Papers by Nahid Einollahi
PubMed, Feb 3, 2011
Leptin, a circulating 16-kd polypeptide consisting of 167 amino acids, appears to be involved in ... more Leptin, a circulating 16-kd polypeptide consisting of 167 amino acids, appears to be involved in the body weight homeostasis. Moreover leptin plays an important role for the reproductive system, early embryogenesis, and fat metabolism during pregnancy and puberty. Significant correlations have been found between leptin and sexual hormones, which is a cytokine and has hormonal properties. The aim of this study was to determine serum leptin levels during the menstrual cycle, and the association between serum leptin and reproductive hormones in young, healthy Iranian women. 42 healthy women volunteered for the study. They all had regular menstrual cycles, with cycle length varying between 26 and 32 days. None of them used oral contraceptives. All were of normal weight, with body mass index ( BMI) < 25 Kg/m2. Fasting blood samples were collected during the follicular phase, mid cycle and luteal phase of the menstrual cycle. FSH and LH were measured with coated tube immunoradiometric assay. Estrogen and progesterone were measured using antibody -coated tubes. Serum Leptin concentration were measured by Leptin (sandwich) ELISA. In menstruating women, serum leptin increased from 13.15+/-1.60 ng/ml in the early follicular phase to 16.57+/-1.68 ng/ml (P<0.01) at the luteal phase. Serum leptin concentration negatively correlated with LH and progesterone (P<0.05). Mean serum leptin levels correlated with body mass index (BMI) (r =0.78, P<0.001).
Journal of Payavard Salamat, Jan 10, 2017
African Journal of Biotechnology, Apr 3, 2012
To investigate the effect of exogenous nitric oxide donor (PAPA NONOate) a drug which spontaneous... more To investigate the effect of exogenous nitric oxide donor (PAPA NONOate) a drug which spontaneously release nitric oxide on the rate of wound healing and collagen synthesis on impaired wound healing in experimental diabetes. 12 male Sprague-Dawley rats were transferred to separate metabolic cages. Nine days before wounding, the rats were injected intraperitoneally (IP) with streptozotocin (STZ) (55 mg/kg body weight in citrate buffer 0.1 mol/L, pH 4.5) to induce diabetes. The dorsal surface of each rat was properly shaved and given full thickness dermal wound. The test group (n = 6) was treated with 100 µmole PAPA NONOate in phosphate buffer while control wounds (n = 6) received sterile phosphate buffer on the same day and every three days. Daily urine samples were collected at every 24 h intervals. To inhibit bacterial growth, 5 ml of 3 M HCl was added to each urine collection (pH = 1) and urine samples were kept frozen until analyzed (-70°C). Urinary nitrate (NO-3) was quantitated daily prior to wounding, and during wound healing (21 days) following external wound closure. The rate of wound healing was determined by video image analysis. PAPA NONOate treatment increased the rate wound healing in test group as compared to the control group. The nitric oxide donor PAPA NONOate may represent a potential treatment for impaired wound healing in diabetes by increasing the rate of collagen synthesis at the wound site.
مجله دانشکده دندانپزشکی اصفهان, Mar 2, 2015
Laboratory & Diagnosis, Sep 10, 2016
Laboratory & Diagnosis, Mar 10, 2019
Diagnostics, Apr 3, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
DOAJ (DOAJ: Directory of Open Access Journals), Oct 1, 2021
Payavard Salamat, Jan 10, 2021
KAUMS Journal, 2013
Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell i... more Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell is about 0.1 g per ton. Several studies have proven silver nitrate can produce ROS (oxygen reactive species) and harm cells through a variety of mechanisms including interactions with enzymes and proteins sulphydryl groups. Major sources of Silver in surface waters is natural leaching, human activities, such as mining, manufacture of silverware and jewellery, photografic manufacturing and processing which can elevate silver levels in aquatic environments. Materials and Methods: In This study horseradish peroxidase (HRP) activity was determinded under steady-state kinetic conditions. Incubation of the enzyme with 1-100mM silver nitrate for 1-60 min in room temperature resulted in dual effects. Results: Progressive inhibition of the enzymatic activity was observed after 30-60 min enzyme incubation with 0.05-100 mM of Ag+. After 30 and 60 min incubation time, enzyme inhibition was (35-60%) a...
DOAJ (DOAJ: Directory of Open Access Journals), Sep 1, 2009
DOAJ (DOAJ: Directory of Open Access Journals), Apr 1, 2010
DOAJ (DOAJ: Directory of Open Access Journals), Oct 1, 2009
Tehran University …, 2010
Background: JAK2 is a nonreceptor tyrosine kinase that plays a major role in myeloid disorders. T... more Background: JAK2 is a nonreceptor tyrosine kinase that plays a major role in myeloid disorders. This mutation is characterized by a G to T transverse at nucleotide 1849 in exon 12 of the JAK2 gene, located on the chromosome 9p, leading to a substitution of valine to phenylalanine at amino acid position 617 in the JAK2 protein. In this study we compared the amplification refractory mutation (ARMS) assay and allele-specific (AS-PCR) to evaluate JAK2V617F mutation patients with non-CML myeloproliferative neoplasms (MPNS). Methods: In this experimental study we evaluated JAK2 mutation in 58 patients with a known or suspected diagnosis of a myeloproliferative neoplasm by simple randomized sampling. The mutation was detected by ARMS-PCR and AS-PCR in patients. In order to verify the methods, amplified products from some patients were sequenced. Results: The JAK2 V617F mutation was detected in 86.6%(26/30) of patients with polycythemia vera and 61.5%(8/13) of patients with idiopathic myelofibrosis by ARMS-PCR and AS-PCR. 46.6%(7.15) of essential thrombocythemia patients were positive using ARMS-PCR method while 53%(8.15) of then were positive when AS-PCR were used. The mutation was confirmed by sequencing. Conclusions: The incidence of JAK2 mutation using above PCR methods is similar to previous studies. The different results may depend on the molecular technique used.
Mercury is one of the three major environmental metal poisons, and mercuric chloride is a highly ... more Mercury is one of the three major environmental metal poisons, and mercuric chloride is a highly reactive compound which can harm cells by a variety of mechanisms including direct interaction with sulphydryl groups of proteins and enzymes, therefore affecting the enzymatic activity. This study focused on the effect of Hg ++ on horseradish peroxidase (donor: hydrogen peroxide oxidoreductase, EC 1.11.1.7) (HRP) (Isoenzyme C) activity. In the presence of 88 mM hydrogen peroxide K m for o-dianisidine oxidation was 0.05 millimolar and V max was 8.5 µM.s-1. Incubation of the enzyme with 1 to 100 millimolar mercuric chloride for 5-20-and 60 min resulted in progressive inhibition of the enzymatic activity. At low Hg ++ concentrations the inhibition was reversible by excess substrate, while at high Hg ++ concentration the inhibition was not reversible. Results also indicated that the type of inhibition depended on the duration of incubation of the enzyme with metal ion and on the Hg ++ concentration. So we could conclude that the type of inhibition changed from noncompetitive to mix with increased incubation time and increased metal concentration.
The Medical Journal of The Islamic Republic of Iran, 1991
Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient me... more Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A2601A2so=2). In addition we have applied the same technique for the first time to purify the mitochondrial DNA of platelets.
Disease and Diagnosis
Background: The polymorphism associated with liver fat content, which is well-known as PNPLA3 rs7... more Background: The polymorphism associated with liver fat content, which is well-known as PNPLA3 rs738409 (Patatin-like phospholipase domain-containing protein 3), is one of the critical subjects widely investigated in the literature regarding the prevalence of non-alcoholic fatty liver disease (NAFLD) worldwide. The present research aimed to study the bioinformatics investigations of this polymorphism together with the in vitro analyses among patients with NAFLD. Materials and Methods: In this case-control study, after performing bioinformatics analysis, the laboratory examination was performed in several steps. Genomic DNA was extracted from the blood of 53 NAFLD patients and 107 subjects with normal liver ultrasounds. PNPLA3 rs738409 was genotyped by the polymerase chain reaction-restriction fragment length polymorphism method. The laboratory test results, including fasting blood sugar, triglyceride, cholesterol, high-density lipoprotein, low-density lipoprotein, alanine aminotransf...
مجله دانشکده پزشکی اصفهان, 2010
مقدمه: در سال 2005، چندین گروه شیوع بالایی از جهش V617F (G→T) را در ژن تیروزین کیناز JAK2 در نئوپ... more مقدمه: در سال 2005، چندین گروه شیوع بالایی از جهش V617F (G→T) را در ژن تیروزین کیناز JAK2 در نئوپلاسمهای میلوپرولیفراتیو مزمن (MPNs) شناسایی کردند. در مطالعهی حاضر میزان شیوع این جهش و ارتباط آن با یافتههای آزمایشگاهی و بالینی در بیماران مبتلا به این نئوپلاسمها مورد ارزیابی قرار گرفت. روشها: جهش JAK2 V617F با روش سیستم تکثیر متزلزل جهشها (ARMS-PCR) در 92 بیمار با تشخیص جدید یا در حال درمان مبتلا به نئوپلاسمهای میلوپرولیفراتیو مزمن (MPNs) از طریق نمونه گیری تصادفی ساده مورد بررسی قرار گرفت. یافتهها: جهش در 6/86 درصد (30/26) بیماران پلیسیتمی ورا، 6/46 درصد (15/7) بیماران ترومبوسیتمی اولیه، 5/61 درصد (13/8) بیماران میلوفیبروز اولیه و 14 درصد (34/4) بیماران لوسمی میلوئید مزمن شناسایی شد. بیماران جهش مثبت پلیسیتمی ورا، میزان گلبولهای سفید بالاتری داشتند (03/0 = P). به علاوه، از 26 بیمار پلیسیتمی ورا JAK2 مثبت، 16 بیمار زن بودند و 17 بیمار اسپلنومگالی داشتند. یک بیمار پلیسیتمی ورا به طور همزمان دارای جهش JAK2 و ناهنجاری کروموزومی فیلادلفیا بود. در سایر گروهها تفاوت قابل توج...
Color-blindness is the inability to perceive differences between some color that other people can... more Color-blindness is the inability to perceive differences between some color that other people can distinguish. Using a literature search , the results indicate the prevalence of color vision deficiency in the medical profession and its on medical skills. Medical laboratory technicians and technologists employees should also screen for color blindness. This research aimed to study color blindness prevalence among Hospitals' Clinical Laboratories' Employees and Students in Tehran University of Medical Sciences (TUMS). A cross-sectional descriptive and analytical study was conducted among 633 TUMS Clinical Laboratory Sciences' Students and Hospitals' Clinical Laboratories' Employees to detect color-blindness problems by Ishihara Test. The tests were first screened with certain pictures, then compared to the Ishihara criteria to be possible color defective were tested further with other plates to determine color-blindness defects. The data was saved using with SPSS s...
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Papers by Nahid Einollahi