All articles which are published, including editorials, letters and book reviews represent the op... more All articles which are published, including editorials, letters and book reviews represent the opinion of the authors and are not necessarily those of the Malaysian Medical Association unless otherwise expressed.
Proceedings for Annual Meeting of The Japanese Pharmacological Society
Purpose: Stachytarpheta jamaicensis (L.) Vahl has long been used in traditional medicines, but it... more Purpose: Stachytarpheta jamaicensis (L.) Vahl has long been used in traditional medicines, but its role in the protection of endothelial cells from ROS damage has not yet been established. This study aimed to investigate protective effect of an ethyl acetate extract of Stachytarpheta jamaicensis (L.) Vahl (EASJ) on human umbilical vein endothelial cells (HUVECs) under oxidative stress induced by H2O2. Methods: HUVECs were pre-treated with EASJ prior to H2O2 stimulation. Cell viability was evaluated by 3,(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Endothelial permeability assay was examined using fluorescein isothiocyanate (FITC)-dextran. ROS generation in H2O2-stimulated HUVECs was evaluated by the fluorescence intensity of 2',7'-Dichlorofluorescin diacetate (DCFH-DA). Additionally, the effect of EASJ on F-actin rearrangement and VE-cadherin distribution were assessed using immunofluorescence staining. Result(s): The EASJ concentrations at 10, 50 and 75 ug/mL yielded cell viability higher than 80%. Pre-treatment with EASJ at 75 ug/mL demonstrated anti-inflammatory activity through suppression of endothelial hyperpermeability to 89.12 % of control and attenuated the excessive ROS production to 73.73%. Using an image analyser, reduced formation of stress fibers in actin cytoskeleton and tightening of VE-cadherin in H2O2-induced HUVECs were also observed. Conclusion(s): These findings show that EASJ at 75 ug/mL has a protective effect on HUVECs against hydrogen peroxide-induced damage. This approach may serve as an alternative for the treatment of vascular diseases associated with oxidative stress.
Evidence-Based Complementary and Alternative Medicine, 2016
Introduction.Stachytarpheta jamaicensis(L.) Vahl belongs to the family of Verbenaceae and is comm... more Introduction.Stachytarpheta jamaicensis(L.) Vahl belongs to the family of Verbenaceae and is commonly known as Gervao, Brazilian tea, verbena cimarrona, rooter comb, or blue porter weed. It is one of the important plants with high medicinal and nutraceutical benefits.S. jamaicensiscontains various medicinal properties in traditional and folk medicinal systems, with cures for several diseases.Objective. The objective of this review paper is to collect information concerning the morphology, distribution, traditional usage, phytochemical compositions, biological activities, and safety data ofS. jamaicensis.Materials and Methods. The information was obtained from literature search through electronic databases such as PubMed and Google Scholar onS. jamaicensis.Results and Conclusion. The high medicinal properties of this plant, for instance, antimicrobial and antifungal effect as the main activities, but verbascoside as the main active chemical component, make it a valuable source of the...
All articles which are published, including editorials, letters and book reviews represent the op... more All articles which are published, including editorials, letters and book reviews represent the opinion of the authors and are not necessarily those of the Malaysian Medical Association unless otherwise expressed.
Proceedings for Annual Meeting of The Japanese Pharmacological Society
Purpose: Stachytarpheta jamaicensis (L.) Vahl has long been used in traditional medicines, but it... more Purpose: Stachytarpheta jamaicensis (L.) Vahl has long been used in traditional medicines, but its role in the protection of endothelial cells from ROS damage has not yet been established. This study aimed to investigate protective effect of an ethyl acetate extract of Stachytarpheta jamaicensis (L.) Vahl (EASJ) on human umbilical vein endothelial cells (HUVECs) under oxidative stress induced by H2O2. Methods: HUVECs were pre-treated with EASJ prior to H2O2 stimulation. Cell viability was evaluated by 3,(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Endothelial permeability assay was examined using fluorescein isothiocyanate (FITC)-dextran. ROS generation in H2O2-stimulated HUVECs was evaluated by the fluorescence intensity of 2',7'-Dichlorofluorescin diacetate (DCFH-DA). Additionally, the effect of EASJ on F-actin rearrangement and VE-cadherin distribution were assessed using immunofluorescence staining. Result(s): The EASJ concentrations at 10, 50 and 75 ug/mL yielded cell viability higher than 80%. Pre-treatment with EASJ at 75 ug/mL demonstrated anti-inflammatory activity through suppression of endothelial hyperpermeability to 89.12 % of control and attenuated the excessive ROS production to 73.73%. Using an image analyser, reduced formation of stress fibers in actin cytoskeleton and tightening of VE-cadherin in H2O2-induced HUVECs were also observed. Conclusion(s): These findings show that EASJ at 75 ug/mL has a protective effect on HUVECs against hydrogen peroxide-induced damage. This approach may serve as an alternative for the treatment of vascular diseases associated with oxidative stress.
Evidence-Based Complementary and Alternative Medicine, 2016
Introduction.Stachytarpheta jamaicensis(L.) Vahl belongs to the family of Verbenaceae and is comm... more Introduction.Stachytarpheta jamaicensis(L.) Vahl belongs to the family of Verbenaceae and is commonly known as Gervao, Brazilian tea, verbena cimarrona, rooter comb, or blue porter weed. It is one of the important plants with high medicinal and nutraceutical benefits.S. jamaicensiscontains various medicinal properties in traditional and folk medicinal systems, with cures for several diseases.Objective. The objective of this review paper is to collect information concerning the morphology, distribution, traditional usage, phytochemical compositions, biological activities, and safety data ofS. jamaicensis.Materials and Methods. The information was obtained from literature search through electronic databases such as PubMed and Google Scholar onS. jamaicensis.Results and Conclusion. The high medicinal properties of this plant, for instance, antimicrobial and antifungal effect as the main activities, but verbascoside as the main active chemical component, make it a valuable source of the...
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