Journal of Veterinary Medical Researches (Print), Dec 12, 2017
Electron tomography is a new technique for obtaining detailed 3D structures of both biological an... more Electron tomography is a new technique for obtaining detailed 3D structures of both biological and non-biological samples relevant to the life and biomedical sciences. Electron tomography is an extension of the traditional transmission electron microscopy. Different protocols concerning the 3D electron tomography, Conventional TEM, Immuno-electron microscopy and cryoEM will be covered. With these methods, relatively large volumes of resinembedded biological structures can be analyzed at resolutions of a few nm within a reasonable expenditure of time. Therefore, this work could support the collaborative research activities of many investigators from various departments in the faculty of veterinary medicine whose work has already had a major impact in the areas of biological, life and physical sciences.
We describe the morphological, histological and histochemical characteristics of the uropygial gl... more We describe the morphological, histological and histochemical characteristics of the uropygial gland of the duck (Anas platyrhynchos) and pigeon (Columba livia). The weight and dimensions of the glands were recorded. The gland openings onto the papilla in ducks were surrounded by double tufts of downy feathers; the tufts were held together by an oily secretion. In the pigeon, the gland opening was onto naked skin. Samples for light and transmission electron microscopy were obtained from adult birds. Gland morphometry of the duck showed greater dimensions and relative gland weight than the pigeon. In both species the gland was lined by stratified epithelium, packed with secretary tubules and filled with oil droplets that were discharged into a central cavity. In the pigeon, the gland was branched and alveolar with a wide central lumen, whereas in the duck it was simple and branched tubular. Ultrastructural analysis of both species revealed that the fat globules tended to condense in the peripheral layers of the secretory and degenerative cells, and the nuclei exhibited pyknosis, which suggest the holocrine nature of the gland. Morphometric and histological analysis of the gland showed that the architecture is similar among birds; however, some species-specific differences suggest a functional correlation with the habitat.
Journal of Advanced Veterinary and Animal Research, 2015
The present study was aimed to investigate the morphology and histomorphometry of stomach and gas... more The present study was aimed to investigate the morphology and histomorphometry of stomach and gastric mucosa in western grey kangaroo (Macropus fuliginosus). The stomach was composed of three indistinctive separate parts namely sacciform forestomach, tubiform forestomach, and hindstomach. The tubiform forestomach was the main tubular section of the organ. The stomach had a compound lining. The non-glandular mucosa occupied the medial blind sac (MBS) of the sacciform forestomach; the layer covered about one-third of the tubiform forestomach (non-glandular region) and the entire length of the gastric sulcus. The glandular part lined the parietal blind sac (PBS) of sacciform forestomach and the cardiac gland region of tubiform forestomach as well as fundic and pyloric gland regions of the hindstomach. The cardiac mucosa had smooth and folded areas; these were filled with mixed glands. In the fundic glands, the parietal cells outnumbered the chief cells. The pyloric glands were of serous-like in characteristics. In conclusion, gross and histological structures of the stomach of western grey kangaroo are adaptive with its food habitat, which allows thorough mixing of highly fibrous grasses.
DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2014
The lyssa is a morphologically supporting structure situated along the median plan on the ventral... more The lyssa is a morphologically supporting structure situated along the median plan on the ventral surface of the apex of the tongue in some animals. The present study aimed to describe the morphological differences of the lyssa in carnivores (dog and cat) and camel using conformist macroscopic and microscopic methods as well as evaluation of its surgical resection in the dog and cat. The lyssa is a rod-shaped in dog, strip like structure in cat and ridge-like structure in camel. It showed straight course in dog and helical appearance in cat. In the studied species, the lyssa was formed from adipose tissue intermingled with irregular connective tissue. However, dense striated muscles fibers were seen in the caudal half of the lyssa in dog. Besides, few bundles of longitudinally running muscle fibers were observed in cat and camel. In dog, the lyssa was defined by a coarse sheath of connective tissue, this capsule was ill-defined in cat and absent in camel. In cat, a pyramidal extension of the adipose tissue of the lyssa was attached to intrinsic striated muscle of the tongue. Full function of the tongue could be achieved after surgical resection of lyssa in dogs and cats.
related peptide; Protein gene product 9.5. The fact that the splice variant form of choline acety... more related peptide; Protein gene product 9.5. The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in peripheral organs, including sensory ones, preferentially than the common type (cChAT) is well known. In the current study the possible functional significance of this variant in sensory neurons has been characterized immunohistochemically by investigating the pChAT-immunoreactivity (IR) in the trigeminal ganglia (TG) of the guinea pig. We documented an almost uniform distribution and a considerable number of pChAT-immunoreactivity of all trigeminal neurons. The size of pChAT-IR neurons varied from small to medium-size, although large-sized neurons also observed. Most pChAT reactivity was mainly in the cytoplasm with few number of pChAT-IR neurons had nuclear staining. Double immunofluorescent study showed that a great proportion of substance P (SP)- and calcitonin gene-related peptide (CGRP)-positive trigeminal cells showed pChAT-immunoreactivity, al...
We describe the morphological, histological and histochemical characteristics of the uropygial gl... more We describe the morphological, histological and histochemical characteristics of the uropygial gland of the duck (Anas platyrhynchos) and pigeon (Columba livia). The weight and dimensions of the glands were recorded. The gland openings onto the papilla in ducks were surrounded by double tufts of downy feathers; the tufts were held together by an oily secretion. In the pigeon, the gland opening was onto naked skin. Samples for light and transmission electron microscopy were obtained from adult birds. Gland morphometry of the duck showed greater dimensions and relative gland weight than the pigeon. In both species the gland was lined by stratified epithelium, packed with secretary tubules and filled with oil droplets that were discharged into a central cavity. In the pigeon, the gland was branched and alveolar with a wide central lumen, whereas in the duck it was simple and branched tubular. Ultrastructural analysis of both species revealed that the fat globules tended to condense in the peripheral layers of the secretory and degenerative cells, and the nuclei exhibited pyknosis, which suggest the holocrine nature of the gland. Morphometric and histological analysis of the gland showed that the architecture is similar among birds; however, some species-specific differences suggest a functional correlation with the habitat.
Journal of Animal and Veterinary Advances, Jun 23, 2014
The lyssa is a morphologically supporting structure situated along the median plan on the ventral... more The lyssa is a morphologically supporting structure situated along the median plan on the ventral surface of the apex of the tongue in some animals. The present study aimed to describe the morphological differences of the lyssa in carnivores (dog and cat) and camel using conformist macroscopic and microscopic methods as well as evaluation of its surgical resection in the dog and cat. The lyssa is a rod-shaped in dog, strip like structure in cat and ridge-like structure in camel. It showed straight course in dog and helical appearance in cat. In the studied species, the lyssa was formed from adipose tissue intermingled with irregular connective tissue. However, dense striated muscles fibers were seen in the caudal half of the lyssa in dog. Besides, few bundles of longitudinally running muscle fibers were observed in cat and camel. In dog, the lyssa was defined by a coarse sheath of connective tissue, this capsule was ill-defined in cat and absent in camel. In cat, a pyramidal extension of the adipose tissue of the lyssa was attached to intrinsic striated muscle of the tongue. Full function of the tongue could be achieved after surgical resection of lyssa in dogs and cats.
The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in periph... more The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in peripheral organs, including sensory ones, preferentially than the common type (cChAT) is well known. In the current study the possible functional significance of this variant in sensory neurons has been characterized immunohistochemically by investigating the pChAT-immunoreactivity (IR) in the trigeminal ganglia (TG) of the guinea pig. We documented an almost uniform distribution and a considerable number of pChATimmunoreactivity of all trigeminal neurons. The size of pChAT-IR neurons varied from small to medium-size, although large-sized neurons also observed. Most pChAT reactivity was mainly in the cytoplasm with few number of pChAT-IR neurons had nuclear staining. Double immunofluorescent study showed that a great proportion of substance P (SP)-and calcitonin gene-related peptide (CGRP)-positive trigeminal cells showed pChATimmunoreactivity, although those with SP outnumbered those with CGRP. The intracellular expression of pChAT (which differs from that of cChAT) probably reflecting a difference in the physiological roles between pChAT and cChAT in ACh production in distinct intracellular compartments. The present data suggest also that pChAT may play roles other than nociception and may be involved in the sensory functions of the TG neurons.
The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in periph... more The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in peripheral organs, including sensory ones, preferentially than the common type (cChAT) is well known. In the current study the possible functional significance of this variant in sensory neurons has been characterized immunohistochemically by investigating the pChAT-immunoreactivity (IR) in the trigeminal ganglia (TG) of the guinea pig. We documented an almost uniform distribution and a considerable number of pChATimmunoreactivity of all trigeminal neurons. The size of pChAT-IR neurons varied from small to medium-size, although large-sized neurons also observed. Most pChAT reactivity was mainly in the cytoplasm with few number of pChAT-IR neurons had nuclear staining. Double immunofluorescent study showed that a great proportion of substance P (SP)-and calcitonin gene-related peptide (CGRP)-positive trigeminal cells showed pChATimmunoreactivity, although those with SP outnumbered those with CGRP. The intracellular expression of pChAT (which differs from that of cChAT) probably reflecting a difference in the physiological roles between pChAT and cChAT in ACh production in distinct intracellular compartments. The present data suggest also that pChAT may play roles other than nociception and may be involved in the sensory functions of the TG neurons.
The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis... more The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal duct as well as the principal cells of the rabbit epididymis. In conclusion, the distribution of the S-100 proteins in the testis and epididymis might point out to its roles in the male reproduction.
Abstract: The present investigation was conducted to demonstrate S-100 protein in the testis and ... more Abstract: The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal...
The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis... more The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal duct as well as the principal cells of the rabbit epididymis. In conclusion, the distribution of the S-100 proteins in the testis and epididymis might point out to its roles in the male reproduction.
The present study was aimed to investigate the morphology and histomorphometry of stomach and gas... more The present study was aimed to investigate the morphology and histomorphometry of stomach and gastric mucosa in western grey kangaroo (Macropus fuliginosus). The stomach was composed of three indistinctive separate parts namely sacciform forestomach, tubiform forestomach, and hindstomach. The tubiform forestomach was the main tubular section of the organ. The stomach had a compound lining. The non-glandular mucosa occupied the medial blind sac (MBS) of the sacciform forestomach; the layer covered about one-third of the tubiform forestomach (non-glandular region) and the entire length of the gastric sulcus. The glandular part lined the parietal blind sac (PBS) of sacciform forestomach and the cardiac gland region of tubiform forestomach as well as fundic and pyloric gland regions of the hindstomach. The cardiac mucosa had smooth and folded areas; these were filled with mixed glands. In the fundic glands, the parietal cells outnumbered the chief cells. The pyloric glands were of serous-like in characteristics. In conclusion, gross and histological structures of the stomach of western grey kangaroo are adaptive with its food habitat, which allows thorough mixing of highly fibrous grasses.
Abd-Elmaksoud Ahmed, S. Ebada Mohamed & Mahmoud Badran Shoaib, 2012. Localisation of cytokeratin ... more Abd-Elmaksoud Ahmed, S. Ebada Mohamed & Mahmoud Badran Shoaib, 2012. Localisation of cytokeratin and smooth muscle actin in the accessory genital glands of camels (Camelus dromedarius) during rutting and non-rutting seasons. Bulg. J. Vet. Med., 15, No 4,2012 .The present study has disclosed for the first time the distribution of cytokeratin (CK) and α smooth muscle actin (α SMA) in the accessory genital glands of camel. In prostate, CK was localised in the cytoplasm of columnar cells of secretory acini and in the scanty cytoplasm of basal cells. In the ampulla of ductus deferens, the CK reaction was found in the pseudostratified columnar epithelium of mucosa and in the secretory columnar epithelium of submucosal glands. In the bulbourethral gland, CK reaction was exclusively observed in the pyramidal cells of type A and type C secretory units as well as in the lining epithelium of the duct system. α SMA was localised to the smooth muscle cells of the prostatic capsule, fibromuscular...
Journal of Veterinary Medical Researches (Print), Dec 12, 2017
Electron tomography is a new technique for obtaining detailed 3D structures of both biological an... more Electron tomography is a new technique for obtaining detailed 3D structures of both biological and non-biological samples relevant to the life and biomedical sciences. Electron tomography is an extension of the traditional transmission electron microscopy. Different protocols concerning the 3D electron tomography, Conventional TEM, Immuno-electron microscopy and cryoEM will be covered. With these methods, relatively large volumes of resinembedded biological structures can be analyzed at resolutions of a few nm within a reasonable expenditure of time. Therefore, this work could support the collaborative research activities of many investigators from various departments in the faculty of veterinary medicine whose work has already had a major impact in the areas of biological, life and physical sciences.
We describe the morphological, histological and histochemical characteristics of the uropygial gl... more We describe the morphological, histological and histochemical characteristics of the uropygial gland of the duck (Anas platyrhynchos) and pigeon (Columba livia). The weight and dimensions of the glands were recorded. The gland openings onto the papilla in ducks were surrounded by double tufts of downy feathers; the tufts were held together by an oily secretion. In the pigeon, the gland opening was onto naked skin. Samples for light and transmission electron microscopy were obtained from adult birds. Gland morphometry of the duck showed greater dimensions and relative gland weight than the pigeon. In both species the gland was lined by stratified epithelium, packed with secretary tubules and filled with oil droplets that were discharged into a central cavity. In the pigeon, the gland was branched and alveolar with a wide central lumen, whereas in the duck it was simple and branched tubular. Ultrastructural analysis of both species revealed that the fat globules tended to condense in the peripheral layers of the secretory and degenerative cells, and the nuclei exhibited pyknosis, which suggest the holocrine nature of the gland. Morphometric and histological analysis of the gland showed that the architecture is similar among birds; however, some species-specific differences suggest a functional correlation with the habitat.
Journal of Advanced Veterinary and Animal Research, 2015
The present study was aimed to investigate the morphology and histomorphometry of stomach and gas... more The present study was aimed to investigate the morphology and histomorphometry of stomach and gastric mucosa in western grey kangaroo (Macropus fuliginosus). The stomach was composed of three indistinctive separate parts namely sacciform forestomach, tubiform forestomach, and hindstomach. The tubiform forestomach was the main tubular section of the organ. The stomach had a compound lining. The non-glandular mucosa occupied the medial blind sac (MBS) of the sacciform forestomach; the layer covered about one-third of the tubiform forestomach (non-glandular region) and the entire length of the gastric sulcus. The glandular part lined the parietal blind sac (PBS) of sacciform forestomach and the cardiac gland region of tubiform forestomach as well as fundic and pyloric gland regions of the hindstomach. The cardiac mucosa had smooth and folded areas; these were filled with mixed glands. In the fundic glands, the parietal cells outnumbered the chief cells. The pyloric glands were of serous-like in characteristics. In conclusion, gross and histological structures of the stomach of western grey kangaroo are adaptive with its food habitat, which allows thorough mixing of highly fibrous grasses.
DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2014
The lyssa is a morphologically supporting structure situated along the median plan on the ventral... more The lyssa is a morphologically supporting structure situated along the median plan on the ventral surface of the apex of the tongue in some animals. The present study aimed to describe the morphological differences of the lyssa in carnivores (dog and cat) and camel using conformist macroscopic and microscopic methods as well as evaluation of its surgical resection in the dog and cat. The lyssa is a rod-shaped in dog, strip like structure in cat and ridge-like structure in camel. It showed straight course in dog and helical appearance in cat. In the studied species, the lyssa was formed from adipose tissue intermingled with irregular connective tissue. However, dense striated muscles fibers were seen in the caudal half of the lyssa in dog. Besides, few bundles of longitudinally running muscle fibers were observed in cat and camel. In dog, the lyssa was defined by a coarse sheath of connective tissue, this capsule was ill-defined in cat and absent in camel. In cat, a pyramidal extension of the adipose tissue of the lyssa was attached to intrinsic striated muscle of the tongue. Full function of the tongue could be achieved after surgical resection of lyssa in dogs and cats.
related peptide; Protein gene product 9.5. The fact that the splice variant form of choline acety... more related peptide; Protein gene product 9.5. The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in peripheral organs, including sensory ones, preferentially than the common type (cChAT) is well known. In the current study the possible functional significance of this variant in sensory neurons has been characterized immunohistochemically by investigating the pChAT-immunoreactivity (IR) in the trigeminal ganglia (TG) of the guinea pig. We documented an almost uniform distribution and a considerable number of pChAT-immunoreactivity of all trigeminal neurons. The size of pChAT-IR neurons varied from small to medium-size, although large-sized neurons also observed. Most pChAT reactivity was mainly in the cytoplasm with few number of pChAT-IR neurons had nuclear staining. Double immunofluorescent study showed that a great proportion of substance P (SP)- and calcitonin gene-related peptide (CGRP)-positive trigeminal cells showed pChAT-immunoreactivity, al...
We describe the morphological, histological and histochemical characteristics of the uropygial gl... more We describe the morphological, histological and histochemical characteristics of the uropygial gland of the duck (Anas platyrhynchos) and pigeon (Columba livia). The weight and dimensions of the glands were recorded. The gland openings onto the papilla in ducks were surrounded by double tufts of downy feathers; the tufts were held together by an oily secretion. In the pigeon, the gland opening was onto naked skin. Samples for light and transmission electron microscopy were obtained from adult birds. Gland morphometry of the duck showed greater dimensions and relative gland weight than the pigeon. In both species the gland was lined by stratified epithelium, packed with secretary tubules and filled with oil droplets that were discharged into a central cavity. In the pigeon, the gland was branched and alveolar with a wide central lumen, whereas in the duck it was simple and branched tubular. Ultrastructural analysis of both species revealed that the fat globules tended to condense in the peripheral layers of the secretory and degenerative cells, and the nuclei exhibited pyknosis, which suggest the holocrine nature of the gland. Morphometric and histological analysis of the gland showed that the architecture is similar among birds; however, some species-specific differences suggest a functional correlation with the habitat.
Journal of Animal and Veterinary Advances, Jun 23, 2014
The lyssa is a morphologically supporting structure situated along the median plan on the ventral... more The lyssa is a morphologically supporting structure situated along the median plan on the ventral surface of the apex of the tongue in some animals. The present study aimed to describe the morphological differences of the lyssa in carnivores (dog and cat) and camel using conformist macroscopic and microscopic methods as well as evaluation of its surgical resection in the dog and cat. The lyssa is a rod-shaped in dog, strip like structure in cat and ridge-like structure in camel. It showed straight course in dog and helical appearance in cat. In the studied species, the lyssa was formed from adipose tissue intermingled with irregular connective tissue. However, dense striated muscles fibers were seen in the caudal half of the lyssa in dog. Besides, few bundles of longitudinally running muscle fibers were observed in cat and camel. In dog, the lyssa was defined by a coarse sheath of connective tissue, this capsule was ill-defined in cat and absent in camel. In cat, a pyramidal extension of the adipose tissue of the lyssa was attached to intrinsic striated muscle of the tongue. Full function of the tongue could be achieved after surgical resection of lyssa in dogs and cats.
The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in periph... more The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in peripheral organs, including sensory ones, preferentially than the common type (cChAT) is well known. In the current study the possible functional significance of this variant in sensory neurons has been characterized immunohistochemically by investigating the pChAT-immunoreactivity (IR) in the trigeminal ganglia (TG) of the guinea pig. We documented an almost uniform distribution and a considerable number of pChATimmunoreactivity of all trigeminal neurons. The size of pChAT-IR neurons varied from small to medium-size, although large-sized neurons also observed. Most pChAT reactivity was mainly in the cytoplasm with few number of pChAT-IR neurons had nuclear staining. Double immunofluorescent study showed that a great proportion of substance P (SP)-and calcitonin gene-related peptide (CGRP)-positive trigeminal cells showed pChATimmunoreactivity, although those with SP outnumbered those with CGRP. The intracellular expression of pChAT (which differs from that of cChAT) probably reflecting a difference in the physiological roles between pChAT and cChAT in ACh production in distinct intracellular compartments. The present data suggest also that pChAT may play roles other than nociception and may be involved in the sensory functions of the TG neurons.
The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in periph... more The fact that the splice variant form of choline acetyltransferase (pChAT) is expressed in peripheral organs, including sensory ones, preferentially than the common type (cChAT) is well known. In the current study the possible functional significance of this variant in sensory neurons has been characterized immunohistochemically by investigating the pChAT-immunoreactivity (IR) in the trigeminal ganglia (TG) of the guinea pig. We documented an almost uniform distribution and a considerable number of pChATimmunoreactivity of all trigeminal neurons. The size of pChAT-IR neurons varied from small to medium-size, although large-sized neurons also observed. Most pChAT reactivity was mainly in the cytoplasm with few number of pChAT-IR neurons had nuclear staining. Double immunofluorescent study showed that a great proportion of substance P (SP)-and calcitonin gene-related peptide (CGRP)-positive trigeminal cells showed pChATimmunoreactivity, although those with SP outnumbered those with CGRP. The intracellular expression of pChAT (which differs from that of cChAT) probably reflecting a difference in the physiological roles between pChAT and cChAT in ACh production in distinct intracellular compartments. The present data suggest also that pChAT may play roles other than nociception and may be involved in the sensory functions of the TG neurons.
The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis... more The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal duct as well as the principal cells of the rabbit epididymis. In conclusion, the distribution of the S-100 proteins in the testis and epididymis might point out to its roles in the male reproduction.
Abstract: The present investigation was conducted to demonstrate S-100 protein in the testis and ... more Abstract: The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal...
The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis... more The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal duct as well as the principal cells of the rabbit epididymis. In conclusion, the distribution of the S-100 proteins in the testis and epididymis might point out to its roles in the male reproduction.
The present study was aimed to investigate the morphology and histomorphometry of stomach and gas... more The present study was aimed to investigate the morphology and histomorphometry of stomach and gastric mucosa in western grey kangaroo (Macropus fuliginosus). The stomach was composed of three indistinctive separate parts namely sacciform forestomach, tubiform forestomach, and hindstomach. The tubiform forestomach was the main tubular section of the organ. The stomach had a compound lining. The non-glandular mucosa occupied the medial blind sac (MBS) of the sacciform forestomach; the layer covered about one-third of the tubiform forestomach (non-glandular region) and the entire length of the gastric sulcus. The glandular part lined the parietal blind sac (PBS) of sacciform forestomach and the cardiac gland region of tubiform forestomach as well as fundic and pyloric gland regions of the hindstomach. The cardiac mucosa had smooth and folded areas; these were filled with mixed glands. In the fundic glands, the parietal cells outnumbered the chief cells. The pyloric glands were of serous-like in characteristics. In conclusion, gross and histological structures of the stomach of western grey kangaroo are adaptive with its food habitat, which allows thorough mixing of highly fibrous grasses.
Abd-Elmaksoud Ahmed, S. Ebada Mohamed & Mahmoud Badran Shoaib, 2012. Localisation of cytokeratin ... more Abd-Elmaksoud Ahmed, S. Ebada Mohamed & Mahmoud Badran Shoaib, 2012. Localisation of cytokeratin and smooth muscle actin in the accessory genital glands of camels (Camelus dromedarius) during rutting and non-rutting seasons. Bulg. J. Vet. Med., 15, No 4,2012 .The present study has disclosed for the first time the distribution of cytokeratin (CK) and α smooth muscle actin (α SMA) in the accessory genital glands of camel. In prostate, CK was localised in the cytoplasm of columnar cells of secretory acini and in the scanty cytoplasm of basal cells. In the ampulla of ductus deferens, the CK reaction was found in the pseudostratified columnar epithelium of mucosa and in the secretory columnar epithelium of submucosal glands. In the bulbourethral gland, CK reaction was exclusively observed in the pyramidal cells of type A and type C secretory units as well as in the lining epithelium of the duct system. α SMA was localised to the smooth muscle cells of the prostatic capsule, fibromuscular...
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