Papers by Selvaraju Sellappan
The Indian journal of animal sciences
The Indian journal of animal sciences
Proteins present in the seminal plasma and sperm influence sperm function and fertilization. The ... more Proteins present in the seminal plasma and sperm influence sperm function and fertilization. The present study was carried out to characterize the electrophoretic profile of bull sperm membrane proteins and to correlate with bull fertility. Semen samples were collected from 20 breeding bulls (10 Jersey and 10 Jersey crossbred) and the sperm membrane proteins were extracted by Triton X. Heparin binding proteins were eluted with heparin column, and the molecular weight of the proteins was assessed by discontinuous sodium dodecyl sulphate polyacrylamide gel elecrophoresis (SDS-PAGE). To assess the field fertility of the bulls, 50 frozen semen straws/bull were used for insemination. Results indicated that only 50 % of the bulls screened had 28-30 kDa heparin binding proteins in their sperm and these bulls had 12 % higher conception than the bulls lack the protein. So, it can be concluded that the bulls positive for of 28-30 kDa heparin binding proteins in sperm membrane protein had higher chance of fertility and screening for its presence can be used as a tool in addition to routine criteria for selecting bulls.
The Indian Journal of …, 2011
The role of reduced glutathione in improving the post thaw seminal characters in good and poor fr... more The role of reduced glutathione in improving the post thaw seminal characters in good and poor freezable Jersey crossbred bull semen was studied and compared with the control group. Two test groups, the test group each contains 18 ejaculates in both good ...
Karanja (Pongamia sp.) is a forest tree grown in parts of Andhra Pradesh, Karnataka and Tamil Nad... more Karanja (Pongamia sp.) is a forest tree grown in parts of Andhra Pradesh, Karnataka and Tamil Nadu. The seed kernels after extraction of oil contain good amount protein (around 30 % CP). However, raw karanj cake (rKC) is not commonly used as a feed for livestock and poultry due to its poor intake and toxic factors, i.e.furanoflavones like karanjin, pongamol and certain other polyphenolic compounds. After defatting and detoxification, the karanja cake can be a good source of protein and can replace costly conventional soybean meal (SBM). The objective of the present study was to assess the different levels of detoxified karanja cake (dKC) replacing SBM on sperm velocity parameters. The rams were randomly divided into four groups (n=6) and were fed different levels (%) of karanja cake (0% replacement, control; 25% replacement, dKC-25; 50% replacement, dKC-50 and 75% replacement, dKC-75) and fed for minimum of 60 days. After 50 days of feeding, the semen from the animals was collected ...
Human Reproduction Update, 2013
† Introduction † Methods † Spermatozoal RNAs-Characterization and potential roles Considerations ... more † Introduction † Methods † Spermatozoal RNAs-Characterization and potential roles Considerations Coding RNAs Small noncoding RNAs 'Other' sperm RNAs † Spermatozoal RNA as epigenetic modifiers † Models in Agriculture † Biomarkers of human fertility † Conclusion background: Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different processes including genome recognition, consolidation-confrontation, early embryonic development and epigenetic transgenerational inherence. Spermatozoal RNAs also provide a window into the developmental history of each sperm thereby providing biomarkers of fertility and pregnancy outcome which are being intensely studied. methods: Literature searches were performed to review the majority of spermatozoal RNA studies that described potential functions and clinical applications with emphasis on Next-Generation Sequencing. Human, mouse, bovine and stallion were compared as their distribution and composition of spermatozoal RNAs, using these techniques, have been described. results: Comparisons highlighted the complexity of the population of spermatozoal RNAs that comprises rRNA, mRNA and both large and small non-coding RNAs. RNA-seq analysis has revealed that only a fraction of the larger RNAs retain their structure. While rRNAs are the most abundant and are highly fragmented, ensuring a translationally quiescent state, other RNAs including some mRNAs retain their functional potential, thereby increasing the opportunity for regulatory interactions. Abundant small non-coding RNAs retained in spermatozoa include miRNAs and piRNAs. Some, like miR-34c are essential to the early embryo development required for the first cellular division. Others like the piRNAs are likely part of the genomic dance of confrontation and consolidation. Other non-coding spermatozoal RNAs include transposable elements, annotated lnc-RNAs, intronic retained elements, exonic elements, chromatin-associated RNAs, small-nuclear ILF3/NF30 associated RNAs, quiescent RNAs, mse-tRNAs and YRNAs. Some non-coding RNAs are known to act as epigenetic modifiers, inducing histone modifications † Reproductive Medicine Network.
Reproductive Biology, 2020
This experiment was conducted to study the effect of mineral supplementation on seminal plasma mi... more This experiment was conducted to study the effect of mineral supplementation on seminal plasma minerals level, biochemical constituents and total antioxidant capacity of Osmanabadi bucks. The study comprised of forty healthy bucks, aged five months were randomly assigned to ten groups (n = 4 per group). The control group was fed with a basal diet without any additional mineral supplementation. In addition to basal diet, treatment bucks were supplemented with three graded doses of organic Zinc (Zn) as 20, 40 and 60 mg/kg dry matter (DM); organic Copper (Cu) as 12.5, 25, 37.5 mg/ kg DM and combination of Zn + Cu as Zn20+Cu12.5, Zn40+Cu25, Zn60+Cu37.5 mg /kg DM basis respectively. Minerals were supplemented for 8 months and the separated seminal plasma used for analysis of minerals, biochemical profile, total antioxidant capacity (TAC), lipid peroxidation (LPO), and protein carbonylation (PC). In treatment groups, significantly lower LPO and PC were observed, except Zn60 and Zn60+Cu37.5, where higher malondialdehyde (MDA) (P < 0.05) formed. The TAC was relatively higher (P < 0.05) in Zn20, Zn40, Cu12.5 and Zn60+Cu37.5 than control. The minerals and biochemical parameters were significantly altered and positive relationship was observed among them. From this study, it was concluded that supplemented minerals changed the seminal plasma minerals profile (Zn-7-13; Cu-0.5-1.9 mg/L), reduced the stress (LPO and PC of control Vs treatment as 0.3 Vs 0.1 nmol/ml and 25.7 Vs 4.3 nmol protein carbonyl/mg protein), which improved the sperm quality in Zn40, all Cu treatments and Zn60+Cu37.5 groups respectively.
Reproduction in Domestic Animals, 2018
Onset of puberty, semen quality, fertility and overall livestock production are highly influenced... more Onset of puberty, semen quality, fertility and overall livestock production are highly influenced by mineral supplementation with high impacts on production efficiency (Rahman, Qureshi, & Khan, 2014). Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. More influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn)
Biology of Reproduction, 2011
Environmental Science and Pollution Research, 2016
The arsenic (As) is a multi system effector including reproduction. The present study examined th... more The arsenic (As) is a multi system effector including reproduction. The present study examined the association of graded doses of As(V) on testicular microenvironment and sperm function in mice. Thirty-six adult male mice were randomly assigned to six groups (n = 6). Group A served as control without test chemical. The groups B, C, D, E, and F were administered graded doses of 10, 25, 50, 100, and 200 ppm As(V), respectively, through drinking water for 40 days. A dose-dependant significant (P < 0.05) decrements were observed in epididymal sperm kinematic attributes (progressive motility, rapid, fast progressive, VCL, VSL, VAP, LIN, STR, WOB and TYPE A (STR >80 %, ALH 2.5 μm) by CASA), viability, plasma membrane functional integrity, and mitochondrial membrane potential which were associated with insignificant decrease in serum testosterone levels. The histoarchitectural studies of testes showed progressive loss of spermatozoa concentration in the seminiferous tubules as the As(V) dose increased. The mice exposed to As(V) had an increase in the As accumulation, protein carbonylation, and lipid peroxidation levels associated with alterations in SOD, CAT, and GST activities in the testes. In conclusion, higher doses of As(V) (more than 50 ppm) were found to be testicular toxicants which impaired semen quality by inducing oxidative stress in the testicular microenvironment.
The Indian journal of animal sciences
ABSTRACT
The Indian veterinary journal
ABSTRACT
The Indian journal of animal sciences
ABSTRACT
The Indian veterinary journal
ABSTRACT
The Indian journal of animal sciences
In the present experiment, 21 rams of uniform body weight and age (~ 1 year) were equally divided... more In the present experiment, 21 rams of uniform body weight and age (~ 1 year) were equally divided into 3 groups to find out the effect of 3 levels of energy, viz. control, low and high energy on growth, nutrient utilization, rumen biochemical profiles and semen quality. Body weight gain increased significantly compared to control and low energy. FCR improved significantly in high energy supplemented groups. Intake of DDM, DOM and TDN were significantly higher in high energy and control groups as compared to low energy group, with similar intake of CP and DCP in all groups. N-balance (g/d) was significantly higher in high energy group as compared to low energy group. Fractionation of VFA revealed a shift in propionate with narrower acetate to propionate ratio in high energy group favoring more body weight gain. Significantly higher semen volume (ml), mass activity (0–5 score) and progressive forward motility (%) were observed in optimum energy group compared to low energy group with ...
Reproduction, Fertility and Development, 2016
The buffalo seminal plasma protein profile and its relationship with sperm quality have not been ... more The buffalo seminal plasma protein profile and its relationship with sperm quality have not been studied in detail. Thus, the aim of the present study was to profile buffalo seminal plasma proteins and to assess the relationship between differentially expressed proteins and sperm characteristics. Semen samples (n = 44) were collected from 11 Murrah buffalo bulls (four ejaculates from each animal) and seminal plasma protein profiling was performed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Matrix-assisted laser desorption ionisation time-of-flight analysis of one of the differentially expressed proteins, namely the 11–12 kDa protein, identified it as tuberoinfundibular peptide of 39 residues (TIP39). Western blot analysis confirmed the presence of TIP39, with TIP39 expression in seminal plasma varying among bulls. Based on TIP39 levels, bulls were classified into two groups, those with high and low protein. The percentages of spermatozoa positive for mitochondrial ...
Analytical biochemistry, Jan 27, 2015
Sperm RNA can be used to understand the past spermatogenic process, future successful fertilizati... more Sperm RNA can be used to understand the past spermatogenic process, future successful fertilization and embryo development. In order to study the sperm RNA composition and function, isolation of good quality RNA with sufficient quantity is essential. The objective of this study is to assess the influence of sperm input concentrations and RNA isolation methods on RNA yield and quality in bull sperm. The fresh semen samples from bulls (n=6) were snap frozen in liquid nitrogen and stored at -80(0)C. The sperm RNA were isolated using membrane based methods combined with TRIzol (RNeasy+ TRIzol and PureLink + TRIzol) and conventional methods (TRIzol, Double TRIzol and RNAzol RT). Based on fluorometric quantification, combined methods resulted in significantly (p<0.05) higher total RNA yield (800-900 ng/30-40 x10(6)) as compared to other methods and yielded 20-30 fg of RNA/spermatozoon. The quality of RNA isolated by membrane based methods was superior to conventional methods. The sperm...
Uploads
Papers by Selvaraju Sellappan