Papers by SUHEYLA TURKYiLMAZ
Proceedings of the Bulgarian Academy of Sciences
Calf diarrhea is an important disease that causes economic losses and E. coli is the primary path... more Calf diarrhea is an important disease that causes economic losses and E. coli is the primary pathogen. In this study, stool samples were collected from a total of 100 calves with diarrhea. As a result of 16S rRNA PCR analysis, it was confirmed that 50 (96.15%) of 52 E. coli isolates were molecularly E. coli. As a result of molecular pathotyping studies of 50 E. coli isolates, the pathotypes of 21 (42.0%) E. coli strains were identified. E. coli (n = 50) isolates were found to be resistant to amoxicillin-clavulanate by 34%, to ciprofloxacin by 38%, and to trimethoprim-sulfamethoxazole by 60%. Different degrees of antimicrobial resistance genes were detected in 26 (52%) E. coli isolates. In conclusion, a data source was provided for the antimicrobial treatment procedure in calf diarrhea caused by E. coli in our region, and the results that could guide future studies were also brought to the literature.
DergiPark (Istanbul University), Jun 1, 2007
Bu çalışmada, broylerlerde Salmonella Enteritidis ve Salmonella Typhimurium infeksiyonlarının Enz... more Bu çalışmada, broylerlerde Salmonella Enteritidis ve Salmonella Typhimurium infeksiyonlarının Enzyme-Linked Immunosorbent Assay (ELISA) ve drag sıvap yöntemleri kullanılarak incelenmesi amaçlandı. Çalışmada 50 kanatlı çiftliğinden 900 kan ve 50 drag sıvap örneği alındı. Alınan serum örneklerinin ELISA ile incelenmesi sonucunda işletmelerin 11'i (% 22,0), serumların ise 96 (% 10,7)'sı pozitif; 39 (% 78,0) işletmeden alınan 804 (% 89,3) serum ise negatif olarak belirlendi. Alınan 50 drag sıvap örneğinin 10 (% 20,0)'undan izolasyon yapıldı. İzole edilen suşlardan 1'i (% 10,0) S. Typhimurium, 8'i (% 80) S. Enteritidis olarak serotiplendirilirken; 1 (% 10,0) suşun ise mevcut antiserumlar ile serotiplendirilmesi yapılamadı. Salmonella infeksiyonlarının tanısında bakteriyolojik ve serolojik çalışmaların birlikte yürütülmesinin daha faydalı olacağı; bununla birlikte Salmonella Gallinarum ve Salmonella Pullorum eradikasyonuna gösterilen önem ve hassasiyetin S. Enteritidis ve S. Typhimurium için de gösterilmesi gerektiği sonucuna varıldı.
Eurasian Journal of Veterinary Sciences, 2017
International Journal of Research in Medical Sciences, Nov 25, 2017
Background: Among the vaginal diseases seen in women, candidiasis is in the first place. This dis... more Background: Among the vaginal diseases seen in women, candidiasis is in the first place. This disease, which is caused by Candida species, can sometimes persist very stubbornly. The aim of this study was to determine Candida species isolated from vaginal specimens by using VITEK MS (MALDI-TOF Microbial Identification System) rapid identification system and to evaluate their susceptibility to some antifungals. Methods: In this study, 220 cervicovaginal swab were used. Isolates were identified by VITEK MS rapid identification system. After identification, antifungal susceptibility testing was performed using the M-44 A2 guideline of The Clinical and Laboratory Standards Institute (CLSI). Results: Total 16.3% (36) of Candida spp. positivity was determined from 220 cervicovaginal samples, and 25 (69.4%) C. glabrata, 6 (16.7%) C. albicans, 3 (8.3%) C. kefyr and 2 (5.6%) C. krusei were obtained with Vitek MS. All identified C. albicans strains were found to be completely resistant to all antifungals used except nystatin agent, C. krusei strains were found to be resistant to flucytosine but sensitive to all other antifungals, C. glabrata and C. kefyr strains were susceptible to all antifungals within the antifungals used in this study. Conclusions: It is concluded that it is necessary to distinguish Candida species in order to apply a correct treatment. And species selection is very important for the selection of antifungal to be used. Nystatin is recommended if no laboratory tests are to be performed for the diagnosis of Vaginal Candidiasis.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2017
Antimicrobial resistant enterococci are among the leading causes of nosocomial infections. Transm... more Antimicrobial resistant enterococci are among the leading causes of nosocomial infections. Transmission of antimicrobial-resistant enterococci from animals to humans has been shown. For this reason, continuous monitoring of antimicrobial resistance in different animal species is of importance both for animal and human health. In this study, it was aimed to investigate the antimicrobial resistance profiles, resistance mechanisms implicated and virulence traits of 107 enterococci isolated from 125 rectal swab samples taken from dogs. The highest resistance rate was determined against tetracycline (65.4%), followed by ciprofloxacin (19.6%), erythromycin (19.6%), chloramphenicol (8.4%) and ampicillin (3.7%). Fourteen (12.1%) enterococci showed multidrug resistance (MDR) phenotype. The tetM gene was predominantly detected among tetracycline isolates. Of 21 erythromycin resistant isolates, 18 harbored the ermB gene. The frequently detected virulence genes was ccf (54.2%), efaAfs (52.3%), cpd (45.8%) and gelE (44.9%). These results indicate that high level of antimicrobial resistance and virulence genes exist among enterococci from dogs and pose a potential public health concern.
Acta Veterinaria-beograd, Sep 1, 2016
In this study, determination of enterococcus species that were isolated from mastitic milk sample... more In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identifi cation of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) Enterococcus spp. were isolated. A total of 94 species of Enterococci were identifi ed using both sequencing and polymerase chain reaction (PCR). Enterococcus spp. isolates belong to 5 different species (E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii) in sequence analysis and 4 different species (E. faecalis, E. faecium, E. durans, E. hirae) were identify by PCR method with specifi c primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The tet resistance genes were identifi ed as follows: 54 were tetM positive, 23 were tetK positive and 17 were positive on tetM and tetK. Resistance to erythromycin was established in 27 (28.7%) isolates (25 ermB) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the cat gene was identifi ed in nine samples and one isolate was resistant to vancomycin (1.06%) with the VanA gene confi rmed. In conclusion, it was shown that E. faecalis has the biggest role in enterococcus originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the VanA gene was also determined.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2022
This study aimed to investigate the presence and distribution of virulence determinants and their... more This study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibilities of 44 Pseudomonas aeruginosa isolates obtained from clinical bovine mastitis cases. In addition, selected 6 P. aeruginosa isolates were further characterized using whole-genome sequencing (WGS). Based on the presence of T3SS-related genes, 25% of the isolates were found to carry exoU and/or exoS genes belonging to invasive (exoU-/exoS+, 18.2%) and cytotoxic (exoU+/exoS-, 6.8%) strains. But, none of the isolates carried both exoU and exoS genes. In terms of other virulence genes examined, various virulence gene profiles were observed among the isolates. The majority of the isolates (72.7%) were susceptible to all tested antimicrobials. Resistance rates to ciprofloxacin and carbapenems (imipenem and meropenem) were determined as 25% and 4.5%, respectively. WGS analysis indicated the presence of different resistome, but fosA genes in all isolates, and different combinations of mutations in gyrA, parC, oprD, efflux pump, and genes playing a role in the regulation of ampC gene expression. Different sequence types (STs) and serotypes were found in representative isolates with the occurrence of the O11-ST235 clone, which is a worldwide multidrug-resistant high-risk clone representing a serious public health threat. The findings of this study provide valuable information on P. aeruginosa isolated from clinical bovine mastitis cases and current antimicrobial resistance levels and virulence determinants.
DergiPark (Istanbul University), Sep 1, 2007
Bu çalışmanın amaçları solunum sistemi hastalığı semptomu bulunan hindilerden B. avium'a maruz ka... more Bu çalışmanın amaçları solunum sistemi hastalığı semptomu bulunan hindilerden B. avium'a maruz kalmış olanların serolojik olarak saptanması için lam aglutinasyon (LAT), mikroaglutinasyon (MAT) test antijenlerinin hazırlanması ve bu testlerin duyarlılıklarının ticari enzyme-linked immunosorbent assay (ELISA) ile karşılaştırılmasıdır. Çalışmanın materyalini İzmir İl'i ve çevresinde bulunan, solunum sistemi problemi gösteren 900 ticari hindiden alınan kan serum örnekleri oluşturmaktadır. Seroprevalens ELISA, LAT ve MAT için sırasıyla % 21.6, % 13.8 ve % 12.1 olarak belirlenmiştir. Sonuç olarak; B. avium'a karşı oluşan antikorların belirlenmesinde en duyarlı testin ELISA olmasına karşın; LAT ve MAT'lerinin de bu amaçla kullanılabileceği tespit edilmiştir. Anahtar sözcükler: B. avium, enzyme-linked immunosorbent assay, lam aglutinasyon testi, mikroaglutinasyon testi A comparative study on detection of Bordetella avium antibodies in turkeys by enzyme-linked immunosorbent assay, microagglutination and serum plate agglutination tests Summary: The aims of this study were to develop a serum plate agglutination test (LAT) antigen and microagglutination (MAT) test antigen for the serologic detection of turkeys that have been exposed to B. avium with respiratory disease symptom and to compare sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with these tests. All serum samples were collected from commercial turkey enterprises in İzmir, samples from 900 turkeys with symptoms of respiratory disease constituted the material of this study. The seroprevalence was determined as 21.6 %, 13.8 %, 12.1 % for ELISA, LAT, and MAT tests, respectively. As a conclusion, although ELISA was defined as the most sensitive test for screening of B. avium antibodies with this study, LAT and MAT's could also be used for this purpose.
Archives of Razi Institute, 2020
Bordetellosis or turkey coryza, caused by Bordetella avium, has been an issue for turkey industry... more Bordetellosis or turkey coryza, caused by Bordetella avium, has been an issue for turkey industry since its first description in 1967 when it was reported for the first time. Bordetella avium causes a highly contagious upper respiratory disease in turkeys. Therefore, this study aimed to isolate and characterize this species from commercial and backyard turkeys in Tehran, Isfahan, and Northern provinces of Iran. For the purpose of the study, 625 tracheal swabs were taken from 425 commercial poults and 200 backyard poults aged 2-6 weeks from September 2016 to September 2018. The swabs were immediately plated on MacConkey and blood agar plates and then pooled (5 swabs/pool) in tubes, containing 2 mL distilled water, to perform direct polymerase chain reaction (PCR) for the identification of B. avium. A total of 17 swab pools were found to be positive for B. avium. A subset of seven positive samples were sequenced for the flanking region of piuA gene. The analysis of the sequences indic...
Minerva biotechnology and biomolecular research, Mar 1, 2023
Van veterinary journal, Jul 26, 2019
Brucellosis is one of the most important zoonotic diseases transmitted to humans from animals or ... more Brucellosis is one of the most important zoonotic diseases transmitted to humans from animals or animal products. Brucella melitensis is the most pathogenic species in the genus brucella. The outer membrane protein 31 (Omp31) of B. melitensis is considered to be a protective immunogen and an important candidate vaccine. In this study, cloning from B. melitensis Rev 1 strain of Omp31 coding gene (omp31) was aimed. Brucella melitensis REV-1 live vaccine strain was used in the study. After reactivation of strain, DNA was extracted from bacterial culture. Gene sequence which encodes outer membrane protein was obtained from Gene Bank. Primers were designed to clone this region. After primer design, the gene region amplified by the polymerase chain reaction was cloned into the pET28a expression vector and transformed into Escherichia coli BL21 bacteria. Plasmid extraction was performed from E. coli BL21. The insert and plasmid were separately observed as a result of cutting with the restriction enzyme used for cloning. Expected size (790 bp) insert amplified with cloning primers again and amplicon was sequenced. The sequence obtained after sequencing analysis was compared to the gene bank and confirmed to be the outer membrane protein of B. melitensis. Further studies are required to investigate the antigenic properties of the cloned recombinant outer membrane protein Omp31 (rOmp31) and determine the potential to be a candidate for vaccination.
Kocatepe Veterinary Journal, Mar 1, 2016
Bu calismada, Turkiye’nin batisinda broyler ve yumurtaci tavuk kan serumlarinda Mycoplasma gallis... more Bu calismada, Turkiye’nin batisinda broyler ve yumurtaci tavuk kan serumlarinda Mycoplasma gallisepticum ve Mycoplasma synoviae antikorlarinin varligi ve yayginliginin ELISA ile tespit edilmesi amaclandi. Calisma materyalini, asisiz broyler ve yumurtaci tavuklardan, tesadufi ornekleme yolu ile 60 kumesten alinan, toplam 1060 kan ornegi olusturdu. Uc ilden alinan 700 broylerde M. gallisepticum ve M. synoviae seropozitiflik oranlarinin illere gore gruplar arasi istatistiksel karsilastirilmasi icin Ki-Kare testi kullanildi. Her uc ildeki 700 broylerin %26,4’u M. gallisepticum, %60,2’si M. synoviae antikorlari yonunden pozitif olarak tespit edilirken; illere gore seropozitiflik oranlari arasi fark istatistiksel olarak onemli (P<0,001) bulundu. Bir ilden alinan 360 yumurtaci tavuk kan serumunun %65,0’i M. gallisepticum, %82,7’si M. synoviae antikorlari yonunden pozitif idi. Alinan 1060 serum ornegi toplu olarak degerlendirildiginde ise %39,5 M. gallisepticum, %67,9 M. synoviae seropozitif olarak belirlendi. Bu sonuclar, incelenen isletmelerde ozellikle M. synoviae infeksiyonlarinin yaygin olarak goruldugunu ortaya koydu. Isletmelerde mikoplazma negatifliginin belirlenmesi amaci ile tarama testlerinin belirli araliklarla yapilmasi ve “Mikoplazma Kontrol Programlari”nin olusturulmasi gerektigi sonucuna varildi.
Turkish Journal of Veterinary & Animal Sciences, Feb 25, 2008
The aims of this study were to isolate Pseudomonas aeruginosa from dogs with otitis externa (OE),... more The aims of this study were to isolate Pseudomonas aeruginosa from dogs with otitis externa (OE), to determine the susceptibility of isolated strains to antibiotics, and to evaluate the incidence of the infection in the Ayd›n region. For this purpose, ear swab samples were obtained from 92 dogs with symptoms of OE infection and the samples were examined bacteriologically. Bacteria were isolated from 88 (96%) of 92 samples but no isolation was performed on 4 (4%) dogs. In total 93 microorganisms were identified. Among these microorganisms, staphylococci species were the most isolated one in number (43%), followed by P. aeruginosa (17%), Escherichia coli (11%), Proteus spp. (9%), Streptococcus spp. (9%), Pasteurella spp. (3%), Citrobacter spp. (3%), Corynebacterium spp. (3%), and Enterococcus spp. (2%). Of the 16 P. aeruginosa strains examined, 13 (81%) were sensitive to gentamycin, 12 (75%) to penicillin G, 7 (44%) to danofloxacin, 6 (38%) to streptomycin, 3 (19%) to ampicillin, 2 (13%) to lincomycin, and 2 (13%) to tetracycline. In conclusion, gentamycin and penicillin could be more effective for the treatment of OE caused by P. aeruginosa in the Ayd›n region.
Zoonoses and Public Health, May 1, 2010
The aim of this study was to identify methicillin-resistant Staphylococcus aureus (MRSA) strains ... more The aim of this study was to identify methicillin-resistant Staphylococcus aureus (MRSA) strains gathered from 2002 to 2006 from milk samples in Aydin region in Turkey. Among 93 S. aureus strains isolated from bovine milk with mastitis, 16 were resistant to methicillin. Methicillin-resistant S. aureus strains were studied further for their staphylococcal cassette chromosome mec (SCCmec) types, pulsotypes, spa and MLST types, antimicrobial susceptibilities, mechanisms of resistance and presence of Panton-Valentine leucocidin (PVL) toxin gene. The MRSA strains were multi-drug resistant. The susceptibility rates to antimicrobials tested were 0%, 0%, 0%, 0%, 6.25%, 16.25% and 56.25% for erythromycin, clindamycin, chloramphenicol, gentamicin, tetracyclin, ciprofloxacin and vancomycin, respectively. All tetracycline and gentamicin resistant strains carried tet(M) and aac(6)-aph(2) gene, respectively. Among macrolide-resistant isolates, nine had erm(A), and seven had both erm(A) and erm(B) genes. The molecular characterization by pulsed-field gel electrophoresis showed presence of three pulsotypes with their variants. The pulsotype B strains were type IV with SCCmec typing, and representative of pulsotype B was t190 by spa typing and ST8 by MLST typing. The strains with pulsotype A and C were SCCmec III, and representative of these pulsotypes was t030 by spa typing. The MLST type of pulsotype A was ST239 and pulsotype C was one allele variant of ST239. None of the isolates harboured the PVL gene. Presence of hospital-related MRSA strains may indicate transmission of these strains between human and animals. In case of clonal spread beside the infected animals&#39; treatment of MRSA carrier, farm workers should also be considered. Hygienic measures and rational antibiotic use may avoid resistance selection, clonal dissemination of resistant strains and decrease losses because of mastitis in dairy herds.
DergiPark (Istanbul University), Jul 26, 2019
Brucellosis is one of the most important zoonotic diseases transmitted to humans from animals or ... more Brucellosis is one of the most important zoonotic diseases transmitted to humans from animals or animal products. Brucella melitensis is the most pathogenic species in the genus brucella. The outer membrane protein 31 (Omp31) of B. melitensis is considered to be a protective immunogen and an important candidate vaccine. In this study, cloning from B. melitensis Rev 1 strain of Omp31 coding gene (omp31) was aimed. Brucella melitensis REV-1 live vaccine strain was used in the study. After reactivation of strain, DNA was extracted from bacterial culture. Gene sequence which encodes outer membrane protein was obtained from Gene Bank. Primers were designed to clone this region. After primer design, the gene region amplified by the polymerase chain reaction was cloned into the pET28a expression vector and transformed into Escherichia coli BL21 bacteria. Plasmid extraction was performed from E. coli BL21. The insert and plasmid were separately observed as a result of cutting with the restriction enzyme used for cloning. Expected size (790 bp) insert amplified with cloning primers again and amplicon was sequenced. The sequence obtained after sequencing analysis was compared to the gene bank and confirmed to be the outer membrane protein of B. melitensis. Further studies are required to investigate the antigenic properties of the cloned recombinant outer membrane protein Omp31 (rOmp31) and determine the potential to be a candidate for vaccination.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2002
... 118 : 151-161. 1991. Kwok S, Higuchi R: Avoiding false positives with PCR. Nature. ... Vet J,... more ... 118 : 151-161. 1991. Kwok S, Higuchi R: Avoiding false positives with PCR. Nature. ... Vet J, 153: 2~87-305. 1997. 17 Saiki KR, Gelfand HD, Steffi S, Scharf .IS, Higuchi R, Horn TG: Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. ...
Israel Journal of Veterinary Medicine, 2009
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2013
The aim of the study was to investigate the occurence and species distribution of methicillin res... more The aim of the study was to investigate the occurence and species distribution of methicillin resistant staphylococci (MRS) in the nasal cavity of dogs. Nasal swabs were collected from 162 dogs entering private veterinary clinics in Hatay. Methicillin resistance was detected onto mannitol salt agar containing 2 µg/ml oxacillin and confirmed by mecA Polymerase Chain Reaction (PCR). Bacterial identification was done using 16S rRNA sequencing. Staphylococcal cassette chromosome mec (SCCmec) typing of these isolates were determined by multiplex PCR. Antimicrobial susceptibility testing were performed disk diffusion method and antimicrobial resistance genes were determined by PCR. Methicillin-resistant coagulase negative staphylococci (MRCNS) harbouring mecA were isolated from 15.4% (25/162) of dogs. The species identified were S. epidermidis (n=12), S. lentus (n=6), S. hominis (n=4), S. warneri (n=1), S. arlettae (n=1) and S. haemolyticus (n=1). mecA-mediated methicillin resistance in S. arlettae was described for the first time. Methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant Staphylococcus pseudintermedius (MRSP) were not detected. SCCmec type I, II, III and IV were identified in 1, 10, 9 and 5 MRS isolates, respectively. The results indicate that continuous surveillance is necessary to determine the emergence of MRS including MRSA.
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Papers by SUHEYLA TURKYiLMAZ