Papers by Odair Correa Bueno
Genetics and Molecular Biology, 2022
In South America, Solenopsis saevissima and S. invicta are the most common fire ants. Nests are f... more In South America, Solenopsis saevissima and S. invicta are the most common fire ants. Nests are founded in areas under anthropic interference like urban or rural areas, but S. invicta is found preferentially in those with the greatest anthropic interference. However, we do not know the rates at which they exist in anthropized areas next to high density of native vegetation. Areas with 60 to 90% of native Atlantic Forest were selected to verify the occurrence of both species in rural and urban areas. We investigated the molecular diversity and applied the reconstruction of the ancestral state analysis for each species. A total of 186 nests were analyzed and we found that the two species had the same proportion in the urban area. However, S. saevissima had a higher rate of prevalence in the rural area, in addition to having a greater number of haplotypes and ancestry associated with this type of habitat for the region. S. invicta had the same number of haplotypes in both rural and urban regions, and less haplotypic diversity. We conclude that S. saevissima is a species typically associated with rural areas and S. invicta, although present, is not dominant in urban areas.
Biological Research, 2010
Linepithema humile Mayr is an ant species originally native to South America that has been spread... more Linepithema humile Mayr is an ant species originally native to South America that has been spread accidently throughout the globe through international trade. It is a serious urban and crop pest. Despite its economic importance, little is known about the larvae of this species apart from a brief description based on a few specimens. The present investigation is aimed at describing every immature stage of L. humile. Three larval instars were determined through the frequency distribution of the maximum width of head capsules from a sample of 525 larvae. The morphological descriptions were based on 150 eggs, 70 larvae, and 90 pupae examined by light and scanning electron microscopy. Some morphological characteristics reported to be typical of Linepithema Mayr larvae were confirmed-dolichoderoid body shape, presence of dorsal protuberance, sparse simple body hairs, presence of nine pairs of spiracles and dolichoderoid mandibles. We concluded that an earlier published description was based on queen larvae, and that the protuberance is only present in worker larvae. The information provided in this study may aid ant systematics and phylogenetics, as well provide a better understanding of the biology of this species.
Revista Caatinga
The number of studies on plant compounds with insecticidal activity has increased in recent years... more The number of studies on plant compounds with insecticidal activity has increased in recent years, and one of the primary targets of these compounds is leaf-cutter ants, which are considered the most important pests in Brazilian plantations, especially ants of the genus Atta. The objective of this study was to evaluate the toxic activity of the crude extract and fractions of the leaves of Esenbeckia pumila Pohl (Rutaceae) on Artemia salina and Atta sexdens rubropilosa and to perform a phytochemical study of this plant species. The toxicity of the extract and fractions was evaluated by determining the mean lethal concentration (LC50) on A. salina. The insecticidal activity was evaluated by feeding the ants an artificial diet containing the crude extract or fractions, and the results were analyzed using the log-rank test. The substances were isolated by chromatography, and the molecular structure was determined by spectroscopy. In the bioassay with A. salina, the ethanol extract and d...
Zootaxa
The ant Paratrechina longicornis is a cosmopolitan ant species that has been accidentally spread ... more The ant Paratrechina longicornis is a cosmopolitan ant species that has been accidentally spread by commerce around the world, and now is a pest of houses and hospitals. The morphology of its larval stages has been previously described without knowledge of the number of instars. The present study revisits the original description of P. longicornis larvae by adding differences observed among the three larval instars. Compared to other Paratrechina species studied so far, P. longicornis presents smaller larvae, less evident variation in mandible morphology, and clear variation in the morphology of head hairs between the analysed specimens of the second and third instar larvae. Ultrastructural images of the eggs, larval spiracle ornamentation, and pupae are also presented for the first time.
Papéis Avulsos de Zoologia
A new myrmecophilous silverfish (Zygentoma: Nicoletiidae: Subnicoletiinae) from Rio de Janeiro, B... more A new myrmecophilous silverfish (Zygentoma: Nicoletiidae: Subnicoletiinae) from Rio de Janeiro, Brazil, found living in fire ant (Solenopsis saevissima, Formicidae: Myrmicinae) nests is described: Allotrichotriura saevissima gen. nov. sp. nov. is compared with the other genera and subgenera known in the subfamily. The main diagnostic features would include the combination of body shape, body and head setation, morphology of praetarsus, and number of abdominal stylets and vesicles. Although further quests were attempted at the type-locality, only the original described material, exclusively composed of females, remains known.
Zeitschrift für Naturforschung B
The stems of Pilocarpus grandiflorus have afforded the new imidazole alkaloid 4,6-dehydro- 1,2,4,... more The stems of Pilocarpus grandiflorus have afforded the new imidazole alkaloid 4,6-dehydro- 1,2,4,5-tetrahydro-2,5-dioxopilocarpine in addition to the 17 known compounds germanicol, β - amiryn, ocotillone, stigmast-4-en-3-one, 3β -hydroxy-stigmast-5-en-7-one, 6β -hydroxy-stigmast-4- en-3-one, β -sitosterol, scopoletin, 3-(1’,1’-dimethylallyl)-scopoletin, elisin, dictamine, 4-methoxy-2- quinolone, platydesmine, syringaresinol, syringaldehyde, syringic acid and vanillic acid. Their structures were elucidated on the basis of chemical and spectroscopic evidence. The phenolic compounds vanillic acid and syringaldehyde and the furoquinoline alkaloid platydesmine exhibited antifungal activity against Leucoagaricus gongylophorus, the symbiotic fungus of leaf-cutting ants (Atta sexdens rubropilosa).
Current Microbiology
Bacterial endosymbionts are common in all insects, and symbiosis has played an integral role in a... more Bacterial endosymbionts are common in all insects, and symbiosis has played an integral role in ant evolution. Atta sexdens rubropilosa leaf-cutting ants cultivate their symbiotic fungus using fresh leaves. They need to defend themselves and their brood against diseases, but they also need to defend their obligate fungus gardens, their primary food source, from infection, parasitism, and usurpation by competitors. This study aimed to characterize the microbial communities in whole workers and different tissues of A. sexdens rubropilosa queens using Ion Torrent NGS. Our results showed that the microbial community in the midgut differs in abundance and diversity from the communities in the postpharyngeal gland of the queen and in whole workers. The main microbial orders in whole workers were Lactobacillales, Clostridiales, Enterobacteriales, Actinomycetales, Burkholderiales, and Bacillales. In the tissues of the queens, the main orders were Burkholderiales, Clostridiales, Syntrophobacterales, Lactobacillales, Bacillales, and Actinomycetales (midgut) and Entomoplasmatales, unclassified c-proteobacteria, and Actinomycetales (postpharyngeal glands). The high abundance of Entomoplasmatales in the postpharyngeal glands (77%) of the queens was an unprecedented finding. We discuss the role of microbial communities in different tissues and castes. Bacteria are likely to play a role in nutrition and immune defense as well as helping antimicrobial defense in this ant species.
Chemosphere, 2016
h i g h l i g h t s The genotoxic potential of two new metallic-insecticides was evaluated. Only ... more h i g h l i g h t s The genotoxic potential of two new metallic-insecticides was evaluated. Only one of them induced genotoxic alterations in Allium cepa. The genotoxicity can be related to one of the components of its formula. None of them was genotoxic to Tradescantia pallida. One of the metallic-insecticide tested is environmentally safer.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, Jan 20, 2016
Liver is very sensitive to environmental contaminants such as pesticides, it being the first targ... more Liver is very sensitive to environmental contaminants such as pesticides, it being the first target of toxicity of a substance. The objective of this study was to investigate the possible effects of the insecticide imidacloprid (IMI) on the liver of Oreochromis niloticus according concentrations used for growing sugarcane. A semi-quantitative analysis of histopathological alterations of IMI on liver was performed by light microscopy and cellular labeling of heat shock proteins (HSP70) by immunohistochemistry. The most common changes in liver at all concentrations of IMI were hydropic degeneration, pyknotic nuclei, and loss of cell limits. Steatosis and increased levels of HSP70 were detected in hepatocytes with the highest concentration of IMI. In conclusion, the tested concentrations of IMI induced histopathological changes in the liver of O. niloticus and active defence mechanisms to maintain the morphophysiological integrity of the liver. This insecticide has a toxicity potential...
Pest management science, Jan 19, 2016
Leaf-cutter ants are considered a major herbivore and agricultural pest in the Neotropics. They a... more Leaf-cutter ants are considered a major herbivore and agricultural pest in the Neotropics. They are often controlled by environmentally persistent insecticides. Biological control using pathogenic fungi is regarded as an alternative for the management of these insects. Here, we assess whether the filamentous fungus Syncephalastrum sp. is a pathogenic microorganism, responsible for a characteristic disease in fungus gardens. We also characterize the damage caused by this fungus by evaluating physiological and behavioral responses of Atta sexdens rubropilosa sub-colonies infected with Syncephalastrum sp. Syncephalastrum sp. fulfills Koch's postulates, characterizing it as a pathogenic microorganism. Ant workers recognize the infection and remove contaminated fragments from the fungus garden. Syncephalastrum sp. infection causes an interruption of foraging activity, an increase in ant mortality, sub-colony deterioration and increase in the amount of waste generated, all resulting i...
Journal of Apicultural Research, 2003
... BUENO ; Résumé / Abstract. Two species of plants commonly known as barbatimao occur in Brazil... more ... BUENO ; Résumé / Abstract. Two species of plants commonly known as barbatimao occur in Brazil, Stryphnodendron adstringens (Fabaceae) (true barbatimão) andDimorphandra mollis (Caesalpiniaceae) (false barbatimao). ...
Pest Manag Sci, 2004
The focus of this study was the identification of compounds from plant extracts for use in crop p... more The focus of this study was the identification of compounds from plant extracts for use in crop protection. This paper reports on the toxic activity of fractions of leaf extracts of Ricinus communis L (Euphorbiaceae) and isolated active compounds in the leaf-cutting ant Atta sexdens rubropilosa Forel and its symbiotic fungus Leucoagaricus gongylophorus (Singer) Möller. The main compounds responsible for activity against the fungus and ant in leaf extracts of R communis were found to be fatty acids for the former and ricinine for the ants.
Microsc Res Technique, 2010
Ants present a pair of metapleural glands located at the posterolateral end of the thorax. Becaus... more Ants present a pair of metapleural glands located at the posterolateral end of the thorax. Because of its importance in the social organization of ants, the present study was aimed at describing the morphophysiology of this gland in three worker castes of Acromyrmex coronatus, focused on secretory activity using histological and histochemical techniques. Our findings revealed that the secretory and the storage portions of this gland are connected by extracytoplasmic portion of canaliculi that drain the secretion from each secretory cell to the collecting chamber. This secretion contains glycoproteins. In minor workers, the secretion contains higher levels of polysaccharides when compared to that of major workers, supporting the role of the metapleural gland in the maintenance of the fungus garden. The nucleus as well as cytoplasm of secretory cells were strongly positive for RNA indicating that these cells are active in the synthesis of proteins and lipids, compounds found in the final secretion. The variant of the CEC revealed that the secretory activity of the entire gland is synchronous, as all cells exhibit the result.
Fire ants are well-known by their aggressive stinging behavior, causing many stinging incidents o... more Fire ants are well-known by their aggressive stinging behavior, causing many stinging incidents of medical importance. The limited availability of fire ant venom for scientific and clinical uses has restricted, up to now, the knowledge about the biochemistry, immunology, and pharmacology of these venoms. For this study, S. invicta venom was obtained commercially and used for proteomic characterization. For this purpose, the combination of gel-based and gel-free proteomic strategies was used to assign the proteomic profile of the venom from the fire ant S. invicta. This experimental approach permitted the identification of 46 proteins, which were organized into four different groups according to their potential role in fire ant venom: true venom components, housekeeping proteins, body muscle proteins, and proteins involved in chemical communication. The active venom components that may not present toxic roles were classified into three subgroups according to their potential functions: self-venom protection, colony asepsis, and chemical communication. Meanwhile, the proteins classified as true toxins, based on their functions after being injected into the victims' bodies by the fire ants, were classified in five other subgroups: proteins influencing the homeostasis of the victims, neurotoxins, proteins that promote venom diffusion, proteins that cause tissue damage/inflammation, and allergens.
Sociobiology, 2007
... Maia, BHLNS, JR de Paula, J. Sant'Ana, MFGF da Silva, JB Fernandes, PC Vieira, M... more ... Maia, BHLNS, JR de Paula, J. Sant'Ana, MFGF da Silva, JB Fernandes, PC Vieira, MSS Costa, OS Ohashi, & JNM Silva 2000. ... Rodríguez-Gamboa, T., JB Fernandes, E. Rodrigues, MFGF da Silva, PC Vieira, CM Barrios, O. Castro-Castillo, SR Victor, FC Pagnocca, OC Bueno & ...
Genetica, Jan 25, 2016
The great diversity of Camponotus, high levels of geographic, intraspecific and morphological var... more The great diversity of Camponotus, high levels of geographic, intraspecific and morphological variation common to most species of this genus make the determination of the interspecific limits of Camponotus a complex task. The Cytochrome c oxidase 1 (COI) gene was sequenced in this study to serve as an auxiliary tool in the identification of two taxa of Camponotus thought to be morphologically similar. Additionally, characteristics related to nesting were described. Five to fifteen workers from twenty-one colonies were analyzed, collected from twigs scattered in the leaf litter and from trees located in different regions of Brazil. Phylogenetic reconstructions, haplotype network, and nesting strategies confirmed the existence of two species and that they correspond to Camponotus senex and Camponotus textor. Our results emphasize that the COI can be used as an additional tool for the identification of morphologically similar Camponotus species.
Insectes Soc, 1982
... D, BEIG (1), O. CORREA BUENO (1) R. ALVES da CUNHA (2) H. JORGE de MORAES (1) ... Because the... more ... D, BEIG (1), O. CORREA BUENO (1) R. ALVES da CUNHA (2) H. JORGE de MORAES (1) ... Because these additional eggs produced adult males, they were probably laid by nurse bees ('workers) after the queen had oviposited in the cells concerned. ...
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Papers by Odair Correa Bueno