This experiment was carried out to compare heat tolerance of the local Sinai sheep and goats with... more This experiment was carried out to compare heat tolerance of the local Sinai sheep and goats with those of Nile delta breeds (Rahmani sheep and Baladi goats). Twenty animals (5 male of each group) were used in this study. The age of sheep and goats ranged between 12 -18 months. The coat of each animal (wool or hair) had been shorn to maintain its lengths constant at about 1.5 2 cm throughout the study period. All the animals were exposed daily to direct solar radiation (DSR) from 12:00 h to 15:00 h for 7 successive days in August. The averages ambient temperature and relative humidity were 31.75 o C and 46%, respectively during this study. Data on the following parameters were collected: rectal temperature (RT), respiration rate (RR), hemoglobin concentration (Hb), haematocrit value (Ht), thyroid hormones levels (T3 and T4) and acid-base balance (pH and HCO3) before and after exposure to DSR. Results indicated that, exposure of animals to DSR increased significantly (P<0.05) RT, ...
Postpartum resumption of ovarian activity under environmental stressors is highly important for d... more Postpartum resumption of ovarian activity under environmental stressors is highly important for determining subsequent fertility in camel. The current study aimed to investigate the relationship of relevant biochemical and hormonal parameters in the blood serum with regard to ovarian resumption of dromedary camel at early post-partum. Blood samples were collected from female dromedary camel weekly during the postpartum period for 9 successive weeks. Serum and plasma samples were assayed for the following biochemical and hormonal constituents, glucose, cholesterol (CH), triglycerides (TG), total protein (TP), urea, insulin-like growth factor-1 (IGF-1), non-esterified fatty acids (NEFA), lactate dehydrogenase (LDH), cortisol and triiodothyronine (T3). Glucose concentrations ranged between 92.6 and 96.2 mg/dL for the first 6 weeks postpartum, and increased to 106.8 mg/dL at week 7 postpartum (P < 0.01). The concentrations of CH followed almost the same manner during the first 7 weeks...
The influence of subcutaneous injections of silver nanoparticles (AgNPs) on rabbit performance, h... more The influence of subcutaneous injections of silver nanoparticles (AgNPs) on rabbit performance, hematological and biochemical parameters of blood, antioxidant status, and the residues of silver in meat and blood in two breeds (New Zealand White (NZW) and Jabali) of rabbits growing under high ambient temperature was evaluated. A total of 90 six-week-old rabbits (45 NZW and 45 Jabali) were randomly distributed into three equal treatment groups (control, 0.5 mg, and 1.0 mg AgNPs/kg body weight). The treated rabbits were injected twice a week for four consecutive weeks. The results revealed that AgNPs administration had no significant effect on average daily gain (ADG), feed intake, and feed conversion ratio (FCR). The NZW breed surpassed the Jabali breed in growth performance traits, carcass weight, dressing percentage, and cuts of mid parts and hind cuts. Administration of AgNPs had a significant effect on hematocrit (HCT) and platelet (PLT) values. Rabbits injected with AgNPs at a do...
Positive effect of Manganese (III) meso-tetrakis (4-benzoic acid) porphyrin on stallion spermatoz... more Positive effect of Manganese (III) meso-tetrakis (4-benzoic acid) porphyrin on stallion spermatozoa during storage in cool condition
High concentrations of non-esterified fatty acids (NEFA) during negative energy balance (NEB) in ... more High concentrations of non-esterified fatty acids (NEFA) during negative energy balance (NEB) in high yielding dairy cows have been proven to be partially responsible for reduced fertility. This hypothesis has been tested by the addition of NEFAs to in vitro maturation medium at concentrations present in follicular fluid during NEB. We aimed to evaluate whether high concentrations of palmitic acid (C16:0) (PA), stearic acid (C18:0) (SA), or oleic acid (C18:1) (OA) during oocyte maturation could have a carry-over effect on embryo quality and could subsequently affect embryo cryotolerance. Cumulus–oocyte complexes (n = 4600) were matured in serum-free TCM199 plus epidermal growth factor (EGF, 20 ng mL–1; negative control), supplemented with ethanol alone (positive control) or with 0.133 mmol L–1 PA, 0.067 mmol L–1 SA, or 0.200 mmol L–1 OA (NEFAs dissolved in ethanol). The three NEFAs were tested separately in 4 replicates for PA and 5 replicates for OA or SA. Each fatty acid tested pe...
Metabolic profile changes of the follicular fluid of the growing follicles can be used as an indi... more Metabolic profile changes of the follicular fluid of the growing follicles can be used as an indirect indicator of the oocyte and granulosa cells quality. The aim of this study was to investigate the biochemical compositions of follicular fluid collected from follicles at different stages of growth and their relationship with that of blood serum in Dromedary camel. Ovaries were colleceted from local slaughterhouse (Oniza, KSA). Soon after slaughtering, blood samples were collected from Dromedary camel (n = 20) and follicular fluid was aspirated from three different groups of non-atretic follicles (4-6)mm, 6-8mm and 10-20mm diameter). Follicular samples were pooled by maintaining the follicular sizes. Concentrations of glucose, cholesterol, triglycerides, urea, total protein, lactate dehydrosenase (LDH), cortisol, triiodothyronine (T3), insulin-like growth factor-1 (IGF-1) and non-esterified fatty acids (NEFA) were assayed in each serum and follicular fluid sample. The concentrations of glucose, cholesterol, triglycerides, total protein, LDH, T3, IGF-1 and NEFA decreased in the follicular fluid irrespective of follicular sizes and increasing in trend for urea in comparison with blood serum. There was a significant concentration gradient for IGF-1 in small follicular group compared to large or medium groups. Our data from the present study suggest that the oocyte and the granulosa cells of Dromedary she camel develop in a biochemical environment that does not have remarkable changes from small to large follicles except for IGF-1. In conclusion, the above-mentioned metabolic changes in the growing follicle is related to blood metabolic changes and, therefore, may be used in determining follicular dominance, oocyte and granulosa cells quality in Dromedary camel.
The current study aimed to investigate the circadian rhythm of blood metabolic parameters associa... more The current study aimed to investigate the circadian rhythm of blood metabolic parameters associated with summer heat stress (HS) in dairy cows. Ten healthy lactating Holstein Friesian cows were followed during HS for three successive days at six different time points. Blood was sampled from each cow starting from 07:00AM; at 4-h intervals. Ambient air temperature and relative humidity were recorded, and temperature-humidity index (THI) was calculated as well. Respiration rate (RR) and rectal temperature (RT) were recorded for each cow at the time of blood sampling. Concentrations of glucose, nonesterified fatty acids (NEFA), total cholesterol (TC) and urea were measured in each blood sample. The THI values
In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concen... more In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concentrations measured previously in follicular fluid (FF) of high-producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These in vitro results suggest that high NEFA concentrations in FF during a period of negative energy balance in high-yielding dairy cows can have carry-over effects on embryo quality.
This experiment was carried out to compare heat tolerance of the local Sinai sheep and goats with... more This experiment was carried out to compare heat tolerance of the local Sinai sheep and goats with those of Nile delta breeds (Rahmani sheep and Baladi goats). Twenty animals (5 male of each group) were used in this study. The age of sheep and goats ranged between 12 -18 months. The coat of each animal (wool or hair) had been shorn to maintain its lengths constant at about 1.5 2 cm throughout the study period. All the animals were exposed daily to direct solar radiation (DSR) from 12:00 h to 15:00 h for 7 successive days in August. The averages ambient temperature and relative humidity were 31.75 o C and 46%, respectively during this study. Data on the following parameters were collected: rectal temperature (RT), respiration rate (RR), hemoglobin concentration (Hb), haematocrit value (Ht), thyroid hormones levels (T3 and T4) and acid-base balance (pH and HCO3) before and after exposure to DSR. Results indicated that, exposure of animals to DSR increased significantly (P<0.05) RT, ...
Postpartum resumption of ovarian activity under environmental stressors is highly important for d... more Postpartum resumption of ovarian activity under environmental stressors is highly important for determining subsequent fertility in camel. The current study aimed to investigate the relationship of relevant biochemical and hormonal parameters in the blood serum with regard to ovarian resumption of dromedary camel at early post-partum. Blood samples were collected from female dromedary camel weekly during the postpartum period for 9 successive weeks. Serum and plasma samples were assayed for the following biochemical and hormonal constituents, glucose, cholesterol (CH), triglycerides (TG), total protein (TP), urea, insulin-like growth factor-1 (IGF-1), non-esterified fatty acids (NEFA), lactate dehydrogenase (LDH), cortisol and triiodothyronine (T3). Glucose concentrations ranged between 92.6 and 96.2 mg/dL for the first 6 weeks postpartum, and increased to 106.8 mg/dL at week 7 postpartum (P < 0.01). The concentrations of CH followed almost the same manner during the first 7 weeks...
The influence of subcutaneous injections of silver nanoparticles (AgNPs) on rabbit performance, h... more The influence of subcutaneous injections of silver nanoparticles (AgNPs) on rabbit performance, hematological and biochemical parameters of blood, antioxidant status, and the residues of silver in meat and blood in two breeds (New Zealand White (NZW) and Jabali) of rabbits growing under high ambient temperature was evaluated. A total of 90 six-week-old rabbits (45 NZW and 45 Jabali) were randomly distributed into three equal treatment groups (control, 0.5 mg, and 1.0 mg AgNPs/kg body weight). The treated rabbits were injected twice a week for four consecutive weeks. The results revealed that AgNPs administration had no significant effect on average daily gain (ADG), feed intake, and feed conversion ratio (FCR). The NZW breed surpassed the Jabali breed in growth performance traits, carcass weight, dressing percentage, and cuts of mid parts and hind cuts. Administration of AgNPs had a significant effect on hematocrit (HCT) and platelet (PLT) values. Rabbits injected with AgNPs at a do...
Positive effect of Manganese (III) meso-tetrakis (4-benzoic acid) porphyrin on stallion spermatoz... more Positive effect of Manganese (III) meso-tetrakis (4-benzoic acid) porphyrin on stallion spermatozoa during storage in cool condition
High concentrations of non-esterified fatty acids (NEFA) during negative energy balance (NEB) in ... more High concentrations of non-esterified fatty acids (NEFA) during negative energy balance (NEB) in high yielding dairy cows have been proven to be partially responsible for reduced fertility. This hypothesis has been tested by the addition of NEFAs to in vitro maturation medium at concentrations present in follicular fluid during NEB. We aimed to evaluate whether high concentrations of palmitic acid (C16:0) (PA), stearic acid (C18:0) (SA), or oleic acid (C18:1) (OA) during oocyte maturation could have a carry-over effect on embryo quality and could subsequently affect embryo cryotolerance. Cumulus–oocyte complexes (n = 4600) were matured in serum-free TCM199 plus epidermal growth factor (EGF, 20 ng mL–1; negative control), supplemented with ethanol alone (positive control) or with 0.133 mmol L–1 PA, 0.067 mmol L–1 SA, or 0.200 mmol L–1 OA (NEFAs dissolved in ethanol). The three NEFAs were tested separately in 4 replicates for PA and 5 replicates for OA or SA. Each fatty acid tested pe...
Metabolic profile changes of the follicular fluid of the growing follicles can be used as an indi... more Metabolic profile changes of the follicular fluid of the growing follicles can be used as an indirect indicator of the oocyte and granulosa cells quality. The aim of this study was to investigate the biochemical compositions of follicular fluid collected from follicles at different stages of growth and their relationship with that of blood serum in Dromedary camel. Ovaries were colleceted from local slaughterhouse (Oniza, KSA). Soon after slaughtering, blood samples were collected from Dromedary camel (n = 20) and follicular fluid was aspirated from three different groups of non-atretic follicles (4-6)mm, 6-8mm and 10-20mm diameter). Follicular samples were pooled by maintaining the follicular sizes. Concentrations of glucose, cholesterol, triglycerides, urea, total protein, lactate dehydrosenase (LDH), cortisol, triiodothyronine (T3), insulin-like growth factor-1 (IGF-1) and non-esterified fatty acids (NEFA) were assayed in each serum and follicular fluid sample. The concentrations of glucose, cholesterol, triglycerides, total protein, LDH, T3, IGF-1 and NEFA decreased in the follicular fluid irrespective of follicular sizes and increasing in trend for urea in comparison with blood serum. There was a significant concentration gradient for IGF-1 in small follicular group compared to large or medium groups. Our data from the present study suggest that the oocyte and the granulosa cells of Dromedary she camel develop in a biochemical environment that does not have remarkable changes from small to large follicles except for IGF-1. In conclusion, the above-mentioned metabolic changes in the growing follicle is related to blood metabolic changes and, therefore, may be used in determining follicular dominance, oocyte and granulosa cells quality in Dromedary camel.
The current study aimed to investigate the circadian rhythm of blood metabolic parameters associa... more The current study aimed to investigate the circadian rhythm of blood metabolic parameters associated with summer heat stress (HS) in dairy cows. Ten healthy lactating Holstein Friesian cows were followed during HS for three successive days at six different time points. Blood was sampled from each cow starting from 07:00AM; at 4-h intervals. Ambient air temperature and relative humidity were recorded, and temperature-humidity index (THI) was calculated as well. Respiration rate (RR) and rectal temperature (RT) were recorded for each cow at the time of blood sampling. Concentrations of glucose, nonesterified fatty acids (NEFA), total cholesterol (TC) and urea were measured in each blood sample. The THI values
In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concen... more In this study, non-esterified fatty acids (NEFAs) were added during in vitro maturation at concentrations measured previously in follicular fluid (FF) of high-producing dairy cows in a negative energy status to evaluate their subsequent effect on the embryos cryotolerance. Oocytes were matured for 24 h in serum-free media with or without (negative control) the addition of NEFAs dissolved in ethanol or ethanol alone (positive control). Matured oocytes were fertilized and cultured for 7 days in synthetic oviduct fluid medium supplemented with 5% FCS. Embryos that had at least reached the blastocyst stage were vitrified by open pulled straw (OPS) vitrification. Addition of palmitic (C16 : 0) or stearic acid (C18 : 0) during oocyte maturation had significant negative effects on embryo cryotolerance, whereas ethanol or oleic acid (C18 : 1) had no effect. These in vitro results suggest that high NEFA concentrations in FF during a period of negative energy balance in high-yielding dairy cows can have carry-over effects on embryo quality.
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