Papers by Reyhane Ebrahimi
BMC Endocrine Disorders
Objective A better understanding of mechanisms regulating lipogenesis and adipogenesis is needed ... more Objective A better understanding of mechanisms regulating lipogenesis and adipogenesis is needed to overcome the obesity pandemic. We aimed to study the relationship of the transcript levels of peroxisome proliferator activator receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBP-α), liver X receptor (LXR), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) from obese and normal-weight women with a variety of anthropometric indices, metabolic and biochemical parameters, and insulin resistance. Methods Real‐time PCR was done to evaluate the transcript levels of the above‐mentioned genes in VAT and SAT from all participants. Results Using principal component analysis (PCA) results, two significant principal components were identified for adipogenic and lipogenic genes in SAT (SPC1 and SPC2) and VAT (VPC1 and VPC2). SPC1 was characterized b...
Journal of Cellular Physiology
Journal of Cellular Physiology
Noncoding RNAs (ncRNAs) are important regulators of gene expression in different cell processes. ... more Noncoding RNAs (ncRNAs) are important regulators of gene expression in different cell processes. Due to their ability in monitoring neural development genes, these transcripts confer neurons with the potential to exert broad control over the expression of genes for performing neurobiological functions. Although the change of ncRNA expression in different neurodegenerative diseases has been reviewed elsewhere, only recent evidence drove our attention to unravel the involvement of these molecules in neuroinflammation within these devastating disorders. Remarkably, the interactions between ncRNAs and inflammatory pathways are not fully recognized. Therefore, this review has focused on the interplay between diverse inflammatory pathways and the related ncRNAs, including microRNAs, long noncoding RNAs, and competing endogenous RNAs in Alzheimer's disease, Parkinson's diseases, amyotrophic lateral sclerosis, epilepsy, multiple sclerosis, Huntington's disease, and prion diseases. Providing novel insights in the field of combining biomarkers is a critical step for using them as diagnostic tools and therapeutic targets in clinical settings.
Journal of Diabetes & Metabolic Disorders
Introduction The rising prevalence of diabetes shows high health and socioeconomic burdens. There... more Introduction The rising prevalence of diabetes shows high health and socioeconomic burdens. Therefore, the development and evaluation of new diagnostic methods may improve the detection of disease and its complications in the early stages. This study aimed to analyze the scope of the studies related to diabetes diagnosis. Material and method Publications from January 2015 until December 2019 (5 years) were searched with keywords of (diabetes OR diabetic) AND (Iran) in Scopus and PubMed databases. All data were reviewed by two reviewers and the included publications were categorized based on the subjects, study design, and publication year. Results Based on the selected criteria, 103 articles were included. The highest number of publications was observed in 2019. The trend of publication was slightly increased during the study period (2015-2019). Case-control and cross-sectional studies were the most common type of study design used in the included documents. Publications in the field of diagnostic models, biomarkers, and biosensors from 2015 to 2019 showed an increasing trend compared to others subjects. Discussion and conclusion Studies about proper diabetes diagnostic procedures such as new diagnostic techniques, using diagnostic models, and evaluation of new diagnostic biomarkers in Iran are remarkably increased. However, more original and review studies are needed to improve scientific methods in the field of early detection of diabetes.
Additional file 1: Table S1. Forward and reverse primers used for real-time PCR.
Journal of Physiology and Biochemistry, 2022
There is evidence regarding the association of hyperuricemia with inflammatory disorders. Hence, ... more There is evidence regarding the association of hyperuricemia with inflammatory disorders. Hence, it has been of particular interest to dissect the exact role of alteration in uric acid (UA) levels in the context of inflammation. Recently, the endoplasmic reticulum (ER) stress pathway has come into the forefront as a possible mechanism linking hyperuricemia to inflammation. Here, we intended to examine the role of UA in the presence or absence of a second stimulus, LPS, in human peripheral blood mononuclear cells (PBMCs), and analyzed ROS production as well as expression of ER stress markers: GRP78 and CHOP, and inflammatory cytokines. PBMCs were isolated using Ficoll gradient centrifugation from healthy volunteers. Cell viability was measured by MTT assay. PBMCs were treated with an increasing concentration of soluble UA (0, 5, 12, and 20 mg/dl) for 20 h, followed by the addition of 100 ng/mL of LPS or vehicle for another 4 h. Real time-PCR was performed to investigate the mRNA expression of GRP78, CHOP, TNF-α, IL-1β, and IL-6, and western blot was used to investigate the protein levels of GRP78 and CHOP. Moreover, ELISA was used to evaluate the protein levels of TNF-α, IL-1β, and IL-6. Finally, intracellular ROS production was determined using fluorescent probes (DCFH-DA). High concentrations of UA either alone or combined with LPS increased the protein levels of GRP78 and CHOP. On the other hand, LPS alone increased the protein levels of GRP78 and CHOP. However, there was no significant difference between the mRNA expression of GRP78, CHOP, TNF-α, IL-1β, and IL-6 when PBMCs were treated with UA. High concentrations of UA augmented LPS-stimulated IL-1β transcript and protein levels as well as TNF-α protein levels in PBMC culture. Moreover, high concentrations of UA along with LPS significantly increased intracellular ROS production. It seems that a high concentration of UA not only induces the protein levels of ER stress markers in PBMCs but also augments the impact of LPS on the levels of pro-inflammatory markers and ROS production.
Medical laboratory sciences, 2020
Background: Several studies suggested that beta-actin, glyceraldehyde-3-phosphate dehydrogenase (... more Background: Several studies suggested that beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and 18S rRNA are expressed constitutively and contribute to the fundamental reference actions essential for cell viability and maintenance. However, there are inconsistency in this regard. Hence, we aimed to evaluate the accuracy of these three potential reference genes for Real‐Time quantitative reverse transcriptase‐polymerase chain reaction (qRT‐PCR) application for normalization in two types of human adipose tissues. Materials and Methods: Subcutaneous and visceral fat tissues were derived from 19 healthy and 20 obese subjects and RT-qPCR was applied to determine the expression levels of beta-actin, GAPDH, and18S rRNA. Results: The gene expression level of beta-actin, GAPDH, and 18S rRNA was essentially the same in the subcutaneous and visceral fat tissues of all participants (P>0.05). Hence, all considered housekeeping genes displayed high expression stability and the ana...
Medical laboratory sciences, 2019
Background : Nonalcoholic fatty liver diseases (NAFLD) is one of the main chronic liver diseases ... more Background : Nonalcoholic fatty liver diseases (NAFLD) is one of the main chronic liver diseases and raises the risk of morbidity and mortality due to its inevitable outcomes. Understanding the clinical manifestations of the liver is critical to identify NAFLD patients with the greatest risk of developing nonalcoholic steatohepatitis and cirrhosis. In the liver, C1q/TNF-related protein 1 (CTRP1) modulates both glucose and lipid metabolism and improves insulin sensitivity which may affect the pathologies of the liver. Materials and Methods : This study was conducted on 22 patients with NAFLD confirmed by ultrasonography and 21 healthy subjects. Clinical and histological variables were analyzed. The ultrasonography procedure was used to quantity Common bile duct (CBD). Liver stiffness (LS) was measured by transient elastography. Res ults : There was a significant difference in CTRP1 levels between NAFLD patients and controls (p=0.032). Moreover, there was a significant positive correl...
Medical laboratory sciences, 2019
Background: Obesity with a rapid grow in developed and developing countries has a close associati... more Background: Obesity with a rapid grow in developed and developing countries has a close association with higher disposition to related diseases such as hypertension. Intracellular functions of sodium, potassium, calcium, phosphate, and iron have been an interested subject in obese patients since their dysregulations are linked to a higher risk of hypertension and other metabolic disorders. Materials and Methods: In this study, the circulating levels of sodium, potassium, calcium, phosphate, and iron were determined in the serum of obese patients compared to normal-weight people. Moreover, we examined the correlation of such electrolytes with the well-known indices of obesity such as body mass index (BMI), waist circumference (WC), hip, triglycerides (TG), cholesterol and other characterizations. Results : The mean levels of sodium, potassium, calcium, phosphate, and iron were significantly different (p < 0.05) in obese patients compared to normal-weight subjects. We observed a po...
Eating and Weight Disorders - Studies on Anorexia, Bulimia and Obesity, 2021
Nutrient patterns have been associated with an increased risk for chronic disease. Evidence to co... more Nutrient patterns have been associated with an increased risk for chronic disease. Evidence to confirm a direct relationship between nutrient patterns and obesity and metabolic syndrome (MetS) throughout population-based differences including cultural contexts add complexity is not well established yet. The aim of this study is to investigate the association between nutrient patterns and MetS among overweight and obese Iranian women. Three hundred and sixty obese and overweight women (25 < BMI < 40) were included in this cross-sectional analysis. Dietary intake of 19 nutrients was evaluated by a semi-quantitative standard food frequency questionnaire (FFQ). MetS was determined by abdominal obesity > 88 (cm) in females, Triglycerides ≥ 150 (mg/dL), dyslipidemia (HDL < 50 mg/dL), systolic blood pressure > 130/85 (millimeters), and glucose > 100 (mg/dL). Body composition was assessed by a multi-frequency bioelectrical impedance analyzer, InBody 770 scanner. Principle components analysis was applied and four nutrient patterns were identified as following: Pattern 1 (thiamin, iron, carbohydrate, zinc, niacin, protein, magnesium, phosphorus, riboflavin), represented the carbo-vitamin group. Lipid group was showed in pattern 2 (PUFAs, MUFA, vitamin E, trans fatty acids, and Pattern 3 (beta-carotene, vitamin K, vitamin A, vitamin C) represented the anti-oxidant group, finally Pattern 4 was the indicator of the milk group (vitamin D, calcium). A significant positive association was observed between the anti-oxidant group and obesity (OR 1.40; 95% CI 1.09–1.8; P = 0.01). No relationship between other nutrient pattern and MetS was observed. The nutrient patterns that are highly loading of beta-carotene, vitamin K, vitamin A, and vitamin C in nutrient patterns may be associated to higher risk of obesity in overweight and obese Iranian women. Level V, cross-sectional descriptive study.
Current Medicinal Chemistry, 2021
Neurological disorders (NDs) comprise a broad range of diseases affecting both central and periph... more Neurological disorders (NDs) comprise a broad range of diseases affecting both central and peripheral nervous systems. These complex multifactorial diseases have a high rate of mortality all over the world, particularly in aged people. Today, new evidence drove our attention to the notable role of noncoding RNAs (ncRNAs) in the progression of NDs. Remarkably, recent studies showed that there are close communication networks among RNA transcripts such as mRNAs, long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and pseudogenes for regulating each other’s expression through competing for shared sequences in microRNAs (miRs). This concept is a new area of ongoing research recognized as competing endogenous RNA (ceRNA) hypothesis. CeRNAs are novel regulatory molecules in a wide range of biological stages and pathological contexts. Indeed, the disruption of ceRNA networks (ceRNETs) may affect neural development genes and induce neuropathological changes leading to the development ...
Diabetology & Metabolic Syndrome, 2020
Background Recent studies point toward the possible regulatory roles of two lncRNAs; metastasis-a... more Background Recent studies point toward the possible regulatory roles of two lncRNAs; metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and taurine upregulated gene 1 (TUG1) in the pathogenesis of obesity-related disorders and regulation of lipogenesis and adipogenesis. In an attempt to understand the molecules involved in human obesity pathogenesis, we aimed to evaluate the expression of MALAT1 and TUG1 in visceral adipose tissues (VAT) and subcutaneous adipose tissues (SAT) of obese women, as compared to normal-weight women. The mRNA expression of possible target genes including peroxisome proliferator-activated receptor gamma (PPARγ), PPARγ coactivator-1 alpha (PGC1α), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) which are involved in adipogenesis and lipogenesis were also examined. Methods This study was conducted on 20 obese [body mass index (BMI) ≥ 30 kg/m 2] female participants and 19 normal-...
Gene Reports, 2020
Please cite this article as: Z. Afshoon, A. Bahiraee, F. Esmaieli, et al., Study of the relations... more Please cite this article as: Z. Afshoon, A. Bahiraee, F. Esmaieli, et al., Study of the relationship between endothelial lipase gene polymorphism and serum levels of HDL-C, Apo A-I and severity of stenosis in non-diabetic coronary artery disease patients, Gene Reports (2020),
Life Sciences, 2020
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Phytotherapy Research, 2020
Over the last few decades, using natural products has been increased to treat different diseases.... more Over the last few decades, using natural products has been increased to treat different diseases. Today, great attention has been pointed toward the usage of natural products such as flavonoids, especially Quercetin (QUR), in the treatment of diseases. QUR as a natural antioxidant has been traditionally used to prevent or treat a variety of diseases such as cancer, cardiovascular disease, polycystic ovary syndrome (PCOS), obesity, chronic inflammation, and reproductive system dysfunction. Several studies demonstrated that QUR acts as an anti-inflammatory, anti-apoptotic, antioxidant, and anticancer agent. With this in view, in this study, we intended to describe an overview of the biological effects of QUR on the ovary. QUR improves the quality of oocytes and embryos. It affects the proliferation and apoptosis and decreases the oxidative stress in granulosa cells (GCs). Furthermore, QUR can be used as a complementary and alternative therapy in ovarian cancer and it has beneficial effects in the treatment of PCOS patients. It seems that QUR as a supplementary factor has different activities for the treatment of different disorders and it also has bidirectional activities. However, further investigations are needed for understanding the efficacy of QUR in the treatment and improvement of gynecological patients.
Background Due to lack of enough date to confirm the association of obesity and depression in mid... more Background Due to lack of enough date to confirm the association of obesity and depression in middle east, We aimed to explore the possible mediatory role of adipokines such as galactin1, transforming growth factor beta (TGF-beta), and endothelial plasminogen activator inhibitor (PAI-1) in the association between obesity and depression.Methods A total of 256 women ranged between 18-48 years old were grouped based on their low carbohydrate diet (LCD) score. Body composition and dietary intake were assessed. Enzyme-linked immunosorbent assay was used to measure serum adipokines levels.Results There was a negative association between LCD score as a covariant and depression as an independent variable (P= 0.02) (beta ±SE= -0.143±0.031) (CI= -0.129 to -0.08). A regression model linear analysis using galactin1, TGF-β, and PAI 1 as a covariant indicated the mediatory effects of these adipokines (P > 0.05). The higher adherence to LCD was associated with a decrease of anthropometric compo...
Meta Gene, 2020
Abstract Background Premature coronary artery disease (pCAD) is a heterogeneous disease affected ... more Abstract Background Premature coronary artery disease (pCAD) is a heterogeneous disease affected by both genetic and environmental determinants. Cytochrome P450 2D6 (CYP2D6) enzyme plays an important role in the oxidation of drugs and inactivation of reactive species. Therefore, polymorphisms in CYP2D6 may lead to an increased susceptibility to pCAD. Here, we analyzed the association between C100T and G1846A polymorphisms of CYP2D6 gene with pCAD in a south Iranian population. Methods Men under 45 and women under 55 years old enrolled in this study as 168 pCAD patients and 167 controls. Clinical characteristics of participants were assessed thorough clinical examination. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was applied for C100T and G1846A polymorphisms. Results The results indicated that the history of smoking and hypertension were significantly associated with the incidence of pCAD (p Conclusion This study demonstrated no significant association of C100T and G1846A polymorphisms of CYP2D6 with pCAD in the south Iranian population. The possible explanation may be attributed to the differences in dissimilar ethnic, genetic, and environmental backgrounds, and also different sample sizes.
The Natural Products Journal, 2019
Background: Up-regulation of matrix metalloproteinase 9 (MMP-9) along with imbalance in ratio of ... more Background: Up-regulation of matrix metalloproteinase 9 (MMP-9) along with imbalance in ratio of MMP-9 relative to tissue inhibitor of metalloproteinase 1 (TIMP-1) is important in the pathogenesis of rheumatoid arthritis (RA). Here, we investigated whether hydroalcoholic extract from root of Alhagi camelorum Fisch can affect the levels of MMP-9 and TIMP-1 in peripheral blood mononuclear cells (PBMCs) of RA patients. Objective: In the current study, we suggest that Alhagi may have an inhibitory effect on MMP-9 production, which is mainly responsible for joint destruction in RA. In addition, we would like to stress that our findings, along with others, can provide the basis for future studies, which might be able to declare the role of chemical ingredients of Alhagi as therapeutic targets for RA treatment. Method: PBMCs were isolated from 12 RA patients and 12 healthy subjects and treated with two concentrations of Alhagi extract (100 and 500 µg/ml) for 24 h. MMP-9 expression and acti...
Critical Reviews in Clinical Laboratory Sciences, 2020
Insulin resistance is associated with an increased risk of several metabolic disorders including ... more Insulin resistance is associated with an increased risk of several metabolic disorders including type 2 diabetes, hypertension and cardiovascular diseases. Advances over the last decade have expanded our understanding of the molecular mechanisms underlying insulin resistance; however, many details of the mechanisms causing insulin resistance remain unknown. Recently, attention has shifted toward the role of epigenetics in insulin resistance. In this regard, acetylation of the histone tails has been widely investigated for its role in influencing both metabolic and mitogenic cascades of insulin signaling. More specifically, histone acetyltransferases and histone deacetylases, as major modulators of chromatin accessibility and gene expression, have been studied to determine a possible interconnectivity between the special effects of lysine acetylation status and tyrosine phosphorylation networks on the target proteins of downstream pathways involved in both metabolic and mitogenic cascades of insulin signaling. There is accumulating evidence for the post-translational modification effects of IGFR, InsR, IRS1/2, PI3K, Akt, GLUT4, FoxO, PGC-1a, PPAR, AMPK and MAPKs on insulin resistance and glucose homeostasis. In this paper, we review the importance of acetylation of these factors in the regulation of insulin signaling and glucose metabolism, with a primary focus on the target proteins of downstream signaling of insulin. We also provide an update on the interplay between epigenetic modification and the cellular genome in the context of insulin signaling and describe the possible effect of the environment on this epigenetic regulation.
Archives of Physiology and Biochemistry, 2019
We aimed to study the correlation of adiponectin level with insulin resistance (IR), carotid inti... more We aimed to study the correlation of adiponectin level with insulin resistance (IR), carotid intimamedia thickness (cIMT), and various obesity indices especially visceral adipose tissue (VAT) thickness, and visceral adiposity index (VAI), in patients with NAFLD (n ¼ 41), T2D (n ¼ 22), NAFLD þ T2D (n ¼ 41), and healthy subjects (n ¼ 20). Results showed the median level of adiponectin in patients with NAFLD (2.97 lg/mL) and ones with NAFLD þ T2D (3.21 lg/mL) is significantly lower rather than in controls (4.39 lg/mL). Moreover, VAI is the only predictor for adiponectin concentration in the combination of patient groups and also in all participants independent of IR and other obesity indices. Adiponectin level had also a positive correlation with cIMT and IR in NAFLD patients. Interestingly, lower level of adiponectin was associated with the presence of T2D, NAFLD, and NAFLD þ T2D independent of IR and obesity indices. Collectively, it seems that VAI reflecting visceral adipose tissue function is a possible predictor of adiponectin level.
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Papers by Reyhane Ebrahimi