This work presents the findings of different disciplines, methodologies and fields of action of s... more This work presents the findings of different disciplines, methodologies and fields of action of social scientists, in which an intra and extra-disciplinary dialogue is established on coexistence and prosociality. For this reason, the compilation includes complementary references in methods, experiments, correlations, studies of social networks, etc.; concepts, verbiage, executive functions, environmental conditions, cultural aspects; and units of analysis, for example, individual, culture, groups, texts, etc. The chapters present research results carried out in specialized settings both at university and in socially situated contexts. This book is in line with the scientific agenda and the national current challenges such as the educational impacts of the conflict as regards the post-peace agreement with the FARC-EP; the group dynamics of the groups of victims; the conflict associated with urban life, the physical violence against women; the fulfillment of education goals for sustai...
Recent studies have identified several key intermediates of the nitric oxide reductase (NOR) cycl... more Recent studies have identified several key intermediates of the nitric oxide reductase (NOR) cycle of flavin-diiron proteins (FDPs). These intermediates include, sequentially, a -hydroxo diferrous species (FDPred), a mono-NO intermediate (FDPNO), a di-NO intermediate (FDPdiNO), and a bis-hydroxo diferric (FDPox) species. The paper focuses on the reaction path connecting the last two intermediates on which the nitrous oxide product is released. Based on DFT calculations a reaction sequence is proposed in which the enzyme passes through an intermediate with a bridging hyponitrite ligand, which accepts a proton, initiating a rate-determining ligand rotation from an N/N-to an N/O-coordinated conformation. This rotation is facilitated by a second sphere tyrosine residue, which provides a transient hydrogen bond to one of the nitrogen atoms of the substrate near the transition state. The role of the tyrosine residue for the NOR activity has been tested by steady-state kinetics and rapid freeze quench (RFQ) studies of the Y197F variant of the FDP from T. maritima in which the hydrogen bonding interaction is absent. The Y197F variant displayed little or no steady-state NOR activity in support of the importance of Y197. The RFQ samples, monitored with Mössbauer spectroscopy, showed that Y197F follows the same reaction path as wild-type up to and including the formation of FDPdiNO but diverges subsequently with the variant forming an inactive mono-NO species. The RFQ results demonstrate that Y197 enables the post FDPdiNO section of the reaction cycle in wild-type to proceed to N2O. The proposed refinement of the reaction mechanism provides an explanation for the lack of NOR activity of the variant Y197F of T. maritima and of other di-NO binding diiron enzymes and model compounds with active site structures like those of FDP. The reported reductions of NO to N2O catalyzed by synthetic diiron complexes proceed only with the support of radiation, additional electrons, or an electron-rich ligand environment. However, higher potential diiron sites like those of FDP require hydrogen bonding to second sphere residues to turn over NO to N2O.
Alumnos del docente Javier Asdrubal Vinasco G Concierto Auditorio MAJA Febrero 9 2019 Hora: 7:00 ... more Alumnos del docente Javier Asdrubal Vinasco G Concierto Auditorio MAJA Febrero 9 2019 Hora: 7:00 p.m. Coordinador Jorge Alberto Gaviria
En la historia de la humanidad son muchos los ejemplos de individuos que, poniendo en riesgo su p... more En la historia de la humanidad son muchos los ejemplos de individuos que, poniendo en riesgo su propio bienestar, actúan en beneficio de otros. Estas conductas en las que las personas actúan para satisfacer las necesidades de los demás, muchas veces en perjuicio de sí mismas, generan un gran interés en la comunidad científica. Diversos estudios describen estas conductas, denominadas prosociales, como un fenómeno complejo que involucra acciones individuales basadas en creencias y sentimientos(Auné, Blum, Lozzia y Horacio, 2014), en el que una persona beneficia a otras de forma voluntaria (Jirsaraie, Ranby & Albeck, 2019). Esto no solo favorece la relación interpersonal y el bienestar del otro, sino que genera afecto positivo en la persona que ejecuta la acción (Richaud y Mesurado 2016). También sirven como una influencia positiva en el desarrollo del individuo y en sus interacciones sociales; mejoran las relaciones de pares entre los niños de corta edad (Taylor et al., 2013); facilit...
This work presents the findings of different disciplines, methodologies and fields of action of s... more This work presents the findings of different disciplines, methodologies and fields of action of social scientists, in which an intra and extra-disciplinary dialogue is established on coexistence and prosociality. For this reason, the compilation includes complementary references in methods, experiments, correlations, studies of social networks, etc.; concepts, verbiage, executive functions, environmental conditions, cultural aspects; and units of analysis, for example, individual, culture, groups, texts, etc. The chapters present research results carried out in specialized settings both at university and in socially situated contexts. This book is in line with the scientific agenda and the national current challenges such as the educational impacts of the conflict as regards the post-peace agreement with the FARC-EP; the group dynamics of the groups of victims; the conflict associated with urban life, the physical violence against women; the fulfillment of education goals for sustai...
Recent studies have identified several key intermediates of the nitric oxide reductase (NOR) cycl... more Recent studies have identified several key intermediates of the nitric oxide reductase (NOR) cycle of flavin-diiron proteins (FDPs). These intermediates include, sequentially, a -hydroxo diferrous species (FDPred), a mono-NO intermediate (FDPNO), a di-NO intermediate (FDPdiNO), and a bis-hydroxo diferric (FDPox) species. The paper focuses on the reaction path connecting the last two intermediates on which the nitrous oxide product is released. Based on DFT calculations a reaction sequence is proposed in which the enzyme passes through an intermediate with a bridging hyponitrite ligand, which accepts a proton, initiating a rate-determining ligand rotation from an N/N-to an N/O-coordinated conformation. This rotation is facilitated by a second sphere tyrosine residue, which provides a transient hydrogen bond to one of the nitrogen atoms of the substrate near the transition state. The role of the tyrosine residue for the NOR activity has been tested by steady-state kinetics and rapid freeze quench (RFQ) studies of the Y197F variant of the FDP from T. maritima in which the hydrogen bonding interaction is absent. The Y197F variant displayed little or no steady-state NOR activity in support of the importance of Y197. The RFQ samples, monitored with Mössbauer spectroscopy, showed that Y197F follows the same reaction path as wild-type up to and including the formation of FDPdiNO but diverges subsequently with the variant forming an inactive mono-NO species. The RFQ results demonstrate that Y197 enables the post FDPdiNO section of the reaction cycle in wild-type to proceed to N2O. The proposed refinement of the reaction mechanism provides an explanation for the lack of NOR activity of the variant Y197F of T. maritima and of other di-NO binding diiron enzymes and model compounds with active site structures like those of FDP. The reported reductions of NO to N2O catalyzed by synthetic diiron complexes proceed only with the support of radiation, additional electrons, or an electron-rich ligand environment. However, higher potential diiron sites like those of FDP require hydrogen bonding to second sphere residues to turn over NO to N2O.
Alumnos del docente Javier Asdrubal Vinasco G Concierto Auditorio MAJA Febrero 9 2019 Hora: 7:00 ... more Alumnos del docente Javier Asdrubal Vinasco G Concierto Auditorio MAJA Febrero 9 2019 Hora: 7:00 p.m. Coordinador Jorge Alberto Gaviria
En la historia de la humanidad son muchos los ejemplos de individuos que, poniendo en riesgo su p... more En la historia de la humanidad son muchos los ejemplos de individuos que, poniendo en riesgo su propio bienestar, actúan en beneficio de otros. Estas conductas en las que las personas actúan para satisfacer las necesidades de los demás, muchas veces en perjuicio de sí mismas, generan un gran interés en la comunidad científica. Diversos estudios describen estas conductas, denominadas prosociales, como un fenómeno complejo que involucra acciones individuales basadas en creencias y sentimientos(Auné, Blum, Lozzia y Horacio, 2014), en el que una persona beneficia a otras de forma voluntaria (Jirsaraie, Ranby & Albeck, 2019). Esto no solo favorece la relación interpersonal y el bienestar del otro, sino que genera afecto positivo en la persona que ejecuta la acción (Richaud y Mesurado 2016). También sirven como una influencia positiva en el desarrollo del individuo y en sus interacciones sociales; mejoran las relaciones de pares entre los niños de corta edad (Taylor et al., 2013); facilit...
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