The anatomy of the edentulous posterior maxilla and maxillary sinus possess unique challenges in ... more The anatomy of the edentulous posterior maxilla and maxillary sinus possess unique challenges in implant dentistry. The purpose of this study was to assess maxillary sinus membrane thickness (MT) and lateral wall thickness (LWT) in different facial index profiles and to describe the clinical implications. A retrospective image analysis of 75 CBCT scans was done, which yielded a total of 150 sinus images. The facial index was calculated as per the formula given in the text and grouped as euryprosopic, mesoprosopic and leptoprosopic. The images obtained were of 36 women (48%) and 39 men (52%), with maximum subjects in 30-39 years age group. MT and LWT were measured at three different points on the radiograph at every 3mm from the base of the sinus floor in premolar and molar regions of each image. Results showed females had significant differences from males in LWT in both premolar and molar regions (p = 0.018 and 0.032 respectively). Subjects in 40-49 years of age had significant differences (p = 0.021) in MT in premolar region only. Also, difference in MT in premolar and molar regions were also statistically significant. Lastly, the present study did not find any statistically significant difference in MT and LWT in all three facial indices groups. It can be concluded that different facial indices have no positive correlation with maxillary sinus membrane thickness and lateral wall thickness. Hence, surgical complications are avoidable with proper detailed knowledge and appropriate identification of the anatomic structures characteristic to the maxillary sinus.
European Journal of Molecular & Clinical Medicine, Nov 23, 2020
Background: Ghrelin is a recently described peptide hormone that is secreted predominantly by the... more Background: Ghrelin is a recently described peptide hormone that is secreted predominantly by the stomach. It induces appetite and thereby controls food intake and energy balance. It has been also observed modulatory effects on the immune system and bone metabolism. Chemerin binds to an orphan G coupled receptor which modulates the innate immune system and chemotaxis of immature dendritic cells and macrophages. Objective: Comparative evaluation of GCF & Serum "levels of Ghrelin and Chemerin" as the (Predictive) biomarkers of inflammation in aggressive periodontitis. Methodology: Total 80 samples will be included and divided into two groups. Group I includes 40 GCF samples from 40 subjects with aggressive and Group II includes 40 Serum samples from 40 subjects with aggressive periodontitis group. The GCF will be collected from the site with deepest probing depth. Gingival index, probing pocket depth and clinical attachment level will be measured one day before GCF collection to avoid stimulation of the sample and its contamination with blood. The Ghrelin and Chemerin levels in GCF and serum samples will be measured using an ELISA kit. Expected Result: The present study will have found correlation the GCF and
European Journal of Molecular & Clinical Medicine, Nov 23, 2020
Background: Osseous resection is established treatment protocol for periodontal hemiseptal defect... more Background: Osseous resection is established treatment protocol for periodontal hemiseptal defects, however this forces clinician to settle for less than optimal results and leads to undesirable outcomes like unpleasant esthetics, denitinal hypersensitivity, root caries etc. the present treatment protocol is designed to asses defect resolution by autogenous bone pieces in treatment of periodontal hemiseptal defects. Objectives: To evaluate the soft and hard tissue response using clinical parameters like probing pocket depth(PPD), clinical attachment level(CAL) and radiographic bone fill in treatment of hemiseptal periodontal defects to Open flap debridement, Osseous resective surgery autogenous bone graft. Methods: the study sample will be randomly divided in to 3 groups of 12 hemiseptal defects each. Group 1 to be treated by resective osseous surgery, Group II treated by open flap debridement and Group III treated by Autogenous bone graft. Clinical parameters include gingival index, probing pocket depth and clinical attachment level along with radiographic assessment with radivisograph (RVG) and cone beam computed tomography (CBCT). All parameters will be evaluated at baseline, 3, 6 and 9 months interval. The Independent-Samples t Test will be done to compare means for two groups." The Paired-Samples t test to compare means of two variables for a single group. Difference will be considered significant when p value <0.05. Expected results: Proposed approach is expected to show improvement in probing pocket depth, clinical attachment level, and gingival index. It is also expected to gain defect fill reduction or resolution of hemiseptal defect. Conclusion : By attempting to treat periodontal hemiseptal defects with regenerative method this study is attempting to avoid all undesired effect associated with osseous resection and provide evidence of regeneration in such defects, this can lead to paradigm shift in treatment approach of periodontal hemiseptal defects.
Aim-To compare the effectiveness of concentrated growth factors enriched bone graft matrix (Stick... more Aim-To compare the effectiveness of concentrated growth factors enriched bone graft matrix (Sticky bone) with hydroxyapatite reinforced Beta TCP and bioabsorbable membrane in treatment of class II furcation defects. Method-Total 24 patients with class II furcation defects were included in the study. The parameters recorded were plaque index (PI), papillary bleeding score (PBI), horizontal probing depth (HPD), vertical probing pocket depth (V-PPD), relative clinical attachment level (R-CAL), relative gingival marginal level(R-GML) on the day of surgery and after 6 months. Results-Comparison between mean PPD reduction between groups at 6 months indicated remarkable reduction in β-TCP + GTR group by 0.83 ± 0.93mm. Similarly, mean R-CAL gain at 6 months indicated notable gain in β-TCP + GTR group by 1 ± 1.27 mm. The mean gain of R-GML (0.25 ± 1.13 mm)and HPD reduction (0.16 ± 1.02 mm)between groups at 6 months showed no statistical signi cance. Conclusion-Sticky bone group showed remarkable improvement from baseline in measured clinical parameters. Percentage of defects converted to class I from class II were higher in sticky bone group (83.3%) compared to HA/β-TCP + GTR group (66.6%) showed regenerative potential which can be compared to combination therapy.
Current research has been more productive for the mechanism of periodontal tissue destruction. Ho... more Current research has been more productive for the mechanism of periodontal tissue destruction. However the transitions from periodontal health to disease has been more elusively intricate and complex of various interactions between resident bacteria and the host. A deeper insight of how the individual putative periodontal pathogens initiate and perpetuate the host response to cause periodontal tissue destruction is more interesting.
PRF was used as a sole grafting material for surgically treatment of a 8 mm pocket around an impl... more PRF was used as a sole grafting material for surgically treatment of a 8 mm pocket around an implant that had 3 threads of bone loss, BoP, and exudate, and the patient was followed up for 1 year. Surgical treatment with the help of PRF, home care reinforcement, clinical indices records, and radiographic examination was done. The patient was monitored frequently for the first 3 months. Subsequently, maintenance visits were scheduled at 3 month intervals. The patient had a decreased probing pocket depth and a negative BoP index compared to initial clinical data, and the results were stable after a year. After 1 year of follow up visit, there appeared to be rebound of the bone level radiographically.
International Journal of Research in Pharmaceutical Sciences, Nov 27, 2020
Coronaviruses was discovered in the mid-1960s that affect humans. In 2019, Wuhan city of Hubei, C... more Coronaviruses was discovered in the mid-1960s that affect humans. In 2019, Wuhan city of Hubei, China, there was an outbreak of "coronavirus disease (COVID-19)" which is the result of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is induced by a novel enveloped virus having single-stranded RNA. It transmitted rapidly affecting more than 200 countries globally, so, the World Health Organization has declared it as a pandemic. SARS-CoV-2 presently is a 7th amongst known coronaviruses that cause infection in people, after 229E and OC43 (earliest studied viruses in human patients suffering from a common cold). It has infected humans in all age groups, of all ethnicities, both males and females while spreading through communities at an alarming rate. Infected patients experience common coldlike symptoms along with raised temperature, non-productive coughing and dif iculty to breathe. It is considered as a relative of severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS), COVID-19 is caused by a beta coronavirus named SARS-CoV-2, which affects the lower respiratory tract. Besides, SARS-CoV-2 also harms different organs. Till today, there is comprehensive knowledge about the extent and management of COVID-19-related disorders other than pulmonary system. The present review is an overview of systemic manifestations of COVID-19 that may affect gastrointestinal, cardiovascular, urinary, reproductive, hepatocellular or neurological systems.
Journal of Datta Meghe Institute of Medical Sciences University, 2019
Periodontitis is initiated by various Gram-negative bacteria characterized by the destruction of ... more Periodontitis is initiated by various Gram-negative bacteria characterized by the destruction of the periodontal tissue. Intracellular proteases, caspases that are the key mediators of apoptosis, are shown to involve in disease progression. The activation of caspase-3 and caspase-7 is considerably enhanced in the gingival tissue from patients with periodontitis. A considerable number of cells in the gingival epithelium and connective tissue revealed active caspases, whereas in healthy tissue, almost no caspase activation was observed. These results therefore suggest that caspase activation may be functionally involved in periodontitis-associated tissue damage.
Journal of the International Clinical Dental Research Organization, 2018
Context: The purpose of this study was to asses oral and periodontal status of the patients with ... more Context: The purpose of this study was to asses oral and periodontal status of the patients with tuberculosis (TB) at various TB hospitals in Vidarbha region of Maharashtra state, India. Aim: The aim of the present cross-sectional observational study was to assess oral and periodontal status in TB patients at four different centers in Vidarbha region of Central India. Settings and Design: This study was a cross-sectional observational study. Subjects and Methods: The study population consisted of 127 patients aged 13–80 years admitted in wards of four different centers around Sawangi. The soft-tissue lesion and periodontal index were assessed using specialized design proforma. Results: None of the patients reported with healthy periodontal status. Oral lesions were observed in 48.03%, with most frequent sites of involvement were tongue, buccal mucosa, and soft palate. A majority of patients had generalized periodontitis. The prevalence of HIV-positive patient was 7.87%. The mean debris score was 1.35 ± 0.66 and mean calculus score was 1.21 ± 0.60 followed by pocket and clinical attachment level in the range of 1.77 ± 0.56 and 2.81 ± 0.71, respectively. Conclusions: The oral health professional should be made aware to treat the oral lesions in patients with TB with greater caution as the incidence of periodontal diseases and oral lesion, and HIV has become alarmingly higher with these classes of patients.
Platelets function as reservoirs for growth-factors and cytokines that are required for soft tiss... more Platelets function as reservoirs for growth-factors and cytokines that are required for soft tissue and bone regeneration, as discovered by Robert Marx in 1971. Choukroun's platelet research resulted in the development of a second-generation platelet concentrate (PRF) in 2001 [8]. The PRF clot forms a strong natural fibrin matrix that has a variety of growth-factors that aid tissue regeneration. The health risks linked with silica containing glass tubes used to collect blood for PRF are still a source of concern. T-PRF was developed to overcome the limitations of PRF [9].
Journal of Datta Meghe Institute of Medical Sciences University, 2018
Gingival enlargement is caused by gingival inflammation, fibrous overgrowth, or combination of bo... more Gingival enlargement is caused by gingival inflammation, fibrous overgrowth, or combination of both, leading to adverse consequences, such as difficulty in plaque control, mastication, altered speech, and esthetic and psychological problems. A 19-year-old male reported with the chief complaint of difficulty in mastication due to swelling of the gingiva for the past 6 months that bled profusely on slight provocation. The lesion gradually increased in size and similar lesions developed in other areas of the jaw, leading to unusual generalized massive gingival enlargement. Within a month of nonsurgical therapy which included supragingival and subgingival scaling, followed by oral hygiene instructions, enlargement reduced significantly. Complete resolution of the inflammatory gingival enlargement was observed after 3 months of nonsurgical therapy without any surgical intervention. Therefore, it will not be an exaggeration to say that scaling is still the gold standard treatment modality in the treatment of plaque-induced gingival enlargement.
Background: In this evolutionary era of dentistry, newer techniques have evolved for complete cov... more Background: In this evolutionary era of dentistry, newer techniques have evolved for complete coverage of multiple recession defects (MRDs). Since 2012, MRDs have been treated using the vestibular incision subperiosteal tunnel access (VISTA) approach and a variety of regenerating membranes with varying degrees of success. Platelet‑rich fibrin matrix (PRFM) membrane has shown a robust release of growth factors and was known to enhance the healing process. Thus, in this study we have evaluated the effectiveness of platelet-rich fibrin matrix (PRFM) with that of platelet-rich fibrin (PRF) membrane using the vestibular incision subperiosteal tunnel access (VISTA) technique in Miller Class I and II multiple gingival recession defects. Methods: This randomized, parallel designed, controlled, clinical study was conducted in 20 subjects (10 male and 10 female) having class I/II MGR defects either buccally or labially. The test group was treated with VISTA and PRFM membrane while the control...
Background Nanotechnology delivers a novel mode for restricting alveolar infections. The particle... more Background Nanotechnology delivers a novel mode for restricting alveolar infections. The particles intermingle through the bacterial cell wall improving antimicrobial action. Its amalgamation and glazing displayed greater germicidal properties. Arraying nanoparticles show sustained delivery of bactericidal property which ultimately aims the dentinal matrix. This study aimed to in vitro appraise the physical properties, antimicrobial, cytotoxicity, time-kill assay, anti-biofilm formation, and anti-inflammatory and antioxidant activity of Cu nanoparticle gel against periodontal pathogens. Materials & Methods Copper nanoparticles (procured from the Nano Research Laboratory, Jharkhand, India) gel was prepared and assessed for minimum inhibitory and minimum bactericidal concentration, MTT assay (cytotoxicity against periodontal and mouse fibroblasts), and time-kill assay at different time intervals against periodontal pathogens. Antibiofilm formation by the cell culture method, anti-infl...
Background: Amongst the specific plaque pathogen Aggregatibacter actinomycetemcomitans (Aa) ATCC ... more Background: Amongst the specific plaque pathogen Aggregatibacter actinomycetemcomitans (Aa) ATCC 43718 serotype b is one of the highly virulent bacteria that causes periodontitis. Probiotic therapy is a treatment in which the lactic acid bacteria in are utilized to impede the colonization and growth of the pathogenic bacteria to prevent the further formation of dental plaque. Objective: The present research aimed to evaluate inhibiting effect of purified bacteria from various commercially available yogurt product containing bacteria named (Lactobacillus casei strain Shirota; Lactobacillus bulgaricus and Streptococcus thermophilus; Lactobacillus reuteri Prodentis) on the growth of Aa. Methods: The research made use of the diffusion method by fixing Aa on BHIB (brain heart infusion broth) medium, incubated at 37°C and 24 hours later planted on MHA (Mueller-Hinton agar) media. Aa were divided into four subgroups each with a paper disk; group 1 consists of untreated bacteria (i.e., control group), group 2 with purified bacteria from Yakult 0.5 µL, group 3 with purified bacteria from Cimory Yogurt Drink 0.5 µL and group 4 with purified bacteria from BioGaia Prodentis 0.5 µL. All commercially available yogurt were treated to get the purified probiotic. Additionally, it was incubated for 24 hours at 37°C and later the inhibition zone diameter was observed. Results: In the research, it was found that the average impeding ability, so-called inhibition zone, in group 1 indicated 0 mm, group 2 indicated 12.70 mm, group 3 indicated 16.60 mm and group 4 indicated 19.60 mm. The statistical test outcomes showed a significance of 0.000 (p < 0.05). Conclusions: The purified bacteria from three probiotics indeed inhibit the growth of the Aa bacteria and a substantial difference in the diameter of the inhibition zone were found among the three probiotics.
The anatomy of the edentulous posterior maxilla and maxillary sinus possess unique challenges in ... more The anatomy of the edentulous posterior maxilla and maxillary sinus possess unique challenges in implant dentistry. The purpose of this study was to assess maxillary sinus membrane thickness (MT) and lateral wall thickness (LWT) in different facial index profiles and to describe the clinical implications. A retrospective image analysis of 75 CBCT scans was done, which yielded a total of 150 sinus images. The facial index was calculated as per the formula given in the text and grouped as euryprosopic, mesoprosopic and leptoprosopic. The images obtained were of 36 women (48%) and 39 men (52%), with maximum subjects in 30-39 years age group. MT and LWT were measured at three different points on the radiograph at every 3mm from the base of the sinus floor in premolar and molar regions of each image. Results showed females had significant differences from males in LWT in both premolar and molar regions (p = 0.018 and 0.032 respectively). Subjects in 40-49 years of age had significant differences (p = 0.021) in MT in premolar region only. Also, difference in MT in premolar and molar regions were also statistically significant. Lastly, the present study did not find any statistically significant difference in MT and LWT in all three facial indices groups. It can be concluded that different facial indices have no positive correlation with maxillary sinus membrane thickness and lateral wall thickness. Hence, surgical complications are avoidable with proper detailed knowledge and appropriate identification of the anatomic structures characteristic to the maxillary sinus.
European Journal of Molecular & Clinical Medicine, Nov 23, 2020
Background: Ghrelin is a recently described peptide hormone that is secreted predominantly by the... more Background: Ghrelin is a recently described peptide hormone that is secreted predominantly by the stomach. It induces appetite and thereby controls food intake and energy balance. It has been also observed modulatory effects on the immune system and bone metabolism. Chemerin binds to an orphan G coupled receptor which modulates the innate immune system and chemotaxis of immature dendritic cells and macrophages. Objective: Comparative evaluation of GCF & Serum "levels of Ghrelin and Chemerin" as the (Predictive) biomarkers of inflammation in aggressive periodontitis. Methodology: Total 80 samples will be included and divided into two groups. Group I includes 40 GCF samples from 40 subjects with aggressive and Group II includes 40 Serum samples from 40 subjects with aggressive periodontitis group. The GCF will be collected from the site with deepest probing depth. Gingival index, probing pocket depth and clinical attachment level will be measured one day before GCF collection to avoid stimulation of the sample and its contamination with blood. The Ghrelin and Chemerin levels in GCF and serum samples will be measured using an ELISA kit. Expected Result: The present study will have found correlation the GCF and
European Journal of Molecular & Clinical Medicine, Nov 23, 2020
Background: Osseous resection is established treatment protocol for periodontal hemiseptal defect... more Background: Osseous resection is established treatment protocol for periodontal hemiseptal defects, however this forces clinician to settle for less than optimal results and leads to undesirable outcomes like unpleasant esthetics, denitinal hypersensitivity, root caries etc. the present treatment protocol is designed to asses defect resolution by autogenous bone pieces in treatment of periodontal hemiseptal defects. Objectives: To evaluate the soft and hard tissue response using clinical parameters like probing pocket depth(PPD), clinical attachment level(CAL) and radiographic bone fill in treatment of hemiseptal periodontal defects to Open flap debridement, Osseous resective surgery autogenous bone graft. Methods: the study sample will be randomly divided in to 3 groups of 12 hemiseptal defects each. Group 1 to be treated by resective osseous surgery, Group II treated by open flap debridement and Group III treated by Autogenous bone graft. Clinical parameters include gingival index, probing pocket depth and clinical attachment level along with radiographic assessment with radivisograph (RVG) and cone beam computed tomography (CBCT). All parameters will be evaluated at baseline, 3, 6 and 9 months interval. The Independent-Samples t Test will be done to compare means for two groups." The Paired-Samples t test to compare means of two variables for a single group. Difference will be considered significant when p value <0.05. Expected results: Proposed approach is expected to show improvement in probing pocket depth, clinical attachment level, and gingival index. It is also expected to gain defect fill reduction or resolution of hemiseptal defect. Conclusion : By attempting to treat periodontal hemiseptal defects with regenerative method this study is attempting to avoid all undesired effect associated with osseous resection and provide evidence of regeneration in such defects, this can lead to paradigm shift in treatment approach of periodontal hemiseptal defects.
Aim-To compare the effectiveness of concentrated growth factors enriched bone graft matrix (Stick... more Aim-To compare the effectiveness of concentrated growth factors enriched bone graft matrix (Sticky bone) with hydroxyapatite reinforced Beta TCP and bioabsorbable membrane in treatment of class II furcation defects. Method-Total 24 patients with class II furcation defects were included in the study. The parameters recorded were plaque index (PI), papillary bleeding score (PBI), horizontal probing depth (HPD), vertical probing pocket depth (V-PPD), relative clinical attachment level (R-CAL), relative gingival marginal level(R-GML) on the day of surgery and after 6 months. Results-Comparison between mean PPD reduction between groups at 6 months indicated remarkable reduction in β-TCP + GTR group by 0.83 ± 0.93mm. Similarly, mean R-CAL gain at 6 months indicated notable gain in β-TCP + GTR group by 1 ± 1.27 mm. The mean gain of R-GML (0.25 ± 1.13 mm)and HPD reduction (0.16 ± 1.02 mm)between groups at 6 months showed no statistical signi cance. Conclusion-Sticky bone group showed remarkable improvement from baseline in measured clinical parameters. Percentage of defects converted to class I from class II were higher in sticky bone group (83.3%) compared to HA/β-TCP + GTR group (66.6%) showed regenerative potential which can be compared to combination therapy.
Current research has been more productive for the mechanism of periodontal tissue destruction. Ho... more Current research has been more productive for the mechanism of periodontal tissue destruction. However the transitions from periodontal health to disease has been more elusively intricate and complex of various interactions between resident bacteria and the host. A deeper insight of how the individual putative periodontal pathogens initiate and perpetuate the host response to cause periodontal tissue destruction is more interesting.
PRF was used as a sole grafting material for surgically treatment of a 8 mm pocket around an impl... more PRF was used as a sole grafting material for surgically treatment of a 8 mm pocket around an implant that had 3 threads of bone loss, BoP, and exudate, and the patient was followed up for 1 year. Surgical treatment with the help of PRF, home care reinforcement, clinical indices records, and radiographic examination was done. The patient was monitored frequently for the first 3 months. Subsequently, maintenance visits were scheduled at 3 month intervals. The patient had a decreased probing pocket depth and a negative BoP index compared to initial clinical data, and the results were stable after a year. After 1 year of follow up visit, there appeared to be rebound of the bone level radiographically.
International Journal of Research in Pharmaceutical Sciences, Nov 27, 2020
Coronaviruses was discovered in the mid-1960s that affect humans. In 2019, Wuhan city of Hubei, C... more Coronaviruses was discovered in the mid-1960s that affect humans. In 2019, Wuhan city of Hubei, China, there was an outbreak of "coronavirus disease (COVID-19)" which is the result of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which is induced by a novel enveloped virus having single-stranded RNA. It transmitted rapidly affecting more than 200 countries globally, so, the World Health Organization has declared it as a pandemic. SARS-CoV-2 presently is a 7th amongst known coronaviruses that cause infection in people, after 229E and OC43 (earliest studied viruses in human patients suffering from a common cold). It has infected humans in all age groups, of all ethnicities, both males and females while spreading through communities at an alarming rate. Infected patients experience common coldlike symptoms along with raised temperature, non-productive coughing and dif iculty to breathe. It is considered as a relative of severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS), COVID-19 is caused by a beta coronavirus named SARS-CoV-2, which affects the lower respiratory tract. Besides, SARS-CoV-2 also harms different organs. Till today, there is comprehensive knowledge about the extent and management of COVID-19-related disorders other than pulmonary system. The present review is an overview of systemic manifestations of COVID-19 that may affect gastrointestinal, cardiovascular, urinary, reproductive, hepatocellular or neurological systems.
Journal of Datta Meghe Institute of Medical Sciences University, 2019
Periodontitis is initiated by various Gram-negative bacteria characterized by the destruction of ... more Periodontitis is initiated by various Gram-negative bacteria characterized by the destruction of the periodontal tissue. Intracellular proteases, caspases that are the key mediators of apoptosis, are shown to involve in disease progression. The activation of caspase-3 and caspase-7 is considerably enhanced in the gingival tissue from patients with periodontitis. A considerable number of cells in the gingival epithelium and connective tissue revealed active caspases, whereas in healthy tissue, almost no caspase activation was observed. These results therefore suggest that caspase activation may be functionally involved in periodontitis-associated tissue damage.
Journal of the International Clinical Dental Research Organization, 2018
Context: The purpose of this study was to asses oral and periodontal status of the patients with ... more Context: The purpose of this study was to asses oral and periodontal status of the patients with tuberculosis (TB) at various TB hospitals in Vidarbha region of Maharashtra state, India. Aim: The aim of the present cross-sectional observational study was to assess oral and periodontal status in TB patients at four different centers in Vidarbha region of Central India. Settings and Design: This study was a cross-sectional observational study. Subjects and Methods: The study population consisted of 127 patients aged 13–80 years admitted in wards of four different centers around Sawangi. The soft-tissue lesion and periodontal index were assessed using specialized design proforma. Results: None of the patients reported with healthy periodontal status. Oral lesions were observed in 48.03%, with most frequent sites of involvement were tongue, buccal mucosa, and soft palate. A majority of patients had generalized periodontitis. The prevalence of HIV-positive patient was 7.87%. The mean debris score was 1.35 ± 0.66 and mean calculus score was 1.21 ± 0.60 followed by pocket and clinical attachment level in the range of 1.77 ± 0.56 and 2.81 ± 0.71, respectively. Conclusions: The oral health professional should be made aware to treat the oral lesions in patients with TB with greater caution as the incidence of periodontal diseases and oral lesion, and HIV has become alarmingly higher with these classes of patients.
Platelets function as reservoirs for growth-factors and cytokines that are required for soft tiss... more Platelets function as reservoirs for growth-factors and cytokines that are required for soft tissue and bone regeneration, as discovered by Robert Marx in 1971. Choukroun's platelet research resulted in the development of a second-generation platelet concentrate (PRF) in 2001 [8]. The PRF clot forms a strong natural fibrin matrix that has a variety of growth-factors that aid tissue regeneration. The health risks linked with silica containing glass tubes used to collect blood for PRF are still a source of concern. T-PRF was developed to overcome the limitations of PRF [9].
Journal of Datta Meghe Institute of Medical Sciences University, 2018
Gingival enlargement is caused by gingival inflammation, fibrous overgrowth, or combination of bo... more Gingival enlargement is caused by gingival inflammation, fibrous overgrowth, or combination of both, leading to adverse consequences, such as difficulty in plaque control, mastication, altered speech, and esthetic and psychological problems. A 19-year-old male reported with the chief complaint of difficulty in mastication due to swelling of the gingiva for the past 6 months that bled profusely on slight provocation. The lesion gradually increased in size and similar lesions developed in other areas of the jaw, leading to unusual generalized massive gingival enlargement. Within a month of nonsurgical therapy which included supragingival and subgingival scaling, followed by oral hygiene instructions, enlargement reduced significantly. Complete resolution of the inflammatory gingival enlargement was observed after 3 months of nonsurgical therapy without any surgical intervention. Therefore, it will not be an exaggeration to say that scaling is still the gold standard treatment modality in the treatment of plaque-induced gingival enlargement.
Background: In this evolutionary era of dentistry, newer techniques have evolved for complete cov... more Background: In this evolutionary era of dentistry, newer techniques have evolved for complete coverage of multiple recession defects (MRDs). Since 2012, MRDs have been treated using the vestibular incision subperiosteal tunnel access (VISTA) approach and a variety of regenerating membranes with varying degrees of success. Platelet‑rich fibrin matrix (PRFM) membrane has shown a robust release of growth factors and was known to enhance the healing process. Thus, in this study we have evaluated the effectiveness of platelet-rich fibrin matrix (PRFM) with that of platelet-rich fibrin (PRF) membrane using the vestibular incision subperiosteal tunnel access (VISTA) technique in Miller Class I and II multiple gingival recession defects. Methods: This randomized, parallel designed, controlled, clinical study was conducted in 20 subjects (10 male and 10 female) having class I/II MGR defects either buccally or labially. The test group was treated with VISTA and PRFM membrane while the control...
Background Nanotechnology delivers a novel mode for restricting alveolar infections. The particle... more Background Nanotechnology delivers a novel mode for restricting alveolar infections. The particles intermingle through the bacterial cell wall improving antimicrobial action. Its amalgamation and glazing displayed greater germicidal properties. Arraying nanoparticles show sustained delivery of bactericidal property which ultimately aims the dentinal matrix. This study aimed to in vitro appraise the physical properties, antimicrobial, cytotoxicity, time-kill assay, anti-biofilm formation, and anti-inflammatory and antioxidant activity of Cu nanoparticle gel against periodontal pathogens. Materials & Methods Copper nanoparticles (procured from the Nano Research Laboratory, Jharkhand, India) gel was prepared and assessed for minimum inhibitory and minimum bactericidal concentration, MTT assay (cytotoxicity against periodontal and mouse fibroblasts), and time-kill assay at different time intervals against periodontal pathogens. Antibiofilm formation by the cell culture method, anti-infl...
Background: Amongst the specific plaque pathogen Aggregatibacter actinomycetemcomitans (Aa) ATCC ... more Background: Amongst the specific plaque pathogen Aggregatibacter actinomycetemcomitans (Aa) ATCC 43718 serotype b is one of the highly virulent bacteria that causes periodontitis. Probiotic therapy is a treatment in which the lactic acid bacteria in are utilized to impede the colonization and growth of the pathogenic bacteria to prevent the further formation of dental plaque. Objective: The present research aimed to evaluate inhibiting effect of purified bacteria from various commercially available yogurt product containing bacteria named (Lactobacillus casei strain Shirota; Lactobacillus bulgaricus and Streptococcus thermophilus; Lactobacillus reuteri Prodentis) on the growth of Aa. Methods: The research made use of the diffusion method by fixing Aa on BHIB (brain heart infusion broth) medium, incubated at 37°C and 24 hours later planted on MHA (Mueller-Hinton agar) media. Aa were divided into four subgroups each with a paper disk; group 1 consists of untreated bacteria (i.e., control group), group 2 with purified bacteria from Yakult 0.5 µL, group 3 with purified bacteria from Cimory Yogurt Drink 0.5 µL and group 4 with purified bacteria from BioGaia Prodentis 0.5 µL. All commercially available yogurt were treated to get the purified probiotic. Additionally, it was incubated for 24 hours at 37°C and later the inhibition zone diameter was observed. Results: In the research, it was found that the average impeding ability, so-called inhibition zone, in group 1 indicated 0 mm, group 2 indicated 12.70 mm, group 3 indicated 16.60 mm and group 4 indicated 19.60 mm. The statistical test outcomes showed a significance of 0.000 (p < 0.05). Conclusions: The purified bacteria from three probiotics indeed inhibit the growth of the Aa bacteria and a substantial difference in the diameter of the inhibition zone were found among the three probiotics.
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