Papers by Adaya D . Queen
Georgia Institute of Technology repository , 2022
Abstract
Inflammation is a regulated response to injury or infection that protects and repairs th... more Abstract
Inflammation is a regulated response to injury or infection that protects and repairs the body. When an inflammatory response is unregulated, hyper-inflammation can lead to organ damage and endothelium dysfunction. Recently, SARS-CoV-2 infections have been the cause of severe pneumonia, cardiovascular complications, and organ failures. Severe infections are accompanied by pulmonary hyper-inflammation and induced vascular endothelium damage. Protein nanoparticles can be modified to target and deliver anti-inflammatory cargo to the source of inflammation which is primarily among vascular endothelium cells. Specifically, this thesis explores the fabrication and optimization of protein nanoparticles with conjugated antibodies to specifically target endothelial cells. Protein nanoparticle targeting and uptake were confirmed in the cytoplasm of cultured Human Umbilical Vascular Endothelial Cells (HUVEC) using confocal microscopy and flow cytometry. Lastly, the biodistribution of these nanoparticles was assessed in healthy mice to understand the targeting of protein nanoparticles conjugated with targeting antibodies in vivo. Overall, this thesis demonstrates the impact that conjugated antibodies have on enhancing intracellular delivery and the biodistribution of these nanoparticles in vivo.
EURASIP Journal on Advances in Signal Processing
In a congested signal environment, it is difficult to obtain estimates of weak RF signal paramete... more In a congested signal environment, it is difficult to obtain estimates of weak RF signal parameters. Determining signal parameter estimates in real time is a challenge for electronic warfare receivers that aim to receive multiple simultaneous signals. Prior work provided estimates of weak signal parameters (weak signal frequency and weak signal amplitude) without taking into account any error introduced by analog-to-digital converters that are inherently part of digital signal processing systems. In order to obtain realistic estimates, we need to take error introduced by an ADC into account. The primary aim of this paper is to quantify error introduced by a single ideal ADC as a function of angle. This paper presents a method to estimate angle resolution and quantization levels in N-bit analog-to-digital converters (ADCs) for use in a weak radiofrequency (RF) simultaneous signal estimation process. The paper quantifies the error in the angle quantization of an N-bit ADC for an input...
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Papers by Adaya D . Queen
Inflammation is a regulated response to injury or infection that protects and repairs the body. When an inflammatory response is unregulated, hyper-inflammation can lead to organ damage and endothelium dysfunction. Recently, SARS-CoV-2 infections have been the cause of severe pneumonia, cardiovascular complications, and organ failures. Severe infections are accompanied by pulmonary hyper-inflammation and induced vascular endothelium damage. Protein nanoparticles can be modified to target and deliver anti-inflammatory cargo to the source of inflammation which is primarily among vascular endothelium cells. Specifically, this thesis explores the fabrication and optimization of protein nanoparticles with conjugated antibodies to specifically target endothelial cells. Protein nanoparticle targeting and uptake were confirmed in the cytoplasm of cultured Human Umbilical Vascular Endothelial Cells (HUVEC) using confocal microscopy and flow cytometry. Lastly, the biodistribution of these nanoparticles was assessed in healthy mice to understand the targeting of protein nanoparticles conjugated with targeting antibodies in vivo. Overall, this thesis demonstrates the impact that conjugated antibodies have on enhancing intracellular delivery and the biodistribution of these nanoparticles in vivo.
Inflammation is a regulated response to injury or infection that protects and repairs the body. When an inflammatory response is unregulated, hyper-inflammation can lead to organ damage and endothelium dysfunction. Recently, SARS-CoV-2 infections have been the cause of severe pneumonia, cardiovascular complications, and organ failures. Severe infections are accompanied by pulmonary hyper-inflammation and induced vascular endothelium damage. Protein nanoparticles can be modified to target and deliver anti-inflammatory cargo to the source of inflammation which is primarily among vascular endothelium cells. Specifically, this thesis explores the fabrication and optimization of protein nanoparticles with conjugated antibodies to specifically target endothelial cells. Protein nanoparticle targeting and uptake were confirmed in the cytoplasm of cultured Human Umbilical Vascular Endothelial Cells (HUVEC) using confocal microscopy and flow cytometry. Lastly, the biodistribution of these nanoparticles was assessed in healthy mice to understand the targeting of protein nanoparticles conjugated with targeting antibodies in vivo. Overall, this thesis demonstrates the impact that conjugated antibodies have on enhancing intracellular delivery and the biodistribution of these nanoparticles in vivo.