Papers by Samira Bensoltane
Environmental Research Communications, 2024
Nutrition & santé, Jun 30, 2020
Additifs alimentaires Effets aigus de l'exposition à la tartrazine (E102) sur le comportement et ... more Additifs alimentaires Effets aigus de l'exposition à la tartrazine (E102) sur le comportement et l'histologie d'un modèle biologique l'escargot terrestre Helix aspersa
Journal of entomology and zoology studies, Sep 1, 2016
In the present study, adult snails Helix aspersa were used to estimate the effect of a neonicotin... more In the present study, adult snails Helix aspersa were used to estimate the effect of a neonicotinoid insecticide, thiamethoxam on the acetylcholinesterase (AChE), glutathione S-transferase (GST) and catalase (CAT) activities after a treatment of 6 weeks. During this period, snails were exposed by ingestion and contact to fresh lettuce leaves which were soaked with an insecticide solution. The thiamethoxam test solutions were 0, 25, 50, 100 and 200 mg/L, which are lower or equal to the concentrations that are applied in field. AChE activity was concentration-dependently inhibited by thiamethoxam. Also, GST and CAT activities were induced by neonicotinoïde insecticide with a dosedependent manner. The responses of these enzymes are probably related to an increase in the release of reactive oxygen species in the presence of thiamethoxam. These results suggest the neurotoxic effect of thiamethoxam on snails exposed to the lettuce contaminated with this insecticide. In same time, this compound has effects on the digestive system, in addition to this neurotoxic effect in Helix aspersa (nontarget organism).
AGROBIOLOGIA, Dec 30, 2019
Sciences & Technologie. C, Biotechnologies, Jun 1, 2011
Le stress oxydant qui résulte, rappelons-le, d'un déséquilibre de la balance entre pro-oxydants e... more Le stress oxydant qui résulte, rappelons-le, d'un déséquilibre de la balance entre pro-oxydants et anti-oxydants, peut être évalué selon deux approches principales faisant appel à la mesure :-des systèmes anti-oxydants (enzymatiques et non enzymatiques),-des dommages créés par l'attaque des radicaux libres sur les principales cibles moléculaires biologiques (lipides, protéines, glucides etc…). La difficulté est de choisir tel ou tel biomarqueur pour apprécier son importance in vitro car on se heurte à des problèmes analytiques de spécificité et de sensibilité. L'analyse exploratoire de données expérimentales utilise fréquemment des outils statistiques. cet article présente l'applicabilité détaillée des réseaux neuronaux artificiels (RNA) et à leur contribution à l'étude de l'évaluation du stress oxydatif induit par le cadmium chez Saccharomyces cerevisiae. Il comporte une description de la méthodologie des RNA et l'avantage de l'intelligence artificielle sur l'analyse statistique actuelle. Mots-clés : Stress oxydatif-Biomarqueur-Malonaldéhyde-Cadmium-Saccharomyces cerevisiae-Réseaux neuronaux artificiels (RNA).
Communications in agricultural and applied biological sciences, 2006
The present work aimed at the study of the effects of Artea and Punch; two systemic fungicides on... more The present work aimed at the study of the effects of Artea and Punch; two systemic fungicides on durum Wheat (Triticum durum L. cv. GTA dur). Seeds were grown in a medium containing respectively 25, 50, 75 and 100 ppm of Artea and Punch under controlled conditions. After measuring root number and length, eight-day-old-root tips were used to determine Catalase (EC 1.11.1.6), Ascorbate-Peroxidase (EC 1.11.1.11) and Guaïacol-Peroxidase (EC 1.11.1.7) enzymatic activities. Root respiratory activity was also determined using a polarographic method (Clark electrode). Fungicide treatment triggered a decrease in root number and length for both fungicides. On the other hand, treatment with Artea and Punch resulted in an enhancement of the respiratory activity and increased antioxidative enzymatic levels in durum wheat roots. Activities of Catalase, Ascorbate-Peroxydase and Guaïacol-Peroxydase increased proportionally and were more meaningful at high concentrations (75 and 100 ppm) compared with controls. Modulations in respiratory metabolism and antioxidant system could probably be the result of Artea/Punch-induced toxicity which could lead to an oxidative stress status. The present study enhances previous works relevant to the toxic effects induced by systemic fungicides on plants.
African Journal of Biotechnology, May 31, 2006
Use of fertilizers like ammonium nitrate (NH 4 NO 3) for agricultural purposes has increasingly c... more Use of fertilizers like ammonium nitrate (NH 4 NO 3) for agricultural purposes has increasingly contaminated the ecosystem with nitrate and/or nitrites. Nitrite is a toxic substance that can cause multiple physiological effects if allowed to build up to high concentrations in animals such as methemoglobinemia. This work is concerned with the study of short term (3 days intoxication) and midterm (over 21 days) NH 4 NO 3 exposure to wistar rats at the dose of 250 mg/Kg. Under these conditions, some hematological and biochemical parameters were affected. Methemoglobinemia, increase in serum nitrates as well as a hepatic cytotoxicity indicated by an increase in bilirubin and transaminases levels were observed.
Communications in agricultural and applied biological sciences, 2005
The inopportune throws out of diverse substances in the atmosphere, constitutes without any doubt... more The inopportune throws out of diverse substances in the atmosphere, constitutes without any doubt the obvious of environmental pollution by man. Among these substances, we are interested in the ammonium nitrate NH4NO3, nitrate fertilizers widely used in farming in our region - Annaba located in the eastern part of Algeria - and manufactured in the same region. In fact, the excessive fertilization, the intensive spreading of animal faeces and the industrial pollution are the accumulation sources of nitrate in vegetables, drilling and the underground waters. The goal of this study is to show the obviousness of a possible toxic effect of NH4NO3 on paramecium an inferior plants resed as biological models. The tests of toxicity were achieved on samples of the area of culture of parameciums. Four concentrations were chosen: 10, 20, 30 and 40 mM, and added before and after appearance of the parameciums in the area. The prepared solutions with the same concentrations of NH4NO3, were used for the dampening of the samples of mosses and lichens. The use of an original technique the polarography allowed us to measure the quantity of oxygen consumed by the organisms in presence of the polluent. The results show that: (i): The treatment by NH4NO3 stimulates the respiratory metabolism of paramecium, (ii): with mosses as well as the measure of the consumption of the oxygen shous the abviousness contrasted with a dampening of respiration but also of the photosynthesis. The perturbation of the respiration and photosynthesis of mousses and lichens can explain the degradation of the plant material and the disappearance of certain species from our ecosystem.
Annals of Biological Research, 2013
This work aims the study of the ability of Phragmites australis roots to accumulate and transfer ... more This work aims the study of the ability of Phragmites australis roots to accumulate and transfer metals such as iron and zinc present in water filled with discharges from industrial sources. We are interested in the movement and distribution of these metals in the roots through a lagoon system designed for vertical flow in the laboratory through two trays where treatment plants Phragmites australis were planted. The results showed the presence of high concentrations of metals such as iron and zinc concentrates in the roots. After purification, the concentrations of these metals are highly decreased, confirming the effectiveness of the system.
DOAJ (DOAJ: Directory of Open Access Journals), Jun 1, 2019
everal studies show that nanoparticles (NPs) can penetrate the body through several routes and ha... more everal studies show that nanoparticles (NPs) can penetrate the body through several routes and have systemic toxic effects on human health. This study aims to provide answers to the understanding of the nature and origin of the biological effects of NPs of metal oxide: Fe2O3 on a bioaccumulator and bioindicator species, the terrestrial snail Helix aspersa. Their effects were studied on snail adults by a laboratory-focused approach, and we evaluated the effect of three doses of these nanoparticles (1, 2 and 3 mg∙g-1 of food) in wheat flour for six weeks; the control group receives only flour alone. At the end of the treatment, we measured the levels of antioxidant enzymatic defences: glutathione (GSH), glutathione-S-transferase (GST), glutathione-peroxydase (GPx), malondialdehyde (MDA), catalase (CAT), and histological sections at the kidney level of each group were performed. Our results reveal triggered oxidative stress and an induction of the detoxification system, with an increase in GSH, GST, GPx and MDA levels with a decrease in catalase activity. Very clear renal tissue damage confirms the results obtained at the beginning dosages.
Fresenius Environmental Bulletin, 2014
African Journal of Biotechnology, Nov 29, 2012
In this study, adult snails, Helix aspersa were used to estimate the effect of aneonicotinoid ins... more In this study, adult snails, Helix aspersa were used to estimate the effect of aneonicotinoid insecticide (thiametoxam) on biochemical parameters and histological changes in the hepatopancreas of this gastropod after a treatment of six weeks. During this period, snails were exposed by ingestion and contact to fresh lettuce leaves which were soaked with an insecticide solution. The thiametoxam test solutions were 0, 25, 50, 100 and 200 mg/L. The results of the biochemical dosages (total carbohydrates, total proteins and total lipids) showed significant decreases at two concentrations (100 and 200 mg/L) of thiametoxam. However, the histological examination of the hepatopancreas of the treated snails showed alterations as a response to all the treatments, and revealed the degeneration of the digestive tubules and the breakdown of the basement membrane in a dose-dependent manner, leading to a severe deterioration of the tissues in the concentration of 200 mg/L thiametoxam. The dosage of carbohydrates, lipids and proteins supported by the study of histological changes on the hepatopancreas of H. aspersa can be considered as potential biomarkers of exposure to thiametoxam.
World Academy of Science, Engineering and Technology, International Journal of Bioengineering and Life Sciences, 2014
In the present study, adult snails Helix aspersa were used to estimate the effect of a neonicotin... more In the present study, adult snails Helix aspersa were used to estimate the effect of a neonicotinoid insecticide, thiamethoxam on the acetylcholinesterase (AChE), glutathione S-transferase (GST) and catalase (CAT) activities after a treatment of 6 weeks. During this period, snails were exposed by ingestion and contact to fresh lettuce leaves which were soaked with an insecticide solution. The thiamethoxam test solutions were 0, 25, 50, 100 and 200 mg/L, which are lower or equal to the concentrations that are applied in field. AChE activity was concentration-dependently inhibited by thiamethoxam. Also, GST and CAT activities were induced by neonicotinoide insecticide with a dosedependent manner. The responses of these enzymes are probably related to an increase in the release of reactive oxygen species in the presence of thiamethoxam. These results suggest the neurotoxic effect of thiamethoxam on snails exposed to the lettuce contaminated with this insecticide. In same time, this com...
The objective of this work is to characterize the adaptive response of yeast (Saccharomyces cerev... more The objective of this work is to characterize the adaptive response of yeast (Saccharomyces cerevisiae), in protein, carbohydrate, enzymatic (CAT) and respiratory against cadmium (Cd) and zinc (Zn) in combined treatments in the presence of calcium and oxidative stress that results. The concentrations of cadmium and/or zinc in the presence or absence of calcium were used (0.1 mM Cd /0.1 mM Ca , 0.1 mM 2+ 2+ Cd /0.1 mM Zn , 0.1 mM Ca /0.1 mM Zn and 0.1 mM Cd /0.1 mM Ca / 0.1 mM Zn). The obtained results 2+ 2+ 2+ 2+ 2+ 2+ 2+ showed that the determination of enzyme activity (catalase) is strongly stimulated particularly treatments combined (mixture of two metals), however, reduced the rate of total protein was observed with combination therapy, it is near 22% for treatments Calcium / Zinc and Cadmium / Calcium / Zinc. Meanwhile, the rate of carbohydrate has been a sharp decrease in cells treated with Cd /Ca / Zn. It is about (83%), it is (34%) for 2+ 2+ 2+ the treatment Cd / Ca while it decreases near (48%) for the treatment Cd /Zn. For treated Ca / Zn and 2+ 2+ 2+ 2+ 2+ 2+ Cd /Ca /Zn inhibition of respiratory activity is observed with an oxygen uptake of about (42%). In 2+ 2+ 2+ conclusion, cadmium exerts oxidative stress led to many alterations of biochemical parameters (enzymatic and respiratory).
Biodiversity Journal
The effects of harmful mixtures on living organisms are of greater importance due to exposure of ... more The effects of harmful mixtures on living organisms are of greater importance due to exposure of these complex mixtures of contaminants in the environment. As nanoparticles (NPs) released can potentially interact with many pollutants, and even other NPs in this study, adult Cornu aspersum (Müller, 1774) (Gastropoda Helicidae) were used to estimate a mixture effect of two NPs "Fe 2 O 3 and ZnO" on the exchange of metabolites (carbohydrates, lipids, and proteins), and some oxidative stress biomarkers: Glutathione (GSH), Glutathione-S-Transferase (GST), Catalase (Cat), and by performing hepatopancreas histological sections of this gastropod after four weeks of treatment. During this period, snails have ingested wheat flour containing the powder of this mixture at doses of 0, 1, 2, and 3 mg/g of wheat flour. The biochemical assays of metabolites reveal disturbances in metabolism by increasing the protein content and decreasing lipid and carbohydrate levels. The decrease of GSH level, GST activity, and Cat activity proves that failure of the detoxification system triggers oxidative stress. The histological study confirms the biochemical results by the tissue lesions, which are very serious, and in a surprisingly dependent dose manner with inflammations, necrosis, hypertrophies, degeneration of the connective tissue, and tubular membranes.
Bulletin de la Société Royale des Sciences de Liège
Dans cette étude nous nous sommes intéressés à l’évaluation de l’impact des nanoparticules de sil... more Dans cette étude nous nous sommes intéressés à l’évaluation de l’impact des nanoparticules de silice fumée (SiO2) sur un organisme bioaccumulateur, bioindicateur de pollution de l’environnement et un indicateur d’état sanitaire des sols, le gastéropode Helix aspersa suite à une exposition via la nourriture. La toxicité des NPs de silice est déterminée chez l'escargot Helix aspersa grâce aux expérimentations réalisées au sein du laboratoire sur des escargots partagés en quatre lots : un lot considéré comme témoin et trois autres traités par des doses croissantes de nanoparticules de silice fumée de 250, 500 et 750 µg/g de nourriture (farine de blé) pendant quatre semaines. Le stress oxydatif a été évalué par le biais d’un suivi de l’activité de certains biomarqueurs biochimiques et enzymatiques : GSH, GST, GPx et CAT dans les tissus hépatopancréatiques et rénaux et un dosage des protéines totales a également été effectué. Les résultats obtenus montrent que la présence des nanopar...
Nutrition & Santé, 2020
Introduction. Tartrazine is an azofood additive widely used in the pharmaceu-cal, cosmetic, food,... more Introduction. Tartrazine is an azofood additive widely used in the pharmaceu-cal, cosmetic, food, and other industries. Objective. To assess the effects of tartrazine on the attitude to move and to eat, and histological changes in digestive, and renal sys-tem, after an acute treatment on a biological model the snail Helix aspersa. Material and Methods. Snails were exposed orally, and by contact to flour contaminated with this dye for 24 hours. Adult snails were divided into two groups of 20 animals each. The control group fed flour alone. The experimental group was administered orally with flour treated with tartrazine (200mg/g flour). Results. The observation of behaviour revealed inhibitory effects of tartrazine on locomotion, and nutrition of snails. Histolo-gical examination of intestines, hepatopancreas, and kidney of treated animals showed altered tissue structures, with advanced degeneration of digestive tubules, connective tissue, and smooth muscle fibers of the walls surrou...
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Papers by Samira Bensoltane