Biotechnology and Applied Biochemistry, Apr 19, 2021
In this work, a nickel ferrite (NiFe2O4)/nitrogen‐doped‐graphene (NG)/cellulose composite (NiFe2O... more In this work, a nickel ferrite (NiFe2O4)/nitrogen‐doped‐graphene (NG)/cellulose composite (NiFe2O4/NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated. The synthesized NiFe2O4/NG/cellulose composite was characterized by performing morphological and structural analyses. The results showed the successful formation of NiFe2O4‐nanoparticles with a spherical shape and a size ranging from 15 to 200 nm. Energy‐dispersive X‐ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture. The X‐ray diffraction pattern showed the face‐centered‐cubic nature of the particles. In addition, antibacterial activity against Escherichia coli (Gram‐negative bacteria) and Bacillus subtilis (Gram‐positive bacteria) was evaluated with different concentrations of NiFe2O4/NG/cellulose composite (0–50 μg/mL). Inhibitory activity increased with increasing concentrations of NiFe2O4/NG/cellulose. The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures. Overall, the chemically synthesized NiFe2O4/NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.
International Journal of Biological Macromolecules, May 1, 2013
A total of 18 protease producing bacterial strains were isolated from detergent effluent in South... more A total of 18 protease producing bacterial strains were isolated from detergent effluent in South Korea using skim milk agar medium. A strain (BK-P21A) was selected and identified as Bacillus koreensis based on morphological, biochemical and molecular characterizations (16S rRNA gene sequence analysis). Optimized culture conditions for the production of protease were pH 8.5, 30 °C, sucrose (2%) and yeast extract (0.2%) during 36 h of incubation. Furthermore, the protease was partially purified by ammonium sulphate precipitation (80%) and again by Superdex 200 10/300 GL and Superdex 75 10/300 GL column chromatography, which resulted in 5.0 fold purification and a yield of 23%. The molecular mass of the protease was estimated to be 48 kDa by SDS-PAGE. The purified enzyme was further characterized and found to be most active at pH 9.0 and 60 °C. The activity of the purified protease was enhanced by CaCl₂ and CoCl₂, but inhibited by PMSF, which indicated it was a serine type protease. Moreover, the protease was moderately stable in surfactants and 81% stable in H₂O₂. Finally, the enzyme was more active and stable (94-126.5%) in various hydrophilic organic solvents. Considering the stability of protease towards the alkaline pH, high temperature and organic solvents (50%), the enzyme from B. koreensis can be used as an alternative biocatalyst for several industrial applications mainly for peptide synthesis in nonaqueous solvents.
The fast growing trees were had the acceptable growth, chemical composition and morphological pro... more The fast growing trees were had the acceptable growth, chemical composition and morphological properties that make it suitable for ethanol recovery. The growth attained average level from 1.57m to 3.73m at the end of ninth month. The chemical composition such as higher proportion of holocellulose and lower content of lignin of the fast growing trees expected the more ethanol recovery with minimum recalcitrant. Due to long fiber length, wider fiber width, and wide lumen diameter with thin cell wall thickness of the fast growing trees were preferable for ethanol conversion.
The short rotation trees had longer fiber length, higher slenderness ratio, flexibility coefficie... more The short rotation trees had longer fiber length, higher slenderness ratio, flexibility coefficient and lower Runkel ratio and rigidity coefficient with higher proportion of holocellulose content acceptable lignin, ash and pentasons. These preporties directly related to the high burst strength, tensile strength, tearing strength and folding endurance of the paper. The greatest proportion of fiber length in M. dubia (1022.36μm) and the lowest in Eucalyptus clones (538.80μm). The higher content of holocellulose in A. fraxinifolis (74.2%), P.deltoides (74.15%) and M.dubia (74%) species were recorded and significantly low value in L. leucocephala (70.81%). So, the short rotation trees were more preferable for paper production based on the morphological and chemical properties.
The relevance of maternal macronutrient deficiency on developmental origin of health and adult di... more The relevance of maternal macronutrient deficiency on developmental origin of health and adult disorders has been well studied but not that of micronutrients. We hypothesized that chronic maternal dietary mineral (copper or zinc) or vitamin E restriction modulates the expression of placental 11 beta hydroxysteroid dehydrogenase-2 (11 beta HSD-2) per se predisposing the offspring to insulin resistance (IR) and hypertension in later life. Female weaning Swiss albino mice received a control or a 50% of Vitamin-E or Zn or Cu restricted diet and mated with control males. Pups born to the dams on the restricted diet had significantly (P < 0.001) reduced body weight and crown rump length. These offsprings were weaned on to the restricted diet till postnatal day 180. Glucose intolerance in association with hyperinsulinemeia (IR), hyperlipidemeia and increased systolic blood pressure were recorded in all the offsprings of micronutrient restricted groups. Placental 11 beta HSD-2 expression was attenuated, while activities of glucocorticoid -insensitive enzymes were unchanged in all the restricted groups. Thus, the present study reiterates the importance of micronutrients during pregnancy because chronic maternal micronutrient deficiency may alter placental 11 beta HSD-2 expression and predispose the offspring to IR and hypertension in later life.
Journal of Industrial Microbiology & Biotechnology, Feb 1, 2014
We previously completed whole-genome sequencing of a rare actinomycete named Sebekia benihana, an... more We previously completed whole-genome sequencing of a rare actinomycete named Sebekia benihana, and identified the complete S. benihana cytochrome P450 complement (CYPome), including 21 cytochrome P450 hydroxylase (CYP), seven ferredoxin (FD), and four ferredoxin reductase (FDR) genes. Through targeted CY-Pome disruption, a total of 32 S. benihana CYPome mutants were obtained. Subsequently, a novel cyclosporine A region-specific hydroxylase was successfully determined to be encoded by a CYP-sb21 gene by screening the S. benihana CYPome mutants. Here, we report that S. benihana is also able to mediate vitamin D 3 (VD 3) hydroxylation. Among the 32 S. benihana CYPome mutants tested, only a single S. benihana CYP mutant, DCYP-sb3a, failed to show regio-specific hydroxylation of VD 3 to 25-hydroxyvitamin D 3 and 1a,25-dihydroxyvitamin D 3. Moreover, the VD 3 hydroxylation activity in the DCYP-sb3a mutant was restored by CYP-sb3a gene complementation. Since all S. benihana FD and FDR disruption mutants maintained VD 3 hydroxylation activity, we conclude that CYP-sb3a, a member of the bacterial CYP107 family, is the only essential component of the in vivo regiospecific VD 3 hydroxylation process in S. benihana. Expression of the CYP-sb3a gene exhibited VD 3 hydroxylation in the VD 3 non-hydroxylating Streptomyces coelicolor, implying that the regio-specific hydroxylation of VD 3 is carried out by a specific P450 hydroxylase in S. benihana. Keywords Sebekia benihana Á Vitamin D 3 Á Cytochrome P450 hydroxylation Á Rare actinomycetes J.-G. Ban and H.-B. Kim contributed equally to this paper. Special issue: Genome Mining for Natural Products Discovery.
The aim of this research is to study the morphological analysis of fabricated Interdigitated Elec... more The aim of this research is to study the morphological analysis of fabricated Interdigitated Electrode (IDE). This device electrode was physically characterized using 3D nano profiler, scanning electrode microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Atomic Force Microscope (AFM). Based on this analysis, IDE pattern was analyzed thoroughly based on the IDE pattern specifications with 5 μM finger gap and this research significantly will stand as a platform quantify the biomolecules in further analysis.
Biotechnology and Applied Biochemistry, Mar 1, 2013
The novel protease from Exiguobacterium profundum BK-P23 was partially purified by ammonium sulfa... more The novel protease from Exiguobacterium profundum BK-P23 was partially purified by ammonium sulfate precipitation and further purified Mono Q 5/50 and Superdex 200 10/300 column chromatography. The enzyme was purified 10.23-fold with a yield of 14%. The molecular weight was estimated to be 52 kDa by SDS-PAGE. The enzyme was most active at a pH of 8.0 and temperature of 40 • C and the enzyme was stable between a pH of 7 and 10 and up to a temperature of 50 • C. The enzyme activity was enhanced by CaCl 2 but was slightly inhibited by CoCl 2 , MgSO 4 , and AgNO 3. In addition, this enzyme was completely inhibited by phenylmethylsulfonyl fluoride, indicating that this enzyme was a serine protease. Furthermore, the alkaline protease was more stable in the presence of surfactants such as Triton X-100, which was followed by Tween 80 and SDS. Moreover, the enzyme was highly stable in the presence of 1% oxidizing agent (H 2 O 2). The enzyme also has significant stability (70%-80%) in a few organic solvents. Thus, the increased stability of the enzyme in the presence of oxidizing agent, surfactants, and organic solvents may find potential applications in the detergent industry and peptide synthesis in nonaqueous media.
Journal of The Taiwan Institute of Chemical Engineers, Apr 1, 2021
Abstract Diagnosing gastric cancer with a high accuracy helps in identifying at early stage and f... more Abstract Diagnosing gastric cancer with a high accuracy helps in identifying at early stage and for a better treatment. A transcription factor, SRY-box containing gene-17 (SOX-17) shows a crucial role in the gastric cancer progression is desired as biomarker on a high-performance sensing platform, dimicroelectrodes Junction (DMEJ), constructed by the hybrid of single-walled carbon nanotube (SWCN) and gold urchin (GU) surface to detect the specific SOX-17 target oligonucleotide. SWCN was modified on DMEJ and GU conjugated capture DNA was immobilized on SWCN through the electrostatic interaction of GU by (3-Aminopropyl)triethoxysilane. Morphological analyses by FESEM and FETEM revealed the attachment of GU on SWCN with a uniform fashion. SWCN-GU modified DMEJ has enhanced an ideal electric flow and reflecting the capture DNA attachment. Reached the limit of detection at 1 aM, calculated on a linear curve (atto- to femto-molar) with the regression coefficient of R2 = 0.9909[y = 1.1226x + 1.3727]. Further, specificity of SOX-17 was confirmed by interacting complementary, single- and five-mismatched sequences of the target. In addition, the target DNA solution mixed human serum and control sequences did not interfere with the specific duplex formation, indicating the non-biofouling on DMEJ surface. This SWCN-GU constructed DMEJ surface helps to identify lower expression of SOX-17 and diagnosing gastric cancer progression.
The present study aimed to characterize CoNPs that were synthesized using a simple, inexpensive, ... more The present study aimed to characterize CoNPs that were synthesized using a simple, inexpensive, and eco-friendly way from algal-extract of Elodea canadensis. Further, this study also assessed the antimicrobial potential of the synthesized CoNPs. The CoNPs formation was preliminarily proved through colour change from light-pink to brown within 15 min upon adding the algal-extract to the cobalt (II) nitrate solution. The maximum absorption peaks at 450 nm for the algae-encapsulated CoNPs were obtained using UV-Vis spectrum. FTIR result proved that CoNPs were capped with active components from E. canadensis as distinct peaks were observed at 3288.4 and 1641.8 cm−1 in the infrared spectra of the algal-extract and bio-reduced CoNPs. A FE-SEM revealed that CoNPs were agglomerated and had smooth surfaces. In addition, FE-TEM indicated that the synthesized CoNPs had assorted sizes and shapes. Meanwhile, the XRD displayed several broad peaks at 2θ = 52.37°, 54.21°, and 56.09° with mean size of 14.022 nm that affirmed the crystalline nature of algal synthesized CoNPs. The antimicrobial potential of the biosynthesized CoNPs was assessed using the disc-diffusion test.
Oncogene-mutated breakpoint cluster region protein gene (BCR/ABL) has been found in chronic myelo... more Oncogene-mutated breakpoint cluster region protein gene (BCR/ABL) has been found in chronic myelogenous leukemia (CML) patients. In particular, b3a2 is a common mutation type with CML, tested in this research as deoxyribonucleic acid sensor to identify the specific b3a2 sequence by a suitable capture probe. The aminated capture probe was attached on interdigitated electrode surface through the chemical linker, glycidyloxypropyltrimethoxysilane. Covalently linked reduced graphene oxide (rGO) with target sequence was identified by the capture probe. The prepared rGO from vacuum-assisted low-temperature exfoliated graphite was characterized by field emission scanning electron microscope, field emission transmission electron microscope, energy-dispersive X-ray spectroscopy and fourier-transform infrared spectroscopy. The limit of detection with duplex formation was attained between 1 and 10 aM, and the saturation point was noticed from 10 fM. The sensitivity was calculated to be 1 aM. Further, control experiments with single-, triple-and complete mismatch-sequences instead of target complementary failed to yield the changes in current signal indicating the selective identification of targeted b3a2 sequence. This method of detection helps in identifying the minimal leukemia cells residual with CML patient.
� Using Chinese joss paper silica-aluminium nanocomposite produced by alkalinization. � Character... more � Using Chinese joss paper silica-aluminium nanocomposite produced by alkalinization. � Characterized the silica and aluminium nanocomposite and purity was checked. � Nanocrystal synthesized �15 nm size & spherical. Ratio is 13.24 to 7.96 � Band gap of nanocomposite is 3.84 eV. � Optically measured agglomeration is 36.74 μm, 3D-nanoprofiler height is 234.37 μm.
Different nanostructures were engineered with the nanoscale dimension lesser than 100 nm. These n... more Different nanostructures were engineered with the nanoscale dimension lesser than 100 nm. These nanostructures include silver, cellulose nanoparticles and single-walled carbon nanotube (SWCNT). Biological ligands were obtained from the medicinally important herbal plants, such as Solanum trilobatum and Hempedu bumi and conjugated with the nanostructures silver nanoparticle and SWCNT, respectively. On the other hand, bio-ligands from cow urine were encapsulated in the cellulose nanoparticle. To confirm morphology these nanostructures, they were observed under Field Emission Scanning Electron Microscope and the results displayed the uniformed nanostructures. Further, biological ligand carrying ability of these nanostructures was confirmed by the bacterial inhibition assay on the agar plate. This study provided the evidence on the capability of nanostructures to carry the biological ligands.
Biotechnology and Applied Biochemistry, Apr 19, 2021
In this work, a nickel ferrite (NiFe2O4)/nitrogen‐doped‐graphene (NG)/cellulose composite (NiFe2O... more In this work, a nickel ferrite (NiFe2O4)/nitrogen‐doped‐graphene (NG)/cellulose composite (NiFe2O4/NG/cellulose) was successfully synthesized through a facile chemical route, and its antibacterial potential was evaluated. The synthesized NiFe2O4/NG/cellulose composite was characterized by performing morphological and structural analyses. The results showed the successful formation of NiFe2O4‐nanoparticles with a spherical shape and a size ranging from 15 to 200 nm. Energy‐dispersive X‐ray results confirmed the presence of various elements (carbon, nitrogen, oxygen, iron, and nickel) in the reaction mixture. The X‐ray diffraction pattern showed the face‐centered‐cubic nature of the particles. In addition, antibacterial activity against Escherichia coli (Gram‐negative bacteria) and Bacillus subtilis (Gram‐positive bacteria) was evaluated with different concentrations of NiFe2O4/NG/cellulose composite (0–50 μg/mL). Inhibitory activity increased with increasing concentrations of NiFe2O4/NG/cellulose. The composite's inhibitory activity was slightly higher in E. coli than in B. subtilis due to the differing nature of their cell wall structures. Overall, the chemically synthesized NiFe2O4/NG/cellulose composite has the potential as an efficient antibacterial agent for controlling the growth of pathogenic bacteria.
International Journal of Biological Macromolecules, May 1, 2013
A total of 18 protease producing bacterial strains were isolated from detergent effluent in South... more A total of 18 protease producing bacterial strains were isolated from detergent effluent in South Korea using skim milk agar medium. A strain (BK-P21A) was selected and identified as Bacillus koreensis based on morphological, biochemical and molecular characterizations (16S rRNA gene sequence analysis). Optimized culture conditions for the production of protease were pH 8.5, 30 °C, sucrose (2%) and yeast extract (0.2%) during 36 h of incubation. Furthermore, the protease was partially purified by ammonium sulphate precipitation (80%) and again by Superdex 200 10/300 GL and Superdex 75 10/300 GL column chromatography, which resulted in 5.0 fold purification and a yield of 23%. The molecular mass of the protease was estimated to be 48 kDa by SDS-PAGE. The purified enzyme was further characterized and found to be most active at pH 9.0 and 60 °C. The activity of the purified protease was enhanced by CaCl₂ and CoCl₂, but inhibited by PMSF, which indicated it was a serine type protease. Moreover, the protease was moderately stable in surfactants and 81% stable in H₂O₂. Finally, the enzyme was more active and stable (94-126.5%) in various hydrophilic organic solvents. Considering the stability of protease towards the alkaline pH, high temperature and organic solvents (50%), the enzyme from B. koreensis can be used as an alternative biocatalyst for several industrial applications mainly for peptide synthesis in nonaqueous solvents.
The fast growing trees were had the acceptable growth, chemical composition and morphological pro... more The fast growing trees were had the acceptable growth, chemical composition and morphological properties that make it suitable for ethanol recovery. The growth attained average level from 1.57m to 3.73m at the end of ninth month. The chemical composition such as higher proportion of holocellulose and lower content of lignin of the fast growing trees expected the more ethanol recovery with minimum recalcitrant. Due to long fiber length, wider fiber width, and wide lumen diameter with thin cell wall thickness of the fast growing trees were preferable for ethanol conversion.
The short rotation trees had longer fiber length, higher slenderness ratio, flexibility coefficie... more The short rotation trees had longer fiber length, higher slenderness ratio, flexibility coefficient and lower Runkel ratio and rigidity coefficient with higher proportion of holocellulose content acceptable lignin, ash and pentasons. These preporties directly related to the high burst strength, tensile strength, tearing strength and folding endurance of the paper. The greatest proportion of fiber length in M. dubia (1022.36μm) and the lowest in Eucalyptus clones (538.80μm). The higher content of holocellulose in A. fraxinifolis (74.2%), P.deltoides (74.15%) and M.dubia (74%) species were recorded and significantly low value in L. leucocephala (70.81%). So, the short rotation trees were more preferable for paper production based on the morphological and chemical properties.
The relevance of maternal macronutrient deficiency on developmental origin of health and adult di... more The relevance of maternal macronutrient deficiency on developmental origin of health and adult disorders has been well studied but not that of micronutrients. We hypothesized that chronic maternal dietary mineral (copper or zinc) or vitamin E restriction modulates the expression of placental 11 beta hydroxysteroid dehydrogenase-2 (11 beta HSD-2) per se predisposing the offspring to insulin resistance (IR) and hypertension in later life. Female weaning Swiss albino mice received a control or a 50% of Vitamin-E or Zn or Cu restricted diet and mated with control males. Pups born to the dams on the restricted diet had significantly (P < 0.001) reduced body weight and crown rump length. These offsprings were weaned on to the restricted diet till postnatal day 180. Glucose intolerance in association with hyperinsulinemeia (IR), hyperlipidemeia and increased systolic blood pressure were recorded in all the offsprings of micronutrient restricted groups. Placental 11 beta HSD-2 expression was attenuated, while activities of glucocorticoid -insensitive enzymes were unchanged in all the restricted groups. Thus, the present study reiterates the importance of micronutrients during pregnancy because chronic maternal micronutrient deficiency may alter placental 11 beta HSD-2 expression and predispose the offspring to IR and hypertension in later life.
Journal of Industrial Microbiology & Biotechnology, Feb 1, 2014
We previously completed whole-genome sequencing of a rare actinomycete named Sebekia benihana, an... more We previously completed whole-genome sequencing of a rare actinomycete named Sebekia benihana, and identified the complete S. benihana cytochrome P450 complement (CYPome), including 21 cytochrome P450 hydroxylase (CYP), seven ferredoxin (FD), and four ferredoxin reductase (FDR) genes. Through targeted CY-Pome disruption, a total of 32 S. benihana CYPome mutants were obtained. Subsequently, a novel cyclosporine A region-specific hydroxylase was successfully determined to be encoded by a CYP-sb21 gene by screening the S. benihana CYPome mutants. Here, we report that S. benihana is also able to mediate vitamin D 3 (VD 3) hydroxylation. Among the 32 S. benihana CYPome mutants tested, only a single S. benihana CYP mutant, DCYP-sb3a, failed to show regio-specific hydroxylation of VD 3 to 25-hydroxyvitamin D 3 and 1a,25-dihydroxyvitamin D 3. Moreover, the VD 3 hydroxylation activity in the DCYP-sb3a mutant was restored by CYP-sb3a gene complementation. Since all S. benihana FD and FDR disruption mutants maintained VD 3 hydroxylation activity, we conclude that CYP-sb3a, a member of the bacterial CYP107 family, is the only essential component of the in vivo regiospecific VD 3 hydroxylation process in S. benihana. Expression of the CYP-sb3a gene exhibited VD 3 hydroxylation in the VD 3 non-hydroxylating Streptomyces coelicolor, implying that the regio-specific hydroxylation of VD 3 is carried out by a specific P450 hydroxylase in S. benihana. Keywords Sebekia benihana Á Vitamin D 3 Á Cytochrome P450 hydroxylation Á Rare actinomycetes J.-G. Ban and H.-B. Kim contributed equally to this paper. Special issue: Genome Mining for Natural Products Discovery.
The aim of this research is to study the morphological analysis of fabricated Interdigitated Elec... more The aim of this research is to study the morphological analysis of fabricated Interdigitated Electrode (IDE). This device electrode was physically characterized using 3D nano profiler, scanning electrode microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX) and Atomic Force Microscope (AFM). Based on this analysis, IDE pattern was analyzed thoroughly based on the IDE pattern specifications with 5 μM finger gap and this research significantly will stand as a platform quantify the biomolecules in further analysis.
Biotechnology and Applied Biochemistry, Mar 1, 2013
The novel protease from Exiguobacterium profundum BK-P23 was partially purified by ammonium sulfa... more The novel protease from Exiguobacterium profundum BK-P23 was partially purified by ammonium sulfate precipitation and further purified Mono Q 5/50 and Superdex 200 10/300 column chromatography. The enzyme was purified 10.23-fold with a yield of 14%. The molecular weight was estimated to be 52 kDa by SDS-PAGE. The enzyme was most active at a pH of 8.0 and temperature of 40 • C and the enzyme was stable between a pH of 7 and 10 and up to a temperature of 50 • C. The enzyme activity was enhanced by CaCl 2 but was slightly inhibited by CoCl 2 , MgSO 4 , and AgNO 3. In addition, this enzyme was completely inhibited by phenylmethylsulfonyl fluoride, indicating that this enzyme was a serine protease. Furthermore, the alkaline protease was more stable in the presence of surfactants such as Triton X-100, which was followed by Tween 80 and SDS. Moreover, the enzyme was highly stable in the presence of 1% oxidizing agent (H 2 O 2). The enzyme also has significant stability (70%-80%) in a few organic solvents. Thus, the increased stability of the enzyme in the presence of oxidizing agent, surfactants, and organic solvents may find potential applications in the detergent industry and peptide synthesis in nonaqueous media.
Journal of The Taiwan Institute of Chemical Engineers, Apr 1, 2021
Abstract Diagnosing gastric cancer with a high accuracy helps in identifying at early stage and f... more Abstract Diagnosing gastric cancer with a high accuracy helps in identifying at early stage and for a better treatment. A transcription factor, SRY-box containing gene-17 (SOX-17) shows a crucial role in the gastric cancer progression is desired as biomarker on a high-performance sensing platform, dimicroelectrodes Junction (DMEJ), constructed by the hybrid of single-walled carbon nanotube (SWCN) and gold urchin (GU) surface to detect the specific SOX-17 target oligonucleotide. SWCN was modified on DMEJ and GU conjugated capture DNA was immobilized on SWCN through the electrostatic interaction of GU by (3-Aminopropyl)triethoxysilane. Morphological analyses by FESEM and FETEM revealed the attachment of GU on SWCN with a uniform fashion. SWCN-GU modified DMEJ has enhanced an ideal electric flow and reflecting the capture DNA attachment. Reached the limit of detection at 1 aM, calculated on a linear curve (atto- to femto-molar) with the regression coefficient of R2 = 0.9909[y = 1.1226x + 1.3727]. Further, specificity of SOX-17 was confirmed by interacting complementary, single- and five-mismatched sequences of the target. In addition, the target DNA solution mixed human serum and control sequences did not interfere with the specific duplex formation, indicating the non-biofouling on DMEJ surface. This SWCN-GU constructed DMEJ surface helps to identify lower expression of SOX-17 and diagnosing gastric cancer progression.
The present study aimed to characterize CoNPs that were synthesized using a simple, inexpensive, ... more The present study aimed to characterize CoNPs that were synthesized using a simple, inexpensive, and eco-friendly way from algal-extract of Elodea canadensis. Further, this study also assessed the antimicrobial potential of the synthesized CoNPs. The CoNPs formation was preliminarily proved through colour change from light-pink to brown within 15 min upon adding the algal-extract to the cobalt (II) nitrate solution. The maximum absorption peaks at 450 nm for the algae-encapsulated CoNPs were obtained using UV-Vis spectrum. FTIR result proved that CoNPs were capped with active components from E. canadensis as distinct peaks were observed at 3288.4 and 1641.8 cm−1 in the infrared spectra of the algal-extract and bio-reduced CoNPs. A FE-SEM revealed that CoNPs were agglomerated and had smooth surfaces. In addition, FE-TEM indicated that the synthesized CoNPs had assorted sizes and shapes. Meanwhile, the XRD displayed several broad peaks at 2θ = 52.37°, 54.21°, and 56.09° with mean size of 14.022 nm that affirmed the crystalline nature of algal synthesized CoNPs. The antimicrobial potential of the biosynthesized CoNPs was assessed using the disc-diffusion test.
Oncogene-mutated breakpoint cluster region protein gene (BCR/ABL) has been found in chronic myelo... more Oncogene-mutated breakpoint cluster region protein gene (BCR/ABL) has been found in chronic myelogenous leukemia (CML) patients. In particular, b3a2 is a common mutation type with CML, tested in this research as deoxyribonucleic acid sensor to identify the specific b3a2 sequence by a suitable capture probe. The aminated capture probe was attached on interdigitated electrode surface through the chemical linker, glycidyloxypropyltrimethoxysilane. Covalently linked reduced graphene oxide (rGO) with target sequence was identified by the capture probe. The prepared rGO from vacuum-assisted low-temperature exfoliated graphite was characterized by field emission scanning electron microscope, field emission transmission electron microscope, energy-dispersive X-ray spectroscopy and fourier-transform infrared spectroscopy. The limit of detection with duplex formation was attained between 1 and 10 aM, and the saturation point was noticed from 10 fM. The sensitivity was calculated to be 1 aM. Further, control experiments with single-, triple-and complete mismatch-sequences instead of target complementary failed to yield the changes in current signal indicating the selective identification of targeted b3a2 sequence. This method of detection helps in identifying the minimal leukemia cells residual with CML patient.
� Using Chinese joss paper silica-aluminium nanocomposite produced by alkalinization. � Character... more � Using Chinese joss paper silica-aluminium nanocomposite produced by alkalinization. � Characterized the silica and aluminium nanocomposite and purity was checked. � Nanocrystal synthesized �15 nm size & spherical. Ratio is 13.24 to 7.96 � Band gap of nanocomposite is 3.84 eV. � Optically measured agglomeration is 36.74 μm, 3D-nanoprofiler height is 234.37 μm.
Different nanostructures were engineered with the nanoscale dimension lesser than 100 nm. These n... more Different nanostructures were engineered with the nanoscale dimension lesser than 100 nm. These nanostructures include silver, cellulose nanoparticles and single-walled carbon nanotube (SWCNT). Biological ligands were obtained from the medicinally important herbal plants, such as Solanum trilobatum and Hempedu bumi and conjugated with the nanostructures silver nanoparticle and SWCNT, respectively. On the other hand, bio-ligands from cow urine were encapsulated in the cellulose nanoparticle. To confirm morphology these nanostructures, they were observed under Field Emission Scanning Electron Microscope and the results displayed the uniformed nanostructures. Further, biological ligand carrying ability of these nanostructures was confirmed by the bacterial inhibition assay on the agar plate. This study provided the evidence on the capability of nanostructures to carry the biological ligands.
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