Papers by Siti Sunarintyas
Advanced structured materials, 2016
Biomaterials are widely used in many kinds of medical devices. The biomaterials used can be metal... more Biomaterials are widely used in many kinds of medical devices. The biomaterials used can be metal, polymer, ceramic or composites. Bioadhesion will be occured when the medical device contact to biological surface. There are many conditions where bioadhesion is beneficial and vice versa. The implantation of medical devices in the human body is not without risk. It is reported that implantable medical devices are an ideal interface for microorganisms. There are infections caused mainly by bacteria originating in the body. Some aspects influence bioadhesion of implantable medical devices including surface topography, chemical interaction, mechanical interaction and physiological interactions are discussed. The understanding of such aspects hopefully governs medical practitioners in controlling the medical devices bioadhesion process, then optimizing the desirable bioadhesion and removing the undesirable interactions. To complete the discussion on bioadhesion of biomaterials, it is also described some methods of bioadhesion testing including surface roughness measurement, contact angle measurement, surface topography evaluation and biofilm formation testing. At last, a perspective related to biomaterials used as medical devices is presented.
Inorganic Chemistry Communications, Mar 1, 2023
Poly(L-lactic acid) or PLLA as one of tissue engineering material have demonstrated low cell inte... more Poly(L-lactic acid) or PLLA as one of tissue engineering material have demonstrated low cell interaction capability due to its hydrophobicity. Bombyx mori's sericin, a polymer protein that has strongly polar side groups such as hydroxyl, carboxyl and amino groups, have been known to accelerate cells attachment and proliferation. The aim of this study was to investigate the effect of Bombyx mori's sericin immobilization with different concentration over PLLA surface on attachment and proliferation of mesenchymal stem cells (MSCs). Three concentration of sericin (2.5, 5, and 10 mg/ml) were applied on PLLA surface modification using carbodiimide chemistry. Surface characterization using Fourier Transform-Infrared Spectroscopy (FTIR) and water contact angle was performed to observe the changes on surface-modified PLLA. The MSCs attachment after 4 hours in culture and proliferation rate in 2 days were determined using hemocytometer. FTIR graphs showed that sericin was succesfully immobilized on PLLA surface. Contact angle measurement showed significant increases of the hydrophilicity on sericin-modified PLLA surfaces. MSCs attached to sericin-modified PLLA was higher in number than the cell attached on control PLLA (p<0.05), but there was no difference between the treatment groups(p>0.05). However, the proliferation rate of sericin-modified PLLA has no significant differences with control PLLA (p>0.05). The conclusion was Bombyx mori's sericin application over PLLA surface enhanced MSCs attachment, but did not enhanced their proliferation rate.
Dental Journal: Majalah Kedokteran Gigi, Jun 15, 2020
Background: Composite resins are widely used in dentistry to restore dental caries. Recently, sho... more Background: Composite resins are widely used in dentistry to restore dental caries. Recently, short fibre-reinforced composite (FRC) resins have been widely used for high-stress areas, especially in posterior teeth. Bombyx mori silk fibre is under research to reinforce dental composite resin as it has good mechanical properties. Purpose: This study aims to obtain the effects of alkalisation and silk fibre volume fraction on the flexural strength of FRC. Methods: Bombyx mori silk fibres were obtained from Perhutani, Pati, Indonesia. Samples were divided into two alkalisation groups (4% and 8%). Alkalisation of the silk fibres was conducted through the scouring process in NaOH, hydrolysis (30% H 2 SO 4) and drying. Silk fibres were then reinforced in a resin matrix. The samples were subdivided based on the fibre volume fraction reinforcements, which were 0%, 5%, 10% and 15%. Each group of samples consisted of three specimens (n = 3). Flexural strength was measured using a universal testing machine. Data were analysed by two-way ANOVA (p < 0.05) and post-hoc least significant difference test (p < 0.05). Results: The results showed the flexural strength (MPa) means of the 4% alkalisation group were 169.
Contemporary Clinical Dentistry, 2020
Background: The success of root canal treatment is influenced by hermetic root canal obturation. ... more Background: The success of root canal treatment is influenced by hermetic root canal obturation. This study was conducted to analyze the apical sealing ability after the addition of calcite-synthesized hydroxyapatite (HA) as an epoxy resin sealer filler. Methods: Calcite-synthesized HA powder was prepared using the microwave hydrothermal process. HA resin sealer powder and epoxy resin paste (3:1) were mixed, and concentrations of 10%, 20%, 30%, 40%, and 50% were prepared. A sample of thirty maxillary incisors were prepared in the root canal and then, the crown was cut to leave 13 mm of the root and a working length of 12 mm. The root canal was prepared using the crown-down technique and irrigated using 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid alternately. The samples were divided into six groups, with each group consisting of five roots. Group I was obturated with gutta percha using an epoxy resin sealer without HA (HA-0%) as a control group. In each of the Groups II, III, IV, V, and VI, 10% HA resin sealer, 20% HA, 30% HA, 40% HA, and 50% HA were used. All the samples were incubated in a 10-ml simulated body fluid solution at 37°C for 4 weeks. Apical closure density measurement was done using a scanning electron microscope, and the results were analyzed using the Kruskal–Wallis and Mann–Whitney U-tests. Results: A significant increase in the apical sealing ability was observed in the HA-20% sealer group and the HA-30% and HA-40% groups compared to that in the control group. However, the HA-50% sealer group showed a decrease in the apical sealing ability, whereas the HA-10% sealer group showed no difference. The HA-30% had the highest sealing ability than other concentrations. Conclusion: The addition of calcite-synthesized HA as a filler at concentrations of 20%, 30%, and 40% increased the apical sealing ability of the epoxy resin sealer.
Majalah kedokteran gigi Indonesia, Dec 19, 2018
ABSTRAK Ketersediaan E-glass fiber dental untuk restorasi fiber reinforced composite (FRC) di Ind... more ABSTRAK Ketersediaan E-glass fiber dental untuk restorasi fiber reinforced composite (FRC) di Indonesia terbatas dengan harga relatif mahal, sehingga glass fiber non dental digunakan sebagai alternatif. Glass fiber non dental memiliki komposisi yang hampir sama dengan E-glass fiber dental. Salah satu parameter penting untuk kekuatan FRC adalah impregnasi fiber dengan resin. Fiber sulit berimpregnasi dengan resin yang memiliki viskositas tinggi. Penelitian ini bertujuan untuk mengetahui pengaruh impregnasi glass fiber non dental menggunakan resin bis-GMA dan TEGDMA terhadap kekuatan fleksural FRC. Bahan yang digunakan dalam penelitian adalah glass fiber non dental (CMAX, China), resin bis-GMA (Sigma-Aldrich, USA), dan TEGDMA (Sigma-Aldrich, USA). Sampel 3 kelompok perlakuan terdiri dari FRC dengan fiber tanpa impregnasi, FRC dengan fiber terimpregnasi (bis-GMA:TEGDMA=4:1) dan FRC dengan fiber terimpregnasi (bis-GMA:TEGDMA=1:1) (n=12). Ketiga kelompok diuji kekuatan fleksural berdasarkan ISO 10477. Sampel direndam dalam akuades dan disimpan pada suhu 37 °C selama 24 jam sebelum uji kekuatan fleksural. Hasil yang diperoleh dianalisis variansi satu jalur dan post hoc LSD. Hasil penelitian menunjukkan rerata kekuatan fleksural terendah (116,16 ± 15,87MPa) pada kelompok FRC dengan fiber tanpa impregnasi dan tertinggi (151,32 ± 23,74 MPa) pada kelompok FRC dengan fiber terimpregnasi (bisGMA:TEGDMA=1:1). Analisis statistik menunjukkan impregnasi fiber memberikan pengaruh signifikan (p< 0,05). Kesimpulan penelitian adalah impregnasi glass fiber non dental menggunakan resin bis-GMA dan TEGDMA dapat meningkatkan kekuatan fleksural FRC dan FRC dengan glass fiber non dental terimpregnasi (bis-GMA:TEGDMA=1:1) memiliki kekuatan fleksural paling tinggi. Kata kunci: glass fiber non dental; impregnasi fiber; kekuatan fleksural ABSTRACT: Non-Dental glass fiber impregnation on flexural strength of fiber reinforced composite. The availability of dental E-glass fibers for Fiber Reinforcement (FRC) restoration is limited in Indonesia with relatively high cost.Therefore, non-dental glass fibers have been used as an alternative material. The composition of non-dental glass fibers is almost the same with dental E-glass fibers. An important parameter responsible for the strength of FRC is the impregnation of the fibers with resin. Reinforcing fibers are difficult to impregnate with the resin systems of high viscosity. The aim of study is to assess the effect of non-dental glass fibers impregnation using bis-GMA and TEGDMA resin on the flexural strength of FRC. The materials used in the study were non-dental glass fibers (CMAX, China), resin bis-GMA (Sigma-Aldrich, USA) and TEGDMA (Sigma-Aldrich, USA). Three groups of samples consisted of FRC with non-impregnated fibers, FRC with impregnated fibers (bis-GMA:TEGDMA=4:1) and FRC with impregnated fibers (bis-GMA:TEGDMA=1:1). The three groups were tested to determine flexural strength according to ISO 10477. The samples were stored in aquadest at 37 °C for 24 hours prior to flexural strength test. The results were analyzed by one way ANOVA with LSD post hoc test. The results of study showed that the lowest mean of flexural strength (116.16 ± 15.87MPa) was FRC with non-impregnated fiber and the highest mean (151.32 ± 23.74 MPa) was FRC with impregnated fiber (bis-GMA:TEGDMA=1:1). The statistical analysis showed that fiber impregnation had a significant effect (p< 0.05). It can thenbe concluded that non-dental glass fiber impregnation using bis-GMA and TEGDMA resin could increase the flexural strength of FRC and FRC with the impregnated fibers (bis-GMA:TEGDMA=1:1) that had the highest mean of flexural strength.
Dental Journal: Majalah Kedokteran Gigi, Dec 1, 2013
Background: Direct contact between the bleaching agent and the enamel surface results in deminera... more Background: Direct contact between the bleaching agent and the enamel surface results in demineralization, alteration in surface roughness and bacterial adhesion. Many studies try to minimize this side effect through different way. Purpose: The aim of this study was to determined the effect of Calcium Phospho Peptide-Amorphous Calcium Phosphate (CPP-ACP) containing fluoride application before and after bleaching procedure on the adhesion of S. Mutans and enamel roughness. Methods: The samples were 6 teeth which were divided into 4 groups, and each tooth was cut into four pieces. Group A and C were treated with CPP-ACP after bleaching, while group B and D were treated with CPP-ACP before and after bleaching. CPP-ACP used in group C and D was the one that contain fluoride. After treatment, all samples were sterilized, immersed in steril human saliva for one hour, then immersed into S. mutans suspension of 108 CFU. Samples were incubated overnight. On the next day the samples were put into steril BHI and vortexed for one minute to detach the bacteria. Fifteen ml BHI containing bacteria was poured into TYS agar then incubated 37°Cfor 48 hours. Bacterial colony was counted with colony counter. The SEM examination was done on all samples. Results: Application of desensitizing agent reduced the S.mutans adhesion significantly among groups (p<0.05) except between group A and C. SEM evaluation revealed significant differences among groups. Conclusion: The application of CPP-ACP containing fluoride before and after bleaching was effective to reduce the accumulation of S.mutans colony and enamel roughness.
Dental Materials, 2013
Purpose: Fiber-reinforced composite (FRC) is consists of matrix that is reinforced with thin fibe... more Purpose: Fiber-reinforced composite (FRC) is consists of matrix that is reinforced with thin fibers, which have high tensile strength and flexural modulus (Zhang and Matinlinna. Silicon 2012 4: 73). The bis-GMA-MMA combination as the resin matrix is widely used as resin matrix, whereas bis-GMA is reported as the most cytotoxic monomer among dental resin composite monomers (Moharamzadeh et al. Material 2009 2:514). Resin matrix 1,6 hexanediol dimethacrylate (HDMA) has similar reactive groups than bis-GMA, and is not listed as carcinogens (Vallittu and Sevelius. J Prosthet Dent 2000 84: 413). The purpose of this study was to investigate the water sorption of a new resin matrix system of FRC based on HDMA. Materials and Methods : Materials used were: E-glass fibre bundles (Stick Tech, Finland), HDMA (Esstech, USA), bis-GMA (Sigma-Aldrich, USA), MMA (ProSciTech, Australia), CQ (Esstech, USA) and CEMA (Esstech, USA).: Fifteen specimens (2mm x 2mm x 25mm) were prepared and divided into 3 groups. Composition of group-1: 78.4%HDMA+19.6%MMA+1.0%CQ+1.0%CEMA;group-2:49.0%HDMA +49.0%MMA + 1.0%CQ +1.0%CEMA; and group-3 :78.4% bis-GMA +19.6%MMA +1.0%CQ+ 1.0%CEMA. Specimens with two fiber rovings of 25 mm long were placed in a mould, and monomers were added and light-cured with halogen light-curing unit on both sides for 3 x 40s. Specimens were immersed in 15 ml distilled water of 37 o C for 21 days. The difference in weights, before and after immersion were recorded. Data obtained were analyzed by one-way ANOVA and LSD. Results: As for the difference in weights (before and after immersion), group-1 showed the lowest average (0.004%0, followed by group-3 (0.003%) and group-2 (0.01%). Statistical analysis (ANOVA) proved a significant difference among the three groups (p<0.05). Result of LSD showed there was a significant difference between group-1 and group-2 (p<0.05) but no significant difference between group-1 and group-3 (p>0.05). Conclusions: It can be concluded that FRC based on HDMA matrix system (group-1) is comparable to bis-GMA (group-3) on its water sorption.
Nucleation and Atmospheric Aerosols, 2020
One potential and useful product of Indonesia's biodiversity is fibroin which extracted from silk... more One potential and useful product of Indonesia's biodiversity is fibroin which extracted from silkworm cocoons of Bombyx mori L (silk fibroin). Silk fibroin, in which has capability to fuse into the human body, is used for bone graft or wound healing dressing. In the dentistry, researchers develop silk fibroin as a dental treatment material for pulp perforation teeth case. The dental treatment material developed from silk fibroin is expected to overcome the limitation of existing materials. The purpose of this study was to determine the molecular weight and structural characteristics identification of silk fibroin as a preliminary study before being used as dental material. This research used two methods, which were sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS PAGE) and Fourier transform infrared Spectroscopy (FTIR). The result showed that there was an expression of electrophoresis bands on the molecular weight of 10-55 kDa, while the FTIR showed the peak spectra of fibroin sample was at 3309.80 cm-1. Based on its molecular weight and structure characteristic, fibroin consists of an NH group of protein that consists of several amino acids.
Dental Journal: Majalah Kedokteran Gigi, Dec 19, 2015
Background: Fiber reinforced composite (FRC) is can be used in dentistry as an orthodontic retain... more Background: Fiber reinforced composite (FRC) is can be used in dentistry as an orthodontic retainer. FRC still has a limitations because of to a weak bonding between fibers and matrix. Purpose: This research was aimed to evaluate the effect of silane as coupling agent and fiber impregnation on the tensile strength of E-glass FRC. Methods: The samples of this research were classified into two groups each of which consisted of three subgroups, namely the impregnated fiber group (original, 1x addition of silane, 2x addition of silane) and the non-impregnated fiber group (original, 1x addition of silane, 2x addition of silane). The tensile strength was measured by a universal testing machine. The averages of the tensile strength in all groups then were compared by using Kruskal Wallis and Mann Whitney post hoc tests. results: The averages of the tensile strength (MPa) in the impregnated fiber group can be known as follow; original impregnated fiber (26.60±0.51), 1x addition of silane (43.38±4.42), and 2x addition of silane (36.22±7.23). The averages of tensile strength (MPa) in the non-impregnated fiber group can also be known as follow; original non-impregnated fiber (29.38±1.08), 1x addition of silane (29.38±1.08), 2x addition of silane (12.48±2.37). Kruskal Wallis test showed that there was a significant difference between the impregnated fiber group and the non-impregnated fiber group (p<0.05). Based on the results of post hoc test, it is also known that the addition of silane in the impregnated fiber group had a significant effect on the increasing of the tensile strength of E-glass FRC (p<0.05), while the addition of silane in the non-impregnated fiber group had a significant effect on the decreasing of the tensile strength of E-glass FRC. Conclusion: It can be concluded that the addition of silane in the non-silanated fiber group can increase the tensile strength of E-glass FRC, but the addition of silane in the silanated fiber group can decrease the tensile strength of E-glass FRC. It is also known that the impregnation of fiber can increase the tensile strength of E-glass FRC.
International Journal of Biomaterials, 2012
Synthetic hydroxyapatite (HA) has been widely used and developed as the material for bone substit... more Synthetic hydroxyapatite (HA) has been widely used and developed as the material for bone substitute in medical applications. The addition of zirconia is needed to improve the strength of hydroxyapatite as the bone substitute. One of the drawbacks in the use of biomedical materials is the occurrence of biomaterial-centred infections. The recent method of limiting the presence of microorganism on biomaterials is by providing biomaterial-bound metal-containing compositions. In this case, S. epidermidis is the most common infectious organism in biomedical-centred infection. Objective. This study was designed to evaluate the effect of zirconia concentrations in hydroxyapatite on the growth of S. epidermidis. Methods and Materials. The subjects of this study were twenty hydroxyapatite discs, divided into four groups in which one was the control and the other three were the treatment groups. Zirconia powder with the concentrations of 20%, 30%, and 40% was added into the three different treatment groups. Scanning electron microscope analysis was performed according to the hydroxyapatite and hydroxyapatite-zirconia specimens. All discs were immersed into S. epidermidis culture for 24 hours and later on they were soaked into a medium of PBS. The cultured medium was spread on mannitol salt agar. After incubation for 24 hours at 37 • C , the number of colonies was measured with colony counter. Data obtained were analyzed using the ANOVA followed by the pairwise comparison. Result. The statistical analysis showed that different concentrations of zirconia powder significantly influenced the number of S. epidermidis colony (P < 0.05). Conclusion. The addition of zirconia into hydroxyapatite affected the growth of S. epidermidis. Hydroxyapatite with 20% zirconia proved to be an effective concentration to inhibit the growth of S. epidermidis colony.
Key Engineering Materials, Apr 1, 2020
One of the requirements of endodontic material is to have good biocompatibility of pulp tissue th... more One of the requirements of endodontic material is to have good biocompatibility of pulp tissue that has direct contact with the material, to promote the process of tissue repair. Reversible pulpitis needs direct pulp capping treatment with medicament materials i.e. RMGIC (Resin-Modified Glass Ionomer Cement) and MTA (Mineral Trioxide Aggregate) which are used as the gold standard for direct pulp capping treatment. Both of the materials have several disadvantages including the necrotic tissue in the area has direct contact with the pulp so that it affects the successful treatment results. Nowadays, the new materials are being developed which are expected to improve the existing material deficiencies. In this study, fibroin was extracted from Bombyx mori L. cocoon. This study aimed to examine the biocompatibility of fibroin as a new pulp capping material with RMGIC (Fuji II LC, GC, Japan) and MTA (Rootdent, Technodent, Russia). An experimental study was conducted using extracted human primary dental pulp cells in vitro through orthodontic treatment. A methyl thiazole tetrazolium (MTT) assay was employed to test biocompatibility using ELISA Reader 590 nm wavelengths for 24, 48, and 72 h, respectively. The findings showed that the biocompatibility of fibroin had the highest value of all. In conclusion, fibroin biocompatibility toward MTA and RMGIC as pulp capping materials can be aligned, however, there were no significant differences.
Metal is common as medical devices. Stainless steel (SS316L) has low carbon composition in order ... more Metal is common as medical devices. Stainless steel (SS316L) has low carbon composition in order to increase corrosion resistance. The release of stainless steel ions can occur due to corrosion and affect the surface roughness. This study determined the influence of pH level of saliva on the concentration of nickel ions release and the surface roughness of SS316L. Twelve specimens of SS316L sized (10x10x1)mm were divided into 3 groups (artificial saliva with pH 3, 5, and 7). Samples were stored in the incubator at 37°C for 14 days. The concentration of nickel ions released was measured using atomic absorption spectrophotometry (ppm). The surface roughness was measured before and after the immersion using surface roughness measuring instrument (µm). The result showed the concentration of nickel ion released (ppm) were 0.099±0.023 (pH 3), 0.045±0.020 (pH 5), and 0.000±0.000 (pH 7). While the surface roughness (µm) were 0.22±0.06 (pH 3), 0.12±0.04 (pH 5), and 0.1±0.03 (pH 7). One-way ANOVA proved that the pH level of saliva significantly influenced the concentration of nickel ions and the surface roughness (p <0.05). It can be concluded that the lower pH level of saliva, the higher concentration of nickel ions released and the rougher the surface of SS316L.
An ideal stent is stent with good endurance against pressure from its surrounding so that the ste... more An ideal stent is stent with good endurance against pressure from its surrounding so that the stent failure can be avoided. Geometry analysis can be used to find out the stent characteristics before and after expanded using balloon angioplasty. The aim of this research is to evaluate the free expansion behavior of coronary stent based on the geometry of strut linker using finite element method. Pressure load was added into inner surface of stent to get the von Mises stress maximum value and its distribution. The results showed that strut linker geometry affected free expansion behavior.
This study determined the antibacterial effect of ethanolic neem leaves and stick extract in inhi... more This study determined the antibacterial effect of ethanolic neem leaves and stick extract in inhibiting the growth of Sreptococcus mutans. Materials and Methods: Two different parts of neem, leaves and stick, extracts using ethanol were prepared at 10% and 20% (w/v: extraction powder/water) respectively. Each extractions were dropped on an MHA agar that had been inoculated with Streptococcus mutans. Distilled water was used as the control. After 24 hours of incubation, the inhibition diameters were measured. Collected data were analyzed by analysis of variance (ANOVA) followed by Least Significance Difference (LSD) at a 95% confinement level. Results: The inhibition zone value of neem extracts on Streptococcus mutans showed that neem leaves extract had less inhibition value than neem stick extract on all concentrations. The ANOVA showed that there were significant influence of neem extracts (p<0.001), neem concentrations (p<0.001), and neem extractconcentration (p<0.023) on Streptococcus mutans inhibition. Conclusion: Neem leaves and stick ethanolic extracts had antibacterial effect on Streptococcus mutans. Neem stick extract had higher antibacterial properties than the leaves extract.
Human devices are needed to overcome medical problems. The success of human devices is much relat... more Human devices are needed to overcome medical problems. The success of human devices is much related to the satisfaction of the patient. Factors affecting patient’s satisfaction could be viewed from the doctor’s aspect or from the device itself. From the device’s point of view, considerations should be taken on the properties of the biomaterial, such as the surface characterization. Surface properties of a biomaterial will affect the adherence of biofilm that may lead to a failure of the human devices (such as infection). There is a big challenge for the medical experts, biomaterial scientists and biomedical engineers in preventing such infections caused by human devices. Key words: biomaterial, human devices
Journal of Dentistry Indonesia, 2003
Biomareriai. Fakultas KcdoLleri]]l Ciigi l, ni\ crsilas Cadjah Mada Siti SLrnarinlvas. LaDra I'em... more Biomareriai. Fakultas KcdoLleri]]l Ciigi l, ni\ crsilas Cadjah Mada Siti SLrnarinlvas. LaDra I'embcrsihar IUsien papaLr) pada I'elepa\an Plak Gigi l rruan. .lurnal Kedokreran Gier Uni\efsitas lndone,iia. l00li lrliEdLsl KhusLrs) :l l ll5 E!erlda) denru'e cleansing l\ ncc.led lo pI.\enl lhe nrouth tion dcnlurc sionatitis and esthelic concern. Erz!rne cleansers arc d.vclopcC in anerrrpt io break dost Ihe organic componenls of dennne plaque The ailn ofthis res.arch is to deremine lhe efticienl clearsing drarion ofpaparn solution to renx'vc:J-hour denture plaque. ihe fesearch $r' don. on l6 patienls wlth complerc dcntures Plaque rcmolal was detennired b) Lo$r) mclhod and SDS t \Gt The resLrll sho\s that papain dose reqrrired to htdrol\ze 14-hour derlin. plaquc is l5 66 Tti'r1-j crrz)rre acli!it) fot l0 minutes soaking. Soaking lhe denrLrre in papain iolution ibr l0 minutcs lnakes rll ofrhe detected pldquc proLein band! renrole. In conclusion. efilcienl cleansing durarion ol papan 'oiLrrion to remo\e 2,1-hour dcnture plaque rs prprin acllvLlt of 15.66 IL'mg by rcaknrg du.alion ol lfi nrinLrles. Fu(her research i! suggestcd lo c\arnine paparrr rc\ldLrc on the denlure that mlv inllucncc lhc dcnlure wearef bioconrpatibilit),.
Dental Journal: Majalah Kedokteran Gigi, Dec 30, 2017
Background: Restoration of the teeth immediately after bleaching with 35% hydrogen peroxide (H 2 ... more Background: Restoration of the teeth immediately after bleaching with 35% hydrogen peroxide (H 2 O 2) is contraindicated due to the remnant of free radicals that will stay inside enamel and dentin for 1-3 weeks and reduce the adhesion of composite resin. Sodium ascorbate is an antioxidant substance known to bind free radical residues, thereby shortening the delay in restoration. Purpose: The purpose of this study was to examine the resin bond strength of bleached dentin influenced by the application of 35% sodium ascorbate. Methods: Nine premolars were divided into their crown and root sections, with the crown subsequently being cut into four equal parts to obtain 36 samples. These were then divided into four groups, each containing nine samples. Group A (control): samples were bleached using35% hydrogen peroxide, immersed in an artificial saliva, stored in an incubator at 37°C for seven days and then filled with a composite resin. Group B:samples were also bleached by means of 35% H 2 O 2 followed by one application of 0.025 ml 35% sodium ascorbate for 5 minutes and restored with composite resin. Group C: samples were bleached with 35% H 2 O 2 , followed by two applications of 0.025 ml 35% sodium ascorbate for 5 minutes, and restored with a composite resin. Group D: dentin was bleached with 35% H 2 O 2 followed by three applications of 0.025 ml sodium ascorbate 35% for 5 minutes and restored with a composite resin. The shear bond strength of the composite resin was measured by a universal testing instrument (Zwick, USA). Data was analyzed by means of one-way Anova and LSD. Results: The highest mean shear bond strength of composite resin was in group C, while the lowest was in group B. The result of one-way Anova indicated a difference in the shear bond strength of composite resin in the four treatment groups (p < 0.05). An LSD test showed there to be a difference in shear bond strength of composite resin between group A and groups C and D or between group B and groups C and D. There was no difference in shear bond strength of composite resin between group A and group B or between group C and group D. Conclusion: Application frequency of 35% sodium ascorbate affect on shear bond strength of composite resin restoration in bleached dentin by 35% H 2 O 2 .
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Papers by Siti Sunarintyas