Papers by Niels Hendriksen
genetically modified carnation Moonaqua 123.8.12 with a modified colour, for import of cut flower... more genetically modified carnation Moonaqua 123.8.12 with a modified colour, for import of cut flowers for ornamental use, under Part C of Directive
Request from the European Commission related to the safeguard clause
application (Reference EFSA-GMO-UK-2005-20) for the placing on the market of the insect-resistant... more application (Reference EFSA-GMO-UK-2005-20) for the placing on the market of the insect-resistant and herbicide-tolerant genetically modified maize 59122 x NK603, for food and feed uses, and import and processing
SCIENTIFIC OPINION Applications (EFSA-GMO-RX-MON810) for renewal of authorisation for the continu... more SCIENTIFIC OPINION Applications (EFSA-GMO-RX-MON810) for renewal of authorisation for the continued marketing of (1) existing food and food ingredients produced from genetically modified insect resistant maize MON810; (2) feed consisting of and/or containing maize MON810, including the use of seed for cultivation; and of (3) food and feed additives, and feed materials produced from maize MON810, all under Regulation (EC) No 1829/2003
Hungary submitted to the European Commission additional information regarding the cultivation of ... more Hungary submitted to the European Commission additional information regarding the cultivation of genetically modified maize MON810 to support a safeguard measure initially notified, under Article 23 of Directive 2001/18/EC, by the Hungarian authorities on 20 January 2005 to provisionally prohibit the use and sale of the authorised genetically modified maize MON810 on its territory. The European Commission received from Hungary a written submission made of four supporting documents. As a consequence, the European Commission requested in a letter, dated 18 April 2008, the EFSA’s Scientific Panel on Genetically Modified Organisms (GMO Panel) to assess whether the information submitted by Hungary comprises information affecting the environmental risk assessment of existing information on the basis of new scientific knowledge such that detailed grounds exist to consider that the above authorised GMO, for the uses laid down in the corresponding consent, constitute a risk to the environmen...
concerning the extension of validity and amendment of an existing safeguard measure
SCIENTIFIC OPINION Application (Reference EFSA-GMO-NL-2007-37) for the placing on the market of t... more SCIENTIFIC OPINION Application (Reference EFSA-GMO-NL-2007-37) for the placing on the market of the insect-resistant genetically modified maize MON89034, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto1
SUMMARY This document provides an opinion of the Scientific Panel on Genetically Modified Organis... more SUMMARY This document provides an opinion of the Scientific Panel on Genetically Modified Organisms (GMO Panel) of the European Food Safety Authority (EFSA) on genetically modified maize MON89034 (Unique Identifier MON-89Ø34-3) developed to provide resistance to certain insect pests. In delivering its scientific opinion, the GMO Panel considered the new application EFSA- GMO-NL-2007-37, additional information provided by the applicant (Monsanto)
Journal of Applied Ecology, 2011
affected by weed-management regimes. We discuss the relevance for management of maize events wher... more affected by weed-management regimes. We discuss the relevance for management of maize events where cry1F is combined (stacked) with a herbicide-tolerance trait. This exemplifies how interactions between biota may occur when different traits are stacked irrespective of interactions between the proteins themselves and highlights the importance of accounting for crop management in the assessment of the ecological impact of GM plants.
Biology and Fertility of Soils, 1997
Bacteria producing secondary metabolites with antagonistic effects on fungal pathogens have recei... more Bacteria producing secondary metabolites with antagonistic effects on fungal pathogens have received attention during the last decades as an alternative to chemical pesticides. They, however, might also have effects on indigenous soil organisms like bacteria and protozoa, the latter ones being among the most important grazers of bacteria in soil. The present study reports on the effect of the potential biocontrol agent Pseudomonas fluorescens CHA0 and its genetically modified derivative CHA0/pME3424 on indigenous soil bacteria and protozoa in a soil system. CHA0/pME3424 overproduces two of the secondary metabolites produced by CHA0: the polyketide antibiotics pyoluteorin (Plt) and 2,4-diacetylphloroglucinol (DAPG). P. fluorescens CHA0/gfp1 and CHA0/pME3424 both negatively affected the abundance of soil bacteria and protozoa and the genetic community structure of Kinetoplastida studied by PCR-DGGE. The negative effects were detectable after 14 days but were decreasing and are expected to be temporary. The overproducer of secondary metabolites did not differ in effect from the wild type. The soil respiration and bacterial genetic community structure were not significantly affected. The study shows the soil bacteria and protozoa to be temporary affected by bacteria producing secondary metabolites, which can have implications for nutrient-cycling in soil and environmental risks of biocontrol agents.
Applied and Environmental Microbiology, 2015
Bacillus thuringiensisserovar israelensis is a wide-spread soil bacterium affiliated with theB. c... more Bacillus thuringiensisserovar israelensis is a wide-spread soil bacterium affiliated with theB. cereusgroup (Bcg) and is widely used in biocontrol products applied against mosquito and black fly larvae. For monitoring and quantification of appliedB. thuringiensisserovar israelensis and its effect on indigenousB. thuringiensisserovar israelensis and Bcg assemblages, efficient and reliable tools are essential. The abundance and properties ofB. thuringiensisserovar israelensis strains in the environment traditionally have been investigated with cultivation-dependent techniques, which are hampered by low sensitivity and the morphological similarity betweenB. cereusandB. thuringiensis. Currently available PCR-based detection and quantification tools target markers located on plasmids. In this study, a new cultivation-independent PCR-based method for efficient and specific quantification ofB. thuringiensisserovar israelensis and Bcg is presented, utilizing two sets of PCR primers targetin...
Folia Microbiologica, 2011
The aim of this study was to assess the diagnostic properties of the two selective plating media ... more The aim of this study was to assess the diagnostic properties of the two selective plating media and a chromogenic medium for identification of Bacillus cereus. The 324 isolates were B. cereus (37%), Bacillus weihenstephanensis (45%) or Bacillus thuringiensis (18%), as identified by a new combination of techniques. All isolates were growing on mannitol-egg yolk-polymyxin agar (MYP), and they did not form acid from mannitol. However, a significant lower number of B. thuringiensis isolates did not show lecithinase activity. All isolates were also growing on polymyxin-egg yolk-mannitol-bromothymol blue agar (PEMBA); however, 11% isolates indicated that they did produce acid from mannitol, and 15% isolates did not show any lecithinase activity. Five of the isolates did not grow at all on the chromogenic agar, and 14 of the growing isolates were β-glucosidase negative. It is concluded that the two recommended selective plating media MYP and PEMBA for detection of B. cereus group bacteria both have their limitations for identification of some B. cereus, B. weihenstephanensis or B. thuringiensis. However, MYP is preferable compared to PEMBA. The chromogenic medium has its own advantages and limitations, and some of the limitations seem to be solved by incubation at 30°C instead of the recommended 37°C.
FEMS Microbiology Letters, 2006
The objectives of the study were to develop a specific procedure for quantification and identific... more The objectives of the study were to develop a specific procedure for quantification and identification of Bacillus thuringiensis kurstaki HD1, which is used as a biopesticide, and to quantify its presence in different kinds of cabbage for human consumption. We found that B. thuringiensis kurstaki HD1 can be distinguished from other B. thuringiensis strains by its unique random amplification of polymophic DNA-PCR pattern with the OPA9 primer and the presence of the flagellin genes, as detected by the primers FLAB1 and FLAB2. We detected from one to 100 Bacillus cereus-like bacteria in 10 batches of five different cabbage products for consumption. As many as 73 out of 134 isolates (53.7%) were identical with B. thuringiensis kurstaki HD1. The results show that B. thuringiensis kurstaki HD1 from biopesticides can be found in vegetables for human consumption.
Ecography, 1988
Respiratory loss and bulk transport of organic matter from dung pats in a Danish pasture were mea... more Respiratory loss and bulk transport of organic matter from dung pats in a Danish pasture were measured by a chromic oxide marker technique. Measurements covered the period until a dung age of 7-8 weeks. On average, respiration and transport contributed 46% and 54% of organic matter disappearance in three spring experiments: in three autumn experiments the corresponding values were 25% and 75%. Thus, both processes are important. The rate of transport was highest at the bottom of the pat, whereas the highest respiratory rate occurred in the top and periphery. During the first 10–14 days transport predominated, probably owing to the activity of dung insects, whereas respiration was remarkably low. During the remaining period, transport was probably affected mainly by earthworms. Respiration seemed to be mostly microbial and to proceed during dung pat decay in essentially the same way as did cellulolytic activity. Earthworms did not significantly affect the respiration of pats.
Bacteriophage therapy and application of phages as biocontrol in plant production and food proces... more Bacteriophage therapy and application of phages as biocontrol in plant production and food processing, all necessitates acquisition of suitable phages. Depending on purpose, the selection criteria of phage characteristics include lifestyle (lytic/lysogenic), host range, physical stability and absence of unwanted genetic traits such as integrases, antibiotic resistance or bacterial virulence factors. The exclusivity of antibiotic resistant clinical infections and possible development of phage-resistance instigates a need to continually build sizeable phage libraries and also be able to rapidly isolate and characterise novel phages of specified bacterial hosts. Current methods for phage isolation are both laborious and time consuming, suitable only for the isolation of a limited number of phages. Thus, we developed the High-ThroughputScreening (HITS) method for phages for fast isolation and identification of potentially hundreds of distinct phages against single hosts. This scalable m...
Environmental pollution (Barking, Essex : 1987), 2018
Recently, concerns have been raised that residues of glyphosate-based herbicides may interfere wi... more Recently, concerns have been raised that residues of glyphosate-based herbicides may interfere with the homeostasis of the intestinal bacterial community and thereby affect the health of humans or animals. The biochemical pathway for aromatic amino acid synthesis (Shikimate pathway), which is specifically inhibited by glyphosate, is shared by plants and numerous bacterial species. Several in vitro studies have shown that various groups of intestinal bacteria may be differently affected by glyphosate. Here, we present results from an animal exposure trial combining deep 16S rRNA gene sequencing of the bacterial community with liquid chromatography mass spectrometry (LC-MS) based metabolic profiling of aromatic amino acids and their downstream metabolites. We found that glyphosate as well as the commercial formulation Glyfonova450 PLUS administered at up to fifty times the established European Acceptable Daily Intake (ADI = 0.5 mg/kg body weight) had very limited effects on bacterial ...
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Papers by Niels Hendriksen