Papers by Raj nandan Tiwari
2010 3rd International Conference on Emerging Trends in Engineering and Technology, 2010
... & Engg. Mizan Tepi University ETHIOPIA [email protected] Anil Agrawal Dept. of Comp... more ... & Engg. Mizan Tepi University ETHIOPIA [email protected] Anil Agrawal Dept. of Comp Sc. ... Here we use the concept of content-based recommendation to establish the dimension profile. Each dimension profile incorporates the attribute properties and its weighted value. ...
Proceedings of the International Conference & Workshop on Emerging Trends in Technology - ICWET '11, 2011
Abstract The glitch of providing recommendations to the visitors of a web site has received a sig... more Abstract The glitch of providing recommendations to the visitors of a web site has received a significant amount of attention in recent years. To address this requirement, web sites provide personalized recommendations to the end users. When a personalization system ...
Public reporting burden for the collection of information is estimated to average 1 hour per resp... more Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number.
Cancer Research, 2014
Cancer growth and progression is influenced by tumor cells, stromal cells, as well as various sec... more Cancer growth and progression is influenced by tumor cells, stromal cells, as well as various secreted molecules found within the tumor microenvironment (TME). These secreted molecules participate in various crosstalk events that contribute to cancer development and progression. One such secreted molecule found within the tumor microenvironment is the proinflammatory cytokine interleukin-8 (IL-8). IL-8 influences cancer development by binding to its G-protein coupled receptors CXCR1 and CXCR2 in turn leading to activation of various signal transduction pathways that promotes cell proliferation, survival, angiogenesis, and invasion. We hypothesized that infiltrating macrophages and tumor cells participate in cellular crosstalk through the release of inflammatory cytokines which function to modulate the breast cancer phenotype. Using a cell culture model, conditioned media (CM) generated from activated THP-1 monocytes as well as recombinant IL-8 (rIL-8) were individually tested on the...
Cancer Research, 2014
The incidence of thyroid cancer in the United States has significantly increased over the past de... more The incidence of thyroid cancer in the United States has significantly increased over the past decade, with over 60,220 cases predicted for 2013. While premenopausal women have an approximately four fold higher incidence of papillary thyroid cancer (PTC) than men, once diagnosed, PTC in men exhibits increased aggressiveness and results in poorer clinical outcomes. These paradoxical observations led us to investigate the role that androgens and/or androgen receptors play in PTC. Analysis of AR expression in 24 PTC patient tissue samples indicated a 5.97 fold reduction in AR expression compared to matched, normal tissue (P<0.0001). To more fully investigate the function of androgen/AR in thyroid cells in a more controlled experimental system, the AR cDNA (pSG5-AR) was subcloned into the pcDNA3.1+ expression vector and stably transfected into 8505C anaplastic/PTC cells. Addition of DHT to the 8505C-transfected clone, 84E7, resulted in AR translocation into the nucleus and a 48% redu...
Anticancer research
The etiology of human breast cancer is poorly understood and no specific marker of transformation... more The etiology of human breast cancer is poorly understood and no specific marker of transformation has been identified. Amplification of HER-2/neu, as reported in a comprehensive study by Slamon et al, was found to be the most powerful predictor of disease-free and overall survival after the status of the axillary lymph nodes. Our study examines the HER-2/neu oncogene in 61 primary human breast cancers at both the DNA level (by Southern blotting) and the protein level (by immunohistochemical methods). Of the 61 tumors analyzed in our study, 17 (28%) had amplification of HER-2/neu. There was no significant correlation of HER-2/neu amplification with age, tumor diameter or hormone receptor status; however, amplification and overexpression of HER-2/neu was significantly correlated with the status of the axillary lymph nodes (P = 0.02). Of 16 patients with amplification of HER-2/neu, 14 (88%) had positive regional nodes. One of the two node negative cases with amplified HER-2/neu had bon...
In vivo (Athens, Greece)
The role of body fat as a risk factor for breast cancer has been well established. A decrease in ... more The role of body fat as a risk factor for breast cancer has been well established. A decrease in the urinary 2/16α-hydroxyestrone ratio has also been shown to be a risk marker for breast cancer. These two observations are connected by the fact that obese women have decreased levels of 2-hydroxyestrone. To test the hypothesis that fat depots secrete factors that inhibit 2-hydroxylation, the effect of substances released into the media from adipocytes incubated in Krebs-Ringer buffer, on estrogen metabolism by MCF-7 cells in minimum essential medium eagle (MEM) plus adipocyte-conditioned media (ACM) was studied. The 1:1 ACM-MEM culture system resulted in a substantial and highly significant decrease in 2-hydroxylation of estradiol. This inhibition was partially reversed by the addition of indole-3-carbinol, a potent inducer of 2-hydroxylation of estradiol. Centrifugal sizing showed that the active 2-hydroxylation inhibitor in the medium had a molecular weight of about 30 kDa. These re...
International Journal of Computer Applications, 2010
Integration of data sources to build a Data warehouse (DW), refers to the task of developing a co... more Integration of data sources to build a Data warehouse (DW), refers to the task of developing a common schema as well as data transformation solutions for a number of data sources with related content. The large number and size of modern data sources make the integration process cumbersome. In such cases dimensionality of the data is reduced prior to populating the DWs. Attribute subset selection on the basis of relevance analysis is one way to reduce the dimensionality. Relevance analysis of attribute is done by means of correlation analysis, which detects the attributes (redundant) that do not have significant contribution in the characteristics of whole data of concern. After which the redundant attribute or attribute strongly correlated to some other attribute is disqualified to be the part of DW. Automated tools based on the existing methods for attribute subset selection may not yield optimal set of attributes, which may degrade the performance of DW. Various researchers have used GA, as an optimization tool but most of them use GA to search the optimal technique amongst the available techniques for attribute selection. This paper formulates and validates a method for selecting optimal attribute subset based on correlation using Genetic algorithm (GA), where GA is used as optimal search tool for selecting subset of attributes. .
International Journal of Computer Applications, 2011
The Internet has become a cosmic information source in recent years and can be considered as the ... more The Internet has become a cosmic information source in recent years and can be considered as the world's largest digital library. To aid ordinary users in finding desired data in this library, numerous search engines have been created. Each search engine has a corresponding database that defines the set of documents that can be searched by the search engine. Typically, an index for all documents in the database is created and stored in the search engine. Text data in the Internet can be partitioned into numerous databases naturally. Proficient retrieval of desired data can be realized if we can accurately envisage the usefulness of each database, because with such information, we only need to retrieve potentially useful documents from useful databases. For a given query "q" the usefulness of a text database is defined to be the no. of documents in the database that are sufficiently relevant to the query "q". In this paper, we propose innovative approaches for database selection and documents selection.
Oncology letters, 2011
Neo-vascularization is essential for tumor growth and metastasis and is presumably initiated by b... more Neo-vascularization is essential for tumor growth and metastasis and is presumably initiated by bone marrow-derived endothelial progenitor cells (BM-EPCs). These cells predominantly reside in the bone marrow and are recruited at sites of inflammation, tissue damage and tumors. The tissue-specific factors responsible for recruitment of BM-EPCs and neo-vascularization are the subject of intense investigation. Using bone marrow cells from Tek/green fluorescent protein (GFP) transgenic mice, we analyzed the effect of estrogen on the mobilization of BM-EPCs to orthotopically implanted cancer cells in estrogen- and non-estrogen-supplemented ovariectomized mice. The donor marrow cells were unique as they were fluorescently tagged, allowing for the tracking of their migration to the tumor tissues. Results showed that GFP + BM-EPCs were incorporated within the tumor vasculature in comparison to the sham injections. Notably, estrogen supplementation enhanced the mobilization of BM-EPCs to the...
International Journal of Applied Evolutionary Computation, 2012
Data Warehouses (DWs) are aimed to empower the knowledge workers with information and knowledge w... more Data Warehouses (DWs) are aimed to empower the knowledge workers with information and knowledge which helps them in decision making. Technically, the DW is a large reservoir of integrated data that does not provide the intelligence or the knowledge demanded by users. The burden of data analysis and extraction of information and knowledge from integrated data still lies upon the analyst’s shoulder. The overhead of analysts can be taken off by architecting a new generation data warehouses systems those shall be capable of capturing, organizing and representing knowledge along with the data and information in it. This new generation DW may be called as Knowledge Warehouse (KW) shall exhibit decision making capabilities themselves and can also supplement the Decision Support Systems (DSS) in making decisions quickly and effortlessly. This paper proposes and simulates a fuzzy-rule based adaptive knowledge warehouse with capabilities to learn and represent implicit knowledge by means of a...
International Journal of Computer Applications, 2011
Use of the Internet and the World Wide Web has improved swiftly over the past few years. Personal... more Use of the Internet and the World Wide Web has improved swiftly over the past few years. Personals deploying Web servers crave to appreciate how their servers are being used by Internet users, how those patterns of use are changing over time, and what steps they should take to ensure adequate server response to the incoming requests today and in the future. This requires an evaluation of the requests offered to the Web server and the characteristics of the server's response to those requests over a suitably long time interval. In this paper we present the results of a study of Web server system of http://www.lawetalnews.com for a six month period. In this duration the traffic to the website raised drastically in terms of incoming requests and outgoing bytes. We study the request and response types, and portray the traffic distribution on the basis of request size, response time, as well as some other factors. We wind up with system performance recommendations and identify future directions for our Web research.
Medicinal Plants - International Journal of Phytomedicines and Related Industries, 2013
Oral cavity of human being is full of micro-flora, viz., bacteria and fungi. Oral swab was collec... more Oral cavity of human being is full of micro-flora, viz., bacteria and fungi. Oral swab was collected from 100 healthy persons and cultured on blood agar media. Effect of aqueous extracts of Azadirachta indica, Mentha arvensis var. Kosi, Achyranthes aspera and Catharanthus roseus was tested for their efficacy in preventing oral bacterial growth. Popular commercially available tooth paste was used as control. Most effective results were obtained from Azadirachta indica and Achyranthes aspera in comparison to the control. The extracts of other two medicinal plants also showed good results.
Proceedings of the Third Annual ACM Bangalore Conference, 2010
Permission to make digital or hard copies of all or part of this work for personal or classroom u... more Permission to make digital or hard copies of all or part of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. To copy ...
International Journal of Computer Applications, 2010
Science of The Total Environment, 1997
As interest in the properties of xenoestrogenic compounds has grown, different in vitro cell cult... more As interest in the properties of xenoestrogenic compounds has grown, different in vitro cell culture systems have been proposed as models, against which to gauge relative estrogenic impact. Previous research indicated that some organochlorine-based pesticides elevated the production of 16~hydroxyestrone relative to 2-hydroxyestrone in ER+ MCF-7 breast cancer cells while phytochemicals like indole-3-carbinol reduced this ratio. That this ratio may be a biological marker of the risk of breast cancer has recently been demonstrated. In this study we have carried out the same paradigm in two ER-cell lines to examine the effect of receptor status. To determine whether the impact of chlorinated pesticides can be modulated by phytochemicals, the ability of indole-3-carbinol or brassinin to reverse the changes in metabolism was examined. Non-persisting phosphorus-based pesticides were also studied and shown not to have an effect on estrogen metabolism. The implications of these findings are examined. 0 1997 Elsevier Science B.V.
JNCI Journal of the National Cancer Institute, 1994
Indole-3-carbinol (I3C), a compound found in cabbage, broccoli, and brussels sprouts, inhibits th... more Indole-3-carbinol (I3C), a compound found in cabbage, broccoli, and brussels sprouts, inhibits the growth of mammary tumors when fed to certain strains of mice. The chemopreventive and antitumor effects of I3C depend on the species and tissue type. The mechanism of action and specific human cell types that respond to I3C are not known. Our purpose was to study the mechanism of action of I3C in estrogen-responsive (MCF-7) and estrogen-nonresponsive (MDA-MB-231) human breast cancer cell lines. Estrogen responsiveness was determined by the ability of estradiol to stimulate the growth of breast cancer cells deprived of estrogen. The effect of I3C was measured on cell growth, estradiol metabolism, and level of cytochrome P-4501A1. Growth was measured by cell counts and soft-agar assay, estrogen metabolism was examined by a radiometric assay, and the level of cytochrome P-4501A1 was measured by Western blots with a polyclonal antibody. I3C inhibits the growth of estrogen-responsive cell line MCF-7 but has little effect on estrogen-nonresponsive cell line MDA-MB-231. Specific C-2 hydroxylation of estrogen and induction of cytochrome P-4501A1 was enhanced by I3C in the MCF-7 but not in the MDA-MB-231 cells. I3C has specific antigrowth effects in human breast cancer cells. The inhibitory effects of I3C may involve selective induction of estradiol metabolism and the related cytochrome P-450 system that may be limited to estrogen-sensitive cells.
Journal of Herbs, Spices & Medicinal Plants, 2008
... lines of opium poppy and three male testers were selected on the basis of seed and straw yiel... more ... lines of opium poppy and three male testers were selected on the basis of seed and straw yield from diverse poppy cultivating areas ... Seed protein content was determined using the method ofJackson (6). Morphine content of the straw was determined by applying the method of ...
Journal of Health, Population and Nutrition, 2013
The Government of India initiated a cash incentive scheme-Janani Suraksha Yojana (JSY)-to promote... more The Government of India initiated a cash incentive scheme-Janani Suraksha Yojana (JSY)-to promote institutional deliveries with an aim to reduce maternal mortality ratio (MMR). An observational study was conducted in a tertiary-care hospital of Madhya Pradesh, India, before and after implementation of JSY, with a sample of women presenting for institutional delivery. The objectives of this study were to: (i) determine the total number of institutional deliveries before and after implementation of JSY, (ii) determine the MMR, and (iii) compare factors associated with maternal mortality and morbidity. The data were analyzed for two years before implementation of JSY (2003-2005) and compared with two years following implementation of JSY (2005-2007). Overall, institutional deliveries increased by 42.6% after implementation, including those among rural, illiterate and primary-literate persons of lower socioeconomic strata. The main causes of maternal mortality were eclampsia, pre-eclampsia and severe anaemia both before and after implementation of JSY. Anaemia was the most common morbidity factor observed in this study. Among those who had institutional deliveries, there were significant increases in cases of eclampsia, pre-eclampsia, polyhydramnios, oligohydramnios, antepartum haemorrhage (APH), postpartum haemorrhage (PPH), and malaria after implementation of JSY. The scheme appeared to increase institutional delivery by at-risk mothers, which has the potential to reduce maternal morbidity and mortality, improve child survival, and ensure equity in maternal healthcare in India. The lessons from this study and other available sources should be utilized to improve the performance and implementation of JSY scheme in India.
Cancer Research, 2011
Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL Papillary thyroid carcin... more Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL Papillary thyroid carcinoma (PTC) is the most common endocrine and thyroid malignancy. The urokinase plasminogen activator receptor (uPAR) plays an important role in cancer pathogenesis, including breakdown of the extracellular matrix, invasion, and metastasis. Additionally, there is increasing evidence that uPAR also promotes tumorigenesis via the modulation of multiple signaling pathways. BRAFV600E, the most common initial genetic mutation in PTC, leads to ERK1/2 hyperphosphorylation, which has been shown in numerous cancers to induce uPAR. Treatment of the BRAFV600E-positive PTC cell line, BCPAP, with the MEK/ERK inhibitor U0126 reduced uPAR RNA levels by 90%. siRNA-mediated down-regulation of uPAR in BCPAP cells resulted in drastically reduced proliferation rates and decreased clonigenic survival, as well as demonstrated senescence-associated nuclear morphology and induction of β-galactosidase activity. uPAR-knockdown BCPAP cells also displayed greatly reduced migration and invasion rates, as well as a complete loss of the cells’ plasminogen-enhanced invasiveness. Additionally, uPAR down-regulation lead to greatly decreased activity in the focal adhesion kinase (FAK)/phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway, as well as a modest reduction in ERK1/2 signaling (which remained constitutively active secondary to BRAFV600E activation), which suggests the existence of a uPAR signaling feedback loop capable of affecting the various phenotypic changes exhibited by uPAR-knockdown cells. Taken together, these data provide new evidence of a novel role for uPAR induction as a central component in PTC pathogenesis, and highlight the potential of uPAR as a therapeutic target. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 1406. doi:10.1158/1538-7445.AM2011-1406
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Papers by Raj nandan Tiwari