A White Fulani cow whose record reveals three-year old, weighing about 350kg belonging to DUFARMS... more A White Fulani cow whose record reveals three-year old, weighing about 350kg belonging to DUFARMS of the Federal University of Agriculture, Abeokuta was presented with a swelling on the left flank. Clinical evaluation of the animal revealed the following: rectal temperature, 38.2°C, heart rate, 60bpm and respiratory rate, 35bpm. A rectangular shaped mass, soft and warm to touch was palpated on the left flank. Pus was aspirated from the swelling with a sterile needle and syringe, and taken for biogram. Staphylococcus aureus was isolated from the aspirate which was sensitive to antibiotic, enrofloxacin, ciprofloxacin and gentamycin (Table 1). Total surgical extirpation of the encapsulated abscess was carried out aseptically under sedation and local anaesthesia and managed chemotherapeutically using antimicrobial, analgesic, anti-inflammatory agents post operatively.
Bulletin of animal health and production in Africa, 2017
There is a significant influx of cattle to Nigeria from neighbouring countries on daily basis alo... more There is a significant influx of cattle to Nigeria from neighbouring countries on daily basis along the transboundary areas without any form of veterinary inspection or quarantine. An assessment of protozoan parasite load in the ticks infesting cattle entering the country by hooves through a major trans-boundary route in Ogun State was carried out using Polymerase Chain Reaction and sequencing. The prevalence of Babesia bovis and B. bigemina in Boophilus ticks was 14/92 (15.2%) and 16/92 (17.4%) respectively. There was no detectable band for the Trypanosoma species and Ehrlichia ruminantium specific PCR. The prevalence of B. bovis, B. bigemina and Theileria annulata in Rhipicephalus ticks were 6/22 (27.3%), 4/22 (18.2%) and 4/22 (18.2%) respectively. The Amblyomma and Hyalomma ticks screened were not positive for any of the parasites. This is the first report on protozoan parasites detected in ticks infesting cattle entering Nigeria through a major trans-boundary route in Nigeria. The study shows that cattle entering Nigeria from Burkina Faso, Benin Republic, Niger Republic, Mali, Togo and Cote d’Ivoire are infested with adult ticks of various genera harbouring protozoan parasites that are pathogenic to cattle. Hence, we recommend that quarantine centres be established by government to screen and treat infected animals entering the country. Keywords : Cattle, Nigeria, PCR, Protozoans, Ticks, Trans-Boundary Route
Dermatophilosis caused by D. congolensis is a bacterial infection of the epidermis characterized ... more Dermatophilosis caused by D. congolensis is a bacterial infection of the epidermis characterized by thick scab formation on the integumentary of infected animals. Oftentimes, it is highly tasking to establish a confirmatory diagnosis of D. congolensis infection because of its close resemblance to other members of the family Actinomycetaceae some of which cause similar disease manifestations. The difficulty in establishing an accurate diagnosis may negatively impact treatment outcomes and thus the search for a more precise diagnostic approach becomes of great importance to limit the waiting time for therapeutic options. In this study, 164 scabs collected from clinically infected cattle in Abeokuta and Ilorin, Nigeria were examined using the direct microscopy, isolation and the polymerase chain reaction techniques. The scabs were examined microscopically after staining with Giemsa and also cultured using 5% sheep blood agar supplemented with 1000 units/ml of polymixin B sulphate. Antimicrobial susceptibility testing of the isolates was carried out using Mueller-Hinton agar supplemented with 5% sheep blood. Extracted DNA from isolates of the organism was used for the detection of a 16S rRNA gene using the polymerase chain reaction (PCR) technique. Out of the 164 samples examined in this study, 128 (78%) were positive by direct microscopy (DM) using Giemsa staining technique, while 54 (32.9%) were positive by isolation technique and 51 (31.1%) were confirmed with the PCR technique after bacterial isolation. Based on results from this study, we concluded that isolation technique is not only a recommendable option for the diagnosis of dermatophilosis but also offers an opportunity for antimicrobial susceptibility testing.
Recent reports raised concerns about the role that farm stock may play in the dissemination of ex... more Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids. E. coli isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried bla CTX-M-15 and bla TEM-1 , while K. pneumoniae subsp. pneumoniae isolates carried bla SHV-12 and bla TEM-1 . Conjugation experiments demonstrated that bla CTX-M-15 and bla TEM-1 were carried on a conjugative plasmid in E. coli , and PBRT identified this to be ...
A White Fulani cow whose record reveals three-year old, weighing about 350kg belonging to DUFARMS... more A White Fulani cow whose record reveals three-year old, weighing about 350kg belonging to DUFARMS of the Federal University of Agriculture, Abeokuta was presented with a swelling on the left flank. Clinical evaluation of the animal revealed the following: rectal temperature, 38.2°C, heart rate, 60bpm and respiratory rate, 35bpm. A rectangular shaped mass, soft and warm to touch was palpated on the left flank. Pus was aspirated from the swelling with a sterile needle and syringe, and taken for biogram. Staphylococcus aureus was isolated from the aspirate which was sensitive to antibiotic, enrofloxacin, ciprofloxacin and gentamycin (Table 1). Total surgical extirpation of the encapsulated abscess was carried out aseptically under sedation and local anaesthesia and managed chemotherapeutically using antimicrobial, analgesic, anti-inflammatory agents post operatively.
Bulletin of animal health and production in Africa, 2017
There is a significant influx of cattle to Nigeria from neighbouring countries on daily basis alo... more There is a significant influx of cattle to Nigeria from neighbouring countries on daily basis along the transboundary areas without any form of veterinary inspection or quarantine. An assessment of protozoan parasite load in the ticks infesting cattle entering the country by hooves through a major trans-boundary route in Ogun State was carried out using Polymerase Chain Reaction and sequencing. The prevalence of Babesia bovis and B. bigemina in Boophilus ticks was 14/92 (15.2%) and 16/92 (17.4%) respectively. There was no detectable band for the Trypanosoma species and Ehrlichia ruminantium specific PCR. The prevalence of B. bovis, B. bigemina and Theileria annulata in Rhipicephalus ticks were 6/22 (27.3%), 4/22 (18.2%) and 4/22 (18.2%) respectively. The Amblyomma and Hyalomma ticks screened were not positive for any of the parasites. This is the first report on protozoan parasites detected in ticks infesting cattle entering Nigeria through a major trans-boundary route in Nigeria. The study shows that cattle entering Nigeria from Burkina Faso, Benin Republic, Niger Republic, Mali, Togo and Cote d’Ivoire are infested with adult ticks of various genera harbouring protozoan parasites that are pathogenic to cattle. Hence, we recommend that quarantine centres be established by government to screen and treat infected animals entering the country. Keywords : Cattle, Nigeria, PCR, Protozoans, Ticks, Trans-Boundary Route
Dermatophilosis caused by D. congolensis is a bacterial infection of the epidermis characterized ... more Dermatophilosis caused by D. congolensis is a bacterial infection of the epidermis characterized by thick scab formation on the integumentary of infected animals. Oftentimes, it is highly tasking to establish a confirmatory diagnosis of D. congolensis infection because of its close resemblance to other members of the family Actinomycetaceae some of which cause similar disease manifestations. The difficulty in establishing an accurate diagnosis may negatively impact treatment outcomes and thus the search for a more precise diagnostic approach becomes of great importance to limit the waiting time for therapeutic options. In this study, 164 scabs collected from clinically infected cattle in Abeokuta and Ilorin, Nigeria were examined using the direct microscopy, isolation and the polymerase chain reaction techniques. The scabs were examined microscopically after staining with Giemsa and also cultured using 5% sheep blood agar supplemented with 1000 units/ml of polymixin B sulphate. Antimicrobial susceptibility testing of the isolates was carried out using Mueller-Hinton agar supplemented with 5% sheep blood. Extracted DNA from isolates of the organism was used for the detection of a 16S rRNA gene using the polymerase chain reaction (PCR) technique. Out of the 164 samples examined in this study, 128 (78%) were positive by direct microscopy (DM) using Giemsa staining technique, while 54 (32.9%) were positive by isolation technique and 51 (31.1%) were confirmed with the PCR technique after bacterial isolation. Based on results from this study, we concluded that isolation technique is not only a recommendable option for the diagnosis of dermatophilosis but also offers an opportunity for antimicrobial susceptibility testing.
Recent reports raised concerns about the role that farm stock may play in the dissemination of ex... more Recent reports raised concerns about the role that farm stock may play in the dissemination of extended-spectrum β-lactamase (ESBL)-producing bacteria. This study characterized the ESBLs in two Escherichia coli and three Klebsiella pneumoniae subsp. pneumoniae isolates from cases of clinical bovine mastitis in the United Kingdom. Bacterial culture and sensitivity testing of bovine mastitic milk samples identified Gram-negative cefpodoxime-resistant isolates, which were assessed for their ESBL phenotypes. Conjugation experiments and PCR-based replicon typing (PBRT) were used for characterization of transferable plasmids. E. coli isolates belonged to sequence type 88 (ST88; determined by multilocus sequence typing) and carried bla CTX-M-15 and bla TEM-1 , while K. pneumoniae subsp. pneumoniae isolates carried bla SHV-12 and bla TEM-1 . Conjugation experiments demonstrated that bla CTX-M-15 and bla TEM-1 were carried on a conjugative plasmid in E. coli , and PBRT identified this to be ...
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