Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictal... more Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictaluri. Bakteri ini menyebabkan penyakit sistemik enteric septicemia of catfish (ESC). Penelitian bertujuan untuk mengetahui gejala klinis dan rerata waktu kematian akibat serangan bakteri E. ictalury pada ikan patin. Bakteri diinokulasi ke media BHIA (Brain Heart Infusion Agar, merek Conda Pronadisa) dan BHIB (Brain Heart Infusion Broth, merek Merck) diinkubasi pada suhu 24 oC selama 24 - 48 jam. Suspensi bakteri dengan kepadatan 2,7 x 101 – 1010 cfu/mL diinfeksikan pada 300 ekor ikan patin berukuran 11-12 cm dan diamati selama 7 hari. Penelitian menggunakan metode eksperimental dan Completely Randomized Design (CRD). Analisis lebih lanjut dengan Duncan multiple range test (DMRT). Hasil Penelitian menunjukkan gejala klinis yang muncul pada ikan patin antara lain, ikan berenang secara vertikal, warna tubuh pucat, nekrosis, petechiae, liver pucat dan membesar, ginjal berwarna gelap dan kaku...
Jeruju (Acanthus ilicifolius) is a Kalimantan tidal swamp plant that is potent to treat Aeromonas... more Jeruju (Acanthus ilicifolius) is a Kalimantan tidal swamp plant that is potent to treat Aeromonas hydrophila infection. This study aimed to determine the potential of Jeruju leaf extract as antioxidant and antibacterial agents to inhibit A. hydrophila growth. The research method included the sampling of Jeruju leaves in South Kalimantan, Indonesia. Then, the extracted sample was macerated with ethanol. The sample extracts were screened for phytochemical (Harborne) and metabolomic profiles (liquid chromatography and high-resolution mass spectrometry). The profile of compounds in the extract predicted biological activity using PASS server. Furthermore, the extracts were assayed for antibacterial activity (well diffusion and broth dilution), antioxidant activity 2,2-diphenyl-1-picrylhydrazyl (DPPH), and total phenol content (Folin-Ciocalteu). The phytochemical screening showed that the Jeruju leaves' ethanol extract contained alkaloids, flavonoids, tannins, phenolics, terpenoids, and steroids. The metabolomic profiling was dominated by betaine (41.61%) and choline (40.27%). The prediction of biological activity showed that the Jeruju leaf extract acted as a peptidoglycan glycosyltransferase enzyme inhibitor, DNA synthesis inhibitor, and free radical scavenger. The Jeruju leaf extract can inhibit A. hydrophila growth on glutamate starch phenol agar (9.09%). The ethanol extract of Jeruju leaves showed very strong antioxidant potential (IC 50 = 49.73 ± 1.14 µg/ml and 70.31% DPPH scavenging effect at 96 µg/ml), with a total phenol content of 32,667 ± 1,778.58 mg Gallic acid equivalent (GAE)/100 g dry extract. These research findings provide potential antioxidant and antibacterial activities for Jeruju (A. ilicifolius) leaves' ethanol extract for inhibiting A. hydrophila growth.
This study aims to determine the vaccine A.hydrophila ASB-01 is effective forthe control of MAS o... more This study aims to determine the vaccine A.hydrophila ASB-01 is effective forthe control of MAS on snakehead fish. Effectiveness of vaccination was evaluated through the antibody titer, survival, RPS (relative percent survival) and RWK (meantime of death). The study consisted of 5 treatments (immersion vaccination (R), oral(O), intramuscular injection (IM), intraperitoneal injection (IP) and control (PBS pH7.0) with 3 replications. Vaccination doses were 107 cells / ml. Booster vaccination after 14 days later. Dose is equal to the initial vaccination. Furthermore, the next day14 fish were challenged with A.hydrophila ASB-01. To obtain data on antibody titerblood draw done at the time before being vaccinated, shortly before the booster vaccination and 14 days after the booster vaccination. Challenged fish were observedfor 14 days to obtain data on survival, RPS and RWK. The result showed that allthese vaccines may increase the antibody titer, but the highest antibody titers obtained ...
Egyptian Journal of Aquatic Biology and Fisheries, 2020
Aeromonas hydrophila often attacks cultured catfish and causes motile aeromonad septicemia (MAS) ... more Aeromonas hydrophila often attacks cultured catfish and causes motile aeromonad septicemia (MAS) disease outbreak in South Kalimantan, Indonesia. Deaths from A.hydrophila attacks could reach 100% so that prevention needs to done through vaccination. This study aimed to examine the potential immunogenicity of 6 heat-killed A.hydrophila vaccine candidates, a strain of Banjar, South Kalimantan, Indonesia.
Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in ... more Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in cultivation of catfish (Pangasius hypophthalmus). MAS control can be done by administering vaccines. The purpose of this research is to get the right and effective dose of bivalent vaccine for controlling MAS at catfish. Both isolates will be used as vaccines are local strains of A. hydrophila CASO.01.G and SBMI.2. Both of them were isolated from disease catfish in the aquaculture ponds in Cindai Alus and Sungai Batang villages, Banjar Regency, South Kalimantan. Vaccines are made by heating (heat killed) at 100 o C for 60 minutes. A total of 13 test catfish measuring 9-13 cm were included in each aquarium. The design used was a completely randomized design with 5 treatments and 3 replications, namely bivalent vaccine with a dose of 10 9 cells/ml, 10 8 cells/ml, 10 7 cells/ml, 10 6 cells/ml, and control (PBS solution pH 7.0). The highest antibody titers in treatments vaccinated fish at a...
Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to... more Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish ( Pangasius hypophthalamus ). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150. The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvacci...
JSIPi (Jurnal Sains dan Inovasi Perikanan) (Journal of Fishery Science and Innovation), 2021
Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta aktivitas antibakteri sepuluh... more Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta aktivitas antibakteri sepuluh jenis tanaman terhadap bakteri Vibrio alginolyticus dan Aeromonas hydropilla. Dipilih 10 jenis tanaman yang sering digunakan sebagai obat dan banyak ditemukan di kota Kupang seperti tanaman Phyllanthus acidus, Euphorbia hirta, Persea gratissima, Euphorbia thymifolia, Morus australis, Justica gendarussa, Paederia scandeus, Annona squamosa, Sesbania sesban, dan Jatropha gossypifolia. Bagian tanaman yang digunakan adalah daun, kecuali E. hirta dan E. thymifolia digunakan seluruh bagian tanaman. Kesepuluh jenis tanaman tersebut dibuat bubuk, kemudian diekstraksi dengan cara dimaserasi secara bertingkat mulai dari pelarut n-heksan, etil asetat dan metanol, selain itu, juga digunakan ekstrak air tanaman tersebut. Semua jenis ekstrak tanaman diuji antibakteri dengan menggunakan metode cakram. Jenis ekstrak yang aktif selanjutnya diuji antibakteri pada konsentrasi 10%, 1% dan 0,1%. Has...
Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictal... more Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictaluri. Bakteri ini menyebabkan penyakit sistemik enteric septicemia of catfish (ESC). Penelitian bertujuan untuk mengetahui gejala klinis dan rerata waktu kematian akibat serangan bakteri E. ictalury pada ikan patin. Bakteri diinokulasi ke media BHIA (Brain Heart Infusion Agar, merek Conda Pronadisa) dan BHIB (Brain Heart Infusion Broth, merek Merck) diinkubasi pada suhu 24 oC selama 24 - 48 jam. Suspensi bakteri dengan kepadatan 2,7 x 101 – 1010 cfu/mL diinfeksikan pada 300 ekor ikan patin berukuran 11-12 cm dan diamati selama 7 hari. Penelitian menggunakan metode eksperimental dan Completely Randomized Design (CRD). Analisis lebih lanjut dengan Duncan multiple range test (DMRT). Hasil Penelitian menunjukkan gejala klinis yang muncul pada ikan patin antara lain, ikan berenang secara vertikal, warna tubuh pucat, nekrosis, petechiae, liver pucat dan membesar, ginjal berwarna gelap dan kaku...
Jeruju (Acanthus ilicifolius) is a Kalimantan tidal swamp plant that is potent to treat Aeromonas... more Jeruju (Acanthus ilicifolius) is a Kalimantan tidal swamp plant that is potent to treat Aeromonas hydrophila infection. This study aimed to determine the potential of Jeruju leaf extract as antioxidant and antibacterial agents to inhibit A. hydrophila growth. The research method included the sampling of Jeruju leaves in South Kalimantan, Indonesia. Then, the extracted sample was macerated with ethanol. The sample extracts were screened for phytochemical (Harborne) and metabolomic profiles (liquid chromatography and high-resolution mass spectrometry). The profile of compounds in the extract predicted biological activity using PASS server. Furthermore, the extracts were assayed for antibacterial activity (well diffusion and broth dilution), antioxidant activity 2,2-diphenyl-1-picrylhydrazyl (DPPH), and total phenol content (Folin-Ciocalteu). The phytochemical screening showed that the Jeruju leaves' ethanol extract contained alkaloids, flavonoids, tannins, phenolics, terpenoids, and steroids. The metabolomic profiling was dominated by betaine (41.61%) and choline (40.27%). The prediction of biological activity showed that the Jeruju leaf extract acted as a peptidoglycan glycosyltransferase enzyme inhibitor, DNA synthesis inhibitor, and free radical scavenger. The Jeruju leaf extract can inhibit A. hydrophila growth on glutamate starch phenol agar (9.09%). The ethanol extract of Jeruju leaves showed very strong antioxidant potential (IC 50 = 49.73 ± 1.14 µg/ml and 70.31% DPPH scavenging effect at 96 µg/ml), with a total phenol content of 32,667 ± 1,778.58 mg Gallic acid equivalent (GAE)/100 g dry extract. These research findings provide potential antioxidant and antibacterial activities for Jeruju (A. ilicifolius) leaves' ethanol extract for inhibiting A. hydrophila growth.
This study aims to determine the vaccine A.hydrophila ASB-01 is effective forthe control of MAS o... more This study aims to determine the vaccine A.hydrophila ASB-01 is effective forthe control of MAS on snakehead fish. Effectiveness of vaccination was evaluated through the antibody titer, survival, RPS (relative percent survival) and RWK (meantime of death). The study consisted of 5 treatments (immersion vaccination (R), oral(O), intramuscular injection (IM), intraperitoneal injection (IP) and control (PBS pH7.0) with 3 replications. Vaccination doses were 107 cells / ml. Booster vaccination after 14 days later. Dose is equal to the initial vaccination. Furthermore, the next day14 fish were challenged with A.hydrophila ASB-01. To obtain data on antibody titerblood draw done at the time before being vaccinated, shortly before the booster vaccination and 14 days after the booster vaccination. Challenged fish were observedfor 14 days to obtain data on survival, RPS and RWK. The result showed that allthese vaccines may increase the antibody titer, but the highest antibody titers obtained ...
Egyptian Journal of Aquatic Biology and Fisheries, 2020
Aeromonas hydrophila often attacks cultured catfish and causes motile aeromonad septicemia (MAS) ... more Aeromonas hydrophila often attacks cultured catfish and causes motile aeromonad septicemia (MAS) disease outbreak in South Kalimantan, Indonesia. Deaths from A.hydrophila attacks could reach 100% so that prevention needs to done through vaccination. This study aimed to examine the potential immunogenicity of 6 heat-killed A.hydrophila vaccine candidates, a strain of Banjar, South Kalimantan, Indonesia.
Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in ... more Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in cultivation of catfish (Pangasius hypophthalmus). MAS control can be done by administering vaccines. The purpose of this research is to get the right and effective dose of bivalent vaccine for controlling MAS at catfish. Both isolates will be used as vaccines are local strains of A. hydrophila CASO.01.G and SBMI.2. Both of them were isolated from disease catfish in the aquaculture ponds in Cindai Alus and Sungai Batang villages, Banjar Regency, South Kalimantan. Vaccines are made by heating (heat killed) at 100 o C for 60 minutes. A total of 13 test catfish measuring 9-13 cm were included in each aquarium. The design used was a completely randomized design with 5 treatments and 3 replications, namely bivalent vaccine with a dose of 10 9 cells/ml, 10 8 cells/ml, 10 7 cells/ml, 10 6 cells/ml, and control (PBS solution pH 7.0). The highest antibody titers in treatments vaccinated fish at a...
Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to... more Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish ( Pangasius hypophthalamus ). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150. The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvacci...
JSIPi (Jurnal Sains dan Inovasi Perikanan) (Journal of Fishery Science and Innovation), 2021
Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta aktivitas antibakteri sepuluh... more Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta aktivitas antibakteri sepuluh jenis tanaman terhadap bakteri Vibrio alginolyticus dan Aeromonas hydropilla. Dipilih 10 jenis tanaman yang sering digunakan sebagai obat dan banyak ditemukan di kota Kupang seperti tanaman Phyllanthus acidus, Euphorbia hirta, Persea gratissima, Euphorbia thymifolia, Morus australis, Justica gendarussa, Paederia scandeus, Annona squamosa, Sesbania sesban, dan Jatropha gossypifolia. Bagian tanaman yang digunakan adalah daun, kecuali E. hirta dan E. thymifolia digunakan seluruh bagian tanaman. Kesepuluh jenis tanaman tersebut dibuat bubuk, kemudian diekstraksi dengan cara dimaserasi secara bertingkat mulai dari pelarut n-heksan, etil asetat dan metanol, selain itu, juga digunakan ekstrak air tanaman tersebut. Semua jenis ekstrak tanaman diuji antibakteri dengan menggunakan metode cakram. Jenis ekstrak yang aktif selanjutnya diuji antibakteri pada konsentrasi 10%, 1% dan 0,1%. Has...
Uploads
Papers by Olga Olga