Papers by Nathaly Mendoza
SummaryThe difficulty of retrieving high-resolution,in vivoevidence of the proliferative- and mig... more SummaryThe difficulty of retrieving high-resolution,in vivoevidence of the proliferative- and migratory processes occurring in neural germinal zones has limited our understanding of neurodevelopmental mechanisms. Here, we employed a connectomic approach using a high-resolution, serial-sectioning scanning electron microscopy volume to investigate the laminar cytoarchitecture of the transient external granular layer (EGL) of the developing cerebellum, where granule cells coordinate a series of mitotic and migratory events. By integrating image segmentation, 3D reconstruction, and deep learning approaches, we discovered and characterized anatomically complex intercellular connections bridging pairs of cerebellar granule cells throughout the EGL. Connected cells were either mitotic, migratory, or transitioning between these two cell stages, displaying a chronological continuum of proliferative and migratory events never previously observedin vivoat this resolution. This unprecedented ul...
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Papers by Nathaly Mendoza