Papers by Nazario Cappello
Annals of Oncology, 1998
Background: Sezary syndrome (SS) prognostic factors are not well defined because of the rarity of... more Background: Sezary syndrome (SS) prognostic factors are not well defined because of the rarity of this disease. The specific goal of this prospective study was to assess by multivariate analysis the predictive value with respect to survival of a series of clinical, haematological and immunological parameters taken at SS diagnosis. Patients and methods: A cohort of 62 SS patients diagnosed and followed since 1975 was examined, and 51 were included in the multivariate analysis model. Results: The median survival time was 31 months (range: 1 month-15.7+ years), and the five-year survival rate 33.5%. The following variables were found by univariate analysis to be associated with a poor prognosis at the time of SS diagnosis: previous history of mycosis fungoides (P = 0.013), high number of circulating leukocytes {P = 0.001), Sezary cells (SC) (P < 0.001) and CD4+ cells (P < 0.001), presence of large circulating SC (P < 0.001), above normal range LDH serum levels (P = 0.015), presence of PAS-positive inclusions in the cytoplasm of circulating SC (P < 0.001), high CD4/CD8 ratio (P = 0.004) and a CD7 negative circulating SC phenotype (P < 0.001). Among them, the stepwise multivariate analysis selected as adverse independent prognostic factors: PAS-positive cytoplasmic inclusions (P = 0.001), CD7 negative phenotype (P = 0.018) and presence of large circulating SC (P = 0.045). Conclusions: Two low-/high-risk groups have been singled out on the basis of the risk index. Patients with no or one adverse prognostic feature(s) (risk index ^ 1; n-31) share a slow disease course and a relatively favorable prognosis (fiveyear survival: 58%); on the other hand, patients with 2 or 3 adverse prognostic features (risk index > 1; n = 20) are characterized by an aggressive disease course not modifiable by traditional therapies (five-year survival: 5%).
Rev Col Bras Cir, Dec 1, 1993
Tendo em vista a escassa literatura a respeito de correlacao entre a histometria dos carcinomas d... more Tendo em vista a escassa literatura a respeito de correlacao entre a histometria dos carcinomas de tireoide e o prognostico desses tumores, objetivou-se, nesta pesquisa, correlacionar parâmetros histometricos do carcinoma folicular da tireoide com o tempo de sobrevida apos tratamento. Em carcinomas foliculares da tireoide de 60 pacientes foram realizadas medicoes no nucleo das celulas malignas (area, perimetro, diâmetro maximo, diâmetro minimo) e calculados indices (razao dos diâmetros, fator de forma e indice de atividade mitotica). Esses parâmetros foram correlacionados com o tempo de sobrevida. Por outro lado, parâmetros clinicos e patologicos (sexo, idade, extensao local, comprometimento linfonodal, grau de diferenciacao celular) tambem foram correlacionados com o prognostico. Foram realizados calculos autuariais e analise estatistica uni e multivariada. Constatou-se que a sobrevida foi significantemente influenciada por variaveis clinicas e patologicas, tais como a idade dos pacientes e a extensao loco-regional do tumor. Nenhuma correlacao estatisticamente significante pode ser demonstrada entre parâmetros morfometricos e sobrevida, apesar dos resultados sugerirem associacao entre o tamanho do nucleo das celulas malignas e a agressividade do tumor(AU)
Digestive Diseases and Sciences, 1999
Assessment of hepatic function is based on bothliver blood tests and functional tests, the extens... more Assessment of hepatic function is based on bothliver blood tests and functional tests, the extensiveapplication of which is still controversial. The aim ofthis study was to evaluate the clinical utility of a few selected tests as discriminatory andprognostic indexes: serum albumin, pseudocholinesterase,prothrombin time, as well as galactose eliminationcapacity and hepatic sorbitol clearance. Two separate studies were performed: Study I to
Annali dell'Ospedale Maria Vittoria di Torino
A socio-analytic investigation, showing mental images of health personnel and their influence on ... more A socio-analytic investigation, showing mental images of health personnel and their influence on the course of pregnancy, child birth and child nursing has been performed at the Obstetrics and Neonatology Departments of Maria Vittoria Hospital, Torino, Italy. Through a statistical analysis on the mothers population during one year (1986 Nov-1987 Nov) a significant reduction of maternal delivery stress and neonatal risk has been found in relation to the "participation to the psycho-prophylactic courses" and to the "presence of fathers during delivery". Answers of mothers to a questionnaire are reported. This work points out the importance of a child birth's humanization.
Minerva medica, Jan 4, 1963
Annali italiani di chirurgia
Histological material from 60 cases of follicular carcinoma of the thyroid operated between 1962 ... more Histological material from 60 cases of follicular carcinoma of the thyroid operated between 1962 and 1987 was examined morphometrically to see whether this would produce data that could be correlated with survival. Nuclear parameters (area, perimeter, minimum and maximum diameter and their ratio, form factor) and the mitotic index were investigated. At the same time, account was taken of certain clinical parameters (sex, age, degree of differentiation, size of the primary lesion, extension of the tumour) potentially correlated with prognosis. Survival in relation to all these variables was examined by means of an actuarial method and subjected to both uni- and multivariate analysis. The results, although suggesting the existence of a relationship between nuclear size and the aggressiveness of this type of tumour, failed to demonstrate a significant correlation between these morphometric parameters and survival after treatment. By contrast, survival was significantly influenced by so...
American journal of human genetics, 1989
The genetic structure of the population of Ferrara Province in the Po delta in Italy was investig... more The genetic structure of the population of Ferrara Province in the Po delta in Italy was investigated using chi 2 analysis, kinship analysis, analysis of correspondences, and geographical mapping of principal components of gene frequencies. chi 2 Analysis tests for Hardy-Weinberg equilibrium and for heterogeneity of gene and phenotype frequencies; kinship analysis tests for association between indicators of genetic and geographic proximity; analysis of correspondences relates localities and genetic systems in an eigenvectorial space; and geographic mapping displays the principal components of gene frequencies in the real space. In 1,364 adults in 26 residential units, seven presumably neutral isoenzyme systems were typed; ACP1 ESD, GLO I, GPT, PGD, PGM1 and PGP. It was found that average kinship for these neutral systems is correlated with geographic distance in this small area, but not as strongly as kinship for beta-thalassemia. A north-south gradient was observed for ESD. Analysi...
The restriction fragment length polymorphism (RFLP) haplotypes of cystic fibrosis (CF) alleles va... more The restriction fragment length polymorphism (RFLP) haplotypes of cystic fibrosis (CF) alleles vary between populations. To determine the distribution of two RFLPs (XV-2C and KM-19) that are tightly linked to the CF locus, we analyzed a white sample from five different states of Brazil. The haplotypes of 314 CF- and 237 non-CF-bearing chromosomes were uniformly distributed over the five states. The XV-2C allele and haplotype frequencies and the degree of linkage disequilibrium were determined. These were similar to values previously reported in southern European countries but different from results reported for northern and central Europe and North America. In contrast, although KM-19 allele frequencies differed between Brazilian states and European and North American countries, these frequencies were similar to values reported in black Americans. A significant proportion of Brazilian CF-bearing chromosomes had less common haplotypes, suggesting a heterogeneous distribution of CF gene mutations among Brazilians. Further studies are needed to identify the mutations affecting the Brazilian CF patients with various haplotypes.
Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 1996
Some data suggest that cesarean section reduces mother-to-child HIV-1 transmission. To assess the... more Some data suggest that cesarean section reduces mother-to-child HIV-1 transmission. To assess the influence of mode of delivery and other maternal and infant factors on the rate of transmission, we analyzed the data of 1,624 children prospectively followed from birth. Of these, at the last visit 1,033 were &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; 18 months of age or would have been had they not died of HIV-related illness. Among the 975 first singleton children, 180 [18.5%; 95% confidence limits (CL), 16.1-20.9] acquired infection, as did 8 of 56 (14.3%; 95% CL, 5.1-23.5) second-born children. Multivariate stepwise analysis showed that vaginal delivery and development of symptoms in the mother were significantly and independently associated with a higher transmission rate (vaginal delivery; odds ratio, 1.69; 95% CL, 1.14-2.5; symptoms: odds ratio, 1.61; 95% CL, 1.12-2.3). In contrast, a history of maternal drug use, birth weight, breast-feeding (only 37 infants were breast-fed), and child&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s sex did not have a significant impact on viral transmission. The percentage of infected children was highest (30.7%) among very premature infants (&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; or = 32 weeks of gestation); this significant trend subsequently decreased to 11.9% at the week 42 (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001), suggesting a parallel reduction in peripartum transmission. The reduced rate of infection observed in infants born by cesarean section underlines the urgent need for randomized controlled trials to evaluate the protective role of surgical delivery in preventing perinatal HIV-1 transmission.
Tissue Antigens, 1993
association of HSP70-1 promoter region alleles and their inclusion in extended HLA haplotypes.
Tissue Antigens, 1995
Trino Vercellese, a village of Piedmont (Italy), was selected with the aim at reconstructing the ... more Trino Vercellese, a village of Piedmont (Italy), was selected with the aim at reconstructing the genetic history of a putative Celtic sample known to be settled in Italy with the name of Rigornagus since pre-roman times. The HLA-A, Cw, B, DR and DQ antigens of 101 unrelated individuals have been typed. The antigens characterizing this sample for their higher frequency are shown to be A3,
Aim. Our aim is to define the trends in the hazard of overall relapse and pattern of the first re... more Aim. Our aim is to define the trends in the hazard of overall relapse and pattern of the first recurrence as functions of time elapsed from diagnosis and to identify the parameters with independent predictive value on the relapse risk in stage I-II melanoma patients according to the revised AJCC classification. Methods. A total of 3 174 stage I-II melanoma patients diagnosed and prospectively followed-up since 1975 have been reviewed as to disease course over time. Results. A significant increase in the annual hazard of relapse was found for each time interval from stage IA to IIB/IIC in the first follow-up decade. The incidence of late metastases was significantly higher in patients with thicker melanoma than in those with thinner melanoma. Distant relapses showed a low (<1.5%), but constant annual incidence as first site of recurrence. The lower limb location showed a <1% interval incidence of visceral metastases as first site of relapse, irrespectively of the AJCC stage, compared to 4.7% for the other body sites. Multivariate analysis of DFS showed that Breslow thickness, presence of ulceration and a primary location to foot carry an independent unfavourable prognostic significance. Conclusion. The AJCC stage is associated with the incidence and time course behaviour of the first relapse, the primary location mainly to the pattern of the first relapse.
Journal of Hepatology, 1999
Background/Aim: Hepatic arteriovenous shunting in the metastatic liver reduces the advantages of ... more Background/Aim: Hepatic arteriovenous shunting in the metastatic liver reduces the advantages of intraarterial infusion of chemotherapeutic agents because of the passage of drugs into the systemic circulation. The aim of this study was to quantitatively assess spontaneous functional hepatic arteriovenous shunting in patients with liver metastases and to determine its implication in the increase in systemic toxic effects of intra-arterial infusion chemotherapy with floxuridine. Methods: Twenty-five patients who underwent implantation of arterial ports for regional chemotherapy of liver metastases were studied. Functional hepatic arterio-venous shunting was evaluated through the bioavailability of intra-arterially administered D-sorbitol, a safe, natural compound whose kinetic features make its hepatic clearance flow dependent. In addition, D-sorbitol hepatic clearance (a parameter reflecting functional liver blood flow) and common liver function tests were evaluated for each studied patient. Patients were then grouped with respect to the percentage of medically-assessed liver occupation by metastases and with respect to systemic toxicity of the chemotherapeutic treatment. Both univariate and multivariate analyses by Student's t-test and stepwise logistic regression, respectively, were performed in both groups for each of the evaluated parameters S EVERAL STUDIES have been performed to date to assess the relative contribution of portal and arterial blood supply to the perfusion of hepatic metastases,
Journal of Endocrinological Investigation, 2010
The objective of this study is to determine the optimal conditions for human semen incubation tre... more The objective of this study is to determine the optimal conditions for human semen incubation treated with exogenous platelet activating factor (ePAF) for intra-uterine insemination (IUI). This prospective study was carried out on 32 infertile men and each semen sample was processed with the ISolate Sperm Separation Medium, washed with sperm washing medium (SWM) and resuspended either in SWM alone (control samples), or with ePAF 0.1, 0.5, and 1.0 μM. Each concentration was subsequently incubated and evaluated at 5, 15, 30, and 60 min. The motility parameters were evaluated by the computer-aided sperm analysis (C.A.S.A.) system. Curvilinear velocity, straight line velocity, average path velocity, rapid and progressive motility significantly increased compared to control samples at an ePAF concentration of 0.1 μM (with at least 15 min of incubation). The best results were obtained with ePAF concentrations of: 0.1 μM (60 min of incubation) and 0.5 μM (30-60 min of incubation). In conclusion, results are enhanced when ePAF is added to standard semen preparation for IUI. An ePAF concentration of 0.1 μM, with an incubation time of 15 min, can be used for semen samples with normal motility. Whilst, for semen samples with poor motility, the ePAF concentration is best increased to 0.5 μM and/or the incubation time prolonged to 60 min.
Journal of Clinical Oncology, 2007
... Pietro Quaglino, Simona Osella-Abate, Paola Savoia, Maria Grazia Bernengo. Department of Biom... more ... Pietro Quaglino, Simona Osella-Abate, Paola Savoia, Maria Grazia Bernengo. Department of Biomedical Sciences and Human Oncology, Section of ... 1. Scoggins CR, Ross MI, Reitgen DS, et al: Prospective multi-institutional study of reverse transcriptase polymerase chain ...
International Journal of Immunogenetics, 1992
SUMMARYHLA‐DQ genes and gluten diet are the main factors involved in the pathogenesis of Dermatit... more SUMMARYHLA‐DQ genes and gluten diet are the main factors involved in the pathogenesis of Dermatitis Herpetiformis (DH), as well as Coeliac Disease (CD). However other genetic factors are probably relevant, since about 10% of the patients with DH and CD lack the DQA1*0501/B1*0201 heterodimer while the majority of individuals presenting this genotype and also being exposed to gluten diets did not suffer from these diseases. To evaluate the role of other genes, 36 Northern Italian children with DH were analysed for DNA polymorphisms at HLA‐DP and immunoglobulin (Ig) heavy chain loci. DPA1*0201 and DPB1*1301 frequencies were higher in patients than in controls (Pc= 0.0357 and Pc= 0.0273). With respect to immunoglobulin heavy chain restriction fragment length polymorphisms (RFLP), the 4.6kb Sad RFLP at the switch α2 gene was more frequent in patients (0.13) than in controls (0.019; Pc= 0.036). Moreover, rare alleles or duplications in the switch regions occurred more frequently in the pa...
Infection, 1991
Summary In order to establish a correlation with disease progression we prospectively evaluated t... more Summary In order to establish a correlation with disease progression we prospectively evaluated ten clinical and immunologic parameters in 102 consecutive HIV-positive subjects. The eight immunologic variables were:in vitro spontaneous interferon release by peripheral blood monocytic cells, alpha- and gamma-interferon production induced by Newcastle Disease Virus and PHA, Multitest Mérieux score, PHA- and CON-A-induced lymphocyte transformation, absolute number of CD4+
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Papers by Nazario Cappello