Papers by Abbas Pardakhty
Advanced Pharmaceutical Bulletin, 2019
Purpose: Combination of benzoyl peroxide (BPO) with topical antibiotics can lead to higherefficac... more Purpose: Combination of benzoyl peroxide (BPO) with topical antibiotics can lead to higherefficacy and less bacterial resistance, but it in turn increases adverse effects such as skinirritability and dryness. In this study, the efficacy of combination therapy of niosomal BPO 1%and clindamycin (CL) 1% is compared with niosomal CL in acne vulgaris.Methods: This is a double-blind clinical trial study on 100 patients with acne vulgaris inAfzalipour hospital in Kerman. Patients were randomly divided into 2 groups (case and control).The case group received niosomal combination of BPO 1% and CL 1%.The control groupreceived niosomal CL1%. The efficacy of treatment protocols was evaluated in 2nd, 4th, 8thand 12th weeks of treatment by counting lesions (severity and grading acne lesions) and qualityof life (QoL). Furthermore, side effect were evaluated at each treatment visits.Results: The reduction in mean percentage of acne lesions in case group (treated with BPO 1%and CL1%) (64.21%) was hi...
Novel Approaches for Drug Delivery
Lipid vesicular systems composed of hydrated amphihiles with or without bilayer inducing agents s... more Lipid vesicular systems composed of hydrated amphihiles with or without bilayer inducing agents such as cholesterol. On the basis of used amphiphilic molecule different nomenclature are used as liposomes, ufasomes and niosomes. Nonionic surfactants with mono-, di- or trialkyl chains form niosomes which are lipid vesicles with more chemical stability in comparison with phospholipids of liposomes. Both hydrophobic and hydrophilic chemicals can be encapsulated in niosomes as a new drug delivery system. This drug carrier system could have administered via injection, oral, pulmonary, vaginal, rectal, ophthalmic, nasal or transdermal routes with penetration enhancing potential. This chapter presents a detailed explain about niosome forming components, methods of preparation and routes of administration. Many examples for drug delivery potential of niosomes are also available in this review. Vaccine adjuvant and genetic substances vector capabilities are not given here.
Journal of Molecular Liquids, 2016
A sensitive modified carbon paste electrode (MCPE) employing NiO nanoparticle (NiO/NPs) and 1-but... more A sensitive modified carbon paste electrode (MCPE) employing NiO nanoparticle (NiO/NPs) and 1-butyl-3methylimidazolium tetrafluoroborate (BMITFB) ionic liquid was used for trace level analysis of hydroquinone (HQ) in aqueous solution. Compared to bare electrode, the electro-oxidation signal of HQ was greatly improved. The obtained result shows that, the electro-oxidation signal was increased to about 2.5 times at the surface of (NiO/NPs/BMITFB/MCPE) compared to CPE. The linear response range and detection limit were found to be 0.1-500 μM and 0.05 μM by square wave voltammetry (SWV), respectively. NiO/NPs/BMITFB/MCPE was effectively applied for the determination of HQ in real samples such as Liposomes carrier containing HQ and water sample.
Journal of Molecular Liquids, 2016
A square wave voltammetric method for the trace analysis of ascorbic acid was developed in this s... more A square wave voltammetric method for the trace analysis of ascorbic acid was developed in this study. Carbon paste electrode was modified with NiO nanoparticle and 1-butyl-3-methylimidazolium tetrafluoroborate as a binder. Electro-oxidation behavior of ascorbic acid on the modified electrode was studied, which indicated that the nanostructure modified electrode could efficiently promote electrocatalytic oxidation of ascorbic acid. A fast, selective, high sensitive and simple electrochemical strategy was then developed for trace analysis of ascorbic acid using the constructed electrode. The catalytic oxidation signal exhibited a wide linear range from 0.08 to 380.0 μM toward the concentration of ascorbic acid with a sensitivity of 0.0158 μA/μM, and the limit of detection was as low as 0.04 μM. The suggested sensor was also used for quantitative determination of ascorbic acid in food and pharmaceutical samples.
Background and Aims: Delivery of drugs to the systemic circulation through colonic absorption rep... more Background and Aims: Delivery of drugs to the systemic circulation through colonic absorption represents a novel mode of introducing enzyme labile drugs such as peptides. In this research the preparation and characterization of novel calcium alginate beads loaded with niosomes was studied. Methods: Rhodamin B (RB) was used as a model substance and encapsulated in sorbitan palmitate (Span 40) niosomes. The niosomal suspension was then mixed with sodium alginate and dropped into CaCl2 solution. The resultant beads were dried at room temperature and coated with Eudragit 100. Morphological study, in vitro dissolution rate measurement and size analysis was evaluated. In vivo passage through rats' GI tract was observed by animal imager. Results: Multi-lamellar vesicles (MLVs) were formed in the presence of RB. No release in SGF was observed and the dissolution data in SIF were best fitted with diffusion-based models. The mean small intestinal transit time was found more than 3.5-4 h and RB was released in the colon about 4.5 h after administration in all rats. This is in conformation with the in vitro RB release studies in SGF and SIF. Conclusions: The results clearly demonstrated that the coated calcium alginate gel beads-entrapped niosome is a potential system for colon-specific drug delivery.
Background and Aims: Different factors such as pH, ionic strength, polarity and dielectric consta... more Background and Aims: Different factors such as pH, ionic strength, polarity and dielectric constant of hydration media could affect on vesicular size. Consequently, several formulation characteristics like release of entrapped material and stability would be changed following size alteration. The present study was designed to evaluate the effect of polyethylene glycol (PEG) incorporation on chlorpheniramine maleate (CPM) niosomal characteristics. Methods: Sorbitan esters (Span 20, 40, 60 and 80), their polyxylated derivatives (Tween 20, 40, 60 and 80) and cholesterol were used for preparation of niosomes by film hydration method. Different molecular weight PEGs from 1500 to 35000D were incorporated in hydration medium to evaluate the volume diameter, drug release profile, CPM encapsulation efficiency and vesicular stability. Results: Round and tubular multilamellar vesicles (MLVs) were formed. Span/Tween 80 could not form vesicles in the presence of PEGs due to unsaturated oleyl alkyl chain in the utilized surfactants. Generally, the PEG chain length increment resulted in CPM encapsulation efficiency and niosomal volume diameter. Vesicular aggregation, cholesterol crystal separation and size change during storage were reduced by incorporation of medium PEG chain length. Conclusions: Formulation of stable vesicular niosomes for entrapment of water soluble molecules such as CPM can be achieved by incorporation of water soluble polymers such as PEGs. Therefore, many factors would be kept in mind for vesicular modification attempts.
BioMed Research International, 2014
The objective of the present study was to prepare a stableivinjectable formulation of ascorbic ac... more The objective of the present study was to prepare a stableivinjectable formulation of ascorbic acid and α-tocopherol in preventing the cerebral ischemia. Different niosomal formulations were prepared by Span and Tween mixed with cholesterol. The physicochemical characteristics of niosomal formulations were evaluatedin vitro. Forin vivoevaluation, the rats were made ischemic by middle cerebral artery occlusion model for 30 min and the selected formulation was used for determining its neuroprotective effect against cerebral ischemia. Neuronal damage was evaluated by optical microscopy and transmission electron microscopy. The encapsulation efficiency of ascorbic acid was increased to more than 84% by remote loading method. The cholesterol content of the niosomes, the hydrophilicity potential of the encapsulated compounds, and the preparation method of niosomes were the main factors affecting the mean volume diameter of the prepared vesicles. High physical stability of the niosomes pre...
Nanomedicine Journal, 2013
Niosomes, non-ionic surfactant vesicles (NSVs), are the hydrated lipids composed mainly of differ... more Niosomes, non-ionic surfactant vesicles (NSVs), are the hydrated lipids composed mainly of different classes of non-ionic surfactants, introduced in the seventies as a cosmetic vehicle. Nowadays, niosomes are used as important new drug delivery systems by many research groups and also they are effective immunoadjuvants which some commercial forms are available in the market. These vesicles recently used as gene transfer vectors as well. This review article presents a brief report about the achievements in the field of nanoscience related to NSVs. Different polar head groups from a vast list of various surfactants with one, two or three lipophilic alkyl, perfluoroalkyl and steroidal moieties may be utilized to form the proper vesicular structures for encapsulating both hydrophilic and hydrophobic compounds. The methods of niosome preparation, the vesicle stability related aspects and many examples of pharmaceutical applications of NSVs will be presented. The routes of administration ...
Journal of Advanced Research, 2019
Recently, carbon dots (CDs) have attracted great attention due to their superior properties, such... more Recently, carbon dots (CDs) have attracted great attention due to their superior properties, such as biocompatibility, fluorescence, high quantum yield, and uniform distribution. These characteristics make CDs interesting for bioimaging, therapeutic delivery, optogenetics, and theranostics. Photoluminescence (PL) properties enable CDs to act as imaging-trackable gene nanocarriers, while cationic CDs with high transfection efficiency have been applied for plasmid DNA and siRNA delivery. In this review, we have highlighted the precursors, structure and properties of positively charged CDs to demonstrate the various applications of these materials for nucleic acid delivery. Additionally, the potential of CDs as trackable gene delivery systems has been discussed. Although there are several reports on cellular and animal approaches to investigating the potential clinical applications of these nanomaterials, further systematic multidisciplinary approaches are required to examine the pharmacokinetic and biodistribution patterns of CDs for potential clinical applications.
Background: Cognitive impairment is one of the most crucial disorders among multiple sclerosis (M... more Background: Cognitive impairment is one of the most crucial disorders among multiple sclerosis (MS) patients. Since MS is an inflammatory disease and Boswellia papyrifera has anti-inflammatory effects, the influence of B. papyrifera on cognitive impairment in MS patients has been investigated in the present study. Methods: In this clinical trial, 80 MS patients who referred to the clinic of Shafa Hospital, Kerman, Iran were selected. Having completed a written consent form, patients with relapsing remitting MS, with no occurrence of a new attack throughout 1 month before the study, with no pregnancy or breastfeeding entered the study. The patients were randomly divided into two groups; then Brief International Cognitive Assessment for MS (BICAMS) test was carried out. One group received B. papyrifera (capsule 300 mg, twice a day) while the other group received placebo with the same dose for 2 months. After 2 months of treatment, BICAMS was redone and changes were analyzed. The signi...
Background: Development of new drug carriers would be an interesting approach if it allowed incre... more Background: Development of new drug carriers would be an interesting approach if it allowed increased efficacy of antibiotics and a reduction in doses, thus reducing the risk of developing resistance. As with most drug carriers, niosomes have been used to improve the selective delivery and the therapeutic index of antimicrobial agents. Methods: In this study, different formulation of niosomes containing ciprofloxacin (CPFX), Span (20, 60 or 80), Tween (20, 60 or 80) and cholesterol were prepared by film hydration method. The release of the drug from different formulations was stud-ied by using Franz diffusion cell. The niosomes were further characterized by optical microscopy and particle size analysis, and their antimicrobial activity was evaluated. Results: Size of the niosomes was significantly dependent on the amount of cholesterol and surfactant type and varied from 8.56 to 61.3 μm. The entrapment efficiency of CPFX niosomes prepared by remote loading was more than 74%. Niosome...
Microencapsulation has become a common technique in the production of controlled release dosage f... more Microencapsulation has become a common technique in the production of controlled release dosage forms. Many results have been reported, concerning the use of alginate beads as controlled release drug formulations. Alginate has a unique gel-forming property in the presence of multivalent cations, in an aqueous medium. Ibuprofen is an excellent analgesic and antipyretic, non-steroidal anti-inflammatory agent with a high therapeutic index. Formulation of ibuprofen in beads could reduce its gastric ulcerogenicity. Hence, in this study the formation of Ca-alginate ibuprofen beads, through ionotropic gelation has been investigated. For this purpose, different cross- linking agents including: Ca2+, Ba2+, Mn2+, Co2+, Sn2+ and Pb2+, were used for bead preparation. Next, characterization of the beads, size distribution, encapsulation efficiency of ibuprofen within the beads, the bead swelling and the drug release kinetic were investigated. Results showed that only Ca ion is suitable for the f...
Objective(s): Exosomes are endogenous nanovesicles act as intercellular communication tools which... more Objective(s): Exosomes are endogenous nanovesicles act as intercellular communication tools which have been considered to utilize as drug delivery systems. As transporting therapeutic molecules into brain has obstacles, preparing exosomes which have the potential to pass through its barriers is great challenge. Methods: Exosomes isolated from cell culture media of U87 glioblastoma cells were characterized. In the next step, paclitaxel (PTX) was loaded into them to investigate the cytotoxicity of this formulation on two cell line of glioblastoma, U87 and T98G. Pharmaceutical characterizations such as size analysis, PTX encapsulation efficiency and FESEM/TEM imaging of exosomes were also evaluated. Results: CD9 as a biomarker of exosomes was detected in extracted samples to confirm the presence of exosomes.Size analysis and electron microscopy imaging proved nano-range of isolated and drug loaded exosomes. The cytotoxicity of empty exosomes of U87 cells was different on U87 and T98 ce...
Introduction: Androgenetic alopecia (AGA) is a common disease in both male and female genders whi... more Introduction: Androgenetic alopecia (AGA) is a common disease in both male and female genders which affect millions of people worldwide. Minoxidil and Aminexil can improve the blood supply of hair follicles by different mechanisms. Here, we report for the first time, the preparation and physicochemical evaluation of aminexil niosomes. Methods and Results: We developed new noisome encapsulated aminexil formulation composed of sorbitan esters (Span™), their ethoxylated derivatives (Tween™) with cholesterol by lipid film hydration method. Four molar ratio were used. The suspension was centrifuged and the absorbance of the supernatant analyzed by UV spectrophotometer at the λ max. The morphological studies of niosomes of aminexil have been done by using transmission electronic microscope (TEM). Size distribution were evaluated by Malvern size analyzer. Release rate of niosomal aminexil was evaluated by Franz diffusion cell through abdominal skin of rat. Results showed that the prepared ...
DARU Journal of Pharmaceutical Sciences, 2011
Background and the purpose of the study Encapsulation of human insulin in lipid vesicular systems... more Background and the purpose of the study Encapsulation of human insulin in lipid vesicular systems such as niosomes was sought as a route to protect this protein against proteolytic enzymes and to improve its oral bioavailability. The purpose of this study was to assess the effect of insulin encapsulation in niosomes on oral bioavailability in diabetic rats. Methods Recombinant human insulin was entrapped in multilamellar niosomes composed of polyoxyethylene alkyl ether surfactants (Brij 52 and Brij 92) or sorbitan monostearate (Span 60) and cholesterol. The amount of insulin released in simulated intestinal fluid (SIF) and simulated gastric fluid (SGF) were measured at 37°C. The protection of entrapped insulin against pepsin, α-chymotrypsin and trypsin were evaluated in comparison with free insulin solution. Diabetes was induced by IP injection of streptozotocin (65 mg/kg) in male wistar rats and effects of orally administered niosomes and subcutaneously injected insulin on hypoglyc...
Purpose: This research aims to analyze and visualize the structure of Iranian scholarly networks ... more Purpose: This research aims to analyze and visualize the structure of Iranian scholarly networks in the field of “pharmacology and pharmacy”. This study includes an overview of co-authorship, efficiency and ranking of the researches, visualizing the co-authorship network, changes in the main core of the publications and macro and micro-level metrics such as social influence. Methods: This research utilizes social network analysis (SNA). The preliminary data of this research includes all the Iran’s documents in Web of Science in “Pharmacology and Pharmacy” during the period of 2005 to 2016. After the preprocessing of 6204 records and creating relational matrix, a combination of bibliometric software (including UCINET, NetDraw, HistCite and VOSviewer) were used to analyze and uncover network features.
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Papers by Abbas Pardakhty