a b s t r a c t 2,4-Dichlorophenol (2,4-DCP) is harmful for aquatic life and human health, so man... more a b s t r a c t 2,4-Dichlorophenol (2,4-DCP) is harmful for aquatic life and human health, so many attempts have focused on removing it through innocuous technologies. Hairy roots (HR) represent an interesting plant system to study the process and to remove efficiently this compound. In the present work, tobacco HR clones were obtained and one of them was selected for 2,4-DCP phytoremediation assays. These cultures removed 2,4-DCP in short time and with high efficiency (98%, 88% and 83%) for solutions initially containing 250, 500 and 1000 mg/L, respectively. Removal process was mainly associated with peroxidase activity. The highest efficiency for 2,4-DCP (500 mg/L) removal was reached at 60 min and using 10 mM H 2 O 2 . Moreover, HR could be re-used, almost for three consecutive cycles. The diminution of pH and the increase of chloride ions in post-removal solutions suggested that 2,4-DCP dehalogenation was mediated by peroxidases. Moreover, changes in deposition pattern of lignin in HR exposed to 2,4-DCP suggested that cell walls of xylem and phloem elements would be the site of deposition of some products formed and they would be a lignin-type polymer. These findings contribute to understand 2,4-DCP removal process with tobacco HR and it might have implications in the use of this system for decontamination of polluted waters.
El presente trabajo estudia la biología reproductiva de la jibia o calamar gigante, Dosidicus gig... more El presente trabajo estudia la biología reproductiva de la jibia o calamar gigante, Dosidicus gigas frente a la costa de Chile central capturados en julio-agosto (invierno) y octubre-noviembre (primavera) de 1993. En 254 hembras (93 de invierno y 161 de primavera) se analizó el ...
Page 1. Revista de Biología Marina y Oceanografía 44(1): 257-262, abril de 2009 Ciclo reproductiv... more Page 1. Revista de Biología Marina y Oceanografía 44(1): 257-262, abril de 2009 Ciclo reproductivo y talla media de madurez del congrio colorado, Genypterus chilensis (Guichenot, 1881) en el litoral de Talcahuano, Chile ...
a b s t r a c t 2,4-Dichlorophenol (2,4-DCP) is harmful for aquatic life and human health, so man... more a b s t r a c t 2,4-Dichlorophenol (2,4-DCP) is harmful for aquatic life and human health, so many attempts have focused on removing it through innocuous technologies. Hairy roots (HR) represent an interesting plant system to study the process and to remove efficiently this compound. In the present work, tobacco HR clones were obtained and one of them was selected for 2,4-DCP phytoremediation assays. These cultures removed 2,4-DCP in short time and with high efficiency (98%, 88% and 83%) for solutions initially containing 250, 500 and 1000 mg/L, respectively. Removal process was mainly associated with peroxidase activity. The highest efficiency for 2,4-DCP (500 mg/L) removal was reached at 60 min and using 10 mM H 2 O 2 . Moreover, HR could be re-used, almost for three consecutive cycles. The diminution of pH and the increase of chloride ions in post-removal solutions suggested that 2,4-DCP dehalogenation was mediated by peroxidases. Moreover, changes in deposition pattern of lignin in HR exposed to 2,4-DCP suggested that cell walls of xylem and phloem elements would be the site of deposition of some products formed and they would be a lignin-type polymer. These findings contribute to understand 2,4-DCP removal process with tobacco HR and it might have implications in the use of this system for decontamination of polluted waters.
El presente trabajo estudia la biología reproductiva de la jibia o calamar gigante, Dosidicus gig... more El presente trabajo estudia la biología reproductiva de la jibia o calamar gigante, Dosidicus gigas frente a la costa de Chile central capturados en julio-agosto (invierno) y octubre-noviembre (primavera) de 1993. En 254 hembras (93 de invierno y 161 de primavera) se analizó el ...
Page 1. Revista de Biología Marina y Oceanografía 44(1): 257-262, abril de 2009 Ciclo reproductiv... more Page 1. Revista de Biología Marina y Oceanografía 44(1): 257-262, abril de 2009 Ciclo reproductivo y talla media de madurez del congrio colorado, Genypterus chilensis (Guichenot, 1881) en el litoral de Talcahuano, Chile ...
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