of silver by avoiding the bacterial infection of an open wound. In the current study, the skin healing and regeneration capacity of bioactive glass with spherical gold nanocages (BGAuIND) in the Vaseline ointments were evaluated in vivo comparing the bioactive glass (BG)-Vaseline and bioactive glass with spherical gold (BGAuSP)-Vaseline ointments. Spherical gold nanocages are stabilized with silver and as a consequence the BGAuIND exhibits great antibacterial activity. Histological examination of the cutaneous tissue performed on day 8 indicates a more advanced regeneration process in rats treated with BGAuSP-Vaseline. The histopathological examination also confirms the results obtained after 11 days post-intervention, when the skin is completely regenerated at rats treated with BGAuSP-Vaseline compared with the oth...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2017
The aim of this study was to evaluate the effect of Regumate® product in association with other h... more The aim of this study was to evaluate the effect of Regumate® product in association with other hormonal products as a method for oestrus synchronization in gilts. The study was carried out between October 2016 – March 2017 in a private unit in Cluj County, and involved 16 gilts, 8 months of age. The synthetic progesterone Regumate was fed daily at 20 mg Altrenogest/gilt for 18 days. For all animals, 24 hours after cessation of progesterone treatment a PMSG hormone (Folligon) was administered (500 IU/gilt). After a rest period (48 hours) gilts received one injections of hCG hormone (Chorulon), 500 IU/gilt. Gilts were checked for oestrus twice daily and were artificially inseminated. Oestrus occurred on average 5 days after completion of treatment. All gilts expressed signs of oestrus between 24 and 30 hours with a mean value of 28.37 hours. Pregnancy rate was 75% after second insemination. The mean value of farrowed piglets was 10.16 and the average number of weaned piglets in was 9...
The structure of the salivary glands is different depending on the species and diet. The glandula... more The structure of the salivary glands is different depending on the species and diet. The glandular secretion can be serous, mucous or mixed. Within the same order, for instance in rodents, there are dissimilarities between the major salivary glands, even if the diet is similar. In this study, we used five Wistar rats, originating from the University of Medicine and Pharmacy “Iuliu Hațieganu” biobase, in Cluj-Napoca. After inducing neuroleptanalgesia, the method of euthanasia was cervical dislocation and then a stratigraphic dissection was accomplished. We mention that these euthanasia methods are allowed by 2010/63/UE directive of the European Parliament and the Council from September 22 of 2010, regarding animal protection used for scientific purposes. The external features were assessed and compared to published literature on other similar species. The macroscopic examination revealed that the major salivary glands in rats are similar to those from other species of mammals when re...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2014
The diameter of the ram testis is affected by seasonality and is associated with testosterone con... more The diameter of the ram testis is affected by seasonality and is associated with testosterone concentrations, presented a high correlation with the volume and sperm concentration. Selection for fertility in sheep can be accomplished through selecting for correlated traits in young rams such as age, testes size, testes length etc. The study was carried out to evaluate some parameters (testis length, testis width, testicular circumference, live weight) related with ram fertility and the qualities of ram semen in different age groups. The study was conducted in a farm located in Cluj County, on 20 rams of the breed Turcana Alba, grouped according to age into 4 batches. Weekly an ejaculate and the mentioned variables were measured. Semen samples were collected from each animal using an electro-ejaculator. There is a direct relationship between animals age and testicular circumference, testis length, testis width. With increase in age, ram showed increase percentages of motility and viab...
Summary The purpose of the paper was to improve the system of sheep oocytes assessment used for I... more Summary The purpose of the paper was to improve the system of sheep oocytes assessment used for IVM protocol. The research was carried out on a total of 43 oocytes collected by follicle aspiration method. The morphological evaluation of the oocytes was performed using an inverted Nikon microscope. Morphometric analysis was performed with the Motic Image Plus software, taking into study oocyte diameter, cumulus dimension, thickness of the pellucida zone. According to the morphological characteristics collected oocytes were included in two quality categories: cultivable (69.77%) and noncultivable oocytes (30.23%). Morphometric analysis led to the classification of oocytes in four quality classes: class I (44.19% ), class II (18.60%), class III (16.28%), class IV (20.93%). Results obtained at morphological and morphometric examination allowed us to develop a more accurate and objective classification of sheep oocytes, which will lead to their rigorous selection for in vitro maturation ...
The purpose of this paper was to improve the reproductive performances of sows after weaning in a... more The purpose of this paper was to improve the reproductive performances of sows after weaning in a private swine farm. The main objective was to implement modern reproductive biotechnologies (estrus synchronization, artificial insemination and early pregnancy diagnosis) in order to increase the economic efficiency of the reproductive sector of this farm. The biologic material used for the research was represented by 300 sows whose estrus was synchronized using three hormonal procedures (Regu-Mate administered collectively, Regu-Mate administered individually and PG600) as well as naturally, using stimulating boars. The results showed that the best methods of estrus induction and synchronization in sows use either Regu-Mate administered individually in fodder or PG600.
Biomaterials based on bioactive glass with gold nanoparticle composites have many applications in... more Biomaterials based on bioactive glass with gold nanoparticle composites have many applications in tissue engineering due to their tissue regeneration and angiogenesis capacities. The objectives of the study were to develop new composites using bioactive glass with gold nanospheres (BGAuSP) and gold nanocages (BGAuIND), individually introduced in alginate-pullulan (Alg-Pll) polymer, to evaluate their biocompatibility potential, and to compare the obtained results with those achieved when β-tricalcium phosphate-hydroxyapatite (βTCP/HA) replaced the BG. The novel composites underwent structural and morphological characterization followed by in vitro viability testing on fibroblast and osteoblast cell lines. Additionally, the biomaterials were subcutaneously implanted in Sprague Dawley rats, for in vivo biocompatibility assessment during 3 separate time frames (14, 30 and 60 days). The biological effects were evaluated by histopathology and immunohistochemistry. The physical characterization revealed the cross-linking between polymers and glasses/ceramics and demonstrated a suitable thermal stability for sterilization processes. The in vitro assays demonstrated adequate form, pore size of composites ranging from few micrometers up to 100 μm, while the self-assembled apatite layer formed after simulated body fluid immersion confirmed the composites' bioactivity. Viability assays have highlighted optimal cellular proliferation and in vitro biocompatibility for all tested composites. Furthermore, based on the in vivo subcutaneous analyses the polymer composites with BGAuNP have shown excellent biocompatibility at 14, 30 and 60 days, exhibiting marked angiogenesis while, tissue proliferation was confirmed by high number of Vimentin positive cells, in comparison with the polymer composite that contains βTCP/HA, which induced an inflammatory response represented by a foreign body reaction. The obtained results suggest promising, innovative, and biocompatible composites with bioactive properties for future soft tissue and bone engineering endeavours.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2020
The purpose of this research was to establish the incidence and to compare the efficiency of two ... more The purpose of this research was to establish the incidence and to compare the efficiency of two products used for the hormonal stimulation of ovarian hypotrophy. Gynaecological investigations were performed in 2 farms. The animals with ovarian hypotrophy (ovaries smaller than normal and with a slippery surface) were divided in 2 equal groups depending on the protocol applied: Progesterone (Prid Delta, CEVA) and Progesterone (Dehydroxyprogesterone 10%, Faculty of Veterinary Medicine Cluj-Napoca). In case of group 1 the treatment was performed with Prid Delta (CEVA), vaginal use, 1.55 g of progesterone/animal for 7 days. For the animals of group 2 the treatment was performed with Dehydroxyprogesterone, subcutaneous injection, 1.5 g of dehydroxyprogesterone/animal for 7 days. For each group were observed: the estrous signals, the interval of estrous apparition, the number of gestation obtained after the first artificial insemination.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2019
The purpose of this research was to conduct an ultrasound exam in the interval between 49-120 day... more The purpose of this research was to conduct an ultrasound exam in the interval between 49-120 days of gestation for the determination of fetus sex and to establish the interval when the fetal sexing is possible. The research was carried out in three farms from Transylvania. In farm A were examined 25 animals, in farm B 13 animals and in farm C 11 animals. The diagnosis of the fetal sexing was possible for 35 cases, 14 animals were diagnosed as female and 21 were male. In the interval between 56-65 days of gestation the diagnosis of fetal sexing was established by viewing the genital tubercle and in the interval between 65-90 days of gestation the diagnosis was established by viewing the secondary genital organs. In 14 cases the diagnosis was not set; in 4 cases the conception product has not been sufficiently developed, the genital tubercle was not visible, and in 10 cases the fetus was too big and was impossible to localize the genital organs.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2019
Blood pressure is a cardinal vital sign that gives important information about the cardiovascular... more Blood pressure is a cardinal vital sign that gives important information about the cardiovascular function and about hemodynamic trends during anaesthesia, in critical ill patients and during experimental procedures (Rehman and Nelson, 2018). Arterial blood pressure can be evaluated by direct technique (arterial catheter) or indirect technique (Doppler or oscillometry). Direct measurement is gold standard for blood pressure measurement, giving accurate beat-to-beat information and also allow collection of blood samples. However, it is more invasive and requires equipment for monitoring and experience to place the arterial catheter (Araghi et al., 2006; Ward and Langton, 2007; Wingfield and Raffe, 2002). Given its importance in directing care, it is essential to measure blood pressure accurately and consistently.The aim of this article is to present all technique found in literature, with its advantages and disadvantages, with the errors that may occur so that the researchers can hav...
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2019
In the present study, scaffolds based on alginate-pullulan-bioactive glass-ceramic with 0.5 and 1... more In the present study, scaffolds based on alginate-pullulan-bioactive glass-ceramic with 0.5 and 1.5 mol % copper oxide were orthotopically implanted in experimental rat models to assess their ability to heal an induced bone defect. By implying magnetic resonance and imaging scans together with histological evaluation of the processed samples, a progressive healing of bone was observed within 5 weeks. Furthermore, as the regenerative process continued, new bone tissue was formed, enhancing the growth of irregular bone spicules around the scaffolds. A significantly higher amount of new bone was formed (37%) in the defect that received the composite with 1.5 mol % CuO (in glass-ceramic matrix) content implant. Nevertheless, the bone regeneration obtained by scaffold with 0.5 mol % CuO implanted is comparable with the alginatepullulan-β-tricalcium phosphate/hydroxiapatite composite implant. The assessed amount of new bone formed was found to be between 29.75 and 37.15% for all the composition involved in the present study. During this process a regeneration process was shown when the alginate-pullulan composite materials were involved, fact that indicate the great potential of these materials to be used in tissue engineering.
The aim of the study was to identify if there is a connection between the time of administration ... more The aim of the study was to identify if there is a connection between the time of administration of alpha-blocker medication and cataract surgery complications. Furthermore, it was explored whether discontinuation of tamsulosin before cataract surgery influences the manifestations of intraoperative floppy iris syndrome in rats. An experimental study was conducted on 20 male Wistar rats aged 1.5-2 years (body mean weight 357 g), which were divided into four equal groups: Group 1, under tamsulosin hydrochloride 0.4 mg/day for 2 months; group 2, without any treatment in the first month, followed by tamsulosin; group 3, under tamsulosin for 1 month, followed by 1 month without any treatment; and group 4, control. The pupillary diameter was assessed before instillation of 0.5% tropicamide (mydriatic and cycloplegic agent), after 1 or 2 h from instillation and postoperatively. Furthermore, pupil constriction during surgery, the presence of floppy iris and prolapse of the iris following the main incision and during serum injection were also assessed. Other analyzed features included iris rupture, posterior capsule tear, vitreous loss, lens fragments in the vitreous, suprachoroidal bleeding and corneal haze. The iris dilator muscle thickness and the posterior epithelium was measured using light microscopy. In the control group, the largest pupillary diameters were indicated and in group 1, the smallest pupillary diameters were revealed. Statistically significant differences were indicated between group 1 and 2 and group 3 and 4. Floppy iris, iris prolapse in the main incision and during serum injection appeared at maximum frequency for group 1 with continuous treatment and low frequency for the control group. Lens fragments in the vitreous were present in the groups 1-3. Notably, intraoperative miosis had statistical relevance in group 1 and group 2. To conclude, the present findings suggested there is a clear connection between the time of administration of the alpha-blocker medication and most cataract complications. The results indicated that discontinuation of tamsulosin for one month largely reduces the manifestations of intraoperative floppy iris syndrome in rats, which may suggest to change preoperative treatment for benign prostatic hypertrophy with another class of drugs that do not interact with the iris receptors.
Journal of tissue engineering and regenerative medicine, 2018
Composites based on sodium alginate, pullulan, and bioactive SiO -CaO-P O glass-ceramics with cop... more Composites based on sodium alginate, pullulan, and bioactive SiO -CaO-P O glass-ceramics with copper oxide were prepared as capsules. The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy. The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance. The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained. Different crystalline shapes of hydroxyapatite were formed after in vitro and in ...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2017
The aim of this study was to evaluate the effect of Altresyn® product (progesterone-based produc... more The aim of this study was to evaluate the effect of Altresyn® product (progesterone-based product) as a method for synchronization of oestrus in gilts. The gilts were divided into three experimental groups. The synthetic progesterone Altrenogest was fed daily to 39 gilts at 20 mg/gilts for 18 days. In case of the first group (n = 11 gilts) the treatment was performed using only Altresyn product. In group II (n=13 gilts) was administered the prostaglandin hormone (Prosolvin), 0.75 mg at 24 hours after Altrenogest treatment suppression. Group III (n=15 gilts) received one injections of PMSG hormone (Folligon), 500 IU/animal. Gilts were checked for oestrus twice daily and were artificially inseminated. All gilts expressed signs of oestrus between 3 and 6 days after withdrawal of Altrenogest. At the groups that were administered prostaglandin and PMSG there was a shorter duration of oestrus (group III - 26.66 hours, group II - 27.07 hours) compared to group I (28.54 hours). In this s...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2017
Reproductive efficiency is an essential requirement for the profitability of swine farms and the ... more Reproductive efficiency is an essential requirement for the profitability of swine farms and the quality of fodder can have a negative impact on this aspect. We carried out investigations regarding the influence of fodder quality on reproductive efficiency and the histological structure of the ovary and oviduct in 45 sows from a farm in Bihor county. We observed that corn utilized in feeding the sows was stored in inadequate conditions which allowed the development of moulds. The combined fodder, resulted after grinding the cereals, was stored directly on the concrete floor in inappropriate areas, concerning the hygiene. The animals were given green fodder directly on the stall’s floor, which favours mould expansion. Upon histological examination, the ovary presented an exaggerated activity and there was a marked congestion in the oviduct, with a tendency of the epithelium toward pseudostratification. The precarious conditions of cereal and combined fodder storing and administrati...
Infertility in cows is one of the major problems with great economic implications. The purpose of... more Infertility in cows is one of the major problems with great economic implications. The purpose of this study was to identify by global and individual gynecological investigation major ovarian diseases and to determinate their incidence in the relation with hot season at Aberdeen Angus cows. The study was carried out in June 2019 in private unit in Timis county, Romania, on a number of 93 Aberdeen Angus cows in order to determine the prevalence of different ovarian diseases at this breed in a summer season. Gynecological investigations were performed in order to establish the prevalence of ovarian diseases in the summer season. The results showed different pathological entity variation. Thus, 50.22% of the animals taken into study presented ovarian hypofunction, 27.9 % presented corpus luteum , 0.93% polycystic ovary with uterine adherences and 7.44% ovarian follicle
”Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, Iași, 2019
The objective of this study was to evaluate the effects of prostaglandins (natural and synthetic ... more The objective of this study was to evaluate the effects of prostaglandins (natural and synthetic analogues) on estrous synchronization in beef cows. The study was carried out between May-August 2019 in a private unit in Timis County, and involved 54 Aberdeen Angus cows diagnosed with ovarian luteal tissue: corpus luteum (n=36 cows), cystic corpus luteum (n= 8 cows) and luteal cysts (n=10 cows). Two products were used for the treatment of this pathology: PGF Veyx Forte (n=27 cows) and Enzaprost (n=27 cows). The interval between hormonal treatment and the onset of estrous was ranging from 48-96 hours. In this trial 83.33% of the animals expressed signs of estrous. In the present experiment the conception rate after the artificial insemination was 71.11%.
Lucrări Științifice - Medicină Veterinară, Universitatea de Științe Agricole și Medicină Veterinară "Ion Ionescu de la Brad" Iași, 2019
The purpose of this research was to evaluate an intrauterine suspension in the treatment of retai... more The purpose of this research was to evaluate an intrauterine suspension in the treatment of retained placenta. A therapeutic option that affects, as little as possible the female reproductive life, thus reducing the associated economic implication is the ideal in the treatment of retained placenta. In this study, we analyzed the cases of retained placenta in a farm from Cluj county, the incidence, the diagnosis and the treatment of this pathology in cows. The study was carried out during January 2017-April 2019 and included 216 animals that have calved in that period. Of these, 16 animals were with retained placenta at 12-15 hours after calving, which represents 7.40%. After the treatment, the service period, the number of doses of semen needed for one gestation and the costs of treatment were established.
Lucrări Științifice - Medicină Veterinară, Universitatea de Științe Agricole și Medicină Veterinară "Ion Ionescu de la Brad" Iași, 2017
The purpose of this work was to supervise the activity and health of the mammary gland in dairy c... more The purpose of this work was to supervise the activity and health of the mammary gland in dairy cows in a dairy specialty unit in Cluj County. The objectives of this study can be summarized as follows: diagnosis of mastitis, carrying out a study on the incidence of mastitis according to some factors, performing the microbiological exam and treatment. Following the clinical examination, 6 cows (5.36%) were diagnosed with clinical mastitis. Depending on the type of lesion, it was found that acute mastitis had the highest rate (66.66%), and according to the symptomatology, there were found 2 cases (33.33%) with haemorrhagic mastitis, catarrhal mastitis and purulent mastitis. The highest incidence of clinical mastitis based on the number of lactations was in cows at 3 th and 4 th lactation (83.33%). Depending on age, all cases of clinical mastitis have been detected in cows aged 4-7 years. After the application of the Kerba Test method, a total of 10 cows (8.93%) were diagnosed with subclinical mastitis. The incidence of subclinical mastitis according to the age of the animals shows that 60% of the cases were recorded in cows aged 5-7 years. The incidence of subclinical mastitis regarding the number of lactations was 20% in cows at 5 th lactation, 10% in those at 4 th lactation, 30% in those at the 3 th lactation, 30% in the 2 nd lactation and 10% in the first lactation cows. Bacteriological exam revealed that the most commonly encountered pathogen was Staphylococcus spp. As regards the treatment of clinical mastitis, comparing the average of administrations, we observed a better efficacy of Masti Veyxym and Synulox LC therapy than in the treatment that used exclusively Synulox LC (2.5 vs. 4.5), although performed under the same conditions. In subclinical mastitis we achieved 100% healing after a single administration.
The silver content of the skin regeneration ointments can influence its regeneration process but ... more The silver content of the skin regeneration ointments can influence its regeneration process but in the meantime, it can take the benefit of the antibacterial properties of silver by avoiding the bacterial infection of an open wound. In the current study, the skin healing and regeneration capacity of bioactive glass with spherical gold nanocages (BGAuIND) in the Vaseline ointments were evaluated in vivo comparing the bioactive glass (BG)-Vaseline and bioactive glass with spherical gold (BGAuSP)-Vaseline ointments. Spherical gold nanocages are stabilized with silver and as a consequence the BGAuIND exhibits great antibacterial activity. Histological examination of the cutaneous tissue performed on day 8 indicates a more advanced regeneration process in rats treated with BGAuSP-Vaseline. The histopathological examination also confirms the results obtained after 11 days post-intervention, when the skin is completely regenerated at rats treated with BGAuSP-Vaseline compared with the oth...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture, 2017
The aim of this study was to evaluate the effect of Regumate® product in association with other h... more The aim of this study was to evaluate the effect of Regumate® product in association with other hormonal products as a method for oestrus synchronization in gilts. The study was carried out between October 2016 – March 2017 in a private unit in Cluj County, and involved 16 gilts, 8 months of age. The synthetic progesterone Regumate was fed daily at 20 mg Altrenogest/gilt for 18 days. For all animals, 24 hours after cessation of progesterone treatment a PMSG hormone (Folligon) was administered (500 IU/gilt). After a rest period (48 hours) gilts received one injections of hCG hormone (Chorulon), 500 IU/gilt. Gilts were checked for oestrus twice daily and were artificially inseminated. Oestrus occurred on average 5 days after completion of treatment. All gilts expressed signs of oestrus between 24 and 30 hours with a mean value of 28.37 hours. Pregnancy rate was 75% after second insemination. The mean value of farrowed piglets was 10.16 and the average number of weaned piglets in was 9...
The structure of the salivary glands is different depending on the species and diet. The glandula... more The structure of the salivary glands is different depending on the species and diet. The glandular secretion can be serous, mucous or mixed. Within the same order, for instance in rodents, there are dissimilarities between the major salivary glands, even if the diet is similar. In this study, we used five Wistar rats, originating from the University of Medicine and Pharmacy “Iuliu Hațieganu” biobase, in Cluj-Napoca. After inducing neuroleptanalgesia, the method of euthanasia was cervical dislocation and then a stratigraphic dissection was accomplished. We mention that these euthanasia methods are allowed by 2010/63/UE directive of the European Parliament and the Council from September 22 of 2010, regarding animal protection used for scientific purposes. The external features were assessed and compared to published literature on other similar species. The macroscopic examination revealed that the major salivary glands in rats are similar to those from other species of mammals when re...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2014
The diameter of the ram testis is affected by seasonality and is associated with testosterone con... more The diameter of the ram testis is affected by seasonality and is associated with testosterone concentrations, presented a high correlation with the volume and sperm concentration. Selection for fertility in sheep can be accomplished through selecting for correlated traits in young rams such as age, testes size, testes length etc. The study was carried out to evaluate some parameters (testis length, testis width, testicular circumference, live weight) related with ram fertility and the qualities of ram semen in different age groups. The study was conducted in a farm located in Cluj County, on 20 rams of the breed Turcana Alba, grouped according to age into 4 batches. Weekly an ejaculate and the mentioned variables were measured. Semen samples were collected from each animal using an electro-ejaculator. There is a direct relationship between animals age and testicular circumference, testis length, testis width. With increase in age, ram showed increase percentages of motility and viab...
Summary The purpose of the paper was to improve the system of sheep oocytes assessment used for I... more Summary The purpose of the paper was to improve the system of sheep oocytes assessment used for IVM protocol. The research was carried out on a total of 43 oocytes collected by follicle aspiration method. The morphological evaluation of the oocytes was performed using an inverted Nikon microscope. Morphometric analysis was performed with the Motic Image Plus software, taking into study oocyte diameter, cumulus dimension, thickness of the pellucida zone. According to the morphological characteristics collected oocytes were included in two quality categories: cultivable (69.77%) and noncultivable oocytes (30.23%). Morphometric analysis led to the classification of oocytes in four quality classes: class I (44.19% ), class II (18.60%), class III (16.28%), class IV (20.93%). Results obtained at morphological and morphometric examination allowed us to develop a more accurate and objective classification of sheep oocytes, which will lead to their rigorous selection for in vitro maturation ...
The purpose of this paper was to improve the reproductive performances of sows after weaning in a... more The purpose of this paper was to improve the reproductive performances of sows after weaning in a private swine farm. The main objective was to implement modern reproductive biotechnologies (estrus synchronization, artificial insemination and early pregnancy diagnosis) in order to increase the economic efficiency of the reproductive sector of this farm. The biologic material used for the research was represented by 300 sows whose estrus was synchronized using three hormonal procedures (Regu-Mate administered collectively, Regu-Mate administered individually and PG600) as well as naturally, using stimulating boars. The results showed that the best methods of estrus induction and synchronization in sows use either Regu-Mate administered individually in fodder or PG600.
Biomaterials based on bioactive glass with gold nanoparticle composites have many applications in... more Biomaterials based on bioactive glass with gold nanoparticle composites have many applications in tissue engineering due to their tissue regeneration and angiogenesis capacities. The objectives of the study were to develop new composites using bioactive glass with gold nanospheres (BGAuSP) and gold nanocages (BGAuIND), individually introduced in alginate-pullulan (Alg-Pll) polymer, to evaluate their biocompatibility potential, and to compare the obtained results with those achieved when β-tricalcium phosphate-hydroxyapatite (βTCP/HA) replaced the BG. The novel composites underwent structural and morphological characterization followed by in vitro viability testing on fibroblast and osteoblast cell lines. Additionally, the biomaterials were subcutaneously implanted in Sprague Dawley rats, for in vivo biocompatibility assessment during 3 separate time frames (14, 30 and 60 days). The biological effects were evaluated by histopathology and immunohistochemistry. The physical characterization revealed the cross-linking between polymers and glasses/ceramics and demonstrated a suitable thermal stability for sterilization processes. The in vitro assays demonstrated adequate form, pore size of composites ranging from few micrometers up to 100 μm, while the self-assembled apatite layer formed after simulated body fluid immersion confirmed the composites' bioactivity. Viability assays have highlighted optimal cellular proliferation and in vitro biocompatibility for all tested composites. Furthermore, based on the in vivo subcutaneous analyses the polymer composites with BGAuNP have shown excellent biocompatibility at 14, 30 and 60 days, exhibiting marked angiogenesis while, tissue proliferation was confirmed by high number of Vimentin positive cells, in comparison with the polymer composite that contains βTCP/HA, which induced an inflammatory response represented by a foreign body reaction. The obtained results suggest promising, innovative, and biocompatible composites with bioactive properties for future soft tissue and bone engineering endeavours.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2020
The purpose of this research was to establish the incidence and to compare the efficiency of two ... more The purpose of this research was to establish the incidence and to compare the efficiency of two products used for the hormonal stimulation of ovarian hypotrophy. Gynaecological investigations were performed in 2 farms. The animals with ovarian hypotrophy (ovaries smaller than normal and with a slippery surface) were divided in 2 equal groups depending on the protocol applied: Progesterone (Prid Delta, CEVA) and Progesterone (Dehydroxyprogesterone 10%, Faculty of Veterinary Medicine Cluj-Napoca). In case of group 1 the treatment was performed with Prid Delta (CEVA), vaginal use, 1.55 g of progesterone/animal for 7 days. For the animals of group 2 the treatment was performed with Dehydroxyprogesterone, subcutaneous injection, 1.5 g of dehydroxyprogesterone/animal for 7 days. For each group were observed: the estrous signals, the interval of estrous apparition, the number of gestation obtained after the first artificial insemination.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2019
The purpose of this research was to conduct an ultrasound exam in the interval between 49-120 day... more The purpose of this research was to conduct an ultrasound exam in the interval between 49-120 days of gestation for the determination of fetus sex and to establish the interval when the fetal sexing is possible. The research was carried out in three farms from Transylvania. In farm A were examined 25 animals, in farm B 13 animals and in farm C 11 animals. The diagnosis of the fetal sexing was possible for 35 cases, 14 animals were diagnosed as female and 21 were male. In the interval between 56-65 days of gestation the diagnosis of fetal sexing was established by viewing the genital tubercle and in the interval between 65-90 days of gestation the diagnosis was established by viewing the secondary genital organs. In 14 cases the diagnosis was not set; in 4 cases the conception product has not been sufficiently developed, the genital tubercle was not visible, and in 10 cases the fetus was too big and was impossible to localize the genital organs.
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2019
Blood pressure is a cardinal vital sign that gives important information about the cardiovascular... more Blood pressure is a cardinal vital sign that gives important information about the cardiovascular function and about hemodynamic trends during anaesthesia, in critical ill patients and during experimental procedures (Rehman and Nelson, 2018). Arterial blood pressure can be evaluated by direct technique (arterial catheter) or indirect technique (Doppler or oscillometry). Direct measurement is gold standard for blood pressure measurement, giving accurate beat-to-beat information and also allow collection of blood samples. However, it is more invasive and requires equipment for monitoring and experience to place the arterial catheter (Araghi et al., 2006; Ward and Langton, 2007; Wingfield and Raffe, 2002). Given its importance in directing care, it is essential to measure blood pressure accurately and consistently.The aim of this article is to present all technique found in literature, with its advantages and disadvantages, with the errors that may occur so that the researchers can hav...
Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2019
In the present study, scaffolds based on alginate-pullulan-bioactive glass-ceramic with 0.5 and 1... more In the present study, scaffolds based on alginate-pullulan-bioactive glass-ceramic with 0.5 and 1.5 mol % copper oxide were orthotopically implanted in experimental rat models to assess their ability to heal an induced bone defect. By implying magnetic resonance and imaging scans together with histological evaluation of the processed samples, a progressive healing of bone was observed within 5 weeks. Furthermore, as the regenerative process continued, new bone tissue was formed, enhancing the growth of irregular bone spicules around the scaffolds. A significantly higher amount of new bone was formed (37%) in the defect that received the composite with 1.5 mol % CuO (in glass-ceramic matrix) content implant. Nevertheless, the bone regeneration obtained by scaffold with 0.5 mol % CuO implanted is comparable with the alginatepullulan-β-tricalcium phosphate/hydroxiapatite composite implant. The assessed amount of new bone formed was found to be between 29.75 and 37.15% for all the composition involved in the present study. During this process a regeneration process was shown when the alginate-pullulan composite materials were involved, fact that indicate the great potential of these materials to be used in tissue engineering.
The aim of the study was to identify if there is a connection between the time of administration ... more The aim of the study was to identify if there is a connection between the time of administration of alpha-blocker medication and cataract surgery complications. Furthermore, it was explored whether discontinuation of tamsulosin before cataract surgery influences the manifestations of intraoperative floppy iris syndrome in rats. An experimental study was conducted on 20 male Wistar rats aged 1.5-2 years (body mean weight 357 g), which were divided into four equal groups: Group 1, under tamsulosin hydrochloride 0.4 mg/day for 2 months; group 2, without any treatment in the first month, followed by tamsulosin; group 3, under tamsulosin for 1 month, followed by 1 month without any treatment; and group 4, control. The pupillary diameter was assessed before instillation of 0.5% tropicamide (mydriatic and cycloplegic agent), after 1 or 2 h from instillation and postoperatively. Furthermore, pupil constriction during surgery, the presence of floppy iris and prolapse of the iris following the main incision and during serum injection were also assessed. Other analyzed features included iris rupture, posterior capsule tear, vitreous loss, lens fragments in the vitreous, suprachoroidal bleeding and corneal haze. The iris dilator muscle thickness and the posterior epithelium was measured using light microscopy. In the control group, the largest pupillary diameters were indicated and in group 1, the smallest pupillary diameters were revealed. Statistically significant differences were indicated between group 1 and 2 and group 3 and 4. Floppy iris, iris prolapse in the main incision and during serum injection appeared at maximum frequency for group 1 with continuous treatment and low frequency for the control group. Lens fragments in the vitreous were present in the groups 1-3. Notably, intraoperative miosis had statistical relevance in group 1 and group 2. To conclude, the present findings suggested there is a clear connection between the time of administration of the alpha-blocker medication and most cataract complications. The results indicated that discontinuation of tamsulosin for one month largely reduces the manifestations of intraoperative floppy iris syndrome in rats, which may suggest to change preoperative treatment for benign prostatic hypertrophy with another class of drugs that do not interact with the iris receptors.
Journal of tissue engineering and regenerative medicine, 2018
Composites based on sodium alginate, pullulan, and bioactive SiO -CaO-P O glass-ceramics with cop... more Composites based on sodium alginate, pullulan, and bioactive SiO -CaO-P O glass-ceramics with copper oxide were prepared as capsules. The obtained samples were structurally characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), and their bioactivity and biocompatibility properties were also tested both in vitro and in vivo by XRD, FT-IR, SEM, and high-resolution transmission electron microscopy. The fibroblast and osteoblast cell viability assays have shown good proliferation rates for all investigated samples, whereas all composites exhibited a good in vivo tolerance. The recovered composites after 5 weeks' in vivo and in vitro trials evidenced clear macroscopic alterations; particularly, after soaking in simulated body fluid, they have a corn flake aspect, and after their in vivo inoculation, a globular shape is retained. Different crystalline shapes of hydroxyapatite were formed after in vitro and in ...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2017
The aim of this study was to evaluate the effect of Altresyn® product (progesterone-based produc... more The aim of this study was to evaluate the effect of Altresyn® product (progesterone-based product) as a method for synchronization of oestrus in gilts. The gilts were divided into three experimental groups. The synthetic progesterone Altrenogest was fed daily to 39 gilts at 20 mg/gilts for 18 days. In case of the first group (n = 11 gilts) the treatment was performed using only Altresyn product. In group II (n=13 gilts) was administered the prostaglandin hormone (Prosolvin), 0.75 mg at 24 hours after Altrenogest treatment suppression. Group III (n=15 gilts) received one injections of PMSG hormone (Folligon), 500 IU/animal. Gilts were checked for oestrus twice daily and were artificially inseminated. All gilts expressed signs of oestrus between 3 and 6 days after withdrawal of Altrenogest. At the groups that were administered prostaglandin and PMSG there was a shorter duration of oestrus (group III - 26.66 hours, group II - 27.07 hours) compared to group I (28.54 hours). In this s...
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2017
Reproductive efficiency is an essential requirement for the profitability of swine farms and the ... more Reproductive efficiency is an essential requirement for the profitability of swine farms and the quality of fodder can have a negative impact on this aspect. We carried out investigations regarding the influence of fodder quality on reproductive efficiency and the histological structure of the ovary and oviduct in 45 sows from a farm in Bihor county. We observed that corn utilized in feeding the sows was stored in inadequate conditions which allowed the development of moulds. The combined fodder, resulted after grinding the cereals, was stored directly on the concrete floor in inappropriate areas, concerning the hygiene. The animals were given green fodder directly on the stall’s floor, which favours mould expansion. Upon histological examination, the ovary presented an exaggerated activity and there was a marked congestion in the oviduct, with a tendency of the epithelium toward pseudostratification. The precarious conditions of cereal and combined fodder storing and administrati...
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