Papers by Resti Yudhawati
International Journal of Research Publications
At the end of 2019, an infectious disease was discovered, has spread rapidly throughout the world... more At the end of 2019, an infectious disease was discovered, has spread rapidly throughout the world, and has become a pandemic called COVID-19. Several risk factors for COVID-19 infection are advanced age and comorbid diseases. However, at the beginning of the pandemic in Wuhan, China there were more patients with blood type A who were confirmed positive than other blood types. The study aimed to analyze the relationship between ABO blood type and the severity and characteristics of COVID-19 patients at
International Journal of Research Publications
COVID-19 infection in children is generally asymptomatic or with symptoms but milder than in adul... more COVID-19 infection in children is generally asymptomatic or with symptoms but milder than in adults, although symptoms of severe infection and death may also occur. Cancer patients have a high level of immunosuppression due to the anticancer therapy they are undergoing so that COVID-19 infection is more susceptible to complications. However, until now there have not been many reported cases of COVID-19 in pediatric patients with cancer, so it is not yet known whether there is a relationship between clinical and laboratory manifestations on the degree of severity and patient outcome. This study is an analytic observational study using a retrospective design, based on secondary data. Sampling was done by total sampling technique. Relationship analysis is used to answer the research hypothesis. To find out the relationship between variables, a chi-square and Fisher's exact test was used because the data taken was nominal. Based on the inclusion and exclusion criteria, a total of 36 samples were found to fulfill. The most age range in patients was 3-5 years (36.11%) and was dominated by male sex (63.88%). From 36 samples, 21 of them had blood cancer, with the most type being ALL (90.47%). The degree of severity with the highest rate was moderate (37.83%) and most of the patient outcomes were alive (75%). The analysis carried out stated that there was no relationship between clinical manifestations and the severity of the patient. However, a relationship was found between shortness of breath symptoms and patient outcomes. Meanwhile, in laboratory manifestations there was no relationship between the degree of severity and the patient's outcome. In conclusion, there is a relationship between symptoms of shortness of breath and the outcome of pediatric cancer patients with COVID-19 infection.
Research Journal of Pharmacy and Technology
The aim of the research is to analyze the differences in the subset of T lymphocytes and NK cells... more The aim of the research is to analyze the differences in the subset of T lymphocytes and NK cells at various degrees of disease severity in order to be used in stratification of patients’ management and to predict outcomes for optimal treatment. The study sample of 123 patients with confirmed COVID-19 was classified based on the degree of severity: 50 patients with mild severity, 34 patients with moderate severity and 39 patients with severe to critical severity who were subjected to complete blood count and T lymphocyte subsets (CD3, CD4, CD8) and NK cells with Flowcytometry. There were significant differences in the number of CD 3 cells (p=0.000), CD4 (p=0.000), CD8 (p=0.000), and NK cells (p=0.000) in the three groups. In the severe to critical group there was a decrease in lymphocytes accompanied by decrease of the number of CD3, CD4, CD8 and NK cells as well as an increase in WBC and neutrophils. Based on the outcome, there were significant differences in the number of CD 3 cel...
Journal Of Advanced Pharmacy Education And Research, 2021
Journal Of Advanced Pharmacy Education And Research, 2021
This is an open access journal, and articles are distributed under the terms of the Creative Comm... more This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-Non Commercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Coronary Artery Disease, 2012
Objectives In humans, a secreted isoform of (soluble) receptor for advanced glycation end-product... more Objectives In humans, a secreted isoform of (soluble) receptor for advanced glycation end-products (sRAGE) may act as a decoy receptor of advanced glycation endproduct. The level of sRAGE may reflect the activity of cell surface receptor for advanced glycation end-product. But there has been no study that has demonstrated the association of sRAGE with central aortic stiffness. Here, we studied the relation of plasma sRAGE level and arterial pulse wave velocity in hypertensive patients. Materials and methods A total of 415 patients were enrolled (men; 57.6%, mean age; 53.2 ± 10.8 years), with 25.8% (n = 107) of these being diabetic. All patients underwent pulse wave velocity (PWV) and blood sampling of sRAGE, high-sensitive C-reactive protein, and other serologic markers. Results The log-transformed sRAGE was significantly correlated with the marker of central aortic stiffness heart to femoral PWV (hfPWV; r = 0.165, P = 0.001). It also showed a significant correlation with hfPWV in patients with diabetes (r = 0.301, P = 0.002), but not in patients without diabetes (r = 0.115, P = 0.055). By multiple linear regression analysis, the log-transformed sRAGE was independently correlated with hfPWV (b = 0.13, P = 0.004) when controlled for other variables. Conclusions This study has demonstrated, for the first time, that serum sRAGE level was independently correlated with a marker of central aortic stiffness. This result suggests the potential role of RAGE in the pathogenesis of aortic stiffness. Coron Artery Dis 23:85-90
Archives of Virology, 2012
An H5N1 highly pathogenic avian influenza virus was isolated from conjunctiva of a whooper swan w... more An H5N1 highly pathogenic avian influenza virus was isolated from conjunctiva of a whooper swan with neurological signs, which was captured during the latest H5N1 HPAI outbreak in Japan. The conjunctival swab contained a larger amount of the virus in comparison with the tracheal swab. This is the first report on H5N1 virus isolation from the conjunctiva of a wild bird, and the result may suggest the conjunctival swab to be a critical sample for H5N1 HPAIV detection in waterfowl. Phylogenetic analysis of the HA gene indicated that the virus falls into H5N1 clade 2.3.2.1.
International Journal of Molecular Sciences
Acute respiratory distress syndrome is an acute respiratory failure caused by cytokine storms; hi... more Acute respiratory distress syndrome is an acute respiratory failure caused by cytokine storms; highly pathogenic influenza A virus infection can induce cytokine storms. The innate immune response is vital in this cytokine storm, acting by activating the transcription factor NF-κB. Tissue injury releases a danger-associated molecular pattern that provides positive feedback for NF-κB activation. Exogenous mesenchymal stem cells can also modulate immune responses by producing potent immunosuppressive substances, such as prostaglandin E2. Prostaglandin E2 is a critical mediator that regulates various physiological and pathological processes through autocrine or paracrine mechanisms. Activation of prostaglandin E2 results in the accumulation of unphosphorylated β-catenin in the cytoplasm, which subsequently reaches the nucleus to inhibit the transcription factor NF-κB. The inhibition of NF-κB by β-catenin is a mechanism that reduces inflammation.
International Journal of General Medicine
Background: Coronavirus disease-19 (COVID-19) can, in severe cases, lead to cytokine-release synd... more Background: Coronavirus disease-19 (COVID-19) can, in severe cases, lead to cytokine-release syndrome owing to an excessive immune response. The release of different cytokines aggravates disease severity. IL-1β is a pro-inflammatory cytokine, while IL-10 is an anti-inflammatory cytokine, and both are involved in the human immune response to infection. This study aimed to determine whether serum levels of IL-1β and IL-10 and the ratio of the two over time in patients with COVID-19 could facilitate early identification of disease severity. Methods: An analytical, observational time-series design was employed. Fifty participants were enrolled between May and October 2020 and were divided into two groups-non-severe (n = 20), and severe (n = 30). IL-1β and IL-10 were analyzed using BD cytometric bead array sets. Association of the IL-1β:IL-10 ratio with COVID-19 severity was analyzed using a Mann-Whitney test and Fisher's exact test. Optimal cutoff values to predict disease severity were determined by Youden's index. Results: In non-severe and severe groups, the median serum levels of IL-1β decreased on day 3 (1.72 ng/mL and 2.10 ng/mL, respectively), then increased on day 6 (2.05 ng/mL and 3.31 ng/mL, respectively). However, the median of IL-10 increased on day 3 (1.88 ng/mL and 2.30 ng/mL, respectively) and day 6 (2.02 ng/mL and 2.39 ng/mL, respectively). There was no significant association between the IL-1β:IL-10 ratio and COVID-19 severity at any time-point (p>0.05). The cutoff value of serum IL-10 between the two groups on days 0, 3, and 6 was 1.09 pg/mL (sensitivity: 66.6%; PPV: 71.4%), 2.11 pg/mL (sensitivity: 67.7%; PPV: 50.0%), and 2.08 pg/mL (sensitivity: 78.6%; PPV: 70.9%), respectively. Conclusion: The IL-1β:IL-10 ratio was not correlated to COVID-19 severity. However, owing to its high sensitivity, IL-10 may be a potential biomarker for disease severity in severe COVID-19.
Background Bilateral primary spontaneous pneumothorax (PSP) is a rare case of lung disease. Case ... more Background Bilateral primary spontaneous pneumothorax (PSP) is a rare case of lung disease. Case presentation A 20-year-old man with a complaint of shortness of breath is suspected of having PSP and tuberculosis. The patient underwent water seal drainage installation in both lung cavities, but showed no improvement. Multiple blebs were found after a few days. A wedge resection with VATS became an option. The patient had improved lung function after the procedure. Discussion The WSD installation showed lungs improvement. However, when trained for lung expansion, the lung condition became bad. After wedge resection with the help of VATS on multiple blebs, the lung had a significant improvement. Conclusion Wedge resection could be considered in PSP patients with multiple blebs.
Background. In Indonesia, highly pathogenic avian influenza A(H5N1) virus has become endemic in p... more Background. In Indonesia, highly pathogenic avian influenza A(H5N1) virus has become endemic in poultry and has caused sporadic deadly infections in human. Since 2012, we have conducted fixed-point surveillance of avian influenza viruses at a live-poultry market in East Java, Indonesia. In this study, we examined the seroprevalence of avian influenza A(H5N1) virus infection among market workers. Methods. Sera were collected from 101 workers in early 2014 and examined for antibody activity against avian A(H5N1) Eurasian lineage virus by a hemagglutination-inhibition (HI) assay. Results. By the HI assay, 84% of the sera tested positive for antibody activity against the avian virus. Further analysis revealed that the average HI titer in 2014 was 2.9-fold higher than in 2012 and that seroconversion occurred in 44% of paired sera (11 of 25) between 2012 and 2014. A medical history survey was performed in 2016; responses to questionnaires indicated that none of workers had had severe acut...
Background Pleuropulmonary amoebiasis caused by complications of amoebic liver abscess (ALA) is r... more Background Pleuropulmonary amoebiasis caused by complications of amoebic liver abscess (ALA) is rare. Case presentation A 23 years old male, presented with shortness of breath, cough with yellowish phlegm, right chest pain, fever, bulging stomach, yellow eyes, and swelling of both legs. Abdominal ultrasound and CT scan thorax and abdomen revealed right fluidopneumothorax and liver abscess. Serological testing leads to Entamoeba histolytica infection, which was treated with metronidazole but no significant improvement on empyema and abscess liver size. Surgery was performed after percutaneous aspiration drainage failed to evacuate the abscess. HE and PAS staining from surgical tissue showed Entamoeba hystolitica infection. Discussion Serological testing and radiological examination will be more useful in the early detection of cases of Entamoeba hystolitica infection. Surgery may be considered when purulent drainage does not show improvement in the patient's condition. Conclusion...
Annals of Medicine and Surgery
Indonesian Journal of Tropical and Infectious Disease, 2010
Indonesia is a greatest burden country of H5N1 avian influenza (AI) virus infection in the world,... more Indonesia is a greatest burden country of H5N1 avian influenza (AI) virus infection in the world, since first outbreak in Central Java 2005 until August 2010 there was 168 confirmed cases and 138 dead cases. The incidence increasing rapidly in widespread area endemic in Java, Sumatera, Bali and Sulawesi, and sporadic outbreaks in other areas. The World Health Organization stated that AI still became a treat in the next pandemic. H5N1 AI virus infection spreads in almost all provinces, but its endemic in Jakarta, Tangerang and Banten and in other area such Surabaya, Bali were sporadic outbreaks. There are 27 confirmed H5N1 AI infection cases in Jakarta from 296 suspected cases, while in Surabaya only 5 confirmed H5N1 AI infection cases from 12 suspected cases. The age of patient mean with H5N1 AI infection was 16.9 ± 11.6 yo in Jakarta and 24 ± 8.51 yo in Surabaya. There was no difference between male and female. Mortality rate was 77.7% in Jakarta and 60% in Surabaya. A large number...
Anaesthesia, Pain & Intensive Care
Indonesia has been fighting the COVID-19 pandemic since the beginning of March 2020, and it doesn... more Indonesia has been fighting the COVID-19 pandemic since the beginning of March 2020, and it doesn’t look that the situation is getting better any soon. Besides the country’s current strategies to minimize the rising mortality rate, a novel therapeutic intervention is required. After a thorough search in several databases, we found stem cells to be a likely candidate. Regardless of the general use of stem cells, studies showed positive results regarding the efficacy of using these in lung injuries. Especially, mesenchymal stem cells (MSCs) are known for their easy accessibility and their diverse mechanisms of action, including MSCs immunomodulatory antiviral effect, and its ability to improve lung function. Moreover, some researches perceived these components to be applicable in COVID-19 patients with end stage acute respiratory distress syndrome (ARDS). While randomized clinical trials are still in progress, many case reports show MSCs to be an advantageous alternative to suppress t...
Annals of Medicine and Surgery
BMC Infectious Diseases
Background The highly pathogenic avian influenza A/H5N1 virus is one of the causative agents of a... more Background The highly pathogenic avian influenza A/H5N1 virus is one of the causative agents of acute lung injury (ALI) with high mortality rate. Studies on therapeutic administration of bone marrow-derived mesenchymal stem cells (MSCs) in ALI caused by the viral infection have been limited in number and have shown conflicting results. The aim of the present investigation is to evaluate the therapeutic potential of MSC administration in A/H5N1-caused ALI, using a mouse model. Methods MSCs were prepared from the bone marrow of 9 to 12 week-old BALB/c mice. An H5N1 virus of A/turkey/East Java/Av154/2013 was intranasally inoculated into BALB/c mice. On days 2, 4, and 6 after virus inoculation, MSCs were intravenously administered into the mice. To evaluate effects of the treatment, we examined for lung alveolar protein as an indicator for lung injury, PaO2/FiO2 ratio for lung functioning, and lung histopathology. Expressions of NF-κB, RAGE (transmembrane receptor for damage associated ...
Jurnal Respirasi
Asthma is a heterogeneous chronic inflammatory disease in the respiratory tract that leads to rec... more Asthma is a heterogeneous chronic inflammatory disease in the respiratory tract that leads to recurrent episodic symptoms. Study about the mechanism of pathophysiology and immunology which stimulates chronic inflammation in asthma has been greatly developed. The understanding of inflammation mediator that is predominant on various asthma phenotypes could be useful for development of diagnosis and targeted therapy. Standard asthma therapy administered through the airway has limited effects only in the airway. The knowledge of molecular pathophysiology and immunology of this disease developed interest of the drugs that perform proximally from inflammation process in the airway, in this case is biological therapy. Several biological therapies have been investigated for its efficacy on human, including Anti IgE (Omalizumab), Anti Interleukin-5 (Mepolizumab, Reslizumab, Benralizumab), Anti Interleukin-4/Interleukin-13 (Dupilumab), and Anti Interleukin-17 (Secukinumab and Brodalumab).
Jurnal Respirasi
Pneumocystis Pneumonia (PCP) is a potentially life-threatening infection that can occur in indivi... more Pneumocystis Pneumonia (PCP) is a potentially life-threatening infection that can occur in individuals who are immunocompromised. In PCP steroid use is still recommended especially in patients with moderate and severe severity. Corticosteroids are given along with anti-pneumocystis therapy and are known to reduce the incidence of mortality and respiratory failure associated with PCP. Innate immunity and adaptive immunity are symbiotic relationships to provide optimal defense for the lungs and other organs and tissues from infection PCP. The corticosteroid mechanism in PCP is based on an anti-inflammatory mechanism especially its role in inhibiting neutrophils. Many clinicians believe the administration of anti-pneumocystis causes the acceleration of inflammation. Because the inflammatory process increases when anti-pneumocystis therapy is started, corticosteroid therapy is useful before inflammation occurs which causes extensive damage to the lungs.
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Papers by Resti Yudhawati