This research was carried out to isolateHistoplasma capsulatum from respiratory samples of horses... more This research was carried out to isolateHistoplasma capsulatum from respiratory samples of horses in Iraq using genomic detection.A total numbers of one hundred (100) nasal swabs andone hundred (100) blood samples were collected from equestrian clubs in Baghdad, DhiQar, Addiwaniya and Najaf provinces.All samples cultured on sabouraud dextrose agar (SDA) and brain heart infusion agar (BHIA) with chloramphenicol and cycloheximide and incubatedat (25°C and 37°C) for (4 weeks). The samples wereexamined macroscopically depending on shape, texture and color of the fungalgrowth and microscopically by preparing a mount smear with lactophenol cotton blue and examined under (40X). The diagnosisconfirmed by molecular techniques for the detection ofHistoplasma capsulatum by using conventional PCR.So, the isolation ofHistoplasma capsulatumwas(4%)in both conventional examination and molecular detection innasal swabs,but in case of blood samples,the Histoplasma capsulatum isolatedby conventional methods was 3%,whereas the molecular detection appeared as 2%.
This research was carried out to isolateHistoplasma capsulatum from respiratory samples of horses... more This research was carried out to isolateHistoplasma capsulatum from respiratory samples of horses in Iraq using genomic detection.A total numbers of one hundred (100) nasal swabs andone hundred (100) blood samples were collected from equestrian clubs in Baghdad, DhiQar, Addiwaniya and Najaf provinces.All samples cultured on sabouraud dextrose agar (SDA) and brain heart infusion agar (BHIA) with chloramphenicol and cycloheximide and incubatedat (25°C and 37°C) for (4 weeks). The samples wereexamined macroscopically depending on shape, texture and color of the fungalgrowth and microscopically by preparing a mount smear with lactophenol cotton blue and examined under (40X). The diagnosisconfirmed by molecular techniques for the detection ofHistoplasma capsulatum by using conventional PCR.So, the isolation ofHistoplasma capsulatumwas(4%)in both conventional examination and molecular detection innasal swabs,but in case of blood samples,the Histoplasma capsulatum isolatedby conventional methods was 3%,whereas the molecular detection appeared as 2%.
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