International journal of drug delivery technology, Dec 24, 2023
Ayurveda is the world's oldest continuous medicinal tradition. Ayurveda's discovery of the long-l... more Ayurveda is the world's oldest continuous medicinal tradition. Ayurveda's discovery of the long-lasting single-dose forms of Asava and Arishta is a boon to modern medicine. Diarrhea, colitis, chronic cough, asthma, tuberculosis, and improved digestive function are only a few of the conditions for which the ayurvedic formulation Babbularishta is utilized. Gallic acid is one of the active ingredients in babbularishta, a commercially available ayurvedic medicine. The purpose of this study was to develop a standard for the quality of commercially available Babbularishta by measuring its alcohol content and pH. To meet World Health Organisation (WHO) standards, an high-performance liquid chromatography (HPLC) and ultraviolet (UV) method was devised for quantifying the main component, gallic acid, in babbularishta. Gas chromatography and redox titrimetry were used to confirm the accuracy of the formulation determined during the development process. The formulation was further tested for pharmacological activity, elemental makeup, pesticide traces, and heavy metal concentration.
Research Journal of Pharmacy and Technology, Dec 28, 2010
Two simple spectrophotometric: methods for the determination of ac:ec:lofenac: and paracetamol in... more Two simple spectrophotometric: methods for the determination of ac:ec:lofenac: and paracetamol in tablets have been developed. First method is based on the additivity or absorbances. Second method is based on the determination or graphical absorbance ratio at two selected wavelengths; one being the isoabsorptive point for the two drugs (230 nm) and the other being the absorption maximum of parac:etamol (256 nm). Beer-Lambert's law is obeyed for both the drugs in the concentration range 1-10 J.Lg/mL. Both the methods were found to be simple, rapid, and accurate and can be adopted in routine analysis of drugs in formulations.
Jpc – Journal Of Planar Chromatography – Modern Tlc, Aug 1, 2006
... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78.... more ... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78. [13] MS Raju, DG Sankar, and CS Sastry, Asian J. Chem. ... Sci. 86 (1997) 1030. [24] S. Lakshmi, V. Anilkumar, M. Venkatesan, and TK Raja, Indian Drugs 40(10) (2003) 589591. ...
International Journal of Health Sciences (IJHS), Jun 11, 2022
A simple, rapid, specific, precise and accurate Reverse phase high performance liquid chromatogra... more A simple, rapid, specific, precise and accurate Reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for quantitative analysis of Ketorolac tromethamine in bulk and marketed formulations such as tablet, ophthalmic solution and gel and validated as per ICH guidelines. Chromatographic separation was performed on a C18 column using an optimized mobile phase of Methanol: Water (75:25) at a flow rate of 1ml/min using the HPLC method. The detection was done at 319 nm and Ketorolac tromethamine had a retention time 2.522 minutes. The total run time of analysis was 10minutes. The drug obeyed Beers law in the concentration range of 4-12µg/ml and the calibration plot revealed a linear relationship. The LOD and LOQ for ketorolac tromethamine were determined to be 0.415 µg/ml and 1.3 µg/ml, respectively. The accuracy and repeatability studies of the proposed method was found to be in acceptable limits, %RSD was less than 2. The proposed method showed excellent linearity, specificity, accuracy, precision, LOD, LOQ and system suitability results within the acceptance criteria. This method with features of less run time and lower retention time can be used for routine analysis of Ketorolac tromethamine in bulk and pharmaceutical dosage forms and does not require any complicated methods.
Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality cont... more Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality control aspect of Ayurvedic polyherbal medicines. The modern methods can be used for standardization of these formulations. The qualitative and quantitative study of herbal drug can be accomplished by simple, rapid and cost effective UV Absorption Specrophotometric method. In the present study the Ayurvedic polyherbal formulation used as a brain tonic, was analyzed by a UV specrtophotometric method using shankhpushpin (Evolvoulous alsinoide) and asiaticoside (Centella asiatica) as marker compounds. The validation parameter as per ICH guidelines such as linearity, Precision, accuracy, limit of detection and limit of quantitation was studied. The linearity was observed in the range of 5-30μg/ml for both the markers. Limit of detection and quantitation was observed in nanograms showing high sensitivity of the developed method. The percent recovered amount of was 97.28 % Shankhpushpin and 95.69% Asiaticoside. The method was robustness and ruggedness for analysis of polyherbal formulation. The polyherbal Ayurvedic formulation can be analyzed successfully by UV Absorption Spectrophotometric method
Novel isoxazoline derivatives were synthesized by condensation of substituted acetophenones with ... more Novel isoxazoline derivatives were synthesized by condensation of substituted acetophenones with aldehyde in presence of alcoholic NaOH to get intermediate chalcones, which were further treated with hydroxylamine hydrochloride in presence of sodium hydroxide to get isoxazoline derivatives. The latter were refluxed separately with isonicotinic acid hydrazide and sulphanilamide in presence of formaldehyde for 6-10 h to afford corresponding Mannich bases. The structures of synthesized compounds were established on the basis of melting point, TLC, IR, 1H NMR and HRMS. Antimycobacterial activity of compounds (3a-j) were assessed against M. tuberculosis (vaccine strain, H37 Rv strain) ATCC27294 using microplate Alamar Blue assay (MABA). Further the derivatives were evaluated for the antibacterial activity against Gram positive bacteria S. aureus (ATCC 9144), S. epidemidis (ATCC12228) and Gram negative bacteria E. coli (ATCC 25922), Klebsiella (ATCC 4352), while antifungal activity against A. flavus (ATCC 9643) and A. niger (ATCC 16404) by using agar well diffusion method using ciprofloxacin and fluconazole as standards, respectively. The results of antimicrobial studies showed that some of the derivatives posses mild to moderate biological activity as compared to standard.
International Journal of Experimental Research and Review
The development and validation of analytical methods are crucial in guaranteeing the precision, d... more The development and validation of analytical methods are crucial in guaranteeing the precision, dependability, and excellence of pharmaceutical analysis. This research investigates the field of pharmaceutical chemistry by doing a comparative examination of analytical techniques for the simultaneous determination of Bilastine and Montelukast Sodium in both bulk and tablet forms. The selected method for this analysis is planar chromatography. The simplicity, specificity, precision, and accuracy of a high-performance liquid chromatography (HPTLC) approach were investigated for their use in the simultaneous estimation of the antihistaminic combination medication Bilastine and Montelukast Sodium in bulk and its pharmaceutical dose for the treatment of allergic rhinitis. Densitometric readings were taken at 254 nm after separating substances using ethyl acetate, toluene, methanol, and ammonia (7:0.5:1.5:0.5v/v/v/v) as mobile phase and precoated aluminium silica gel plates (60F254) as stat...
International Journal of Research in Pharmacy and Chemistry
The present study reveals that novel, simple Analytical Method Ultraviolet Spectrophotometer & Hi... more The present study reveals that novel, simple Analytical Method Ultraviolet Spectrophotometer & High Performance liquid chromatography was developed of for the analysis of Fenbendazole. In the ultraviolet Spectrophotometer of Fenbendazole is detected at 221 nm. The proposed method is fully validated & found to be linear over a workable drug concentration & R 2 was found to be 0.998, accurate, Precise & robust and suitable for routine analysis. HPLC method development was done. HPLC developed method Chromatographic Separation was achieved on the C18 ODS (250-X 4.6MM,5µm) column using Mobile Phase Acetonitrile: Methanol in different ratio (85:15) as mobile phase at a flow rate 0.8ml/minute, 30 min run time & 20µl injection volume with 30°C column temperature is employed for the analysis. The Ultraviolet spectrophotometric method was developed using Schimadzu 1800. The method was validated as per ICH Q2B guideline. This is fast & inexpensive method. It is suitable for research laboratories as well as for quality control analysis in pharmaceutical industries.
JPC - Journal of Planar Chromatography - Modern TLC, 2006
... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78.... more ... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78. [13] MS Raju, DG Sankar, and CS Sastry, Asian J. Chem. ... Sci. 86 (1997) 1030. [24] S. Lakshmi, V. Anilkumar, M. Venkatesan, and TK Raja, Indian Drugs 40(10) (2003) 589591. ...
Haridrakhand is polyherbomineral Ayurvedic formulation employed in skin disorders, allergies, shi... more Haridrakhand is polyherbomineral Ayurvedic formulation employed in skin disorders, allergies, shitapitta, urticaria, and psoriasis. A simple, specific, precise RP-HPLC and HPTLC method for estimation of Curcumin in Haridrakhand tablet formulations were developed and validated as per ICH guidelines Q2(R1). The development was done using precoated TLC plate's lino mat IV sample spotter. Toluene: ethyl acetate: methanol (4: 5: 1 % v / v / v) were used as mobile phase. Measurements were performed at 421 nm in using Server vision cats-server. The developed HPLC method involved use of C18 ODS, (250× 4.6) mm, 5 μ column. Acetonitrile: 0.05% orthophosphoric acid (70:30) was used as mobile phase at 0.7 mL/min flow rate and 394 nm wavelength maxima for detection. The linearity range 50-1000 ng/spot was set for HPTLC and for HPLC 1-25 µg/ml. Rf value and retention time by was found to be 0.421±0.03 and 8.387 by HPTLC and HPLC respectively.
Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality cont... more Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality control aspect of Ayurvedic polyherbal medicines. The modern methods can be used for standardization of these formulations. The qualitative and quantitative study of herbal drug can be accomplished by simple, rapid and cost effective UV Absorption Specrophotometric method. In the present study the Ayurvedic polyherbal formulation used as a brain tonic, was analyzed by a UV specrtophotometric method using shankhpushpin (Evolvoulous alsinoide) and asiaticoside (Centella asiatica) as marker compounds. The validation parameter as per ICH guidelines such as linearity, Precision, accuracy, limit of detection and limit of quantitation was studied. The linearity was observed in the range of 5-30μg/ml for both the markers. Limit of detection and quantitation was observed in nanograms showing high sensitivity of the developed method. The percent recovered amount of was 97.28 % Shankhpushpin and 95.69%...
A simple, accurate, reproducible, rapid and precise high-performance thin-layer chromatographic m... more A simple, accurate, reproducible, rapid and precise high-performance thin-layer chromatographic method has been established and validated for simultaneous determination of Aceclofenac and Paracetamol in bulk and combined tablet dosage form and from biological fluids. Both the drugs were separated on aluminum plates precoated with silica gel 60 F254; Toluene: n-Butanol (7:3v/v) was used as mobile phase. Quantitative analysis was performed by densitometric scanning at 263 nm. The RF values of aceclofenac and paracetamol were 0.14 and 0.43, respectively. The calibration plot was linear over the range of 200–1200 ng/spot for aceclofenac and paracetamol, with correlation coefficients, r, 0.9979 and 0.9995, respectively. Recovery of aceclofenac and paracetamol was 100.14–101.80 and 100.04–100.32%, respectively. The suitability of densitometric TLC for quantitative analysis of these compounds was proved by validation in accordance with the requirements of ICH guidelines. The recovery of ac...
Ayurveda is the oldest surviving complete medical system in the world. Asava and Arishta are uniq... more Ayurveda is the oldest surviving complete medical system in the world. Asava and Arishta are unique dosage form discovered by Ayurveda having long shelf life. Abhayarishta is an important ayurvedic formulation used to relieve the problems like piles and constipation, helpful in mild ascites and dropsy, relieve in urinary obstruction, increase digestive power and is helpful in enlargement of liver, spleen and anemia, which is prepared by fermenting the decoction of specified plant materials using fruits of Terminalia chebula. Abhayarishta a marketed ayurvedic formulation containing Gallic acid as one of the active ingredient. The objective of this study was to determine the level of alcohol, and pH in commercially available Abhayarishta to establish a routine procedure for standardization of these Ayurvedic preparations. In present communication, HPLC and UV method was developed for standardization of Abhayarishta by quantitative estimation of major compound, Gallic acid as marker as...
Three simple, accurate and economical methods have been developed for the estimation of esomepraz... more Three simple, accurate and economical methods have been developed for the estimation of esomeprazole and domperidone in tablet dosage form. First method is based on the simultaneous equations, wavelengths selected for analysis were 301.0 nm (λmax of esomeprazole) and 287.5 nm (λmax of domperidone), respectively, in methanol. Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 291.0 nm (iso-absorptive point) and 301 nm (λmax of esomeprazole). Third method is first order derivative spectroscopy using 301.5 nm (zero cross for esomeprazole) and 286.5 nm (zero cross for domperidone). The linearity was obtained in the concentration range of 530 μg/ml and 4–20 μg/ml for esomeprazole and domperidone, respectively. The results of the analysis have been validated statistically and by recovery studies.
A specific and sensitive high-performance liquid chromatographic method for the simultaneous dete... more A specific and sensitive high-performance liquid chromatographic method for the simultaneous determination of rabeprazole sodium and domperidone in bulk drug and capsules was developed. Ranitidine hydrochloride was used as an internal standard. Separation of the drugs was carried out on the Luna C18 (5μ, 250 mm X 4.60 mm i.d.) at ambient temperature using a mobile phase consisting of ammonium acetate buffer pH 7.4 and acetonitrile (60:40 v/v). Flow rate was 1.0 ml/min with an average operating pressure of 158 kg/cm2. Quantitation was achieved with UV detection at 286 nm based on peak area with linear calibration curves at concentration ranges 10-50 μg/ml and 15-75 μg/ml for rabeprazole and domperidone, respectively. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the capsule excipients was found. The method was validated in terms of precision, robustness, recovery and limit of detection and quantitation.
International journal of drug delivery technology, Dec 24, 2023
Ayurveda is the world's oldest continuous medicinal tradition. Ayurveda's discovery of the long-l... more Ayurveda is the world's oldest continuous medicinal tradition. Ayurveda's discovery of the long-lasting single-dose forms of Asava and Arishta is a boon to modern medicine. Diarrhea, colitis, chronic cough, asthma, tuberculosis, and improved digestive function are only a few of the conditions for which the ayurvedic formulation Babbularishta is utilized. Gallic acid is one of the active ingredients in babbularishta, a commercially available ayurvedic medicine. The purpose of this study was to develop a standard for the quality of commercially available Babbularishta by measuring its alcohol content and pH. To meet World Health Organisation (WHO) standards, an high-performance liquid chromatography (HPLC) and ultraviolet (UV) method was devised for quantifying the main component, gallic acid, in babbularishta. Gas chromatography and redox titrimetry were used to confirm the accuracy of the formulation determined during the development process. The formulation was further tested for pharmacological activity, elemental makeup, pesticide traces, and heavy metal concentration.
Research Journal of Pharmacy and Technology, Dec 28, 2010
Two simple spectrophotometric: methods for the determination of ac:ec:lofenac: and paracetamol in... more Two simple spectrophotometric: methods for the determination of ac:ec:lofenac: and paracetamol in tablets have been developed. First method is based on the additivity or absorbances. Second method is based on the determination or graphical absorbance ratio at two selected wavelengths; one being the isoabsorptive point for the two drugs (230 nm) and the other being the absorption maximum of parac:etamol (256 nm). Beer-Lambert's law is obeyed for both the drugs in the concentration range 1-10 J.Lg/mL. Both the methods were found to be simple, rapid, and accurate and can be adopted in routine analysis of drugs in formulations.
Jpc – Journal Of Planar Chromatography – Modern Tlc, Aug 1, 2006
... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78.... more ... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78. [13] MS Raju, DG Sankar, and CS Sastry, Asian J. Chem. ... Sci. 86 (1997) 1030. [24] S. Lakshmi, V. Anilkumar, M. Venkatesan, and TK Raja, Indian Drugs 40(10) (2003) 589591. ...
International Journal of Health Sciences (IJHS), Jun 11, 2022
A simple, rapid, specific, precise and accurate Reverse phase high performance liquid chromatogra... more A simple, rapid, specific, precise and accurate Reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for quantitative analysis of Ketorolac tromethamine in bulk and marketed formulations such as tablet, ophthalmic solution and gel and validated as per ICH guidelines. Chromatographic separation was performed on a C18 column using an optimized mobile phase of Methanol: Water (75:25) at a flow rate of 1ml/min using the HPLC method. The detection was done at 319 nm and Ketorolac tromethamine had a retention time 2.522 minutes. The total run time of analysis was 10minutes. The drug obeyed Beers law in the concentration range of 4-12µg/ml and the calibration plot revealed a linear relationship. The LOD and LOQ for ketorolac tromethamine were determined to be 0.415 µg/ml and 1.3 µg/ml, respectively. The accuracy and repeatability studies of the proposed method was found to be in acceptable limits, %RSD was less than 2. The proposed method showed excellent linearity, specificity, accuracy, precision, LOD, LOQ and system suitability results within the acceptance criteria. This method with features of less run time and lower retention time can be used for routine analysis of Ketorolac tromethamine in bulk and pharmaceutical dosage forms and does not require any complicated methods.
Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality cont... more Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality control aspect of Ayurvedic polyherbal medicines. The modern methods can be used for standardization of these formulations. The qualitative and quantitative study of herbal drug can be accomplished by simple, rapid and cost effective UV Absorption Specrophotometric method. In the present study the Ayurvedic polyherbal formulation used as a brain tonic, was analyzed by a UV specrtophotometric method using shankhpushpin (Evolvoulous alsinoide) and asiaticoside (Centella asiatica) as marker compounds. The validation parameter as per ICH guidelines such as linearity, Precision, accuracy, limit of detection and limit of quantitation was studied. The linearity was observed in the range of 5-30μg/ml for both the markers. Limit of detection and quantitation was observed in nanograms showing high sensitivity of the developed method. The percent recovered amount of was 97.28 % Shankhpushpin and 95.69% Asiaticoside. The method was robustness and ruggedness for analysis of polyherbal formulation. The polyherbal Ayurvedic formulation can be analyzed successfully by UV Absorption Spectrophotometric method
Novel isoxazoline derivatives were synthesized by condensation of substituted acetophenones with ... more Novel isoxazoline derivatives were synthesized by condensation of substituted acetophenones with aldehyde in presence of alcoholic NaOH to get intermediate chalcones, which were further treated with hydroxylamine hydrochloride in presence of sodium hydroxide to get isoxazoline derivatives. The latter were refluxed separately with isonicotinic acid hydrazide and sulphanilamide in presence of formaldehyde for 6-10 h to afford corresponding Mannich bases. The structures of synthesized compounds were established on the basis of melting point, TLC, IR, 1H NMR and HRMS. Antimycobacterial activity of compounds (3a-j) were assessed against M. tuberculosis (vaccine strain, H37 Rv strain) ATCC27294 using microplate Alamar Blue assay (MABA). Further the derivatives were evaluated for the antibacterial activity against Gram positive bacteria S. aureus (ATCC 9144), S. epidemidis (ATCC12228) and Gram negative bacteria E. coli (ATCC 25922), Klebsiella (ATCC 4352), while antifungal activity against A. flavus (ATCC 9643) and A. niger (ATCC 16404) by using agar well diffusion method using ciprofloxacin and fluconazole as standards, respectively. The results of antimicrobial studies showed that some of the derivatives posses mild to moderate biological activity as compared to standard.
International Journal of Experimental Research and Review
The development and validation of analytical methods are crucial in guaranteeing the precision, d... more The development and validation of analytical methods are crucial in guaranteeing the precision, dependability, and excellence of pharmaceutical analysis. This research investigates the field of pharmaceutical chemistry by doing a comparative examination of analytical techniques for the simultaneous determination of Bilastine and Montelukast Sodium in both bulk and tablet forms. The selected method for this analysis is planar chromatography. The simplicity, specificity, precision, and accuracy of a high-performance liquid chromatography (HPTLC) approach were investigated for their use in the simultaneous estimation of the antihistaminic combination medication Bilastine and Montelukast Sodium in bulk and its pharmaceutical dose for the treatment of allergic rhinitis. Densitometric readings were taken at 254 nm after separating substances using ethyl acetate, toluene, methanol, and ammonia (7:0.5:1.5:0.5v/v/v/v) as mobile phase and precoated aluminium silica gel plates (60F254) as stat...
International Journal of Research in Pharmacy and Chemistry
The present study reveals that novel, simple Analytical Method Ultraviolet Spectrophotometer & Hi... more The present study reveals that novel, simple Analytical Method Ultraviolet Spectrophotometer & High Performance liquid chromatography was developed of for the analysis of Fenbendazole. In the ultraviolet Spectrophotometer of Fenbendazole is detected at 221 nm. The proposed method is fully validated & found to be linear over a workable drug concentration & R 2 was found to be 0.998, accurate, Precise & robust and suitable for routine analysis. HPLC method development was done. HPLC developed method Chromatographic Separation was achieved on the C18 ODS (250-X 4.6MM,5µm) column using Mobile Phase Acetonitrile: Methanol in different ratio (85:15) as mobile phase at a flow rate 0.8ml/minute, 30 min run time & 20µl injection volume with 30°C column temperature is employed for the analysis. The Ultraviolet spectrophotometric method was developed using Schimadzu 1800. The method was validated as per ICH Q2B guideline. This is fast & inexpensive method. It is suitable for research laboratories as well as for quality control analysis in pharmaceutical industries.
JPC - Journal of Planar Chromatography - Modern TLC, 2006
... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78.... more ... Biomed. Appl. (1993) 291297. [12] K. Manoj and S. Anbazhagan, Indian Drugs 41(10) (2004) 78. [13] MS Raju, DG Sankar, and CS Sastry, Asian J. Chem. ... Sci. 86 (1997) 1030. [24] S. Lakshmi, V. Anilkumar, M. Venkatesan, and TK Raja, Indian Drugs 40(10) (2003) 589591. ...
Haridrakhand is polyherbomineral Ayurvedic formulation employed in skin disorders, allergies, shi... more Haridrakhand is polyherbomineral Ayurvedic formulation employed in skin disorders, allergies, shitapitta, urticaria, and psoriasis. A simple, specific, precise RP-HPLC and HPTLC method for estimation of Curcumin in Haridrakhand tablet formulations were developed and validated as per ICH guidelines Q2(R1). The development was done using precoated TLC plate's lino mat IV sample spotter. Toluene: ethyl acetate: methanol (4: 5: 1 % v / v / v) were used as mobile phase. Measurements were performed at 421 nm in using Server vision cats-server. The developed HPLC method involved use of C18 ODS, (250× 4.6) mm, 5 μ column. Acetonitrile: 0.05% orthophosphoric acid (70:30) was used as mobile phase at 0.7 mL/min flow rate and 394 nm wavelength maxima for detection. The linearity range 50-1000 ng/spot was set for HPTLC and for HPLC 1-25 µg/ml. Rf value and retention time by was found to be 0.421±0.03 and 8.387 by HPTLC and HPLC respectively.
Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality cont... more Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality control aspect of Ayurvedic polyherbal medicines. The modern methods can be used for standardization of these formulations. The qualitative and quantitative study of herbal drug can be accomplished by simple, rapid and cost effective UV Absorption Specrophotometric method. In the present study the Ayurvedic polyherbal formulation used as a brain tonic, was analyzed by a UV specrtophotometric method using shankhpushpin (Evolvoulous alsinoide) and asiaticoside (Centella asiatica) as marker compounds. The validation parameter as per ICH guidelines such as linearity, Precision, accuracy, limit of detection and limit of quantitation was studied. The linearity was observed in the range of 5-30μg/ml for both the markers. Limit of detection and quantitation was observed in nanograms showing high sensitivity of the developed method. The percent recovered amount of was 97.28 % Shankhpushpin and 95.69%...
A simple, accurate, reproducible, rapid and precise high-performance thin-layer chromatographic m... more A simple, accurate, reproducible, rapid and precise high-performance thin-layer chromatographic method has been established and validated for simultaneous determination of Aceclofenac and Paracetamol in bulk and combined tablet dosage form and from biological fluids. Both the drugs were separated on aluminum plates precoated with silica gel 60 F254; Toluene: n-Butanol (7:3v/v) was used as mobile phase. Quantitative analysis was performed by densitometric scanning at 263 nm. The RF values of aceclofenac and paracetamol were 0.14 and 0.43, respectively. The calibration plot was linear over the range of 200–1200 ng/spot for aceclofenac and paracetamol, with correlation coefficients, r, 0.9979 and 0.9995, respectively. Recovery of aceclofenac and paracetamol was 100.14–101.80 and 100.04–100.32%, respectively. The suitability of densitometric TLC for quantitative analysis of these compounds was proved by validation in accordance with the requirements of ICH guidelines. The recovery of ac...
Ayurveda is the oldest surviving complete medical system in the world. Asava and Arishta are uniq... more Ayurveda is the oldest surviving complete medical system in the world. Asava and Arishta are unique dosage form discovered by Ayurveda having long shelf life. Abhayarishta is an important ayurvedic formulation used to relieve the problems like piles and constipation, helpful in mild ascites and dropsy, relieve in urinary obstruction, increase digestive power and is helpful in enlargement of liver, spleen and anemia, which is prepared by fermenting the decoction of specified plant materials using fruits of Terminalia chebula. Abhayarishta a marketed ayurvedic formulation containing Gallic acid as one of the active ingredient. The objective of this study was to determine the level of alcohol, and pH in commercially available Abhayarishta to establish a routine procedure for standardization of these Ayurvedic preparations. In present communication, HPLC and UV method was developed for standardization of Abhayarishta by quantitative estimation of major compound, Gallic acid as marker as...
Three simple, accurate and economical methods have been developed for the estimation of esomepraz... more Three simple, accurate and economical methods have been developed for the estimation of esomeprazole and domperidone in tablet dosage form. First method is based on the simultaneous equations, wavelengths selected for analysis were 301.0 nm (λmax of esomeprazole) and 287.5 nm (λmax of domperidone), respectively, in methanol. Second method is Q-analysis method, based on absorbance ratio at two selected wavelengths 291.0 nm (iso-absorptive point) and 301 nm (λmax of esomeprazole). Third method is first order derivative spectroscopy using 301.5 nm (zero cross for esomeprazole) and 286.5 nm (zero cross for domperidone). The linearity was obtained in the concentration range of 530 μg/ml and 4–20 μg/ml for esomeprazole and domperidone, respectively. The results of the analysis have been validated statistically and by recovery studies.
A specific and sensitive high-performance liquid chromatographic method for the simultaneous dete... more A specific and sensitive high-performance liquid chromatographic method for the simultaneous determination of rabeprazole sodium and domperidone in bulk drug and capsules was developed. Ranitidine hydrochloride was used as an internal standard. Separation of the drugs was carried out on the Luna C18 (5μ, 250 mm X 4.60 mm i.d.) at ambient temperature using a mobile phase consisting of ammonium acetate buffer pH 7.4 and acetonitrile (60:40 v/v). Flow rate was 1.0 ml/min with an average operating pressure of 158 kg/cm2. Quantitation was achieved with UV detection at 286 nm based on peak area with linear calibration curves at concentration ranges 10-50 μg/ml and 15-75 μg/ml for rabeprazole and domperidone, respectively. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the capsule excipients was found. The method was validated in terms of precision, robustness, recovery and limit of detection and quantitation.
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