Papers by Mustafa Kemal sarıbay
Polish Journal of Veterinary Sciences, Jul 26, 2023
This study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide ad... more This study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), activities of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase (GPx) in subclinical mastitic cows. Milk from each udder was collected and grouped by the California Mastitis Test. Then, a somatic cell count (SCC) was performed, and the groups were re-scored as control (5-87 × 10 3 cells), 1 st group (154-381 × 10 3 cells), 2 nd group (418-851 × 10 3 cells), 3 rd group (914-1958 × 10 3 cells), and 4 th group (2275-8528 × 10 3 cells). Milk cell TP, NADPH, tGSH levels, G6PD, and GPx activities were assessed. Microbiological diagnosis and aerobic mesophyle general organism (AMG, cfu/g) were also conducted. In mastitic milk, TP, NADPH, and tGSH levels, and G6PD and GPx activities were significantly reduced per cell (in samples of 10 6 cells). In addition, milk SCC was positively correlated with AMG (r=0.561, p<0.001), NADPH (r=0.380, p<0.01), TP (r=0.347, p<0.01) and G6PD (r=0.540, p<0.001). There was also positive correlation between NADPH (r=0.428, p<0.01), TP (r=0.638, p<0.001) and AMG. NADPH was positively correlated with TP (r=0.239, p<0.05), GPx (r=0.265, p<0.05) and G6PD (r=0.248, p=0.056). Total protein was positively correlated with tGSH (r=0.354, p<0.01) and G6PD (r=0.643, p<0.001). There was a negative correlation between tGSH and GPx activity (r=-0.306, p<0.05). The microbiological analysis showed the following ratio of pathogens: Coagulase-Negative Staphylococci 66.6%, Streptococcus spp 9.5%, Bacillus spp 9.5%, yeast 4.8%, and mixed infections 9.5%. As a conclusion, when evaluating the enzyme and oxidative stress parameters in milk, it is more suitable to assign values based on cell count rather than ml of milk. The linear correlation between the SCC and AMG, milk cell NADPH, TP and G6PD suggests that these parameters could be used as markers of mastitis.
Fırat Üniversitesi Sağlık Bilimleri Dergisi, 2007
Atatürk üniversitesi veteriner bilimleri dergisi, Oct 30, 2016
This study was performed to determine the prevalance, causative microorganisms and antimicrobial ... more This study was performed to determine the prevalance, causative microorganisms and antimicrobial susceptibility of microorganisms in goat subclinical mastitis around Hatay. A total of 1010 mammary halves of 505 goats were examined by California Mastitis Test (CMT). The somatic cell counts (SCC) were determined by microscopic method. Isolation and identification of microorganisms were carried out by conventional microbiologic methods. Staphylococci were further differentiated by API-STAPH system. Antimicrobial susceptibility was determined by disc diffusion method. The prevalance of the subclinical mastitis was found 8.71 %. The most prevalent microorganism was staphylococci (71.5%). Microorganisms except Staphylococci were Streptococci (8%), Bacillus spp. (5.7%), Escherischia coli (4.5%), Corynebacterium spp. (3.4%), Pseudomonas spp. (2.3%) and Acinetobacteri spp. (2.3%). In addition, mix infection was defined in 2.3% of samples. Highly resistance was found against penicilin, erythromycin, oxytetracycline, gentamicin, amoxicillin. Slightly resistance was found against enrofloxacin, amoxicillin-clavulonic acid, kanamycin plus cephalexin and there was no resistance against cefalotin. It was concluded that, prevalance of subclinical mastitis should be cared; also staphylococci especially Coagulase negative staphylococci are the most commonly isolated bacteria in subclinical mastitis around Hatay. In goat mastitis diagnosis strong positive CMT results should be cared. CMT and SCC results should be supported with microbiologic tests.
İz mineraller canlının yaşam fonksiyonlarının yerine getirilmesi ve fizyolojik işleyişin süreklil... more İz mineraller canlının yaşam fonksiyonlarının yerine getirilmesi ve fizyolojik işleyişin sürekliliğinde son derece önemlidirler. İz mineraller, hücrelerin, hormonların ve vücut enzimlerinin esansiyel yapı taşlarıdır. İz minerallerin eksiklikleri, süt inekçiliğinde karlılık için hayati önemi olan gebeliğin oluşması, devam ettirilmesi ve sağlıklı bir şekilde sonlanması ile doğum sonrası en kısa sürede hayvanın yeniden gebe kalmasında aksaklıkların ortaya çıkmasına neden olabilmektedir. Bu derlemede, sütçü ineklerde fertilitenin optimum seviyede tutulması için gerekli olan ve dışarıdan alınması zorunlu en önemli iz minerallerden, bakır (Cu), çinko (Zn) ve selenyum (Se) incelenmiştir ve fertilitedeki önemi ortaya konulmuştur
Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, Jun 12, 2018
Repeat breeder sendromu (RB) sütçü ineklerde doğum-yeniden gebe kalma aralığını olumsuz yönde etk... more Repeat breeder sendromu (RB) sütçü ineklerde doğum-yeniden gebe kalma aralığını olumsuz yönde etkilediği için önemli bir sorundur. Bu sendromun başlıca iki nedeni, fertilizasyonun sekilenmemesi ve erken embriyonik ölümlerdir. Bu iki temel nedene yol açan ve RB sorununun ortaya çıkmasında rol oynayan birçok endokrinolojik faktör bulunmaktadır. Fizyolojik etkilerinden yola çıkılarak GnRH ve gonadotropinler RB'nin tedavisinde yaygın olarak kullanılmaktadır. Bu derlemede sütçü ineklerde RB sorununun nedenleri ile tedavisinde GnRH ve gonadotropinlerin kullanımı hakkında bilgi verilmiştir.
Turkish Journal of Veterinary & Animal Sciences, 2009
In order to study the prevalence and etiology of subclinical mastitis a bacteriological survey on... more In order to study the prevalence and etiology of subclinical mastitis a bacteriological survey on 16 Awassi dairy sheep flocks in southern Turkey was conducted. A total of 1458 milk samples from 729 Awassi ewes in mid-lactation were tested with the California mastitis test (CMT). Samples from 170 (11.7%) glands and 135 (18.5%) sheep had positive CMT results. Bacteria were isolated from 93 (6.4%) udder halves and 82 (11.2%) ewes. Positive CMT and bacteriological results were combined to define subclinical mastitis. The prevalence of subclinical mastitis and positive CMT samples among the different flocks ranged from 1.9% to 11.5% and 2.8% to 21.9% of the glands, and 3.8% to 19% and 5.7% to 31.3% of the ewes, respectively, with averages of 6.4% and 11.7% of the glands, and 11.2% and 18.5% of the ewes, respectively. Coagulase-negative staphylococci (CNS) were the most prevalent bacteria, representing 76.5% of the isolates. Staphylococcus epidermidis (35.7%) was the most prevalent species, followed by Staphylococcus xylosus (10.2%), Staphylococcus saprophyticus (10.2%), Staphylococcus warneri (9.2%), and Staphylococcus intermedius (7.1%). Antimicrobial susceptibility of 78 Staphylococcus isolates was evaluated in this study. The most effective antibiotics were cephalothin (97.4%), sulfamethoxazole-trimethoprim (97.4%), amoxicillin + clavulanic acid (97.4%), enrofloxacin (94.9%), gentamycin (92.3%), and erythromycin (84.6%). The relationship between CMT +1 score and the Staphylococcus spp. isolation rate was statistically significant (P < 0.001). It was concluded that subclinical mastitis is not highly prevalent and it does not pose a significant health problem for milking Awassi sheep flocks in southern Turkey.
Turkish Journal of Agriculture - Food Science and Technology
The purpose of this study was to determine the effects of selenium, vitamin E, vitamin A, and vit... more The purpose of this study was to determine the effects of selenium, vitamin E, vitamin A, and vitamin D3 treatments on reproductive parameters in Awassi sheep fed solely dry grass and grain stubble during the breeding season with estrus synchronization. Seventy-five sheep were implanted with intravaginal sponges containing 20 mg of flugestone acetate for 9 days for estrus synchronization. On the day the sponges were inserted, the first group received an intramuscular injection of a supplement containing 200,000 IU of vitamin A, 30,000 IU of vitamin D3, and 20 mg of vitamin E, as well as a supplement containing 1 mg of sodium selenite and 60 mg of vitamin E. The second group was the control group, with no supplementary vitamin injected. On the day of sponge removal, the sheep received intramuscular injections of 500 IU PMSG and 250 mcg cloprostenol sodium in both groups. The sheep in the first group were given a second injection of the supplement on the same day that contained 20 mg ...
Veterinary Journal of Mehmet Akif Ersoy University
The aim of this study was to evaluate the effect of selenium, β-carotene, and vitamin E administr... more The aim of this study was to evaluate the effect of selenium, β-carotene, and vitamin E administration on fertility of Awassi ewes synchronized in non-breeding season. A total of 80 multiparous Awassi ewes, aged 2 to 6 years were enrolled in the study. Intravaginal sponges containing flugeston acetate (20 mg cronolon) was inserted into vagina and allowed to remain in vagina for nine days. Ewes were randomly divided into two groups. Group I received intramuscular injections of 1 mg sodium selenite, 60 mg vitamin E, 75 mg β-carotene, and 100 mg DL-α-tocopherol acetate at sponge insertion, sponge removal, and 18 days after the introduction of ram. Group II remained as control group and received no treatment. On the removal of the sponges, 500 IU eCG and 0.075 mg D-cloprostenol were injected intramuscularly. Ram was introduced for 1 hr twice a day 24 hours after the sponge removal. Interval between sponge removal and estrus, estrus rates, conception rates, pregnancy rates, lambing rates...
Çalışma laktasyondaki ineklerde tohumlama anında uygulanan GnRH dozunun yarıya indirilmesinin geb... more Çalışma laktasyondaki ineklerde tohumlama anında uygulanan GnRH dozunun yarıya indirilmesinin gebelik oranı üzerine etkisini belirlemek amacıyla yapıldı. Araştırmada yaşları 3-6 arasında değişen, postpartum 80-120 günler arasında olan ve klinik olarak herhangi bir reprodüktif probleme rastlanılmayan 50 Holstayn ırkı inek kullanıldı. İneklerin östrüs göstermeleri için herhangi bir hormonal uygulama yapılmadı. Çalışma süresince östrüs belirtileri gösteren ineklerin ovaryum muayeneleri 6-8 MHz lineer problu real-time ultrason ile yapıldı ve ovaryumdaki mevcut foliküllerin çapı kaydedildi. Daha sonra bir gruba (grup I, n=25) suni tohumlama (ST) anında bir gonadotropin salgılatıcı hormon (GnRH) analoğu olan Buserelin asetat (Receptal®, İntervet) 2.5 mL kas içi uygulandı. Diğer gruba (grup II, n=25) ise ST anında 1.25 mL Buserelin asetat kas içi uygulandı. Folikül çapları grup I ve II'de sırasıyla 12.28±0.37 mm, 12.24±0.44 mm olarak kaydedildi. Gebelik oranları grup I ve II'de sır...
Sunulan çalışmada; koyunlarda embriyonik ölümlerin belirlenmesinde direkt bir yöntem olan real-ti... more Sunulan çalışmada; koyunlarda embriyonik ölümlerin belirlenmesinde direkt bir yöntem olan real-time ultrason ve indirekt bir yöntem olan plazma progesteron düzeyleri ile embriyonik ölümlerin insidansı belirlenmiş ve mevcut kayıplar değerlendirilmiştir. Çalışma materyalini en az bir doğum yapmış, 2-5 yaşlı, aynı bakim ve beslenme şartlarında barındırılan, flushing uygulanan, 17 Eylül-7 Ekim tarihleri arasında doğal aşım yaptırılan 60 gebe Konya Merinosu ırkı koyun oluşturdu. Koyunların ilk gebelik muayeneleri aşım sonrası 18. günde linear array prob ve 5 MHz frekansla transrektal yoldan yapıldı. Aşım sonrası 18. günde gebe olduğu belirlenen koyunlara izleyen 22, 26, 30 ve 34. günlerde de aynı muayene uygulandı. Ayrıca aynı muayene günlerinde (18, 22, 26, 30 ve 34. gün) plazma progesteron düzeyi enzyme immunoassay (EIA) yöntemiyle belirlendi. Onsekizinci günde yapılan ultrasonografik muayenede 73 koyundan 60'ında gebelik ve 79 embriyo belirlendi. Embriyoların 7 (% 8.9)'sinde e...
Sunulan çalışmada Konya Merinosu ırkı koyunlarda gebelik tanısı ve fötal sayım amacıyla geç embri... more Sunulan çalışmada Konya Merinosu ırkı koyunlarda gebelik tanısı ve fötal sayım amacıyla geç embriyonal ve erken fötal dönemde sağ (R) ve sol (L) inguinal bölgeden yapılan transabdominal ultrasonografik muayenenin (TAUM) etkinliğinin belirlenmesi amaçlandı. Bu amaçla aşım sonrası 34. günde transrektal ultrasonografik muayene (TRUM) ile 45 baş tek ve 39 baş ikiz embriyo taşıyan gebe koyun belirlendi. Toplam 84 baş gebe koyun 34 ve 50. günlerde, RTAUM ve LTAUM yoldan farklı saatlerde muayene edilerek, bulgular TRUM ve doğum kayıtları ile karşılaştırıldı. TRUM ve doğum kayıtlarına göre 34. günde tekli gebelik RTAUM ve LTAUM olarak sırasıyla % 62 (28/45) ve % 64 (29/45), 50. günde de sırasıyla % 88 (40/45) ve % 91 (41/45) oranında doğru olarak belirlendi. İkiz gebelikler ise RTAUM ve LTAUM olarak 34. günde sırasıyla % 33 (13/39) ve % 28 (11/39) oranında belirlenebilirken; 50. günde sırasıyla % 53 (21/39) ve % 46 (18/39) oranında doğru olarak belirlendi. Bulgulara göre gerek muayene günle...
Turkish Journal of Veterinary & Animal Sciences
For this study, 27 N. strauchii (16 males and 11 females) individuals were collected by hand and ... more For this study, 27 N. strauchii (16 males and 11 females) individuals were collected by hand and scoop net from a stream in Soğukpinar village (38°42′649″N, 40°27′091″E; altitude: 1073 m) in the Genç district of Bingöl Province in December 2010. Average body length of the newts ranged between 124 and 165 mm. Field collected samples were examined within a short duration (4 days) after the collection. Samples were injected with sodium pentobarbital and then opened from the ventral region. Body cavity and the inner organs were examined under stereo-binocular microscope for the presence of parasites. Recovered nematodes were transferred to warm, salty water for a while and then preserved in 70% alcoholic solutions for later examination. The nematodes were then mounted in gelatin-glycerol media as permanent slides and identified to the species level. The parasites and the newt samples were deposited in the
Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences, 2008
Iranian journal of veterinary research, 2021
Background Periostin (POSTN) is an extracellular matrix (ECM) protein that plays an important rol... more Background Periostin (POSTN) is an extracellular matrix (ECM) protein that plays an important role in the metastatic process and cancer cell migration. As implantation is a similar mechanism to metastasis, it has been hypothesized that POSTN may also play a role in the implantation process. Aims The aim of the present study was to compare POSTN and progesterone levels during the early pregnancy stage in Damascus goats. Methods Forty goats were synchronized using progesterone based sponges and were mated upon estrus signs display. While ten goats were kept as control (CON) and were not allowed to mate. Blood samples were taken through jugular venepuncture from CON and synchronized goats on day 13, 15, 17, 19, and 21 of breeding. Progesterone and POSTN levels were determined by enzyme-linked immunosorbent assay (ELISA). Later the pregnancy diagnosis was confirmed by transabdominal ultrasonography on day 50 after mating. Results Progesterone level was influenced by status of pregnancy ...
This research was carried out to determine the effect of Diclofenac sodium injections at 15-16th ... more This research was carried out to determine the effect of Diclofenac sodium injections at 15-16th days post mating on the pregnancy rate of the lactating goats. A total of 107 Damascus goats were synchronized by buck effect. The 54 goats between 3-6 years of age which were showing estrus and mated between the 9-15th days after the induction of buck to the herd constituted the material of the study. The goats were randomly assigned to two groups as diclofenac sodium (DFS) and control. Diclofenac sodium was injected to DFS group (n=27) intramuscularly with a dose of 2.5 mg/kg in the mornings of the 15-16th days postmating with 24 hours intervals twicely. Placebo was injected to control group at the same days and hours. The ultrasonographic examination performed at the 50th day post insemination revealed that while 77.8% (21/27) of the goats was determined to be pregnant in DFS group, the %51.9 (14/27) of the goats was found to be pregnant in control group. The pregnancy rates of the DF...
Repeat breeder sendromu (RB) sutcu ineklerde dogum-yeniden gebe kalma araligini olumsuz yonde etk... more Repeat breeder sendromu (RB) sutcu ineklerde dogum-yeniden gebe kalma araligini olumsuz yonde etkiledigi icin onemli bir sorundur. Bu sendromun baslica iki nedeni, fertilizasyonun sekilenmemesi ve erken embriyonik olumlerdir. Bu iki temel nedene yol acan ve RB sorununun ortaya cikmasinda rol oynayan bircok endokrinolojik faktor bulunmaktadir. Fizyolojik etkilerinden yola cikilarak GnRH ve gonadotropinler RB’nin tedavisinde yaygin olarak kullanilmaktadir. Bu derlemede sutcu ineklerde RB sorununun nedenleri ile tedavisinde GnRH ve gonadotropinlerin kullanimi hakkinda bilgi verilmistir.
Bulletin of The Veterinary Institute in Pulawy, 2009
The aim of the study was to evaluate the Epsilometer-test (E-test) method to detect the susceptib... more The aim of the study was to evaluate the Epsilometer-test (E-test) method to detect the susceptibility to amikacin and amoxicillin-clavulanic acid of 100 Staphylococcus species isolated from sheep and goats with subclinical mastitis. Of all the strains isolated, 24 were identified as Staphylococcus epidermidis, 17 as S. intermedius, 12 as S. xylosus, ten as S. warneri, nine as S. saprophyticus, nine as S. capitis, five as S. simulans, five as S. haemolyticus, three as S. sciuri, two as S. auricularis, two as S. hyicus, one as S. cohnii, and one as S. caprae. All isolates were sensitive to both antibiotics. While the MIC values of amoxicillin-clavulanic acid for the goat strains ranged from 0.125 to 1 µg/mL and of amikacin from 0.25 to 12 µg/mL, the MIC values for the sheep strains ranged from 0.047 to 2 µg/mL (amoxicillin-clavulanic acid) and 0.75 to 6 µg/ml (amikacin). The results of this study have shown that both antibiotics can be highly effective against Staphylococcus sp. that...
Calisma laktasyondaki ineklerde tohumlama aninda uygulanan GnRH dozunun yariya indirilmesinin geb... more Calisma laktasyondaki ineklerde tohumlama aninda uygulanan GnRH dozunun yariya indirilmesinin gebelik orani uzerine etkisini belirlemek amaciyla yapildi. Arastirmada yaslari 3-6 arasinda degisen, postpartum 80-120 gunler arasinda olan ve klinik olarak herhangi bir reproduktif probleme rastlanilmayan 50 Holstayn irki inek kullanildi. Ineklerin ostrus gostermeleri icin herhangi bir hormonal uygulama yapilmadi. Calisma suresince ostrus belirtileri gosteren ineklerin ovaryum muayeneleri 6-8 MHz lineer problu real-time ultrason ile yapildi ve ovaryumdaki mevcut folikullerin capi kaydedildi. Daha sonra bir gruba (grup I, n=25) suni tohumlama (ST) aninda bir gonadotropin salgilatici hormon ( GnRH) analogu olan Buserelin asetat (Receptal ® , Intervet) 2.5 mL kas ici uygulandi. Diger gruba (grup II, n=25) ise ST aninda 1.25 mL Buserelin asetat kas ici uygulandi. Folikul caplari grup I ve II’de sirasiyla 12.28±0.37 mm, 12.24±0.44 mm olarak kaydedildi. Gebelik oranlari grup I ve II’de sirasi...
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Papers by Mustafa Kemal sarıbay