I LSEVIER Process Biochemistry Vol. 32, No. 7, pp. 573-578, 1997 1997 Elsevier Science Ltd All ri... more I LSEVIER Process Biochemistry Vol. 32, No. 7, pp. 573-578, 1997 1997 Elsevier Science Ltd All rights reserved. Printed in Great Britain 0032-9592/97 $17.00 + 0.00 Pit: S0032-9592(97)00018 -6 Bacterial attachment: its role in bioleaching processes S. Porro,a S. Ramirez,a C. ...
The serum response element is one of the major promoter elements of the immediate early response ... more The serum response element is one of the major promoter elements of the immediate early response to extracellular signals. The serum response element includes two main binding sites for proteins: the Ets box, which binds p62 TCF , and the CArG box, which binds p67 SRF . These two proteins are direct targets for signal transduction pathways; p62 TCF is a nuclear end point of the Ras/mitogen-activated protein kinase pathway, and p67 SRF is targeted by the Rho/Rac small G-proteins. The mechanism by which the signal is further transduced from the transcription factors to the basal transcriptional machinery is poorly understood. Recent data have suggested that the cAMP-responsive element-binding protein (CREB)-binding protein, a transcriptional adaptor involved in the transactivation through a wide variety of enhancer elements, participates in p62 TCF activity. We here show that the CREB-binding protein also cooperates in the process of transactivation by p67 SRF . Cotransfections of expression vectors for the CREBbinding protein increased the expression, in response to serum, of reporters under the control of the c-fos serum response element. Interestingly, the C-terminal moiety of the CREB-binding protein was not necessary to observe this effect. The cooperation did not require the Ets box in the serum response element, and the CArG box was sufficient, indicating that the CREB-binding protein is able to cooperate with p67 SRF in the absence of an Ets protein. Co-immunoprecipitation experiments using cell extracts showed that p67 SRF could be retained with antibodies directed against the CREB-binding protein, suggesting that the two proteins form a multimolecular complex in live cells. The physical interaction between p67 SRF and the CREB-binding protein was further confirmed by two-hybrid assays in mammalian cells. Our results indicate that the CREB-binding protein cooperates with p67 SRF and, thus, suggest that the serum response element is regulated by a multimolecular complex, which includes the CREB-binding protein, p67 SRF , and p62 TCF , with multiple interactions between the components of the complex.
Bioinformatics/computer Applications in The Biosciences, 2006
Motivation: In addition to existing bioinformatics software, a lot of new tools are being develop... more Motivation: In addition to existing bioinformatics software, a lot of new tools are being developed world wide to supply services for an ever growing, widely dispersed and heterogeneous collection of biological data. The integration of these resources under a common platform is a challenging task. To this end, several groups are developing integration technologies, in which services are usually registered in some sort of catalogue to allow novel discovering and accessing mechanisms to be implemented. However, each service demands specific interfaces to accommodate their parameters and it is a complicated task linking the different service inputs and outputs to solve a biological problem. Results: In this work we address the design and implementation of a versatile web client to access BioMOBY compatible services (a system by which a client can interact with multiple sources of biological data regardless of the underlying format or schema) using the service description stored in the BioMOBY catalogue. The automatic interface generator significantly reduces developing time and produces uniform service access mechanisms. The design and proof of concept (for such a Client) including the generic interface generator have been developed and implemented in the National Institute for Bioinformatics in Spain. by guest on January 21, 2015 http://bioinformatics.oxfordjournals.org/ Downloaded from I. Navas et al.
Conjunto de elementos humanos y materiales con sus atributos, ordenados para alcanzar un objetivo... more Conjunto de elementos humanos y materiales con sus atributos, ordenados para alcanzar un objetivo (de la empresa) de acuerdo con un esquema preciso de dependencias o relaciones internas (entre ellos) y externas (con el entorno).
I LSEVIER Process Biochemistry Vol. 32, No. 7, pp. 573-578, 1997 1997 Elsevier Science Ltd All ri... more I LSEVIER Process Biochemistry Vol. 32, No. 7, pp. 573-578, 1997 1997 Elsevier Science Ltd All rights reserved. Printed in Great Britain 0032-9592/97 $17.00 + 0.00 Pit: S0032-9592(97)00018 -6 Bacterial attachment: its role in bioleaching processes S. Porro,a S. Ramirez,a C. ...
The serum response element is one of the major promoter elements of the immediate early response ... more The serum response element is one of the major promoter elements of the immediate early response to extracellular signals. The serum response element includes two main binding sites for proteins: the Ets box, which binds p62 TCF , and the CArG box, which binds p67 SRF . These two proteins are direct targets for signal transduction pathways; p62 TCF is a nuclear end point of the Ras/mitogen-activated protein kinase pathway, and p67 SRF is targeted by the Rho/Rac small G-proteins. The mechanism by which the signal is further transduced from the transcription factors to the basal transcriptional machinery is poorly understood. Recent data have suggested that the cAMP-responsive element-binding protein (CREB)-binding protein, a transcriptional adaptor involved in the transactivation through a wide variety of enhancer elements, participates in p62 TCF activity. We here show that the CREB-binding protein also cooperates in the process of transactivation by p67 SRF . Cotransfections of expression vectors for the CREBbinding protein increased the expression, in response to serum, of reporters under the control of the c-fos serum response element. Interestingly, the C-terminal moiety of the CREB-binding protein was not necessary to observe this effect. The cooperation did not require the Ets box in the serum response element, and the CArG box was sufficient, indicating that the CREB-binding protein is able to cooperate with p67 SRF in the absence of an Ets protein. Co-immunoprecipitation experiments using cell extracts showed that p67 SRF could be retained with antibodies directed against the CREB-binding protein, suggesting that the two proteins form a multimolecular complex in live cells. The physical interaction between p67 SRF and the CREB-binding protein was further confirmed by two-hybrid assays in mammalian cells. Our results indicate that the CREB-binding protein cooperates with p67 SRF and, thus, suggest that the serum response element is regulated by a multimolecular complex, which includes the CREB-binding protein, p67 SRF , and p62 TCF , with multiple interactions between the components of the complex.
Bioinformatics/computer Applications in The Biosciences, 2006
Motivation: In addition to existing bioinformatics software, a lot of new tools are being develop... more Motivation: In addition to existing bioinformatics software, a lot of new tools are being developed world wide to supply services for an ever growing, widely dispersed and heterogeneous collection of biological data. The integration of these resources under a common platform is a challenging task. To this end, several groups are developing integration technologies, in which services are usually registered in some sort of catalogue to allow novel discovering and accessing mechanisms to be implemented. However, each service demands specific interfaces to accommodate their parameters and it is a complicated task linking the different service inputs and outputs to solve a biological problem. Results: In this work we address the design and implementation of a versatile web client to access BioMOBY compatible services (a system by which a client can interact with multiple sources of biological data regardless of the underlying format or schema) using the service description stored in the BioMOBY catalogue. The automatic interface generator significantly reduces developing time and produces uniform service access mechanisms. The design and proof of concept (for such a Client) including the generic interface generator have been developed and implemented in the National Institute for Bioinformatics in Spain. by guest on January 21, 2015 http://bioinformatics.oxfordjournals.org/ Downloaded from I. Navas et al.
Conjunto de elementos humanos y materiales con sus atributos, ordenados para alcanzar un objetivo... more Conjunto de elementos humanos y materiales con sus atributos, ordenados para alcanzar un objetivo (de la empresa) de acuerdo con un esquema preciso de dependencias o relaciones internas (entre ellos) y externas (con el entorno).
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