HAL (Le Centre pour la Communication Scientifique Directe), May 16, 2017
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution-ShareAlike| 4.0 International License
International Journal of Molecular Sciences, Apr 15, 2019
Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in ... more Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 • C, retaining 100% activity after 3 h at 50 • C. Both enzymes were stable between pH 4-6. Different substrate specificities were exhibited, and the lowest K m and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes-Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5-were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms.
Background: 5-Hydroxymethylfurfural (HMF), a major residual component of a lignocellulosic bio-re... more Background: 5-Hydroxymethylfurfural (HMF), a major residual component of a lignocellulosic bio-refinery process, can be transformed into fundamental building blocks for green chemistry via oxidation. While chemical methods are well established, interest is also being directed into the enzymatic oxidation of HMF into the bio-plastic precursor 2,5-furandicarboxylic acid (FDCA). Results: We demonstrate that three glyoxal oxidases (PciGLOX) isoenzymes from the Basidiomycete fungus Pycnoporus cinnabarinus were able to oxidize HMF, with PciGLOX2 and PciGLOX3 being the most efficient. The major reaction product obtained with the three isoenzymes was 5-hydroxymethyl-2-furancarboxylic (HMFCA), a precursor in polyesters and pharmaceuticals production, and very little subsequent conversion of this compound was observed. However, small concentrations of FDCA, a substitute for terephthalic acid in the production of polyesters, were also obtained. The oxidation of HMF was significantly boosted in the presence of catalase for PciGLOX2, leading to 70% HMFCA yield. The highest conversion percentages were observed on 2,5-furandicarboxaldehyde (DFF), a minor product from the reaction of PciGLOX on HMF. To bypass HMFCA accumulation and exploit the efficiency of PciGLOX in oxidizing DFF and 5-formyl-2-furan carboxylic acid (FFCA) towards FDCA production, HMF was oxidized in a cascade reaction with an aryl alcohol oxidase (UmaAAO). After 2 h of reaction, UmaAAO completely oxidized HMF to DFF and further to FFCA, with FDCA only being detected when PciGLOX3 was added to the reaction. The maximum yield of 16% FDCA was obtained 24 h after the addition of PciGLOX3 in the presence of catalase. Conclusions: At least two conversion pathways for HMF oxidation can be considered for PciGLOX; however, the highest selectivity was seen towards the production of the valuable polyester precursor HMFCA. The three isoenzymes showed differences in their catalytic efficiencies and substrate specificities when reacted with HMF derivatives.
Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Al... more Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Although some Phyllosticta species are known to cause plant diseases, ...
Camarosporium is a large coelomycetous genus which was formerly recognised as an asexual state in... more Camarosporium is a large coelomycetous genus which was formerly recognised as an asexual state in Botryosphaeriales and Cucurbitariaceae. In the present study, we collected several Camarosporium-like taxa in Europe (Germany and Italy) and carried out morpho-molecular analyses. Molecular analyses (maximum likelihood, maximum parsimony and MrBayes) of combined LSU and SSU gene datasets show that the Camarosporium-like taxa are polyphyletic in Pleosporales. Camarosporium quaternatum, the type species of Camarosporium clusters in the suborder Pleosporinae with five other Camarosporium species. This clade is supported by high bootstrap and PP values and is distinct from other well-established families in Pleosporinae. Other Camarosporium-like taxa grouped in Montagnulaceae (Massarineae) as two phylogenetically distinct clades and are introduced as two new genera, viz. Paracamarosporium and Pseudocamarosporium. Paracamarosporium is morphologically distinct as it has paraphyses and microconidia, while Pseudocamarosporium lacks both of these characters. Since Camarosporium comprises a large number of species epithets, recollection and morpho-molecular studies of other Camarosporium-like taxa is essential. Coelomycetes / Massarineae / Multi-gene analyses / Phylogeny / Pleosporinae
HAL (Le Centre pour la Communication Scientifique Directe), 2017
The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class o... more The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class of fungi whose members span a wide spectrum of lifestyles and host interactions. The endophytic fungus Phyllosticta is one members of the Dothideomycetes, causing disease in economic crops. Phyllosticta was screened for the degradation of lignocellulosic biomass of commercial relevance, such as rice straw, rice husk, sorghum, wheat straw, miscanthus, lavender flower, and lavender straw. The highest degrading strains were identified from an initial screen and further analyzed for the secretion of lignocellulosic enzymes during growth on the different biomasses. With Phyllosticta capitalensis (MFLUCC14-0233), maximum activity of arabinase (944.18 U/ml culture), cellulase (27.10 U/ml), xylanase (10.85 U/ml), pectinase (465.47 U/ml), and laccase (35.68 U/ml) activities could be detected in the secretome during growth on lavender flowers and lavender straw. Phyllosticta capitalensis is thus an interesting new strain for the production of lignocellulosic enzymes during growth on cheap agro-industrial biomass.
La biomasse vegetale est une alternative durable et ecologique pour les ressources fossiles. L... more La biomasse vegetale est une alternative durable et ecologique pour les ressources fossiles. L'exploitation et la valorisation de cette biomasse sont rendues possibles grâce a la capacite naturelle des enzymes fongiques a degrader et modifier cette biomasse. Parmi ces enzymes, les glyoxal oxydases generatrices de H2O2 (GLOX) restent un groupe peu etudie avec un seul exemple de proteine caracterisee dans la litterature a partir d’un champignon degradant le bois.Dans cette these, trois GLOX, precedemment identifiees dans le genome du champignon degradant le bois Pycnoporus cinnabarinus (PciGLOX), ont ete selectionnees, produites par voie heterologue et caracterisees. La caracterisation a revele des differences entre les trois PciGLOX dans la stabilite des proteines, la specificite du substrat et l’efficacite catalytique. Les proteines PciGLOX sont produites sous leur forme inactive et leur mecanisme d'activation a ete etudie. La capacite des GLOX a catalyser la reaction d'...
FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of... more FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of the fungus. B. Conidiomata (arrows) on the host surface. C. L.S. of a conidioma. D–F. L.S. of a conidioma wall. G–I. Conidigenous cells with developing conidia. J–L. Conidiogenous cells with developing conidia stained with lactophenol cotton blue. M–O. Conidia. P. Conidia stained with lactophenol cotton blue. Q. Germinating conidium. R–S. Colonies on PDA (R. From top, S. From reverse). Scale bars: C = 200 μm; D = 100 μm; E–F = 50 μm; G–Q = 10 μm.
FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum a... more FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum artocarpi.
FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequen... more FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequence data. Parsimony analysis bootstrap support values (above 70) are given at the nodes. Thickened lines indicate MP of 100. Tremella mesenterica was used as outgroup. Ex-epitype sequences generated in this study are in bold.
FIGURE 3. Chaetospermum artocarpi: A. Dead leaf with conidiomata. B. Conidiomata (arrows) on the ... more FIGURE 3. Chaetospermum artocarpi: A. Dead leaf with conidiomata. B. Conidiomata (arrows) on the host surface. C. L.S. of a conidioma. D–F. L.S. of a conidioma wall. G. L.S. of a conidioma wall stained with lactophenol cotton blue. H–I. Conidiogenous cells with developing conidia stained with lactophenol cotton blue. J–M. Conidia. N–P. Conidia stained with lactophenol cotton blue. Q. Germinating conidium. R–S. Colonies on V8 (R. From top, S. From reverse). Scale bars: C, E = 100 μm; D, F–G = 50 μm; H–Q = 10 μm.
Additional file 2. HPLC chromatogram of the reactions of PciGLOX and UmaAAO on HMF. HPLC chromato... more Additional file 2. HPLC chromatogram of the reactions of PciGLOX and UmaAAO on HMF. HPLC chromatogram of the reactions of (a) PciGLOX1 (red), PciGLOX2 (green) and PciGLOX3 (pink) enzymes on HMF after 24 h of incubation compared to the control (blue) and (b) UmaAAO on HMF at t0 (blue) and after 1 h (red), 2 h (green), 4 h (pink) and 24 h (grey) of reaction.
Additional file 1. Stability of HRP under the reaction conditions. Residual activity of HRP on AB... more Additional file 1. Stability of HRP under the reaction conditions. Residual activity of HRP on ABTS after incubation for different time periods (0–24 h) in tartrate buffer pH 6 at 30 °C and 800 rpm (back) and in the presence of 3 mM HMF (blue) or HMFCA (green).
Non-commercial: <RXPD\QRWXVHWKLVZRUNIRUFRPPHUFLDOSXUSRVHV No derivative works: <RXPD\QRWDOW... more Non-commercial: <RXPD\QRWXVHWKLVZRUNIRUFRPPHUFLDOSXUSRVHV No derivative works: <RXPD\QRWDOWHUWUDQVIRUPRUEXLOGXSRQWKLVZRUN For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at
The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class o... more The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class of fungi whose members span a wide spectrum of lifestyles and host interactions. The endophytic fungus Phyllosticta is one members of the Dothideomycetes, causing disease in economic crops. Phyllosticta was screened for the degradation of lignocellulosic biomass of commercial relevance, such as rice straw, rice husk, sorghum, wheat straw, miscanthus, lavender flower, and lavender straw. The highest degrading strains were identified from an initial screen and further analyzed for the secretion of lignocellulosic enzymes during growth on the different biomasses. With Phyllosticta capitalensis (MFLUCC14-0233), maximum activity of arabinase (944.18 U/ml culture), cellulase (27.10 U/ml), xylanase (10.85 U/ml), pectinase (465.47 U/ml), and laccase (35.68 U/ml) activities could be detected in the secretome during growth on lavender flowers and lavender straw. Phyllosticta capitalensis is thus an interesting new strain for the production of lignocellulosic enzymes during growth on cheap agro-industrial biomass.
Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Al... more Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Although some Phyllosticta species are known to cause plant diseases, ...
Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in ... more Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 °C, retaining 100% activity after 3 h at 50 °C. Both enzymes were stable between pH 4-6. Different substrate specificities were exhibited, and the lowest Km and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes-Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5-were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms.
FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of... more FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of the fungus. B. Conidiomata (arrows) on the host surface. C. L.S. of a conidioma. D–F. L.S. of a conidioma wall. G–I. Conidigenous cells with developing conidia. J–L. Conidiogenous cells with developing conidia stained with lactophenol cotton blue. M–O. Conidia. P. Conidia stained with lactophenol cotton blue. Q. Germinating conidium. R–S. Colonies on PDA (R. From top, S. From reverse). Scale bars: C = 200 μm; D = 100 μm; E–F = 50 μm; G–Q = 10 μm.
FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum a... more FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum artocarpi.
FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequen... more FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequence data. Parsimony analysis bootstrap support values (above 70) are given at the nodes. Thickened lines indicate MP of 100. Tremella mesenterica was used as outgroup. Ex-epitype sequences generated in this study are in bold.
HAL (Le Centre pour la Communication Scientifique Directe), May 16, 2017
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Distributed under a Creative Commons Attribution-ShareAlike| 4.0 International License
International Journal of Molecular Sciences, Apr 15, 2019
Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in ... more Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 • C, retaining 100% activity after 3 h at 50 • C. Both enzymes were stable between pH 4-6. Different substrate specificities were exhibited, and the lowest K m and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes-Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5-were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms.
Background: 5-Hydroxymethylfurfural (HMF), a major residual component of a lignocellulosic bio-re... more Background: 5-Hydroxymethylfurfural (HMF), a major residual component of a lignocellulosic bio-refinery process, can be transformed into fundamental building blocks for green chemistry via oxidation. While chemical methods are well established, interest is also being directed into the enzymatic oxidation of HMF into the bio-plastic precursor 2,5-furandicarboxylic acid (FDCA). Results: We demonstrate that three glyoxal oxidases (PciGLOX) isoenzymes from the Basidiomycete fungus Pycnoporus cinnabarinus were able to oxidize HMF, with PciGLOX2 and PciGLOX3 being the most efficient. The major reaction product obtained with the three isoenzymes was 5-hydroxymethyl-2-furancarboxylic (HMFCA), a precursor in polyesters and pharmaceuticals production, and very little subsequent conversion of this compound was observed. However, small concentrations of FDCA, a substitute for terephthalic acid in the production of polyesters, were also obtained. The oxidation of HMF was significantly boosted in the presence of catalase for PciGLOX2, leading to 70% HMFCA yield. The highest conversion percentages were observed on 2,5-furandicarboxaldehyde (DFF), a minor product from the reaction of PciGLOX on HMF. To bypass HMFCA accumulation and exploit the efficiency of PciGLOX in oxidizing DFF and 5-formyl-2-furan carboxylic acid (FFCA) towards FDCA production, HMF was oxidized in a cascade reaction with an aryl alcohol oxidase (UmaAAO). After 2 h of reaction, UmaAAO completely oxidized HMF to DFF and further to FFCA, with FDCA only being detected when PciGLOX3 was added to the reaction. The maximum yield of 16% FDCA was obtained 24 h after the addition of PciGLOX3 in the presence of catalase. Conclusions: At least two conversion pathways for HMF oxidation can be considered for PciGLOX; however, the highest selectivity was seen towards the production of the valuable polyester precursor HMFCA. The three isoenzymes showed differences in their catalytic efficiencies and substrate specificities when reacted with HMF derivatives.
Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Al... more Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Although some Phyllosticta species are known to cause plant diseases, ...
Camarosporium is a large coelomycetous genus which was formerly recognised as an asexual state in... more Camarosporium is a large coelomycetous genus which was formerly recognised as an asexual state in Botryosphaeriales and Cucurbitariaceae. In the present study, we collected several Camarosporium-like taxa in Europe (Germany and Italy) and carried out morpho-molecular analyses. Molecular analyses (maximum likelihood, maximum parsimony and MrBayes) of combined LSU and SSU gene datasets show that the Camarosporium-like taxa are polyphyletic in Pleosporales. Camarosporium quaternatum, the type species of Camarosporium clusters in the suborder Pleosporinae with five other Camarosporium species. This clade is supported by high bootstrap and PP values and is distinct from other well-established families in Pleosporinae. Other Camarosporium-like taxa grouped in Montagnulaceae (Massarineae) as two phylogenetically distinct clades and are introduced as two new genera, viz. Paracamarosporium and Pseudocamarosporium. Paracamarosporium is morphologically distinct as it has paraphyses and microconidia, while Pseudocamarosporium lacks both of these characters. Since Camarosporium comprises a large number of species epithets, recollection and morpho-molecular studies of other Camarosporium-like taxa is essential. Coelomycetes / Massarineae / Multi-gene analyses / Phylogeny / Pleosporinae
HAL (Le Centre pour la Communication Scientifique Directe), 2017
The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class o... more The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class of fungi whose members span a wide spectrum of lifestyles and host interactions. The endophytic fungus Phyllosticta is one members of the Dothideomycetes, causing disease in economic crops. Phyllosticta was screened for the degradation of lignocellulosic biomass of commercial relevance, such as rice straw, rice husk, sorghum, wheat straw, miscanthus, lavender flower, and lavender straw. The highest degrading strains were identified from an initial screen and further analyzed for the secretion of lignocellulosic enzymes during growth on the different biomasses. With Phyllosticta capitalensis (MFLUCC14-0233), maximum activity of arabinase (944.18 U/ml culture), cellulase (27.10 U/ml), xylanase (10.85 U/ml), pectinase (465.47 U/ml), and laccase (35.68 U/ml) activities could be detected in the secretome during growth on lavender flowers and lavender straw. Phyllosticta capitalensis is thus an interesting new strain for the production of lignocellulosic enzymes during growth on cheap agro-industrial biomass.
La biomasse vegetale est une alternative durable et ecologique pour les ressources fossiles. L... more La biomasse vegetale est une alternative durable et ecologique pour les ressources fossiles. L'exploitation et la valorisation de cette biomasse sont rendues possibles grâce a la capacite naturelle des enzymes fongiques a degrader et modifier cette biomasse. Parmi ces enzymes, les glyoxal oxydases generatrices de H2O2 (GLOX) restent un groupe peu etudie avec un seul exemple de proteine caracterisee dans la litterature a partir d’un champignon degradant le bois.Dans cette these, trois GLOX, precedemment identifiees dans le genome du champignon degradant le bois Pycnoporus cinnabarinus (PciGLOX), ont ete selectionnees, produites par voie heterologue et caracterisees. La caracterisation a revele des differences entre les trois PciGLOX dans la stabilite des proteines, la specificite du substrat et l’efficacite catalytique. Les proteines PciGLOX sont produites sous leur forme inactive et leur mecanisme d'activation a ete etudie. La capacite des GLOX a catalyser la reaction d'...
FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of... more FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of the fungus. B. Conidiomata (arrows) on the host surface. C. L.S. of a conidioma. D–F. L.S. of a conidioma wall. G–I. Conidigenous cells with developing conidia. J–L. Conidiogenous cells with developing conidia stained with lactophenol cotton blue. M–O. Conidia. P. Conidia stained with lactophenol cotton blue. Q. Germinating conidium. R–S. Colonies on PDA (R. From top, S. From reverse). Scale bars: C = 200 μm; D = 100 μm; E–F = 50 μm; G–Q = 10 μm.
FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum a... more FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum artocarpi.
FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequen... more FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequence data. Parsimony analysis bootstrap support values (above 70) are given at the nodes. Thickened lines indicate MP of 100. Tremella mesenterica was used as outgroup. Ex-epitype sequences generated in this study are in bold.
FIGURE 3. Chaetospermum artocarpi: A. Dead leaf with conidiomata. B. Conidiomata (arrows) on the ... more FIGURE 3. Chaetospermum artocarpi: A. Dead leaf with conidiomata. B. Conidiomata (arrows) on the host surface. C. L.S. of a conidioma. D–F. L.S. of a conidioma wall. G. L.S. of a conidioma wall stained with lactophenol cotton blue. H–I. Conidiogenous cells with developing conidia stained with lactophenol cotton blue. J–M. Conidia. N–P. Conidia stained with lactophenol cotton blue. Q. Germinating conidium. R–S. Colonies on V8 (R. From top, S. From reverse). Scale bars: C, E = 100 μm; D, F–G = 50 μm; H–Q = 10 μm.
Additional file 2. HPLC chromatogram of the reactions of PciGLOX and UmaAAO on HMF. HPLC chromato... more Additional file 2. HPLC chromatogram of the reactions of PciGLOX and UmaAAO on HMF. HPLC chromatogram of the reactions of (a) PciGLOX1 (red), PciGLOX2 (green) and PciGLOX3 (pink) enzymes on HMF after 24 h of incubation compared to the control (blue) and (b) UmaAAO on HMF at t0 (blue) and after 1 h (red), 2 h (green), 4 h (pink) and 24 h (grey) of reaction.
Additional file 1. Stability of HRP under the reaction conditions. Residual activity of HRP on AB... more Additional file 1. Stability of HRP under the reaction conditions. Residual activity of HRP on ABTS after incubation for different time periods (0–24 h) in tartrate buffer pH 6 at 30 °C and 800 rpm (back) and in the presence of 3 mM HMF (blue) or HMFCA (green).
Non-commercial: <RXPD\QRWXVHWKLVZRUNIRUFRPPHUFLDOSXUSRVHV No derivative works: <RXPD\QRWDOW... more Non-commercial: <RXPD\QRWXVHWKLVZRUNIRUFRPPHUFLDOSXUSRVHV No derivative works: <RXPD\QRWDOWHUWUDQVIRUPRUEXLOGXSRQWKLVZRUN For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at
The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class o... more The Dothideomycetes represent the largest fungal class of Ascomycota. It is an ubiquitous class of fungi whose members span a wide spectrum of lifestyles and host interactions. The endophytic fungus Phyllosticta is one members of the Dothideomycetes, causing disease in economic crops. Phyllosticta was screened for the degradation of lignocellulosic biomass of commercial relevance, such as rice straw, rice husk, sorghum, wheat straw, miscanthus, lavender flower, and lavender straw. The highest degrading strains were identified from an initial screen and further analyzed for the secretion of lignocellulosic enzymes during growth on the different biomasses. With Phyllosticta capitalensis (MFLUCC14-0233), maximum activity of arabinase (944.18 U/ml culture), cellulase (27.10 U/ml), xylanase (10.85 U/ml), pectinase (465.47 U/ml), and laccase (35.68 U/ml) activities could be detected in the secretome during growth on lavender flowers and lavender straw. Phyllosticta capitalensis is thus an interesting new strain for the production of lignocellulosic enzymes during growth on cheap agro-industrial biomass.
Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Al... more Resumen en: Phyllosticta fungi are widely distributed and appear to have different lifestyles. Although some Phyllosticta species are known to cause plant diseases, ...
Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in ... more Two laccase-encoding genes from the marine-derived fungus Pestalotiopsis sp. have been cloned in Aspergillus niger for heterologous production, and the recombinant enzymes have been characterized to study their physicochemical properties, their ability to decolorize textile dyes for potential biotechnological applications, and their activity in the presence of sea salt. The optimal pH and temperature of PsLac1 and PsLac2 differed in relation to the substrates tested, and both enzymes were shown to be extremely stable at temperatures up to 50 °C, retaining 100% activity after 3 h at 50 °C. Both enzymes were stable between pH 4-6. Different substrate specificities were exhibited, and the lowest Km and highest catalytic efficiency values were obtained against syringaldazine and 2,6-dimethoxyphenol (DMP) for PsLac1 and PsLac2, respectively. The industrially important dyes-Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5-were more efficiently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). Activities were compared in saline conditions, and PsLac2 seemed more adapted to the presence of sea salt than PsLac1. The overall surface charges of the predicted PsLac three-dimensional models showed large negatively charged surfaces for PsLac2, as found in proteins for marine organisms, and more balanced solvent exposed charges for PsLac1, as seen in proteins from terrestrial organisms.
FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of... more FIGURE 2. Chaetospermum camelliae: A. Dead leaf with conidiomata, arrow indicates the position of the fungus. B. Conidiomata (arrows) on the host surface. C. L.S. of a conidioma. D–F. L.S. of a conidioma wall. G–I. Conidigenous cells with developing conidia. J–L. Conidiogenous cells with developing conidia stained with lactophenol cotton blue. M–O. Conidia. P. Conidia stained with lactophenol cotton blue. Q. Germinating conidium. R–S. Colonies on PDA (R. From top, S. From reverse). Scale bars: C = 200 μm; D = 100 μm; E–F = 50 μm; G–Q = 10 μm.
FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum a... more FIGURE 4. Conidia with appendages and conidiophore. a. Chaetospermum camelliae b. Chaetospermum artocarpi.
FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequen... more FIGURE 1. Maximum parsimonious tree of Chaetospermum and related genera generated from LSU sequence data. Parsimony analysis bootstrap support values (above 70) are given at the nodes. Thickened lines indicate MP of 100. Tremella mesenterica was used as outgroup. Ex-epitype sequences generated in this study are in bold.
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