Introduction: Serum endothelin-1 (ET-1) has been observed in sickle cell disease (SCD). The biolo... more Introduction: Serum endothelin-1 (ET-1) has been observed in sickle cell disease (SCD). The biological mechanism of the synthesis of ET-1 remains unclear. The aim of the meta-analysis is to provide an overview of serum ET-1 level changes and to discuss the significance of ET-1 in SCD.
Background: Hb level decreased and Serum Endothelin-1 (ET-) level have been observed increased in... more Background: Hb level decreased and Serum Endothelin-1 (ET-) level have been observed increased in sickle cell disease. The biological mechanism of synthesis and regulation of the Endothelin-1 (ET-) will be clear. The aim of the research study is to provide an overview of the Hb, CBC count, and Sr. Endothelin-1 level changes and its significance in sickle cell disease. Methods: Hematological values Complete blood count (CBC) was measured on fully Automatic blood cell counter hematology analyzer (Coulter LH 780), and HmX Hematology Analyzer with Autoloader. But in this study, we have concentrated on the variables like, Haemoglobin (Hb), WBC, RBC, PCV and MCV count. All the values were compared to the reference values. The ET-1 was determined by using enzyme-immunoassay (ELISA kit) method, specifically designed for direct measurement of plasma ET-1. The sensitivity of the assay is 0.5pg/ml, and the cross-sensitivity of the antibodies used is reported to be < 1% with big ET-1. Result...
Introduction: Diabetes Mellitus is one of the diseases with many complications which requirea nov... more Introduction: Diabetes Mellitus is one of the diseases with many complications which requirea novel biomarker for early diagnosis and treatment. Asymmetrical dimethylarginine (ADMA)an endothelial dysfunction biomarker as proven to be a promising biomarker in type 2 diabetes mellitus (T2DM). Method:-Data extraction was conducted independently using a standardized data extraction form. Only full length text articles were included in the study. For each included article, we extracted information on the title, authors, publication year, name of the study, sample size, number of diabetes cases and control study, mean (standard deviation) for the ADMA level, assay for measuring ADMA levels, and statistical methods used for the analysis. Observations and results: Level of serum ADMA were highly significantly elevated in patients T2DM comparedto healthy control.(Standardmeandeference [SMD]: +0.58, CI:+0.55 to +0.61). The result of each study also showed that serum ADMA in T2DM group was hig...
International Journal of Clinical Biochemistry and Research, 2019
Introduction: Diabetes mellitus (DM) is associated with an increased risk of cardiovascular disea... more Introduction: Diabetes mellitus (DM) is associated with an increased risk of cardiovascular disease (CVD) and mortality. Impaired glucose tolerance (IGT) and impaired fasting glucose (IFG) are well characterized prediabetes conditions and about half of those proceed with the development of type 2 diabetes. During the pre-diabetic state, the risk of a CVD is modestly increased. The aim of present study is to evaluate serum Homocysteine (Hyc) level in prediabetes and type 2 diabetes patients and find the association between Hyc and other variables. Materials and Methods: This cross sectional descriptive study was conducted on 450 participants. Out of this 150 were type 2 diabetic patients, 150 prediabetes and 150 controls. Homocystine and biochemical parameters were measured. Results: Homocysteine is a sulfur-containing amino acid formed during the metabolism of methionine consider as a an early detection marker in cardiovascular disease elevated in prediabetes patients, type 2 diabetes compared to controls. Conclusion: We observed that homocysteine significantly increased in prediabetes compared to healthy control. Homocysteine levels can indicate the risk of CVD in prediabetic stage. Prediabetes people are under risk of CAD and type 2 diabetes. The evaluation of the Homocysteine levels may improve the early diagnosis of CVD in prediabetes patients.
Diabetes Mellitus is one of the diseases with many complications which require a novel biomarker ... more Diabetes Mellitus is one of the diseases with many complications which require a novel biomarker for early diagnosis and treatment. 8-hydroxy deoxyguanine (8-OHdG), DNA damaging oxidative stress biomarker as proven to be a promising biomarker in type 2 diabetes mellitus (T2DM). Method: Data extraction was conducted independently using a standardized data extraction form. Only full length text articles were included in the study. For each included article, we extracted information on the title, authors, publication year, name of the study, sample size, number of diabetes cases and control study, mean (standard deviation) for the 8-HdG level, assay for measuring 8-HdG levels, and statistical methods used for the analysis. Observations and results: Level of serum 8-HdG were highly significantly elevated in patients T2DM compared to healthy control. (Standard mean deference [SMD]) and (95% Confidence Interval [CI] ) +1051.06 (+996.29 to +1135.83). The result of each study also showed th...
Introduction: Serum endothelin-1 (ET-1) has been observed in sickle cell disease (SCD). The biolo... more Introduction: Serum endothelin-1 (ET-1) has been observed in sickle cell disease (SCD). The biological mechanism of the synthesis of ET-1 remains unclear. The aim of the meta-analysis is to provide an overview of serum ET-1 level changes and to discuss the significance of ET-1 in SCD.
Background: Hb level decreased and Serum Endothelin-1 (ET-) level have been observed increased in... more Background: Hb level decreased and Serum Endothelin-1 (ET-) level have been observed increased in sickle cell disease. The biological mechanism of synthesis and regulation of the Endothelin-1 (ET-) will be clear. The aim of the research study is to provide an overview of the Hb, CBC count, and Sr. Endothelin-1 level changes and its significance in sickle cell disease. Methods: Hematological values Complete blood count (CBC) was measured on fully Automatic blood cell counter hematology analyzer (Coulter LH 780), and HmX Hematology Analyzer with Autoloader. But in this study, we have concentrated on the variables like, Haemoglobin (Hb), WBC, RBC, PCV and MCV count. All the values were compared to the reference values. The ET-1 was determined by using enzyme-immunoassay (ELISA kit) method, specifically designed for direct measurement of plasma ET-1. The sensitivity of the assay is 0.5pg/ml, and the cross-sensitivity of the antibodies used is reported to be < 1% with big ET-1. Result...
Introduction: Diabetes Mellitus is one of the diseases with many complications which requirea nov... more Introduction: Diabetes Mellitus is one of the diseases with many complications which requirea novel biomarker for early diagnosis and treatment. Asymmetrical dimethylarginine (ADMA)an endothelial dysfunction biomarker as proven to be a promising biomarker in type 2 diabetes mellitus (T2DM). Method:-Data extraction was conducted independently using a standardized data extraction form. Only full length text articles were included in the study. For each included article, we extracted information on the title, authors, publication year, name of the study, sample size, number of diabetes cases and control study, mean (standard deviation) for the ADMA level, assay for measuring ADMA levels, and statistical methods used for the analysis. Observations and results: Level of serum ADMA were highly significantly elevated in patients T2DM comparedto healthy control.(Standardmeandeference [SMD]: +0.58, CI:+0.55 to +0.61). The result of each study also showed that serum ADMA in T2DM group was hig...
International Journal of Clinical Biochemistry and Research, 2019
Introduction: Diabetes mellitus (DM) is associated with an increased risk of cardiovascular disea... more Introduction: Diabetes mellitus (DM) is associated with an increased risk of cardiovascular disease (CVD) and mortality. Impaired glucose tolerance (IGT) and impaired fasting glucose (IFG) are well characterized prediabetes conditions and about half of those proceed with the development of type 2 diabetes. During the pre-diabetic state, the risk of a CVD is modestly increased. The aim of present study is to evaluate serum Homocysteine (Hyc) level in prediabetes and type 2 diabetes patients and find the association between Hyc and other variables. Materials and Methods: This cross sectional descriptive study was conducted on 450 participants. Out of this 150 were type 2 diabetic patients, 150 prediabetes and 150 controls. Homocystine and biochemical parameters were measured. Results: Homocysteine is a sulfur-containing amino acid formed during the metabolism of methionine consider as a an early detection marker in cardiovascular disease elevated in prediabetes patients, type 2 diabetes compared to controls. Conclusion: We observed that homocysteine significantly increased in prediabetes compared to healthy control. Homocysteine levels can indicate the risk of CVD in prediabetic stage. Prediabetes people are under risk of CAD and type 2 diabetes. The evaluation of the Homocysteine levels may improve the early diagnosis of CVD in prediabetes patients.
Diabetes Mellitus is one of the diseases with many complications which require a novel biomarker ... more Diabetes Mellitus is one of the diseases with many complications which require a novel biomarker for early diagnosis and treatment. 8-hydroxy deoxyguanine (8-OHdG), DNA damaging oxidative stress biomarker as proven to be a promising biomarker in type 2 diabetes mellitus (T2DM). Method: Data extraction was conducted independently using a standardized data extraction form. Only full length text articles were included in the study. For each included article, we extracted information on the title, authors, publication year, name of the study, sample size, number of diabetes cases and control study, mean (standard deviation) for the 8-HdG level, assay for measuring 8-HdG levels, and statistical methods used for the analysis. Observations and results: Level of serum 8-HdG were highly significantly elevated in patients T2DM compared to healthy control. (Standard mean deference [SMD]) and (95% Confidence Interval [CI] ) +1051.06 (+996.29 to +1135.83). The result of each study also showed th...
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