Papers by Oluwatosin Adaramoye
Journal of basic and clinical physiology and pharmacology, 2010
Nigerian Journal of Pharmaceutical Research
Background: Diabetes mellitus is a metabolic disorder which leads to complications that affect th... more Background: Diabetes mellitus is a metabolic disorder which leads to complications that affect the heart, kidney, liver and other vital organs. Most reports on diabetes have focused on diabetes induction using male rat models in short term studies.Objective: Here, we described the induction of diabetes in female rat and the complications of diabetes following long term hyperglycemia in female Wistar rats.Method: Rats were assigned into two groups: control (n=10) and diabetic (n=10). The latter was administered a single (50mg/Kg body weight) intraperitoneal injection of streptozotocin. Bodyweight and blood glucose were assessed for 14 weeks, after which the animals were sacrificed and biochemical and morphology parameters of the liver, kidney and heart were determined.Results: Diabetic rats showed continuous emaciation post STZ induction. The glycated haemoglobin, protein level, lipid profile, liver and kidney function markers were significantly different (p<0.05) from the control...
Andrologia, 2020
In this study, Swiss male mice were intraperitoneally administered with titanium dioxide (TiO 2) ... more In this study, Swiss male mice were intraperitoneally administered with titanium dioxide (TiO 2) and zinc oxide (ZnO) nanoparticles (NPs) and their mixture (1:1) at doses between 9.38 and 75 mg/kg for 5 weeks to evaluate reproductive toxicity. Both NPs and their mixture significantly (p < .001) altered sperm motility, reduced sperm numbers and increased abnormalities, while their mixture induced more sperm abnormalities than either TiO 2 NPs or ZnO NPs. Both NPs and their mixture significantly (p < .05) reduced the LH level, while ZnO NPs alone and their mixture (p < .001) increased the testosterone levels at tested doses. The testes of exposed mice showed pathological changes and altered histomorphometrics. TiO 2 NPs and ZnO NPs individually induced a significant (p < .01) reduction in SOD and CAT activities, while the mixture significantly (p < .001) decreased CAT activity and increased SOD activity. TiO 2 NPs alone at 9.38 mg/kg induced a significant (p < .001) reduction in the GSH level, while both NPs and their mixture increased the MDA level significantly (p < .05). The data showed that the mixture had a synergistic interaction to induce testicular damage. Overall, oxidative stress may be involved in the NP-mediated testicular damage observed.
Polish Archives of Internal Medicine, 2009
IntroductIon Oxidative stress has been implicated in the etiology of several patho logies. objEct... more IntroductIon Oxidative stress has been implicated in the etiology of several patho logies. objEctIvEs The study was designed to investigate the levels of antioxidants and lipid peroxidation (LPO) in relation to prostate-specific antigen (PSA) levels in blood of Nigerian prostate cancer (PCa) patients. PAtIEnts And mEthods One hundred twenty PCa patients were assigned to 3 groups; group 1 (low grade) with a PSA level of 5-10 ng/ml (n = 33), group 2 (medium grade) with PSA of 11-20 ng/ml (n = 45) and group 3 (high grade) with PSA >20 ng/ml (n = 42). The co+ntrol group comprised 50 healthy subjects with PSA <3.0 ng/ml. rEsuLts Subjects with a PSA level of 11-20 ng/ml and PSA >20 ng/ml had significantly lower uric acid and reduced glutathione levels (p <0.05). A significant reduction (p <0.05) in plasma vitamin C and E levels was observed in these patients. The levels of vitamins C and E decreased by 27% and 77% in subjects with PSA >20 ng/ml, and by 25% and 47% in subjects with a PSA level of 11-20 ng/ml, respectively. Serum total bilirubin, alkaline phosphatase (ALP) and LPO were significantly (p <0.05) elevated in subjects with PSA >11 ng/ml. More specifically, total bilirubin, ALP and LPO levels were elevated by 75%, 66% and 107% in subjects with PSA at 11-20 ng/ml, and by 167%, 105%, 98% in subjects with PSA ≥20 ng/ml, respectively. Moreover, superoxide dismutase and catalase activities were lower (p <0.05) in all cancer patients. concLusIons The results confirmed the depletion of antioxidants in PCa patients, and an inverse relationship between antioxidants and PSA values in this group.
Journal of Ethnopharmacology, 2018
Root bark extract of Calliandra portoricensis (Jacq.) Benth. chemoprevents N-methyl-N-nitrosourea... more Root bark extract of Calliandra portoricensis (Jacq.) Benth. chemoprevents N-methyl-N-nitrosourea-induced mammary gland toxicity in rats,
Redox Report, 2017
Objectives: N-nitrosodimethylamine (NDMA) is known to elicit carcinogenic activity in the liver a... more Objectives: N-nitrosodimethylamine (NDMA) is known to elicit carcinogenic activity in the liver and kidney of animals. There is a dearth of information of its effect in testis. This study evaluated the protective role of betulinic acid (BA) against NDMA-induced redox imbalance in testes of rats. Methodology: Twenty-four male rats were assigned into four groups and treated with normal saline, BA, NDMA and [BA+NDMA]. BA (25 mg/kg) was given for 14 days, while NDMA (5 mg/kg) was given on days 7 and 12. Results: Administration of NDMA significantly increased the weight and relative weight of testes by 51 and 71%, respectively, while treatment with BA attenuated the weight-gain. Furthermore, NDMA decreased the sperm count, motility and live-dead ratio by 57, 36 and 37%, respectively, and increased total sperm abnormality by 56%. However, BA attenuated the changes in the spermiogram of NDMA-treated rats. NDMA significantly decreased the activities of antioxidative enzymes, follicle-stimulating and luteinizing hormones, while testicular levels of thiobarbituric acid reactive substances and total cholesterol were increased. Also, NDMA increased the activities of aniline hydroxylase and aminopyrine-N-demethylase. Supplementation with BA attenuated NDMAinduced alteration in these biochemical indices. Conclusion: BA protects against NDMA-induced redox imbalance via activation of antioxidative pathway.
Chemico-Biological Interactions, 2017
Prostate cancer (PCa) is an international health problem and search for its effective treatment i... more Prostate cancer (PCa) is an international health problem and search for its effective treatment is in progress. Punicalagin (PN), polyphenol from pomegranate fruit, is known to exhibit potent anticancer activity in lung, breast and cervical cells. However, there is paucity of information on its effect in PCa. This study evaluated anti-proliferative effects of PN and its effects on extrinsic pathway of apoptosis in PCa cells, and angiogenesis in chicken chorioallantoic membrane (CAM). Antioxidant activities of PN were determined by 2,2ediphenyl-1-picryhydrazyl (DPPH) radical scavenging and inhibition of lipid peroxidation (LPO) methods. PCa (PC-3 and LNCaP) and normal prostate (BPH-1) cells were cultured and treated with PN (10, 50 and 100 mM). Cytotoxicity and viability effects of PN were determined by lactate dehydrogenase (LDH) and XTT assays, respectively. Antiangiogenic effects were measured using CAM assay, while apoptosis was assessed by DNA fragmentation, enrichment factor by Cell Death Detection ELISA kit and expressions of caspases-3 and-8. Results showed that PN (10e200 mM) significantly scavenged DPPH and inhibited LPO in a concentration-dependent manner. Furthermore, PN (10e100 mM) concentration-dependently inhibited viability in PC-3 and LNCaP, while viability in BPH-1 was insignificantly affected. PN had low toxicity on cells in vitro at concentrations tested. Also, PN (100 mM) increased enrichment factor in PC-3 (2.34 ± 0.05) and LNCaP (2.31 ± 0.26) relative to control (1.00 ± 0.00). In addition, PN (50 mM) decreased the network of vessels in CAM, suggesting its antiangiogenic effect. Moreso, PN increased the expressions of caspases-3 and-8 in PC-3. Overall, PN exerts anti-proliferative activity in PCa cells via induction of apoptosis and anti-angiogenic effect.
Advance Journal of Food Science and Technology, 2015
Scent leaf (Ocimum gratissimum) enriched pepper-soup mix continues to remain an indigenous soup d... more Scent leaf (Ocimum gratissimum) enriched pepper-soup mix continues to remain an indigenous soup delicacy consumed for its nutritional and health benefits. There is however paucity of scientific data underlying its significance which could be further explored to advantage. In this study, dry soup mix was prepared from scent leaf (15%), ginger (30%), black pepper (25%), African nutmeg (17.5%) and scent pepper (12.5%) and evaluated for proximate, anti-oxidative and sensory properties using standard methods. The mix was reformulated to include 10% modified cocoyam (Colocasia esculenta) flour (thickener), 5% fresh and dried tea (Camellia sinensis) leaf following which it was re-characterized Concentration-dependent scavenging activity was observed for the entire samples with nitric oxide radical scavenging activity of scent leaf enriched soup (750 g/mL) exhibiting the highest value 77% compared to 57.7% for soup containing cocoyam. Total phenol, flavonoid contents and hydrogen peroxide radical scavenging activity differed in all the reformulated samples (p<0.05). A significant increase (>50%) in DPPH radical scavenging activity was observed as a result of tea leaf addition. The moisture, fat, crude fiber, ash, protein of the enhanced scent leaf enriched pepper-soup mix was 7.55, 28.4, 8.0, 6.2% and 11.2%, respectively. There was a significant difference (p<0.05) in the sensory qualities (color, aroma, taste, consistency and overall acceptability) between scent leaf and enhanced tea leaf pepper soup mix. The antioxidant potential, nutrient content and sensory attributes of this enhanced pepper soup mix holds great promise in further combating oxidative stress as in some disease conditions and rapid ageing.
West Indian Medical Journal, 2016
Objectives: The use of natural products derived from plants for therapeutic purpose is as ancient... more Objectives: The use of natural products derived from plants for therapeutic purpose is as ancient as human civilization. Sandbox tree, Hura crepitans L. (Euphorbiaceae) is one of such plant and has been reported to have many ethnomedicinal applications especially as antimicrobial, antiinflammatory and antihepatotoxic effects. This recent study was designed to determine the antihepatotoxic activity of the ethylacetate soluble fraction of the leaves and stem bark of H. crepitans and to isolate secondary metabolites. Methods: Chromatographic technique was used for isolation and Ultraviolet-Visible (UV), Infra-red (IR) and Nuclear Magnetic Resonance (NMR) spectroscopies were used for structural elucidation. Anti-hepatotoxicity study was carried out using carbon tetrachloride (CCl4) induced rat model and biochemical parameters: alanine aminotransferase (ALT), aspartate aminotransferase (AST), L-γglutamyltransferase (GGT), urea and creatinine (CREA) were assayed on the serum. Phytomicrographs of the liver samples were also taken and analyzed. Results: Our present study showed that biochemical studies of blood samples of CCl4 treated rats with value 105.0±0.001 AU in ALT showed significant increase in the level of serum enzyme activities reflecting liver injury but, 69.0±13.23 AU for leaves and 53.3±2.52 AU for bark (p<0.05) indicated protection of hepatic cells. AST, GGT, urea and CREA also reduced significantly. Daphnane diterpenes, daphnetoxin acid and huratoxin were isolated from H. crepitans in this recent study along with apocynin and methylpentadecanoate. Conclusion: H. crepitans significantly reduced the level of biochemical parameters indicating protection against hepatocellular injury. Isolates obtained from this plant could also serve as lead compounds in therapy of diseases involving hepatic injury.
The Tokai journal of experimental and clinical medicine, Jan 20, 2016
Tuberculosis (TB) is an infectious disease of international health priority. The combination of a... more Tuberculosis (TB) is an infectious disease of international health priority. The combination of anti-TB drugs (4-Tabs)- isoniazid (INH), rifampicin (RIF), pyrazinamide (PZA) and ethambutol (ETB) are effective in the management of the disease, however, their toxic effect is a major concern. The study was designed to evaluate the toxicity of anti-TB drugs in male Wistar rats and possible ameliorative effects of kolaviron (KV), a biflavonoid from Garcinia kola seeds. Twenty-eight rats were assigned into four groups; Group 1 (Control) received corn oil, Group 2 (4-Tabs) received therapeutic doses of INH (5 mg/kg), RIF (10 mg/kg), PZA (15 mg/kg) and ETB (15 mg/kg) in combination, Group 3 (4-Tabs + KV) received INH, RIF, PZA, ETB and KV (200 mg/kg) and Group 4 (KV) received KV (200 mg/kg) by oral gavage three times per week for 8 consecutive weeks. Administration of 4-Tabs caused oxidative stress resulting in significant (p = 0.031, 0.027) increase in malondialdehyde levels in the liver a...
Advances in Medical Education and Practice, 2016
recently revised its MBBS and BDS curricula to a competency-based medical education method of ins... more recently revised its MBBS and BDS curricula to a competency-based medical education method of instruction. This paper reports the process of revising the methods of instruction and assessment in the core basic medical sciences directed at producing medical and dental graduates with a sound knowledge of the subjects sufficient for medical and dental practice and for future postgraduate efforts in the field or related disciplines. The health needs of the community and views of stakeholders in the Ibadan medical and dental schools were determined, and the "old" curriculum was reviewed. This process was directed at identifying the strengths and weaknesses of the old curricula and the newer competences required for modern-day medical/dental practice. The admission criteria and processes and the learning methods of the students were also studied. At the end of the review, an integrated, system-based, community-oriented, person-centered, and competency-driven curriculum was produced and approved for implementation. Four sets of students have been admitted into the curriculum. There have been challenges to the implementation process, but these have been overcome by continuous faculty development and reorientation programs for the nonteaching staff and students. Two sets of students have crossed over to the clinical school, and the consensus among the clinical teachers is that their knowledge and application of the basic medical sciences are satisfactory. The Ibadan medical and dental schools are implementing their competency-based medical education curricula successfully. The modifications to the teaching and assessment of the core basic medical science subjects have resulted in improved learning and performance at the final examinations.
African Journal of Biotechnology, May 28, 2014
Antioxidants have been reported to prevent oxidative damage caused by free radicals and can be us... more Antioxidants have been reported to prevent oxidative damage caused by free radicals and can be used to ameliorate conditions in cardiovascular and inflammatory diseases. Ethnobotanical study revealed that the leaves of Lonchocarpus cyanescens are traditionally used in Africa to treat ulcer and arthritis. This study investigates the antioxidant activities of its extract and fractions. Acetone leaf extract of L. cyanescens was screened for 2,2-diphenylpicrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing power (FRAP), total phenol content and total flavonoid content using catechin as standard antioxidant. Bioguided column chromatographic separation was carried out and the resultant fractions were screened for antioxidant activities. Preliminary spectroscopic profile of the most active fraction was obtained. DPPH and FRAP methods showed that L. cyanescens had antioxidant activity which correlated with its phenolic and flavonoid contents. There was a higher correlation of the total phenol/flavonoid content to the antioxidant activity by the DPPH method [r2 = 0.9906, 9926 respectively] than the FRAP method [0.8635, 8840 respectively]. Bioactivity guided fractionation identified fraction F5 as the most active. 1 H and Infrared spectra indicated that the most active fraction contained flavonoids. Comprehensive in-vivo studies and toxicity profile of the extract will be required before considerations for development as a phyto-drug.
Journal of Basic and Clinical Physiology and Pharmacology, 2016
Tuberculosis (TB) is a global health problem. The effects of anti-TB drugs on male reproductive s... more Tuberculosis (TB) is a global health problem. The effects of anti-TB drugs on male reproductive system have not been properly evaluated. We investigated the effects of anti-TB drugs on testicular antioxidant indices, sperm characteristics and hormonal levels in rats, and the protective role of kolaviron (KV), a biflavonoid fromTwenty-eight male Wistar rats were assigned into four groups and orally treated with corn oil (control), anti-TB drugs [4-Tabs=isoniazid (5 mg/kg), rifampicin (10 mg/kg), pyrazinamide (15 mg/kg) and ethambutol (15 mg/kg) in combination], anti-TB drugs +KV and KV alone (200 mg/kg). Anti-TB drugs and KV were given three times per week for 8 weeks. In vitro, reducing power, inhibition of lipid peroxidation (LPO), diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical scavenging effects of KV were examined.KV at 10, 20, 50 and 100 μg/mL showed strong reducing potential and effectively scavenged DPPH and OH radicals in a concentration-dependent manner. Furthermore, ...
African Health Sciences, 2013
Background: Hypoglycaemic effect of kolaviron (KV), (biflavonoid from Garcinia kola) in streptozo... more Background: Hypoglycaemic effect of kolaviron (KV), (biflavonoid from Garcinia kola) in streptozotocin (STZ)-diabetic rats has been established. Objectives: To evaluate the possible protective effects of KV on cardiac, renal and hepatic tissues of STZ-diabetic rats. Methods: This study consists of four groups of 6 rats each. Groups one and two contained non-diabetic and untreateddiabetic rats, respectively. Groups three and four were made up of KV-and glibenclamide (GB)-treated diabetic rats, respectively. Results: STZ-intoxication caused a significant (p<0.05) increase in the relative weight of liver in diabetic rats. STZ-diabetic rats had significant increase (p<0.05) in the levels of fasting blood glucose (FBG), á-amylase and HbA 1c. A marked and significant (p<0.05) increase in the levels of cardiac, renal and liver marker indices such as serum creatine kinase, lactate dehydrogenase, creatinine, urea and alanine aminotransferase were observed in untreated diabetic rats. Also, untreated diabetic rats had significantly (p<0.05) elevated urinary glucose and protein and, lowered creatinine clearance. In KV-and GB-treated groups, the levels of FBG, á-amylase and HbA 1c were significantly (p<0.05) reduced, while treatment with KV significantly (p<0.05) attenuated the cardiac, renal and liver marker indices. Conclusion: KV offered significant antidiabetic and tissues protective effects in the rats.
African Journal of Biochemistry Research, 2015
Journal of integrative medicine, 2015
Prostate cancer (PCa) is a major health concern. Calliandra portoricensis (CP) is traditionally k... more Prostate cancer (PCa) is a major health concern. Calliandra portoricensis (CP) is traditionally known for its analgesic, anti-ulcerogenic and anticonvulsant properties. However, its antiproliferative properties for PCa still need to be investigated. Antioxidant activities of CP were determined by 1,1-diphenyl-2-picryhydrazyl (DPPH) and hydroxyl (OH(-)) radicals-scavenging methods. PC-3 and LNCaP (androgen-refractory and androgen-dependent PCa-derived cell lines) were cultured and treated with CP (10, 50 and 100 μg/mL). Effects of CP on cells were determined by cytotoxicity assay (lactate dehydrogenase, LDH) and viability assay (sodium 3'-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate, XTT). DNA fragmentation was detected by cell death detection enzyme-linked immunosorbent assay plus kit. CP was tested as an inhibitor of angiogenesis using chicken chorioallantoic membrane (CAM) assay. CP showed significant scavenging of DPPH and OH...
African health sciences, 2010
Prostate cancer is the most common type of cancer among men. To investigate the trace elements (S... more Prostate cancer is the most common type of cancer among men. To investigate the trace elements (Se, Zn, Cu and Cd) and vitamin E status of some Nigerian prostate cancer (PCa) patients relative to their prostate-specific antigen (PSA) values. Prostate cancer patients were assigned into groups 1, 2 and 3 with PSA of 5-10 ng/ml, 11-20 ng/ml and > 20 ng/ml, respectively. The results showed that the levels of whole blood superoxide dismutase (SOD) and serum Se and Zn were significantly lower (p< 0.05) in the PCa patients. Specifically, levels of SOD, Se and Zn decreased by 67%, 30% and 35%; 70%, 52% and 41%; 81%, 58% and 47%, in subjects with PSA of 5-10 ng/ml, 11-20 ng/ml and > 20 ng/ml, respectively. There were no significant differences (p> 0.05) in levels of Cu and Cd. Serum Cu/ Zn ratio were significantly higher in PCa patients. The Cu/ Zn ratios were 1: 1.2: 1.3 for subjects in groups 1, 2 and 3, respectively. Vitamin E levels in PCa patients were significantly lower an...
Asian Pacific Journal of Tropical Medicine, 2014
To investigate the antimalarial potential of kolaviron (KV), a biflavonoid fraction from Garcinia... more To investigate the antimalarial potential of kolaviron (KV), a biflavonoid fraction from Garcinia kola seeds, against Plasmodium berghei (P. berghei) infection in Swiss albino mice. Methods: The study consists of seven groups of ten mice each. Groups 栺, 栻and 栿 were normal mice that received corn oil, KV1 and chloroquine (CQ), respectively. Groups 桇 , 桋 , 桍 and 桏 were infected mice that received corn oil, CQ, KV1 and KV2, respectively. CQ, KV1 and KV2 were given at 10-, 100-and 200-mg/kg daily, respectively for three consecutive days. Results: Administration of KV1 and KV2 significantly (P<0.05) suppressed P. berghei-infection in the mice by 85% and 90%, respectively, while CQ produced 87% suppression relative to untreated infected group after the fifth day of treatment. Also, KV2 significantly (P<0.05) increased the mean survival time of the infected mice by 175%. The biflavonoid prevented a drastic reduction in PCV from day 4 of treatment, indicating its efficacy in ameliorating anaemia. Significant (P<0.05) oxidative stress assessed by the elevation of serum and hepatic malondialdehydewere observed in untreated P. berghei-infected mice. Specifically, serum and hepatic malondialdehyde levels increased by 93% and 78%, respectively in the untreated infected mice. Furthermore, antioxidant indices, viz; superoxide dismutase, catalase, glutathione-s-transferase, gluathione peroxidase and reduced gluathione decreased significantly (P<0.05) in the tissues of untreated P. berghei-infected mice. KV significantly (P<0.05) ameliorated the P. berghei-induced decrease in antioxidant status of the infected mice. Conclusions: This study shows that kolaviron, especially at 200 mg/kg, has high antimalarial activities in P. berghei-infected mice, in addition to its known antioxidant properties.
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Papers by Oluwatosin Adaramoye