Papers by Peyman Ghoraishizadeh
International Journal of Applied Sciences and Biotechnology, Dec 25, 2014
InTech eBooks, Nov 29, 2017
Mesenchymal Stem Cells - Isolation, Characterization and Applications
Background: Myelodysplastic syndrome is a mixed clonal disorder of bone marrow progenitor cells. ... more Background: Myelodysplastic syndrome is a mixed clonal disorder of bone marrow progenitor cells. Understanding the pattern of the different lineage-specific, immature, and mature markers in myelodysplastic syndrome will help in setting-up the frame of reference to diagnose. Patients and Methods: We compared 60 bone marrow samples from 30 newly-diagnosed patients with myelodysplastic syndrome and 30 patients with idiopathic thrombocytopenic purpura as the control to perform a quantitative analysis of the antigen expression patterns in granulocytic, monocytic, erythroid and lymphoid lineages and myeloid precursors. Results: Quantitative analysis of CD markers, showed that the mean percentages of CD33, CD13, CD11b, HLA-DR, CD10 and CD34 positive granulocytes were 91%, 84.98%, 77.20%, 14.59%, 40.34% and 34.25%, respectively in myelodysplastic syndrome and 96.89%, 91.57%, 81.47%, 10.56%, 58.30% and 32.37%, respectively in idiopathic thrombocytopenic purpura. Flow cytometric analysis of e...
Journal of Medicine and Biomedical Sciences, 2014
ABSTRACT antigen expression pattern in refractory anemia, refractory anemia with ringed siderobla... more ABSTRACT antigen expression pattern in refractory anemia, refractory anemia with ringed sideroblasts and refractory anemia with excess blast cases using flow cytometry. Journal of Medicine and Biomedical Sciences 2013; 4(2), 49-56. DOI: 10.7813/jmbs.2013/4-2/8 ABSTRACT Myelodysplastic Syndromes (MDSs) are a slow progressing group of disease that causes peripheral cytopaenia of all cell lineages, with high capabilities to transform into acute myeloid leaukaemia (AML). Establishing a frame of reference for antigen expression pattern in different MDS subtypes, may help to classify the patients for choosing more appropriate therapeutic approaches. In this study, thirty bone marrow (BM) samples from newly-diagnosed MDS patients were analyzed by 4-colour FACS Canto flow cytometer. In this research, the quantitative analysis of antigen expression patterns of granulocytic, monocytic, erythroid and lymphoid lineages also myeloid precursors were performed. The one-way ANOVA was used to test the differences between mean percentages of antigens in MDS subtypes. In this study, we showed the mean percentages of CD71/CD235a/CD45/CD117, HLA-DR/CD13/CD45/CD11b, CD14/CD33/CD45/CD34, and CD19/ CD20/CD45/CD10 antibody combinations on erythroid, granulocytic, monocytic, and lymphoid lineages, respectively, in different MDS subtypes. The most important finding of this study was the significant difference of CD71 mean percentage in erythroid cells between MDS subtypes. Erythroid lineage in Refractory Anemia with Excess Blast (RAEB) cases was found by lower mean percentage of CD71 (54.74%), as compared to Refractory Anemia (RA) (74.65%) and Refractory Anemia with Ringed Sideroblasts (RARS) cases (74.60%). In this study, the difference of CD71 + erythroid cells was statistically significant only between RARS and RAEB subtypes (p=0.011). Our study, showed the expression range of different CD markers on different lineages of MDS subtypes. These findings can improve understanding the prognosis of various subtypes, and explaining the laboratory and clinical results.
Advances in Regenerative Medicine, 2014
Regenerative medicine is an alternative solution for organ transplantation. Stem cells and nanosc... more Regenerative medicine is an alternative solution for organ transplantation. Stem cells and nanoscaffolds are two essential components in regenerative medicine. Mesenchymal stem cells (MSCs) are considered as primary adult stem cells with high proliferation capacity, wide differentiation potential, and immunosuppression properties which make them unique for regenerative medicine and cell therapy. Scaffolds are engineered nanofibers that provide suitable microenvironment for cell signalling which has a great influence on cell proliferation, differentiation, and biology. Recently, application of scaffolds and MSCs is being utilized in obtaining more homogenous population of MSCs with higher cell proliferation rate and greater differentiation potential, which are crucial factors in regenerative medicine. In this review, the definition, biology, source, characterization, and isolation of MSCs and current report of application of nanofibers in regenerative medicine in different lesions ar...
Russian Open Medical Journal, 2014
International Journal of Applied Sciences and Biotechnology, 2014
Pseudomonas aeruginosa (PA) as an opportunistic pathogen infects the pulmonary tract, bladder, cy... more Pseudomonas aeruginosa (PA) as an opportunistic pathogen infects the pulmonary tract, bladder, cystic fibrosis patients and burn victims. PA infections treatment is challenging because of its ability to rapidly develop resistance to multiple classes of antibiotics. Lectin is protein that isexpressed in cell of PA and cause of infection by attaching to the host cells. Lectin A gene coding lectin protein so we cloned and expressedthis gene then purified of related protein, that can be used in preparation of vaccine to treat PA infections.DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11559 Int J Appl Sci Biotechnol, Vol. 2(4): 529-536
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Papers by Peyman Ghoraishizadeh