(-)-Fisetinidol-(4 alpha,8)-[(-)-fisetinidol-(4 alpha,6)]-(+)-catechin (1), a proanthocyanidin, w... more (-)-Fisetinidol-(4 alpha,8)-[(-)-fisetinidol-(4 alpha,6)]-(+)-catechin (1), a proanthocyanidin, was isolated from the bark of Acacia leucophloea. Its structure including absolute configuration was elucidated on the basis of spectroscopic analysis and chemical correlation. The H-1 NMR spectrum of this compound, exhibiting exceptional complex signals attributable to rotational isomerism, and the reported data were obtained at elevated temperature in methyl ether acetate form. This work provided the H-1 and C-13 NMR assignments for 1 and its rotational isomer as the free phenolic form at ambient temperature for the first time. Compound 1 showed inhibitory activity against alpha-glucosidase type IV from Bacillus stearothermophilus with the IC50 value of 102.3 mu M
Teucrium yemense, a medicinal plant commonly grown in Saudi Arabia and Yemen, is traditionally us... more Teucrium yemense, a medicinal plant commonly grown in Saudi Arabia and Yemen, is traditionally used to treat infections, kidney diseases, rheumatism, and diabetes. Extraction of the dried aerial parts of the plant with methanol, followed by further extraction with butanol and chromatography, gave twenty novel neoclerodanes. Their structures, relative configurations and some conformations were determined by MS and 1-D and 2-D NMR techniques. Most were fairly conventional but one contained an unusual stable orthoester, one had its (C-16)-(C-13)-(C-14)-(C-15) (tetrahydro)furan unit present as a succinic anhydride and one had a rearranged carbon skeleton resulting from ring-contraction to give a central octahydroindene bicyclic core, rather than the usual decalin. Mechanisms are proposed for the biosynthetic formation of the orthoester and for the ring-contraction. Four novel neoclerodanes increased the glucose-triggered release of insulin from isolated murine pancreatic islets by more ...
Introduction: Multidrug-resistant bacteria are rapidly increasing worldwide, increasing antibioti... more Introduction: Multidrug-resistant bacteria are rapidly increasing worldwide, increasing antibiotic resistance. The exploitation, misuse, overuse, and decrease of therapeutic potential of currently available antibiotics have resulted in the development of resistance against bacteria. As the most common bacterial pathogen in humans, Staphylococcus aureus can cause many adverse health effects. In fighting multidrug resistant Staphylococcus aureus, scientists have identified an extremely relevant target - SaTMPK. SaTMPK is essential for DNA synthesis, which in turn is necessary for replication, cell division, of bacteria. Objective: To perform multistage screening using the ZINC database, followed by molecular docking, ADMET profiling, molecular dynamics simulations, and energy calculations. Methods: Based on the similar pharmacophoric characteristics of existing SaTMPK crystal structures, a model of interaction-based pharmacophores was developed. We then performed molecular docking stu...
The quality of groundwater has been degrading due to municipal sewage, industrial pollutants, fer... more The quality of groundwater has been degrading due to municipal sewage, industrial pollutants, fertilizers, herbicides, and pesticides. These dangerous pollutants enter into the deeper soil layers, infiltrate some aquifers, and decrease the gradation of groundwater. Other problems are associated with the leakage of sewer, faulty septic tank cleaning, landfill leachates, throwing of garbage into the river, pond and soil pollution. In coastal areas, salt water intrudes into freshwater aquifers due to intensive pumping of fresh groundwater. In the present study, the city Kashipur in the state of Uttarakhand has been chosen due to big industrial settlements. The industrial wastes contain many highly harmful elements which destroy the quality of groundwater in the adjacent areas. Therefore, it is our target to test the groundwater quality of Kashipur industrial areas. To analyze the groundwater and to assess the impact of groundwater pollution of Kashipur area, an attempt has been made in the present study to test the physicochemical parameters including pH, total hardness, alkalinity, chloride, fluoride, sulphate, iron, zinc, copper and heavy metal atoms such as lead, arsenic etc. Imbalance of these parameters may degrade the quality of groundwater and may be deleterious to the health of individual and society in context of drinking, agriculture and industrial purposes. Physiochemical treatment of groundwater samples collected in summer, rainy season and post-monsoon were compared and analyzed by regression modeling making a quantitative correlation between pH and other parameters including total hardness, alkalinity, chloride, sulfate, fluoride and copper using multiple linear regression methods.
The Reliability Prediction is an important tool for designing, decision making and estimating fut... more The Reliability Prediction is an important tool for designing, decision making and estimating future system success. Design engineers are often required to develop and estimate Reliability before the product is produced. Inaccurate predictions can lead to over design and/or excessive spare parts procurement. This work is based on the study of Reliability Analysis carried out on Electronic Communication Systems used in the aircraft avionics. This system was applied in the beginning for the Secure Speech Equipment designed specifically to encrypt voices as well as for fax and computer data. The Part Stress Analysis modeling is used in this study which is a worldwide standard for performing reliability predictions. The Reliability Block diagram is also developed as a tool for reliability prediction.
Supplemental material, Supplementary_Information_of_scoparia_revised_updated for Isolation and ch... more Supplemental material, Supplementary_Information_of_scoparia_revised_updated for Isolation and characterization of cytotoxic and anti-inflammatory constituents from Scoparia dulcis L by Mohammad Nur-e-Alam, Sarfaraz Ahmed, Muhammad Yousaf, Shabana I Khan, Ramzi A Mothana and Adnan J Al-Rehaily in Journal of Chemical Research
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Journal of Evolution of Medical and Dental Sciences, Mar 25, 2013
Extended spectrum beta lactamase (ESBL) producing Klebsiella spp have emerged as an important pat... more Extended spectrum beta lactamase (ESBL) producing Klebsiella spp have emerged as an important pathogen due to their high resistance against most of the antibiotics. Their detection and antibiotic resistance pattern is required for proper management of cases. In this study we report the prevalence and antibiotic resistance of such Klebsiella isolates. METHODS: A total of 100 clinical isolates of Klebsiella from different clinical samples were tested for ESBL production by double disc approximation test and CLSI phenotypic method. Antibiotic susceptibility of all the Klebsiella isolates were performed by Kirby-Bauer disc diffusion test. RESULTS: of 100 Klebsiella isolates 53 showed ESBL production. All ESBL producers were resistant to beta lactam antibiotics. Antibiotic resistance among ESBL producing strains was high as compared to non ESBL producing strains. INTERPRETATION & CONCLUSION: In the present study a large number of Klebsiella spp. isolated were found to be ESBL producers. Continuing monitoring of ESBL production and antimicrobial susceptibility testing is essential to avoid treatment failure.
2018 IEEE International Workshop on Signal Processing Systems (SiPS), 2018
Here, we propose a low cost, energy efficient embedded solution for the management of solid wasta... more Here, we propose a low cost, energy efficient embedded solution for the management of solid wastage. The core of this solution is a portable device consisting of an ultrasonic sensor, a processor (NodeMCU) with integrated transmitter for communicating to cloud server (firebase) and a power source (battery). The device comes with most of the information (e.g. unique identity) preprogrammed into its processor meaning the end user will need little to no manual interventions. To increase battery life, intelligent wake-up system has been implemented into the device that significantly reduces the power consumption of the system. The proposed solution will be beneficial to authority responsible for cleaning as they can now track the place where they should go and clean first. Municipalities can also get the statistical information about the amount of garbage produced in the city.
Increasing prevalence of methicillin resistant S. aureus (MRSA) and resistanceto multiple antibio... more Increasing prevalence of methicillin resistant S. aureus (MRSA) and resistanceto multiple antibiotic classes is a global issue. Regional surveillance of antibiotic susceptibility of the organism is a necessary step to overcome the issues of antimicrobial resistance and treatment failure in MRSA infections. The study was conducted to find the pattern of antibiotic susceptibility in MRSA isolated from the pus samples of patients attending a tertiary care hospital in Bastar tribal region in Central India. The study was performed on 215 MRSA isolates cultured from pus samples of patients over a period of two years and five months. In the methicillin resistant organisms selected by oxacillin screen agar test and cefoxitin disk diffusion test, antibiotic susceptibility was determined by Kirby Baur disk diffusion test with CLSI guide lines. Of the total S. aureus isolates, the incidence of MRSA was 34.1% of which 82.8% were resistant to coâ€trimoxazole, 77.2% to tetracycline, 68.8% to gen...
Abstract An orthopedic implant is a medical device used for treating the disability caused by var... more Abstract An orthopedic implant is a medical device used for treating the disability caused by various bone trauma such as fracture and also used to replace a missing joint or to provide support to the affected bone. The orthopedic implants have evolved through various generations. The recent developments in implants are focused on tissue-implant responses and the ways to answer them. The present chapter gives a comprehensive overview of the implants with respect to their history, materials used in the manufacture of implants, and associated properties of the materials. An emphasis is also laid on the complications associated with the implantation and methods to get past them both by tweaking the properties (increasing the biocompatibility) and manufacturing method. The required properties of the materials are also discussed in detail along with their evolution from metals to ceramics. Fabrication techniques including electrostatic spray deposition (ESD) and fiber laser surface engineering are also discussed with a proper focus on the entire design process involving verification, validation, and transfer. Currently, for the manufacture of orthopedic implants, directives of different countries, for example, European Union, United States, and Japan, are being followed. This chapter provides an overview of various implant materials used in orthopedics, various generations, and the vital properties critical for the selection of implant materials. This chapter also gives an idea about the design and development and manufacturing of orthopedic implants. The complications associated with the performance of the implant materials are also addressed to a sufficient extent in this chapter along with regulatory approval requirements, clinical trials, and current market status.
In the present study the dried material of Vernonia schimperi (Syn. Vernonia abyssinica Sch. Bip.... more In the present study the dried material of Vernonia schimperi (Syn. Vernonia abyssinica Sch. Bip. ex Walp.) was successively extracted with acetone and 96% ethanol. These extracts were further fractionated with different solvents and were analyzed for their phytoconstituents using standard protocols. From the study it was found that alkaloids and terpenoids are totally absent while the test for phenols found positive in all the fractions. Hexane fractions as well as acetonitrile fraction of one minute acetone extract showed the presence of glycosides. The sterols are only present in hexane fraction of one minute acetone extract. Most of the constituents are present in polar solvent fractions. Over all plant is rich in pharmaceutically important phytochemicals.
2018 IEEE/ACIS 17th International Conference on Computer and Information Science (ICIS), 2018
Bangladesh suffers more for being susceptible to flood driven loses than relishing the natural ad... more Bangladesh suffers more for being susceptible to flood driven loses than relishing the natural advantages of being a riverine country. It is crucial for flood management department to identify the severity of flood and the factors behind it. Though three main contributors to flood occurrences are the Brahmaputra, Ganges and Meghna river basins, there are several factors such as rainfall, location, elevation and many more that also has prominent influences in causing flood in different regions of Bangladesh. This paper intends to identify the flood condition of different regions of Bangladesh by implementing different clustering techniques such as K-means, Expectation Maximization and Agglomerative Hierarchical clustering algorithm. This paper aims not only to analyze the factors that lead to flood condition but also observe the effectiveness of applying the chosen clustering methods for further analysis.
(-)-Fisetinidol-(4 alpha,8)-[(-)-fisetinidol-(4 alpha,6)]-(+)-catechin (1), a proanthocyanidin, w... more (-)-Fisetinidol-(4 alpha,8)-[(-)-fisetinidol-(4 alpha,6)]-(+)-catechin (1), a proanthocyanidin, was isolated from the bark of Acacia leucophloea. Its structure including absolute configuration was elucidated on the basis of spectroscopic analysis and chemical correlation. The H-1 NMR spectrum of this compound, exhibiting exceptional complex signals attributable to rotational isomerism, and the reported data were obtained at elevated temperature in methyl ether acetate form. This work provided the H-1 and C-13 NMR assignments for 1 and its rotational isomer as the free phenolic form at ambient temperature for the first time. Compound 1 showed inhibitory activity against alpha-glucosidase type IV from Bacillus stearothermophilus with the IC50 value of 102.3 mu M
Teucrium yemense, a medicinal plant commonly grown in Saudi Arabia and Yemen, is traditionally us... more Teucrium yemense, a medicinal plant commonly grown in Saudi Arabia and Yemen, is traditionally used to treat infections, kidney diseases, rheumatism, and diabetes. Extraction of the dried aerial parts of the plant with methanol, followed by further extraction with butanol and chromatography, gave twenty novel neoclerodanes. Their structures, relative configurations and some conformations were determined by MS and 1-D and 2-D NMR techniques. Most were fairly conventional but one contained an unusual stable orthoester, one had its (C-16)-(C-13)-(C-14)-(C-15) (tetrahydro)furan unit present as a succinic anhydride and one had a rearranged carbon skeleton resulting from ring-contraction to give a central octahydroindene bicyclic core, rather than the usual decalin. Mechanisms are proposed for the biosynthetic formation of the orthoester and for the ring-contraction. Four novel neoclerodanes increased the glucose-triggered release of insulin from isolated murine pancreatic islets by more ...
Introduction: Multidrug-resistant bacteria are rapidly increasing worldwide, increasing antibioti... more Introduction: Multidrug-resistant bacteria are rapidly increasing worldwide, increasing antibiotic resistance. The exploitation, misuse, overuse, and decrease of therapeutic potential of currently available antibiotics have resulted in the development of resistance against bacteria. As the most common bacterial pathogen in humans, Staphylococcus aureus can cause many adverse health effects. In fighting multidrug resistant Staphylococcus aureus, scientists have identified an extremely relevant target - SaTMPK. SaTMPK is essential for DNA synthesis, which in turn is necessary for replication, cell division, of bacteria. Objective: To perform multistage screening using the ZINC database, followed by molecular docking, ADMET profiling, molecular dynamics simulations, and energy calculations. Methods: Based on the similar pharmacophoric characteristics of existing SaTMPK crystal structures, a model of interaction-based pharmacophores was developed. We then performed molecular docking stu...
The quality of groundwater has been degrading due to municipal sewage, industrial pollutants, fer... more The quality of groundwater has been degrading due to municipal sewage, industrial pollutants, fertilizers, herbicides, and pesticides. These dangerous pollutants enter into the deeper soil layers, infiltrate some aquifers, and decrease the gradation of groundwater. Other problems are associated with the leakage of sewer, faulty septic tank cleaning, landfill leachates, throwing of garbage into the river, pond and soil pollution. In coastal areas, salt water intrudes into freshwater aquifers due to intensive pumping of fresh groundwater. In the present study, the city Kashipur in the state of Uttarakhand has been chosen due to big industrial settlements. The industrial wastes contain many highly harmful elements which destroy the quality of groundwater in the adjacent areas. Therefore, it is our target to test the groundwater quality of Kashipur industrial areas. To analyze the groundwater and to assess the impact of groundwater pollution of Kashipur area, an attempt has been made in the present study to test the physicochemical parameters including pH, total hardness, alkalinity, chloride, fluoride, sulphate, iron, zinc, copper and heavy metal atoms such as lead, arsenic etc. Imbalance of these parameters may degrade the quality of groundwater and may be deleterious to the health of individual and society in context of drinking, agriculture and industrial purposes. Physiochemical treatment of groundwater samples collected in summer, rainy season and post-monsoon were compared and analyzed by regression modeling making a quantitative correlation between pH and other parameters including total hardness, alkalinity, chloride, sulfate, fluoride and copper using multiple linear regression methods.
The Reliability Prediction is an important tool for designing, decision making and estimating fut... more The Reliability Prediction is an important tool for designing, decision making and estimating future system success. Design engineers are often required to develop and estimate Reliability before the product is produced. Inaccurate predictions can lead to over design and/or excessive spare parts procurement. This work is based on the study of Reliability Analysis carried out on Electronic Communication Systems used in the aircraft avionics. This system was applied in the beginning for the Secure Speech Equipment designed specifically to encrypt voices as well as for fax and computer data. The Part Stress Analysis modeling is used in this study which is a worldwide standard for performing reliability predictions. The Reliability Block diagram is also developed as a tool for reliability prediction.
Supplemental material, Supplementary_Information_of_scoparia_revised_updated for Isolation and ch... more Supplemental material, Supplementary_Information_of_scoparia_revised_updated for Isolation and characterization of cytotoxic and anti-inflammatory constituents from Scoparia dulcis L by Mohammad Nur-e-Alam, Sarfaraz Ahmed, Muhammad Yousaf, Shabana I Khan, Ramzi A Mothana and Adnan J Al-Rehaily in Journal of Chemical Research
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Journal of Evolution of Medical and Dental Sciences, Mar 25, 2013
Extended spectrum beta lactamase (ESBL) producing Klebsiella spp have emerged as an important pat... more Extended spectrum beta lactamase (ESBL) producing Klebsiella spp have emerged as an important pathogen due to their high resistance against most of the antibiotics. Their detection and antibiotic resistance pattern is required for proper management of cases. In this study we report the prevalence and antibiotic resistance of such Klebsiella isolates. METHODS: A total of 100 clinical isolates of Klebsiella from different clinical samples were tested for ESBL production by double disc approximation test and CLSI phenotypic method. Antibiotic susceptibility of all the Klebsiella isolates were performed by Kirby-Bauer disc diffusion test. RESULTS: of 100 Klebsiella isolates 53 showed ESBL production. All ESBL producers were resistant to beta lactam antibiotics. Antibiotic resistance among ESBL producing strains was high as compared to non ESBL producing strains. INTERPRETATION & CONCLUSION: In the present study a large number of Klebsiella spp. isolated were found to be ESBL producers. Continuing monitoring of ESBL production and antimicrobial susceptibility testing is essential to avoid treatment failure.
2018 IEEE International Workshop on Signal Processing Systems (SiPS), 2018
Here, we propose a low cost, energy efficient embedded solution for the management of solid wasta... more Here, we propose a low cost, energy efficient embedded solution for the management of solid wastage. The core of this solution is a portable device consisting of an ultrasonic sensor, a processor (NodeMCU) with integrated transmitter for communicating to cloud server (firebase) and a power source (battery). The device comes with most of the information (e.g. unique identity) preprogrammed into its processor meaning the end user will need little to no manual interventions. To increase battery life, intelligent wake-up system has been implemented into the device that significantly reduces the power consumption of the system. The proposed solution will be beneficial to authority responsible for cleaning as they can now track the place where they should go and clean first. Municipalities can also get the statistical information about the amount of garbage produced in the city.
Increasing prevalence of methicillin resistant S. aureus (MRSA) and resistanceto multiple antibio... more Increasing prevalence of methicillin resistant S. aureus (MRSA) and resistanceto multiple antibiotic classes is a global issue. Regional surveillance of antibiotic susceptibility of the organism is a necessary step to overcome the issues of antimicrobial resistance and treatment failure in MRSA infections. The study was conducted to find the pattern of antibiotic susceptibility in MRSA isolated from the pus samples of patients attending a tertiary care hospital in Bastar tribal region in Central India. The study was performed on 215 MRSA isolates cultured from pus samples of patients over a period of two years and five months. In the methicillin resistant organisms selected by oxacillin screen agar test and cefoxitin disk diffusion test, antibiotic susceptibility was determined by Kirby Baur disk diffusion test with CLSI guide lines. Of the total S. aureus isolates, the incidence of MRSA was 34.1% of which 82.8% were resistant to coâ€trimoxazole, 77.2% to tetracycline, 68.8% to gen...
Abstract An orthopedic implant is a medical device used for treating the disability caused by var... more Abstract An orthopedic implant is a medical device used for treating the disability caused by various bone trauma such as fracture and also used to replace a missing joint or to provide support to the affected bone. The orthopedic implants have evolved through various generations. The recent developments in implants are focused on tissue-implant responses and the ways to answer them. The present chapter gives a comprehensive overview of the implants with respect to their history, materials used in the manufacture of implants, and associated properties of the materials. An emphasis is also laid on the complications associated with the implantation and methods to get past them both by tweaking the properties (increasing the biocompatibility) and manufacturing method. The required properties of the materials are also discussed in detail along with their evolution from metals to ceramics. Fabrication techniques including electrostatic spray deposition (ESD) and fiber laser surface engineering are also discussed with a proper focus on the entire design process involving verification, validation, and transfer. Currently, for the manufacture of orthopedic implants, directives of different countries, for example, European Union, United States, and Japan, are being followed. This chapter provides an overview of various implant materials used in orthopedics, various generations, and the vital properties critical for the selection of implant materials. This chapter also gives an idea about the design and development and manufacturing of orthopedic implants. The complications associated with the performance of the implant materials are also addressed to a sufficient extent in this chapter along with regulatory approval requirements, clinical trials, and current market status.
In the present study the dried material of Vernonia schimperi (Syn. Vernonia abyssinica Sch. Bip.... more In the present study the dried material of Vernonia schimperi (Syn. Vernonia abyssinica Sch. Bip. ex Walp.) was successively extracted with acetone and 96% ethanol. These extracts were further fractionated with different solvents and were analyzed for their phytoconstituents using standard protocols. From the study it was found that alkaloids and terpenoids are totally absent while the test for phenols found positive in all the fractions. Hexane fractions as well as acetonitrile fraction of one minute acetone extract showed the presence of glycosides. The sterols are only present in hexane fraction of one minute acetone extract. Most of the constituents are present in polar solvent fractions. Over all plant is rich in pharmaceutically important phytochemicals.
2018 IEEE/ACIS 17th International Conference on Computer and Information Science (ICIS), 2018
Bangladesh suffers more for being susceptible to flood driven loses than relishing the natural ad... more Bangladesh suffers more for being susceptible to flood driven loses than relishing the natural advantages of being a riverine country. It is crucial for flood management department to identify the severity of flood and the factors behind it. Though three main contributors to flood occurrences are the Brahmaputra, Ganges and Meghna river basins, there are several factors such as rainfall, location, elevation and many more that also has prominent influences in causing flood in different regions of Bangladesh. This paper intends to identify the flood condition of different regions of Bangladesh by implementing different clustering techniques such as K-means, Expectation Maximization and Agglomerative Hierarchical clustering algorithm. This paper aims not only to analyze the factors that lead to flood condition but also observe the effectiveness of applying the chosen clustering methods for further analysis.
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Papers by Sarfaraz Ahmed