Purpose. To investigate the possibility that cell death in retinal detachment may occur by reacti... more Purpose. To investigate the possibility that cell death in retinal detachment may occur by reactivation of apoptotic programmed cell death mechanisms. Methods. Unilateral retinal detachments were created in adult cats using 0.25% sodium hyaluronate; detached and control retinas were studied at different intervals. Internucleosomal DNA fragmentation (one of the landmarks of apoptosis) was investigated in tissue sections with the TUNEL technique, which uses terminal transferase to label with biotinylated nucleotides the 3' ends of DNA fragments. Sections also were labeled with propidium iodide, which intensely stains pyknotic nuclei. In addition, one time point was selected for analysis with electron microscopy.
Hasta este punto, la historia ha estado señalando que la realidad educativa • que ponga el acento... more Hasta este punto, la historia ha estado señalando que la realidad educativa • que ponga el acento de la actividad educativa, centrándose más en el pro-
Purpose. To investigate the possibility that cell death in retinal detachment may occur by reacti... more Purpose. To investigate the possibility that cell death in retinal detachment may occur by reactivation of apoptotic programmed cell death mechanisms. Methods. Unilateral retinal detachments were created in adult cats using 0.25% sodium hyaluronate; detached and control retinas were studied at different intervals. Internucleosomal DNA fragmentation (one of the landmarks of apoptosis) was investigated in tissue sections with the TUNEL technique, which uses terminal transferase to label with biotinylated nucleotides the 3' ends of DNA fragments. Sections also were labeled with propidium iodide, which intensely stains pyknotic nuclei. In addition, one time point was selected for analysis with electron microscopy.
Hasta este punto, la historia ha estado señalando que la realidad educativa • que ponga el acento... more Hasta este punto, la historia ha estado señalando que la realidad educativa • que ponga el acento de la actividad educativa, centrándose más en el pro-
Uploads
Papers by Ruben oñate