Papers by Manish Shandilya
Cancer Control, 2001
Background Pain affects more than 70% of cancer patients but is often undertreated. Methods The a... more Background Pain affects more than 70% of cancer patients but is often undertreated. Methods The authors review and present methodologies to maximize proper palliative approaches to this symptom for the majority of patients. Results The World Health Organization's stepwise guide to pain control serves as an excellent basis for management. Around-the-clock dosing, using adjuvant treatments, and using noninvasive routes of administration provide good pain control for 80% of patients. Conclusions Barriers to effective pain control will be reduced as new JCAHO standards regarding pain control are implemented.
<p>Schematic representation of possible unfolding pathways undertaken by α-amylase during t... more <p>Schematic representation of possible unfolding pathways undertaken by α-amylase during treatment with different denaturants.</p
<p>The enzymatic activity was estimated both at respective pH of incubation (red) and at pH... more <p>The enzymatic activity was estimated both at respective pH of incubation (red) and at pH 5.0 followed by incubation at various pH (black) (<b>B</b>) Changes in ellipticity at 222 nm with pH (<b>C</b>) pH dependent fluorescence spectra of α-amylase (<b>D</b>) Fluorescence intensity of ANS binding to α-amylase at different pH.</p
<p>(A) Native far-UV CD spectrum and (B) Fluorescence emission spectra at different excitat... more <p>(A) Native far-UV CD spectrum and (B) Fluorescence emission spectra at different excitation wavelengths of α-amylase reveal similar emission maximum (tryptophan, 340 nm) with higher energy emission at lower excitation wavelengths (as both tyrosine and tryptophan are excited) whereas lower emission at higher wavelength (only tryptophan being excited).</p
Advanced Spectroscopic Methods to Study Biomolecular Structure and Dynamics
Advances in Precision Medicine Oncology
Anthracyclines drugs are used as a treatment regime to combat cancer owing to their great chemoth... more Anthracyclines drugs are used as a treatment regime to combat cancer owing to their great chemotherapeutic potential. They are characterized by the presence of a wide range of derivatives, the most famous are doxorubicin and daunorubicin. The proposed action mechanism of anthracyclines and their derivatives to exert cytotoxic effect involves the intercalation of the drug molecule into nucleic acid and inhibition of the activity of topoisomerases. These events consequences in halting DNA replication and transcription mechanisms of the cell. Understanding of the structural and conformational changes associated with nucleic acid after binding with drugs provides significant knowledge for the development of more effective drugs. A comprehensive elucidation of the molecular mechanism(s) of action of anthracyclines drugs plays a significant role in the rational drug designing to obtain an effective, selective, and safe anti-cancer drugs.
<p>Thermal denaturation profiles of α-amylase (A) Residual enzymatic activity as a function... more <p>Thermal denaturation profiles of α-amylase (A) Residual enzymatic activity as a function of heat denaturation was estimated both at respective temperature of incubation (open circle) and at 68°C followed by incubation for 5 min at various temperatures (closed circle) (B) Far-UV CD spectra at various temperatures (C) Changes in ellipticity at 222 nm with temperature (D) Temperature-dependent fluorescence spectra of α-amylase.</p
<p>The enzyme displayed 13 tryptophan, 15 tyrosine and 16 phenylalanine side chains.</p
<p>Thermodynamic and kinetic parameters for unfolded α-amylase.</p
3 Biotech, 2022
Electrospinning is an electrostatic fiber fabrication technique that operates by the application ... more Electrospinning is an electrostatic fiber fabrication technique that operates by the application of a strong electric field on polymer solution or melts. It is used to fabricate fibers whose size lies in the range of few microns to the nanometer range. Historic development of electrospinning has evinced attention due to its outstanding attributes such as small diameter, excellent pore inter-connectivity, high porosity, and high surface-to-volume ratio. This review aims to highlight the theory behind electrospinning and the machine setup with a detailed discussion about the processing parameters. It discusses the latest innovations in natural protein-based electrospun nanofibers for health care applications. Various plant- and animal-based proteins have been discussed with detailed sample preparation and corresponding processing parameters. The usage of these electrospun nanofibers in regenerative medicine and drug delivery has also been discussed. Some technical innovations in electrospinning techniques such as emulsion electrospinning and coaxial electrospinning have been highlighted. Coaxial electrospun core–shell nanofibers have the potential to be utilized as an advanced nano-architecture for sustained release targeted delivery as well as for regenerative medicine. Healthcare applications of nanofibers formed via emulsion and coaxial electrospinning have been discussed briefly. Electrospun nanofibers have still much scope for commercialization on large scale. Some of the available wound-dressing materials have been discussed in brief.
Suggestions for Addressing Clinical and Non-Clinical Issues in Palliative Care, 2021
Depression is a serious concern among patients and their family members undergoing treatment for ... more Depression is a serious concern among patients and their family members undergoing treatment for acute and chronic illnesses. The quality of palliative care has great significance in improving the mental health of patients. The patient usually undergoes various stages of treatment before reaching the palliative care stage. Therefore, the tendency of denying treatment at later stages of illness increases. Depression could arise either early or at later stages of treatment. Most doctors feel restrained to prescribe antidepressants to the patient, as antidepressants could have a serious side effect on the physiology of the patient. Antidepressants only work for a smaller group of patients. A proper diagnostics and structured interview with extensive checks of reliability and viability could be used for assessment. Various factors like sociodemographic, gender, age, support health groups have a drastic effect on the mental health of the patient. Palliative care to the patients with soci...
International journal of biological macromolecules, 2021
The ubiquitous nature of hemoglobins, their presence in multiple forms and low cellular expressio... more The ubiquitous nature of hemoglobins, their presence in multiple forms and low cellular expression in organisms suggests alternative physiological functions of hemoglobins in addition to oxygen transport and storage. Previous research has proposed enzymatic function of hemoglobins such as nitric oxide dioxygenase, nitrite reductase and hydroxylamine reductase. In all these enzymatic functions, active ferrous form of hemoglobin is converted to ferric form and reconversion of ferric to ferrous through reduction partners is under active investigation. The model alga C. reinhardtii contains multiple globins and is thus expected to have multiple putative methemoglobin reductases to augment the physiological functions of the novel hemoglobins. In this regard, three putative methemoglobin reductases and three algal hemoglobins were characterized. Our results signify that the identified putative methemoglobin reductases can reduce algal methemoglobins in a nonspecific manner under in vitro ...
Assistive Technologies for Assessment and Recovery of Neurological Impairments, 2022
Assistive technology (AT) is defined as any device or technology ranging from low to high that he... more Assistive technology (AT) is defined as any device or technology ranging from low to high that helps to improve functional skills of individuals with cognitive, physical, or neurological disorders. These technologies are devised to improve the functional activities of persons. ATs focussing on cognitive disorders like dementia, autism spectrum disorders, etc. can be categorized as ATs for cognition (ATC) and augmentative and alternative communication (AAC). AAC interventions consist of tools that aid the challenges faced by individuals with speech impairment during communication. AACs used often are speech-generating devices (SGD), software programs, and communication apps for efficient production of speech. ATCs include social stories and video modeling strategies. The use of assistive technology in autism spectrum disorder (ASD) has great importance due to increased requirement for interventions in helping students. There are several lines of evidence showing the effectiveness of ...
International Journal of Biological Macromolecules, 2020
Understanding of interactions of nanomaterials with biomolecules (especially proteins) is of grea... more Understanding of interactions of nanomaterials with biomolecules (especially proteins) is of great importance to the area of nanobiotechnology. Graphene and its derivative such as graphene oxide (GO), are two-dimensional (2-D) nanomaterials with remarkable physical and chemical properties and have been broadly explored in biotechnology and biomedical application. Here, we have reported the nature of adsorption of trypsin on the GO surface, considering its biomedical implications. A simple incubation of trypsin on GO surface exhibits varying resistance to autolysis. The structural morphology of trypsin on the GO surface was studied by using atomic force microscopy (AFM), circular dichroism (CD), fluorescence, and total internal reflection fluorescence (TIRF) microscopies. Results suggest that the trypsin follows the Freundlich Isotherm. By the Langmuir model, the maximum adsorption capacity was found to be 100 mg/g. From protein assay results we have concluded that the native trypsin exhibits the highest catalytic efficiency (33.97*104 L mol-1 min-1) in comparison to other Trp-GO constructs. We have further visualized morphological change on GO-trypsin interface throughout the adsorption process by taking samples at definite time intervals, which suggests that the interaction of trypsin with GO is an example of the soft corona. Our findings may be implicated in enzyme engineering as well as enzyme-based bio-sensing applications.
Journal of Biomolecular Structure and Dynamics, 2019
Anthracyclines are putative anticancer agents used to treat a wide range of cancers. Among these ... more Anthracyclines are putative anticancer agents used to treat a wide range of cancers. Among these anthracyclines, epirubicin is derived from the doxorubicin by the subtle difference in the orientation of C4-hydroxyl group at sugar molecule. Epirubicin has great significance as it has propitious anticancer potential with lesser cardiotoxicity and faster elimination from the body.
PLOS ONE, 2015
Wheat α-amylase, a multi-domain protein with immense industrial applications, belongs to α +β cla... more Wheat α-amylase, a multi-domain protein with immense industrial applications, belongs to α +β class of proteins with native molecular mass of 32 kDa. In the present study, the pathways leading to denaturation and the relevant unfolded states of this multi-domain, robust enzyme from wheat were discerned under the influence of temperature, pH and chemical denaturants. The structural and functional aspects along with thermodynamic parameters for α-amylase unfolding were probed and analyzed using fluorescence, circular dichroism and enzyme assay methods. The enzyme exhibited remarkable stability up to 70°C with tendency to aggregate at higher temperature. Acid induced unfolding was also incomplete with respect to the structural content of the enzyme. Strong ANS binding at pH 2.0 suggested the existence of a partially unfolded intermediate state. The enzyme was structurally and functionally stable in the pH range 4.0-9.0 with 88% recovery of hydrolytic activity. Careful examination of biophysical properties of intermediate states populated in urea and GdHCl induced denaturation suggests that α-amylase unfolding undergoes irreversible and non-coincidental cooperative transitions, as opposed to previous reports of two-state unfolding. Our investigation highlights several structural features of the enzyme in relation to its catalytic activity. Since, α-amylase has been comprehensively exploited for use in a range of starch-based industries, in addition to its physiological significance in plants and animals, knowledge regarding its stability and folding aspects will promote its biotechnological applications.
Biophysical Journal, 2014
reporter. Using the second methods we found out that the UCS domain but not the central domain pr... more reporter. Using the second methods we found out that the UCS domain but not the central domain prevents the thermal aggregation of the myosin motor domain. We conclude that while both the UCS domain and the central domain bind the myosin head, only the UCS domain displays chaperone activity. Funded by a grant from the American Heart Association (AHA 13GRNT17290006).
PLoS ONE, 2011
Background: Human dopamine b-hydroxylase (DBH) is an important therapeutic target for complex tra... more Background: Human dopamine b-hydroxylase (DBH) is an important therapeutic target for complex traits. Several single nucleotide polymorphisms (SNPs) have also been identified in DBH with potential adverse physiological effect. However, difficulty in obtaining diffractable crystals and lack of a suitable template for modeling the protein has ensured that neither crystallographic three-dimensional structure nor computational model for the enzyme is available to aid rational drug design, prediction of functional significance of SNPs or analytical protein engineering.
International Journal of Biological Macromolecules, 2012
Chlorambucil (CMB) is an anticancer drug used for the treatment of variety of cancers. Structural... more Chlorambucil (CMB) is an anticancer drug used for the treatment of variety of cancers. Structural and conformational changes associated with DNA after binding with CMB were explored using spectroscopic techniques to get insight into the mechanism of action of CMB at molecular level. Different molar ratios of CMB-DNA complex were prepared with constant DNA concentration under physiological conditions. FTIR spectroscopy, UV-visible spectroscopy, CD spectroscopy and molecular docking studies were employed to determine the binding site and binding constant of CMB with DNA. The results show CMB binds DNA through nitrogenous bases (thymine, guanine and cytosine). The binding constant was calculated to be 1.3 × 10 3 M −1 , which suggests weak binding of CMB with DNA double helix. FTIR and CD results show that CMB do not disturb native B-conformation of DNA and it continues to remain in its B conformation even at higher concentrations of CMB. The molecular docking results are in corroboration with our experimental results and provides structural insight into the interaction site.
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Papers by Manish Shandilya