Papers by Paweł Krysiński
Analytical and Bioanalytical Chemistry, Dec 16, 2012
Nanomaterials, Feb 26, 2022
Magnetochemistry
In this work, we present magnetic nanoparticles based on iron oxide doped with zinc synthesized u... more In this work, we present magnetic nanoparticles based on iron oxide doped with zinc synthesized using the wet co-precipitation method for environmental application. The morphology of the samples was revealed by SEM and TEM, which showed particles of granular shape and size of about 15 nm. The specific surface areas of the materials using the BET method were within the range of 85.7 to 101.5 m2 g−1 depending on the zinc content in the superparamagnetic iron oxide nanoparticles (SPIONs). Magnetometry was performed to determine the magnetic properties of the particles, indicating superparamagnetism. Synthesized magnetic nanoparticles with different amounts of zinc dopant were used as an adsorbent to remove model pollutant Titan yellow (TY) from the aqueous solutions. Adsorption was determined by investigating the effects of sorbent amount, dye concentration, and contact time. The synthesized material removed Titan yellow quickly and efficiently within the physical adsorption. The adsor...
Pharmaceutics
Iron oxide nanoparticles are commonly used in many medical applications as they can be easily mod... more Iron oxide nanoparticles are commonly used in many medical applications as they can be easily modified, have a high surface-to-volume ratio, and are biocompatible and biodegradable. This study was performed to synthesize nanoparticles designed for multimodal HER2-positive cancer treatment involving radionuclide therapy and magnetic hyperthermia. The magnetic core (Fe3O4) was coated with a gold-198 layer creating so-called core-shell nanoparticles. These were then further modified with a bifunctional PEG linker and monoclonal antibody to achieve the targeted therapy. Monoclonal antibody—trastuzumab was used to target specific breast and nipple HER2-positive cancer cells. The nanoparticles measured by transmission electron microscopy were as small as 9 nm. The bioconjugation of trastuzumab was confirmed by two separate methods: thermogravimetric analysis and iodine-131 labeling. Synthesized nanoparticles showed that they are good heat mediators in an alternating magnetic field and exh...
International Journal of Molecular Sciences
In this paper, the techniques used to study the function of mitochondrial potassium channels are ... more In this paper, the techniques used to study the function of mitochondrial potassium channels are critically reviewed. The majority of these techniques have been known for many years as a result of research on plasma membrane ion channels. Hence, in this review, we focus on the critical evaluation of techniques used in the studies of mitochondrial potassium channels, describing their advantages and limitations. Functional analysis of mitochondrial potassium channels in comparison to that of plasmalemmal channels presents additional experimental challenges. The reliability of functional studies of mitochondrial potassium channels is often affected by the need to isolate mitochondria and by functional properties of mitochondria such as respiration, metabolic activity, swelling capacity, or high electrical potential. Three types of techniques are critically evaluated: electrophysiological techniques, potassium flux measurements, and biochemical techniques related to potassium flux measu...
Nanomaterials, 2022
The study aimed to synthesize and characterize the magnetic drug carrier modified with terbium (I... more The study aimed to synthesize and characterize the magnetic drug carrier modified with terbium (III) ions. The addition of terbium extends the possibilities of their applications for targeted anticancer radiotherapy as well as for imaging techniques using radioisotopes emitting β+, β−, α, and γ radiation. The synthesis of iron oxide nanoparticles stabilized with citrates using the co-precipitation method (IONP @ CA) was carried out during the experimental work. The obtained nanoparticles were used to synthesize a conjugate containing terbium ions and guanosine-5′-monophosphate as an analog of drugs from the thiopurine group. Conjugates and their components were characterized using Transmission Electron Microscopy, infrared spectroscopy, X-ray microanalysis, spectrofluorimetry, and thermogravimetric analysis. The hybrid was also investigated with Langmuir layers to check the interaction with analogs of biological membranes.
Pharmaceutics, 2021
Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biomedical applications f... more Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for biomedical applications for their outstanding properties such as facile functionalization and doping with different metals, high surface-to-volume ratio, superparamagnetism, and biocompatibility. This study was designed to synthesize and investigate multifunctional nanoparticle conjugate to act as both a magnetic agent, anticancer immunological drug, and radiopharmaceutic for anticancer therapy. The carrier, 166Ho doped iron oxide, was coated with an Au layer, creating core-shell nanoparticles ([166Ho] Fe3O4@Au. These nanoparticles were subsequently modified with monoclonal antibody trastuzumab (Tmab) to target HER2+ receptors. We describe the radiobioconjugate preparation involving doping of a radioactive agent and attachment of the organic linker and drug to the SPIONs’ surface. The size of the SPIONs coated with an Au shell measured by transmission electron microscopy was about 15 nm. The bioconjugation of tr...
Nanomaterials, 2020
Comparative photoelectrochemical studies of cadmium sulfide (CdS) nanoparticles with either hydro... more Comparative photoelectrochemical studies of cadmium sulfide (CdS) nanoparticles with either hydrophilic or hydrophobic surface properties are presented. Oleylamine organic shells provided CdS nanoparticles with hydrophobic behavior, affecting the photoelectrochemical properties of such nanostructured semiconductor. Hydrophilic CdS nanoparticles were drop-cast on the electrode, whereas the hydrophobic ones were transferred in a controlled manner with Langmuir-Blodgett technique. The substantial hindrance of photopotential and photocurrent was observed for L-B CdS films as compared to the hydrophilic, uncoated nanoparticles that were drop-cast directly on the electrode surface. The electron lifetime in both hydrophilic and hydrophobic nanocrystalline CdS was determined, revealing longer carrier lifetime for oleylamine coated CdS nanoparticles, ascribed to the trapping of charge at the interface of the organic shell/CdS nanoparticle and to the dominant influence of the resistance of th...
Nanomaterials, 2020
Iron oxide-based nanoparticles have been modified in their core with holmium(III) in an amount af... more Iron oxide-based nanoparticles have been modified in their core with holmium(III) in an amount affecting only slightly their magnetic properties. Nanoparticles were conjugated covalently with biomolecule of trastuzumab (Herceptin®), the monoclonal antibody that recognizes cancer cells overexpressing HER2 receptors targeting such nanoparticles to the specified tumor tissues. Systematic studies of Ho3+-doped bioconjugates were carried out as a preliminary step for future replacement of ‘cold’ Ho with 166Ho radionuclide, emitting ‘soft’ beta(-) radiation for possible targeted radionuclide therapy. Physicochemical properties of the obtained bioconjugates were subsequently tested for use in magnetic hyperthermia, considered as an effective, low-invasiveness anticancer therapy. With such a system we expect to achieve both: active targeting and multimodal action by simultaneous internal and localized irradiation and magnetic hyperthermia of specific cancers.
Molecules, 2020
It has been proven and confirmed in numerous repeated tests, that the use of a combination of sev... more It has been proven and confirmed in numerous repeated tests, that the use of a combination of several therapeutic methods gives much better treatment results than in the case of separate therapies. Particularly promising is the combination of ionizing radiation and magnetic hyperthermia in one drug. To achieve this objective, magnetite nanoparticles have been modified in their core with α emitter 225Ac, in an amount affecting only slightly their magnetic properties. By 3-phosphonopropionic acid (CEPA) linker nanoparticles were conjugated covalently with trastuzumab (Herceptin®), a monoclonal antibody that recognizes ovarian and breast cancer cells overexpressing the HER2 receptors. The synthesized bioconjugates were characterized by transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) measurement, thermogravimetric analysis (TGA) and application of 131I-labeled trastuzumab for quantification of the bound biomolecule. The obtained results show that one 225Ac@Fe3O4-C...
Nanomaterials, 2019
The release profiles of methotrexate, an anticancer drug, from the monoolein liquid crystalline c... more The release profiles of methotrexate, an anticancer drug, from the monoolein liquid crystalline cubic phases were studied. The cubic phases were used either in the form of a lipidic film deposited onto a glassy carbon electrode surface or in the dispersed form of magnetocubosomes, which are considered a prospective hybrid drug delivery system. Commonly, cubosomes or liposomes are employed, but not in the case of toxic methotrexate, known to block the receptors responsible for folate transport into the cells. The release profiles of the drug from the lipidic films were monitored electrochemically and described using the Higuchi model. They were also modified via changes in temperature; the release was faster, although it deviated from the model when the temperature was increased. Cubic phase nanoparticles (magnetocubosomes) containing hydrophobic magnetic nanoparticles placed in an alternating magnetic field of low frequency and amplitude, stimulated drug release from the suspension,...
The release profiles of methotrexate, an anticancer drug, from the monoolein liquid crystalline c... more The release profiles of methotrexate, an anticancer drug, from the monoolein liquid crystalline cubic phases were studied. The cubic phases were used either in the form of a lipidic film deposited onto a glassy carbon electrode surface or in the dispersed form of magnetocubosomes, which are considered a prospective hybrid drug delivery system. Commonly, cubosomes or liposomes are employed, but not in the case of toxic methotrexate, known to block receptors responsible for folate transport into the cells. The release profiles of the drug from the lipidic films were monitored electrochemically and described using the Higuchi model. They were also modified via changes in temperature; the release was faster, although deviating from the model when the temperature was increased. Magnetocubosomes - cubic phase nanoparticles containing hydrophobic magnetic nanoparticles placed in an alternating magnetic field of low frequency and amplitude, stimulated drug release from the suspension, which...
ACS applied materials & interfaces, Jan 28, 2016
Hybrid materials consisting of a monoolein lipidic cubic phase (LCP) incorporating two types of m... more Hybrid materials consisting of a monoolein lipidic cubic phase (LCP) incorporating two types of magnetic nanoparticles were designed as addressable drug delivery systems. The materials, prepared in the form of a gel, were subsequently used as a macroscopic layer modifying an electrode and, after dispersion to nanoscale, as magnetocubosomes. These two LCPs were characterized by Small-Angle X-ray Scattering (SAXS), cross-polarized microscopy, magnetic measurements and phase diagrams. The magnetic dopants were hydrophobic NPoleic and hydrophilic NPcitric nanoparticles, which were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM), and their influence on the properties of the cubic phases was investigated. The removal of the anticancer drug, Doxorubicin (Dox) from the hybrid cubic phase gels was studied by electrochemical methods. The advantages of incorporating magnetic nanoparticles into the self-assembled lipid liquid crystalline phases include...
ECS Transactions, 2014
ABSTRACT Renata Bilewicz, Dominika Majdecka, Michal Kizling, Krzysztof Stolarczyk, Paweł Krysińsk... more ABSTRACT Renata Bilewicz, Dominika Majdecka, Michal Kizling, Krzysztof Stolarczyk, Paweł Krysiński, Jerzy Golimowski, Faculty of Chemistry, University of Warsaw Pasteura 1, Warszawa 02-093, Poland The advantages of biobatteries include easy miniaturization and utility for powering small devices e.g. sensors, switches, watches and other electronic devices. Recently, we have shown the biobattery parameters and its application for powering a clock [1]. Our aim was to optimize the construction of the biobattery and couple it with a biosensor for neurotransmitter detection. Arylated multiwall carbon nanotubes with bound laccase are used on the cathode for the reduction of oxygen and zinc disc covered with hopeite is the anode. Arylated carbon nanotubes increase the working surface of the electrode, and provide direct contact with the active sites of laccase. The electrode substrate is carbon paper. The zinc – air sandwich biobattery works under stationary or flow conditions. Parameters and time dependencies of the flow cell were evaluated. The system including the sandwich biobattery connected with the sensor was employed for monitoring selected neurotransmitters. * K. Stolarczyk; M. Kizling; D. Majdecka; K. Zelechowska; J. F Biernat; J. Rogalski; R. Bilewicz „Biobatteries and Biofuel Cells with Biphenylated Carbon Nanotubes” J. Power Sources 2014, 249, 263–269.
The Journal of Physical Chemistry B, 2003
Chemistry - A European Journal, 2011
We report on the preparation of water-filled polymer microvessels through the photopolymerization... more We report on the preparation of water-filled polymer microvessels through the photopolymerization of pyrrole in a water/chloroform emulsion. The resulting structures were characterized by complementary spectroscopic and microscopic techniques, including Raman spectroscopy, XPS, SEM, and TEM. The encapsulation of fluorescent, magnetic, and ionic species within the microvessels has been demonstrated. Confocal microscopy and fluorescence anisotropy measurements revealed that the encapsulated chromophore (Rhodamine 6G) resides within voids in the capsules; however, strong interaction of the dye with polypyrrole results in a measurable decrease in its rotational dynamics. Microvessels loaded with ferrofluid exhibit magnetic properties, and their structures can be directed with an external magnetic field. TEM measurements allowed imaging of individual nanoparticles entrapped within the vessels. The application of Cu(2+)-loaded microvessels as a transducer layer in all-solid-state ion-selective electrodes was also demonstrated.
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Papers by Paweł Krysiński