Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in tra... more Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in transthoracic fine needle biopsy (TFNB) aspirates collected from peripheral tumours of the lung in predicting the malignant aetiology of lung infiltrations. Methods: 100 patients with a peripheral infiltration of the lung underwent TFNB of the focal lesion. The aspirates were subjected to standard cytological evaluation. Telomerase activity in the specimens was determined with the PCR-ELISA PLUS method. The sensitivity, specificity, accuracy and predictive value of TFNB were calculated for cytological examination of aspirates alone and cytological examination with additional telomerase activity assessment. Results: Lung cancer was newly diagnosed in 84 subjects and benign peripheral lesions were found in 16. During the first TFNB, lung cancer was identified in 56 cases of cancer (66.7%) while increased telomerase activity was found in 61 cancer aspirates (72.6%). No subject with a benign infiltration had a false positive result from cytological examination, but in one case (6.25%) increased telomerase activity was observed. The diagnostic sensitivity, accuracy and negative predictive value of the combination of cytological examination and telomerase activity assay in TFNB specimens were significantly higher than for cytological examination alone (89.3% vs 66.7%, p = 0.0004; 90% vs 72%, p = 0.001; 62.5% vs 36.4%, p = 0.039), but a combination of the two examinations was associated with a lower specificity of TFNB (96.9% vs 100%, p = 0.002). Conclusion: Detection of telomerase activity in aspirates taken during TFNB of a peripheral lung infiltration should be considered as an indication of the risk of malignancy in cases with false negative cytological results.
i Alergologii, kierownik: prof. dr hab. med. T. Płusa 2 Zakład Radiologii kierownik: dr hab. med.... more i Alergologii, kierownik: prof. dr hab. med. T. Płusa 2 Zakład Radiologii kierownik: dr hab. med. P. Twarkowski z Mazowieckiego Centrum Chorób Płuc i Gruźlicy, Otwock. 3 Pracownia Patomorfologii: kierownik dr med. N.H. Qandil, 4 Oddział Torakochirurgii: kierownik dr med. J. Krusiewicz Summary: Authors present a case of EH as an accidental finding in 40 year old woman appeared as the presented small, solid nodules in both lungs on a routine chest radiograph and HRCT scans. There were no signs in clinical examination, no abnormalities were presented in the spirometric and respiratory tests, neither in the endoscopic digestive tract examinations nor in CT scans of abdomen. The histological examination of open lung biopsy revealed the morphology of epithelioid haemangioendothelioma. During one year follow up no clinical nor radiological progressions have been observed.
Introduction: In many cases typing of advanced lung non-small cell carcinoma (NSCLC), especially ... more Introduction: In many cases typing of advanced lung non-small cell carcinoma (NSCLC), especially differentiation between adenocarcinoma (ADC) and squamous cell carcinoma (SCC), requires special stains, mainly immunohistochemistry (IHC). Methods: 53 cases of NSCLC diagnosed on small biopsies and cell block samples were evaluated. The panel of IHC antibodies comprised markers characteristic for ADC (TTF-1, napsin A, CK7) and for SCC (p63, CK5/6, CK34ßE12) in order to determine their value in diagnosis of NSCLC. Besides IHC, mucicarmine stain was tested. Results were correlated with the final diagnosis obtained from surgical specimens. Results: In 57% of cases the subtype of NSCLC was established only by H+E stain. Using mucin stain and immunohistochemistry the accuracy of diagnosis increased by 22% to reach 79%. P63 turned out to be the most specific antibody, whose expression was seen in the vast majority of SQCC and in a squamous component of other types of NSCLC. CK5/6 exhibited si...
The primary tracheo-bronchial amyloidosis is a rare entity with long lasting and progressive cour... more The primary tracheo-bronchial amyloidosis is a rare entity with long lasting and progressive course. Precise diagnosis can be established on the basis of pathological features seen in samples derived from the airways, obtained during fibreoptic bronchoscopy or during lung biopsy sometimes. Describing the case of an insidious course of primary tracheobronchial AL type amyloidoisis, that was initially recognised and treated as a chronic obstructive pulmonary disease. The authors represent its clinical course and the diagnostic difficulties. The changes in the respiratory tract, both radiological and endoscopic suggested a tuberculous or proliferative process. They were responsible for a severe increasing dyspnoea, due to bronchial obstruction, with muco-haemoptic expectoration. A forceps resection of the endobronchial lesions enabled to established the right diagnosis and further treatment limited to local procedures.
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2003
The affinity of various malignant neoplasms including small cell and non-small cell lung cancer f... more The affinity of various malignant neoplasms including small cell and non-small cell lung cancer for peptide analogue of somatostatin has been documented. Depreotide is such an analogue and can be combined with technetium--99m (99mTc depreotide) for optical imaging properties. Using this radiopharmaceutical product, solitary pulmonary nodules (SPN) were previously successful evaluated. In this article the preliminary study of the depreotide (NeoSpect--Amersham, Wielka Brytania) in clinical practice has been shown.
Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in tra... more Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in transthoracic fine needle biopsy (TFNB) aspirates collected from peripheral tumours of the lung in predicting the malignant aetiology of lung infiltrations. Methods: 100 patients with a peripheral infiltration of the lung underwent TFNB of the focal lesion. The aspirates were subjected to standard cytological evaluation. Telomerase activity in the specimens was determined with the PCR-ELISA PLUS method. The sensitivity, specificity, accuracy and predictive value of TFNB were calculated for cytological examination of aspirates alone and cytological examination with additional telomerase activity assessment. Results: Lung cancer was newly diagnosed in 84 subjects and benign peripheral lesions were found in 16. During the first TFNB, lung cancer was identified in 56 cases of cancer (66.7%) while increased telomerase activity was found in 61 cancer aspirates (72.6%). No subject with a benign infiltration had a false positive result from cytological examination, but in one case (6.25%) increased telomerase activity was observed. The diagnostic sensitivity, accuracy and negative predictive value of the combination of cytological examination and telomerase activity assay in TFNB specimens were significantly higher than for cytological examination alone (89.3% vs 66.7%, p = 0.0004; 90% vs 72%, p = 0.001; 62.5% vs 36.4%, p = 0.039), but a combination of the two examinations was associated with a lower specificity of TFNB (96.9% vs 100%, p = 0.002). Conclusion: Detection of telomerase activity in aspirates taken during TFNB of a peripheral lung infiltration should be considered as an indication of the risk of malignancy in cases with false negative cytological results.
Authors present a case of EH as an accidental finding in 40 year old woman appeared as the presen... more Authors present a case of EH as an accidental finding in 40 year old woman appeared as the presented small, solid nodules in both lungs on a routine chest radiograph and HRCT scans. There were no signs in clinical examination, no abnormalities were presented in the spirometric and respiratory tests, neither in the endoscopic digestive tract examinations nor in CT scans of abdomen. The histological examination of open lung biopsy revealed the morphology of epithelioid haemangioendothelioma. During one year follow up no clinical nor radiological progressions have been observed.
Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in tra... more Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in transthoracic fine needle biopsy (TFNB) aspirates collected from peripheral tumours of the lung in predicting the malignant aetiology of lung infiltrations. Methods: 100 patients with a peripheral infiltration of the lung underwent TFNB of the focal lesion. The aspirates were subjected to standard cytological evaluation. Telomerase activity in the specimens was determined with the PCR-ELISA PLUS method. The sensitivity, specificity, accuracy and predictive value of TFNB were calculated for cytological examination of aspirates alone and cytological examination with additional telomerase activity assessment. Results: Lung cancer was newly diagnosed in 84 subjects and benign peripheral lesions were found in 16. During the first TFNB, lung cancer was identified in 56 cases of cancer (66.7%) while increased telomerase activity was found in 61 cancer aspirates (72.6%). No subject with a benign infiltration had a false positive result from cytological examination, but in one case (6.25%) increased telomerase activity was observed. The diagnostic sensitivity, accuracy and negative predictive value of the combination of cytological examination and telomerase activity assay in TFNB specimens were significantly higher than for cytological examination alone (89.3% vs 66.7%, p = 0.0004; 90% vs 72%, p = 0.001; 62.5% vs 36.4%, p = 0.039), but a combination of the two examinations was associated with a lower specificity of TFNB (96.9% vs 100%, p = 0.002). Conclusion: Detection of telomerase activity in aspirates taken during TFNB of a peripheral lung infiltration should be considered as an indication of the risk of malignancy in cases with false negative cytological results.
i Alergologii, kierownik: prof. dr hab. med. T. Płusa 2 Zakład Radiologii kierownik: dr hab. med.... more i Alergologii, kierownik: prof. dr hab. med. T. Płusa 2 Zakład Radiologii kierownik: dr hab. med. P. Twarkowski z Mazowieckiego Centrum Chorób Płuc i Gruźlicy, Otwock. 3 Pracownia Patomorfologii: kierownik dr med. N.H. Qandil, 4 Oddział Torakochirurgii: kierownik dr med. J. Krusiewicz Summary: Authors present a case of EH as an accidental finding in 40 year old woman appeared as the presented small, solid nodules in both lungs on a routine chest radiograph and HRCT scans. There were no signs in clinical examination, no abnormalities were presented in the spirometric and respiratory tests, neither in the endoscopic digestive tract examinations nor in CT scans of abdomen. The histological examination of open lung biopsy revealed the morphology of epithelioid haemangioendothelioma. During one year follow up no clinical nor radiological progressions have been observed.
Introduction: In many cases typing of advanced lung non-small cell carcinoma (NSCLC), especially ... more Introduction: In many cases typing of advanced lung non-small cell carcinoma (NSCLC), especially differentiation between adenocarcinoma (ADC) and squamous cell carcinoma (SCC), requires special stains, mainly immunohistochemistry (IHC). Methods: 53 cases of NSCLC diagnosed on small biopsies and cell block samples were evaluated. The panel of IHC antibodies comprised markers characteristic for ADC (TTF-1, napsin A, CK7) and for SCC (p63, CK5/6, CK34ßE12) in order to determine their value in diagnosis of NSCLC. Besides IHC, mucicarmine stain was tested. Results were correlated with the final diagnosis obtained from surgical specimens. Results: In 57% of cases the subtype of NSCLC was established only by H+E stain. Using mucin stain and immunohistochemistry the accuracy of diagnosis increased by 22% to reach 79%. P63 turned out to be the most specific antibody, whose expression was seen in the vast majority of SQCC and in a squamous component of other types of NSCLC. CK5/6 exhibited si...
The primary tracheo-bronchial amyloidosis is a rare entity with long lasting and progressive cour... more The primary tracheo-bronchial amyloidosis is a rare entity with long lasting and progressive course. Precise diagnosis can be established on the basis of pathological features seen in samples derived from the airways, obtained during fibreoptic bronchoscopy or during lung biopsy sometimes. Describing the case of an insidious course of primary tracheobronchial AL type amyloidoisis, that was initially recognised and treated as a chronic obstructive pulmonary disease. The authors represent its clinical course and the diagnostic difficulties. The changes in the respiratory tract, both radiological and endoscopic suggested a tuberculous or proliferative process. They were responsible for a severe increasing dyspnoea, due to bronchial obstruction, with muco-haemoptic expectoration. A forceps resection of the endobronchial lesions enabled to established the right diagnosis and further treatment limited to local procedures.
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2003
The affinity of various malignant neoplasms including small cell and non-small cell lung cancer f... more The affinity of various malignant neoplasms including small cell and non-small cell lung cancer for peptide analogue of somatostatin has been documented. Depreotide is such an analogue and can be combined with technetium--99m (99mTc depreotide) for optical imaging properties. Using this radiopharmaceutical product, solitary pulmonary nodules (SPN) were previously successful evaluated. In this article the preliminary study of the depreotide (NeoSpect--Amersham, Wielka Brytania) in clinical practice has been shown.
Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in tra... more Background: A study was undertaken to evaluate the usefulness of telomerase activity assay in transthoracic fine needle biopsy (TFNB) aspirates collected from peripheral tumours of the lung in predicting the malignant aetiology of lung infiltrations. Methods: 100 patients with a peripheral infiltration of the lung underwent TFNB of the focal lesion. The aspirates were subjected to standard cytological evaluation. Telomerase activity in the specimens was determined with the PCR-ELISA PLUS method. The sensitivity, specificity, accuracy and predictive value of TFNB were calculated for cytological examination of aspirates alone and cytological examination with additional telomerase activity assessment. Results: Lung cancer was newly diagnosed in 84 subjects and benign peripheral lesions were found in 16. During the first TFNB, lung cancer was identified in 56 cases of cancer (66.7%) while increased telomerase activity was found in 61 cancer aspirates (72.6%). No subject with a benign infiltration had a false positive result from cytological examination, but in one case (6.25%) increased telomerase activity was observed. The diagnostic sensitivity, accuracy and negative predictive value of the combination of cytological examination and telomerase activity assay in TFNB specimens were significantly higher than for cytological examination alone (89.3% vs 66.7%, p = 0.0004; 90% vs 72%, p = 0.001; 62.5% vs 36.4%, p = 0.039), but a combination of the two examinations was associated with a lower specificity of TFNB (96.9% vs 100%, p = 0.002). Conclusion: Detection of telomerase activity in aspirates taken during TFNB of a peripheral lung infiltration should be considered as an indication of the risk of malignancy in cases with false negative cytological results.
Authors present a case of EH as an accidental finding in 40 year old woman appeared as the presen... more Authors present a case of EH as an accidental finding in 40 year old woman appeared as the presented small, solid nodules in both lungs on a routine chest radiograph and HRCT scans. There were no signs in clinical examination, no abnormalities were presented in the spirometric and respiratory tests, neither in the endoscopic digestive tract examinations nor in CT scans of abdomen. The histological examination of open lung biopsy revealed the morphology of epithelioid haemangioendothelioma. During one year follow up no clinical nor radiological progressions have been observed.
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