Bioremediation Science and Technology Research, 2021
Microorganisms play an integral role in detoxification and removal of toxic compounds from the en... more Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to control weeds. Aniline is detrimental to both environment and health. In this research, six previously isolated bacteria (isolate A-F) were screened on Bushnell Hass media for their potential to grow and utilize aniline as a sole carbon source. Isolate A (Pseudomonas sp.) was found to tolerate and grow best with aniline sole source of carbon. Optimum conditions for aniline degradation by this isolate were found to be pH 6.0, temperature between 30 and 37 °C, inoculums size of 600 μL, aniline concentration of 200 mg/L and incubation time of 96 h. The capacity of this isolate to reduce toxic aniline to less toxic form is novel and makes the bacterium important instrument for bioremediation of this pollutant.
BIOREMEDIATION SCIENCE AND TECHNOLOGY RESEARCH, Dec 31, 2021
Microorganisms play an integral role in detoxification and removal of toxic compounds from the en... more Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to control weeds. Aniline is detrimental to both environment and health. In this research, six previously isolated bacteria (isolate A-F) were screened on Bushnell Hass media for their potential to grow and utilize aniline as a sole carbon source. Isolate A (Pseudomonas sp.) was found to tolerate and grow best with aniline sole source of carbon. Optimum conditions for aniline degradation by this isolate were found to be pH 6.0, temperature between 30 and 37 °C, inoculums size of 600 µL, aniline concentration of 200 mg/L and incubation time of 96 h. The capacity of this isolate to reduce toxic aniline to less toxic form is novel and makes the bacterium important instrument for bioremediation of this pollutant.
Journal of Environmental Microbiology and Toxicology
Bioremediation of pollutants, such as herbicides, is an economic and environmentally friendly pro... more Bioremediation of pollutants, such as herbicides, is an economic and environmentally friendly process. Glyphosate is an active ingredient in most herbicides utilized for weed control and desiccation on cereal and other grain crops globally. Glyphosate pollution poses a threat to the environment and the habitats in it. In this study, an isolated molybdenum-reducing bacterium was characterized for its potential to degrade glyphosate and utilized as the sole source of carbon and electron donor. The effects of incubation time, glyphosate concentration (carbon source), inoculum size, pH, temperature, aeration and heavy metals on the growth of this bacterium were spectrophotometrically assayed as OD600 nm. The bacterium degrades glyphosate faster under shaking conditions, optimally at pH 7.0, concentration 1.0 g/L, temperature 40 ºC, and inoculum size 400 µL. Growth of this bacterium was significantly inhibited by heavy metals in the order of Cu>Zn>Pb>Hg>Ag>Fe compared to t...
Pollution of the environment by heavy metals and other toxic xenobiotics has increasingly become ... more Pollution of the environment by heavy metals and other toxic xenobiotics has increasingly become global public health concern. Bacterial reduction of molybdenum to insoluble molybdenum blue (Mo-blue) forms the basis for its bioremediation. A bacterium with the ability to reduce toxic soluble molybdenum has been isolated from Agricultural soil and identified as Pseudomonas sp. based on the 16S rRNA partial sequencing and phylogenetic analysis. Spectroscopic analysis reveals that the bacterium reduced sodium molybdate to Mo-blue optimally at pH between 6.5 and 7.0, temperatures between 35 °C and 40 °C. Glucose was the best electron donor source supporting molybdate reduction, followed by sucrose, fructose, glycerol and starch in descending order. Other requirements include a phosphate concentration of 3.5 mM and a molybdate concentration of between 40 and 60 mM. The absorption spectrum of the Mo-blue produced was similar to the previously isolated Mo-reducing bacteria and closely re...
Journal of Environmental Microbiology and Toxicology, 2019
Agricultural and industrial activities contribute most to the pollutants found globally; therefor... more Agricultural and industrial activities contribute most to the pollutants found globally; therefore, bioremediation of these pollutants is intensely sought. This research isolated a molybdenum-reducing bacterium from agricultural soil. The bacterium grown in low phosphate media (LPM) reduces molybadate to Mo-blue optimally at pH between 6.5 and 7.0, temperature between 35 and 40 °C, glucose at 5 g/L and glycine at 3 g/L were the best electron donor and nitrogen sources, respectively. The optimum molybdate concentration is between 80 and 100 mM, and phosphate concentration was between 5.0 and 7.5 mM. Phylogenetic analysis of 16S rRNA partial sequencing identified the bacterium as Enterobacter cloacae. The capacity of this bacterium to reduce toxic molybdenum to less toxic colloidal molybdenum blue is novel and form the basis for its use in future bioremediation of this pollutant.
Bioremediation Science and Technology Research, 2019
Intensive agriculture and industrial activities have significantly increased the global burden of... more Intensive agriculture and industrial activities have significantly increased the global burden of pollutants; thus, bioremediation of these pollutants is intensely sought. A bacterium with potential of reducing toxic soluble molybdenum to precipitable molybdenum blue (Mo-blue) was isolated from agricultural soil in Darmanawa, Kano state. The bacterium grown on low phosphate media (LPM) reduces molybadate to Mo-blue optimally at pH between 6.0 and 7.5, temperature of 35 °C, glucose was the best electron donor source at 5 g/L and ammonium sulphate was the best nitrogen source. The optimum molybdate concentration supporting the reduction process was 40 mM at 3.5 mM phosphate. Phylogenetic analysis of 16S rRNA partial sequence identified the bacterium as Morganella sp. The ability of this isolate to reduce toxic soluble molybdenum to colloidal less toxic form is novel and makes the bacterium an important instrument for bioremediation of this pollutant.
One of the beneficial roles of the microbial population is their ability to convert toxic herbici... more One of the beneficial roles of the microbial population is their ability to convert toxic herbicides to lesser toxic compounds such as water and carbon (IV) oxide. Paraquat which is an acutely toxic herbicide is used on farmlands and has been found to affect human health. This study was aimed at characterizing bacteria with the potential to degrade paraquat. Previously isolated bacteria from culture collection labelled A-F were screened for their potential to degrade and utilized paraquat as the sole carbon source in Bushnell Hass agar media. Of the six isolates, isolate E (Morganella sp.) was observed to have the highest growth and tolerance to paraquat after 72 h of incubation at 37 ºC. Characterization study revealed that Morganella sp. can utilize and grow with optimum conditions of pH 6.5, the temperature of 30 ºC and can tolerate up to 400 mg/L paraquat concentration with an increase in growth as inoculum size increases. Thus, these findings showed that Morganella sp. can degr...
Nigerian Journal of Basic and Clinical Sciences, 2017
Background: Changes in blood cell profile were common findings in malaria. In the rural community... more Background: Changes in blood cell profile were common findings in malaria. In the rural community of Kano State, Nigeria, information on haematological changes in human malaria was scanty in spite of their role in the pathophysiology of malaria. This cross-sectional study was undertaken to determine blood cell profiles in malaria patients attending a rural hospital in malariaendemic region. Methods: Blood samples (3 ml each) were collected in EDTA-containers from 150 randomly selected outpatients attending Gaya General Hospital, screened for malaria using RDT kit (CareStart Malaria HRP 2, Access Bio Inc., USA) based on Histidine-rich protein 2 (PfHRP-2), and blood cell profiles determined using automated Sysmex haematologic analyser. Data on sociodemographics and medical history related to the study objectives, such as taking antimalarial regimen and/or haematinic, and direct involvement in blood transfusion, were obtained by questionnaire administration supplemented with oral interview.
Journal of Biochemistry, Microbiology and Biotechnology, 2019
Bioremediation of pollutants such as heavy metals is an economic and environmentally friendly pro... more Bioremediation of pollutants such as heavy metals is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium was isolated and characterized for its potential to reduce hexavalent molybdenum to molybdenum blue (Mo-blue). The bacterium reduces molybdate optimally at pH between 6 and 8, temperature between 35 and 40 ËšC. Glucose was the best electron donor source supporting molybdate reduction followed by sucrose, fructose, starch and glycerol in descending order. Other requirements include optimum phosphate concentration at 5.0 mM and molybdate concentration between 20 and 40 mM. 16S rRNA partial sequencing and phylogenetic analysis identified the bacterium as Pantoea sp. strain HMY-P4. The capacity of this bacterium to reduce toxic soluble molybdenum to a less toxic form is novel and makes the bacterium an important instrument for bioremediation of this pollutant.
Journal of Environmental Bioremediation and Toxicology
Lots of studies do not perform statistical diagnostics on the nonlinear model that was used, ther... more Lots of studies do not perform statistical diagnostics on the nonlinear model that was used, therefore the data may not be random. Because these systems rely on random data, this is a necessity for all parametric statistical assessment procedures. When the diagnostic tests show that the residuals constitute a pattern, there are several options for treatment, including switching to a new model or performing a nonparametric analysis. These treatments, as well as others like them, should solve the issue. We employ the Wald-Wolfowitz runs test as a statistical diagnosis tool to determine whether or not the randomization conditions have been met. Because it was critical to examine the randomness of the residual for the Buchanan-3-phase model used in the fitting of the effect of nZVI/Pd and SiO2-nZVI/Pd nanoparticles on the growth of Pseudomonas putida, it was decided to conduct this study using the Wald-Wolfowitz runs test. The run test indicated that there were 7 total runs, whereas 7.1...
Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353)
Hypertension and kidney damage have been linked to lead exposure in adults. Any person can be har... more Hypertension and kidney damage have been linked to lead exposure in adults. Any person can be harmed by lead, but pregnant women and their unborn children are especially vulnerable. Presently, lead pollution is removed using membrane separation, ion exchange, precipitation, and biosorption. Biosorption's low operating costs, high efficiency at detoxifying low concentrations of toxicants, and small volume of disposal materials make it the least problematic of these technologies. The biosorption of lead (II) onto activated carbon from Tridax procumbens is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Freundlich model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc) The calculated Freundlich parameters kF value using nonlinear regression was 1.334...
There is currently no feasible method of recycling Cd compounds, despite the fact that Cd product... more There is currently no feasible method of recycling Cd compounds, despite the fact that Cd production, consumption, and environmental release have all skyrocketed in recent decades. This raises serious concerns about the potential dangers of Cd compounds to human health. This highlights the pressing need for cadmium pollution cleanup. Biosorption is one of several viable technologies with several advantages, including low operating costs, very efficient detoxification of toxicants at low concentrations, and a low amount of disposal materials. The biosorption of cadmium onto activated carbon from Tridax procumbens is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Freundlich model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc). This is in contrast to...
Journal of Environmental Microbiology and Toxicology
The biosorption of the biosorption of lead(II) onto an Antarctic sea-ice bacterial exopolysacchar... more The biosorption of the biosorption of lead(II) onto an Antarctic sea-ice bacterial exopolysaccharide is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Sips model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc) which is not the same to the originally published work using a linearized form where the Langmuir and Freundlich models best represent the biosorption and the maximum biosorption capacity. The calculated Sips parameters kS (l/g) value of 0.10 (95% confidence interval from 0.08 to 0.13), a maximum monolayer adsorption capacity qmS (mg/g) value of 252.88 (95% C.I. from 222.13 to 283.64) and nS (Sips model exponent) value of 1.16 (95% C.I. from 1.34 to 1.98). This study indicates that a different isotherm model can be obtained using nonlinear re...
Malaysian Journal of Applied Sciences, Dec 31, 2019
A cytosolic glutathione S-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl de... more A cytosolic glutathione S-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl degrading organism is recently known to have a dehalogenation function on various organochlorine substrates. However, little is known about the specific amino acids involved in this catalytic process. The in silico sitedirected mutagenesis of a highly conserved region; Ala 154 , Asp 155 and Tyr 157 in the C-terminal domain of the cytosolic glutathione S-transferase from Acidovorax sp. was carried out using Deep View/Swiss-Pdb Viewer molecular graphics program for all the proteinogenic amino acids. Sustitutions at position 154 of Ala for Phe, Trp and Tyr showed tendency of greater effect on the protein. At position 155, substitution of Asp for His, Phe, Trp and Tyr have greater effect on the 3D model of the protein. For substitution of Tyr at position 157, only Pro substitution showed greater effect on the protein model. The amino acid substitutions in this region may likely affect the theoretical 3D model of the transferase protein entity through alterations of some stabilization forces which may in turn affect the structural stability and perhaps the activity of the enzyme.
A cytosolic glutathione s-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl de... more A cytosolic glutathione s-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl degrading organism is recently known to have a dehalogenation function on various organochlorine substrates. However, little is known about the specific amino acids involved in its structural stability and catalytic process. The in silico site-directed mutagenesis of a highly conserved region, Ala154, Asp155 and Tyr157 in the C-terminal domain of the cytosolic glutathione s-transferase from Acidovorax sp. was carried out using Deep View/Swiss-Pdb Viewer molecular graphics program for all the proteinogenic amino acids. The amino acid substitutions in this region directly affected the theoretical 3D model of the transferase protein entity through alteration of the predicted stabilization forces which may in turn affect the structural stability and perhaps the activity of the enzyme
The increasing prices of calf rennets, their accessibility and ethical concerns associated with t... more The increasing prices of calf rennets, their accessibility and ethical concerns associated with the production of such enzymes for general cheese making have led to systematic investigations on the possibility and suitability of their substitution by other enzymes of plant origin. In this study, ammonium sulphate ((NH 4 ) 2 SO 4 ) fractionation, characterization and milk clotting activity (MCA) of protease extracted from Taffin Giwa ginger rhizome cultivar of the family Zingiberaceae from northwestern Nigeria were carried out. The protease extracted showed optimum activity at temperatures near 60 °C and pH value of 6.5 with a relative activity in a broad pH range of 5.0 to 8.0 accordingly. The enzyme was completely denatured at higher temperature of 100 °C and higher pH range of 12.0. The milk clotting property of the protease indicated 3.1 and 2.2 folds of MCA and MSCA respectively in relation to the commercial calf rennet with MCA/PA ratio of 2.52. The properties of Taffin Giwa pr...
In this study, twenty seven (27) female albino rats were used in the determination of contracepti... more In this study, twenty seven (27) female albino rats were used in the determination of contraceptive effect of oral administration of aqueous suspension of Ricinus communis seed. Estrogen and progesterone levels of these rats were analysed using electrochemiluminescent method. The result revealed estrogenic property of the aqueous suspension of R. communis seed, which is partly attributed to the anticonceptive effect of the seeds. The estrogenic activity was high with 107×10-2 mg/kg dose administered after 48hours and 7 days respectively, indicating some degree of dose-dependency. In addition, alteration of estrogen/progesterone balance as indicated by this study may be responsible for the contraceptive effect of aqueous suspension of R. communis seed.
Bioremediation Science and Technology Research, 2021
Microorganisms play an integral role in detoxification and removal of toxic compounds from the en... more Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to control weeds. Aniline is detrimental to both environment and health. In this research, six previously isolated bacteria (isolate A-F) were screened on Bushnell Hass media for their potential to grow and utilize aniline as a sole carbon source. Isolate A (Pseudomonas sp.) was found to tolerate and grow best with aniline sole source of carbon. Optimum conditions for aniline degradation by this isolate were found to be pH 6.0, temperature between 30 and 37 °C, inoculums size of 600 μL, aniline concentration of 200 mg/L and incubation time of 96 h. The capacity of this isolate to reduce toxic aniline to less toxic form is novel and makes the bacterium important instrument for bioremediation of this pollutant.
BIOREMEDIATION SCIENCE AND TECHNOLOGY RESEARCH, Dec 31, 2021
Microorganisms play an integral role in detoxification and removal of toxic compounds from the en... more Microorganisms play an integral role in detoxification and removal of toxic compounds from the environment. Aniline is the simplest aromatic amine, consisting of a phenyl group attached to an amino group that is used as herbicide to control weeds. Aniline is detrimental to both environment and health. In this research, six previously isolated bacteria (isolate A-F) were screened on Bushnell Hass media for their potential to grow and utilize aniline as a sole carbon source. Isolate A (Pseudomonas sp.) was found to tolerate and grow best with aniline sole source of carbon. Optimum conditions for aniline degradation by this isolate were found to be pH 6.0, temperature between 30 and 37 °C, inoculums size of 600 µL, aniline concentration of 200 mg/L and incubation time of 96 h. The capacity of this isolate to reduce toxic aniline to less toxic form is novel and makes the bacterium important instrument for bioremediation of this pollutant.
Journal of Environmental Microbiology and Toxicology
Bioremediation of pollutants, such as herbicides, is an economic and environmentally friendly pro... more Bioremediation of pollutants, such as herbicides, is an economic and environmentally friendly process. Glyphosate is an active ingredient in most herbicides utilized for weed control and desiccation on cereal and other grain crops globally. Glyphosate pollution poses a threat to the environment and the habitats in it. In this study, an isolated molybdenum-reducing bacterium was characterized for its potential to degrade glyphosate and utilized as the sole source of carbon and electron donor. The effects of incubation time, glyphosate concentration (carbon source), inoculum size, pH, temperature, aeration and heavy metals on the growth of this bacterium were spectrophotometrically assayed as OD600 nm. The bacterium degrades glyphosate faster under shaking conditions, optimally at pH 7.0, concentration 1.0 g/L, temperature 40 ºC, and inoculum size 400 µL. Growth of this bacterium was significantly inhibited by heavy metals in the order of Cu>Zn>Pb>Hg>Ag>Fe compared to t...
Pollution of the environment by heavy metals and other toxic xenobiotics has increasingly become ... more Pollution of the environment by heavy metals and other toxic xenobiotics has increasingly become global public health concern. Bacterial reduction of molybdenum to insoluble molybdenum blue (Mo-blue) forms the basis for its bioremediation. A bacterium with the ability to reduce toxic soluble molybdenum has been isolated from Agricultural soil and identified as Pseudomonas sp. based on the 16S rRNA partial sequencing and phylogenetic analysis. Spectroscopic analysis reveals that the bacterium reduced sodium molybdate to Mo-blue optimally at pH between 6.5 and 7.0, temperatures between 35 °C and 40 °C. Glucose was the best electron donor source supporting molybdate reduction, followed by sucrose, fructose, glycerol and starch in descending order. Other requirements include a phosphate concentration of 3.5 mM and a molybdate concentration of between 40 and 60 mM. The absorption spectrum of the Mo-blue produced was similar to the previously isolated Mo-reducing bacteria and closely re...
Journal of Environmental Microbiology and Toxicology, 2019
Agricultural and industrial activities contribute most to the pollutants found globally; therefor... more Agricultural and industrial activities contribute most to the pollutants found globally; therefore, bioremediation of these pollutants is intensely sought. This research isolated a molybdenum-reducing bacterium from agricultural soil. The bacterium grown in low phosphate media (LPM) reduces molybadate to Mo-blue optimally at pH between 6.5 and 7.0, temperature between 35 and 40 °C, glucose at 5 g/L and glycine at 3 g/L were the best electron donor and nitrogen sources, respectively. The optimum molybdate concentration is between 80 and 100 mM, and phosphate concentration was between 5.0 and 7.5 mM. Phylogenetic analysis of 16S rRNA partial sequencing identified the bacterium as Enterobacter cloacae. The capacity of this bacterium to reduce toxic molybdenum to less toxic colloidal molybdenum blue is novel and form the basis for its use in future bioremediation of this pollutant.
Bioremediation Science and Technology Research, 2019
Intensive agriculture and industrial activities have significantly increased the global burden of... more Intensive agriculture and industrial activities have significantly increased the global burden of pollutants; thus, bioremediation of these pollutants is intensely sought. A bacterium with potential of reducing toxic soluble molybdenum to precipitable molybdenum blue (Mo-blue) was isolated from agricultural soil in Darmanawa, Kano state. The bacterium grown on low phosphate media (LPM) reduces molybadate to Mo-blue optimally at pH between 6.0 and 7.5, temperature of 35 °C, glucose was the best electron donor source at 5 g/L and ammonium sulphate was the best nitrogen source. The optimum molybdate concentration supporting the reduction process was 40 mM at 3.5 mM phosphate. Phylogenetic analysis of 16S rRNA partial sequence identified the bacterium as Morganella sp. The ability of this isolate to reduce toxic soluble molybdenum to colloidal less toxic form is novel and makes the bacterium an important instrument for bioremediation of this pollutant.
One of the beneficial roles of the microbial population is their ability to convert toxic herbici... more One of the beneficial roles of the microbial population is their ability to convert toxic herbicides to lesser toxic compounds such as water and carbon (IV) oxide. Paraquat which is an acutely toxic herbicide is used on farmlands and has been found to affect human health. This study was aimed at characterizing bacteria with the potential to degrade paraquat. Previously isolated bacteria from culture collection labelled A-F were screened for their potential to degrade and utilized paraquat as the sole carbon source in Bushnell Hass agar media. Of the six isolates, isolate E (Morganella sp.) was observed to have the highest growth and tolerance to paraquat after 72 h of incubation at 37 ºC. Characterization study revealed that Morganella sp. can utilize and grow with optimum conditions of pH 6.5, the temperature of 30 ºC and can tolerate up to 400 mg/L paraquat concentration with an increase in growth as inoculum size increases. Thus, these findings showed that Morganella sp. can degr...
Nigerian Journal of Basic and Clinical Sciences, 2017
Background: Changes in blood cell profile were common findings in malaria. In the rural community... more Background: Changes in blood cell profile were common findings in malaria. In the rural community of Kano State, Nigeria, information on haematological changes in human malaria was scanty in spite of their role in the pathophysiology of malaria. This cross-sectional study was undertaken to determine blood cell profiles in malaria patients attending a rural hospital in malariaendemic region. Methods: Blood samples (3 ml each) were collected in EDTA-containers from 150 randomly selected outpatients attending Gaya General Hospital, screened for malaria using RDT kit (CareStart Malaria HRP 2, Access Bio Inc., USA) based on Histidine-rich protein 2 (PfHRP-2), and blood cell profiles determined using automated Sysmex haematologic analyser. Data on sociodemographics and medical history related to the study objectives, such as taking antimalarial regimen and/or haematinic, and direct involvement in blood transfusion, were obtained by questionnaire administration supplemented with oral interview.
Journal of Biochemistry, Microbiology and Biotechnology, 2019
Bioremediation of pollutants such as heavy metals is an economic and environmentally friendly pro... more Bioremediation of pollutants such as heavy metals is an economic and environmentally friendly process. A novel molybdenum-reducing bacterium was isolated and characterized for its potential to reduce hexavalent molybdenum to molybdenum blue (Mo-blue). The bacterium reduces molybdate optimally at pH between 6 and 8, temperature between 35 and 40 ËšC. Glucose was the best electron donor source supporting molybdate reduction followed by sucrose, fructose, starch and glycerol in descending order. Other requirements include optimum phosphate concentration at 5.0 mM and molybdate concentration between 20 and 40 mM. 16S rRNA partial sequencing and phylogenetic analysis identified the bacterium as Pantoea sp. strain HMY-P4. The capacity of this bacterium to reduce toxic soluble molybdenum to a less toxic form is novel and makes the bacterium an important instrument for bioremediation of this pollutant.
Journal of Environmental Bioremediation and Toxicology
Lots of studies do not perform statistical diagnostics on the nonlinear model that was used, ther... more Lots of studies do not perform statistical diagnostics on the nonlinear model that was used, therefore the data may not be random. Because these systems rely on random data, this is a necessity for all parametric statistical assessment procedures. When the diagnostic tests show that the residuals constitute a pattern, there are several options for treatment, including switching to a new model or performing a nonparametric analysis. These treatments, as well as others like them, should solve the issue. We employ the Wald-Wolfowitz runs test as a statistical diagnosis tool to determine whether or not the randomization conditions have been met. Because it was critical to examine the randomness of the residual for the Buchanan-3-phase model used in the fitting of the effect of nZVI/Pd and SiO2-nZVI/Pd nanoparticles on the growth of Pseudomonas putida, it was decided to conduct this study using the Wald-Wolfowitz runs test. The run test indicated that there were 7 total runs, whereas 7.1...
Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353)
Hypertension and kidney damage have been linked to lead exposure in adults. Any person can be har... more Hypertension and kidney damage have been linked to lead exposure in adults. Any person can be harmed by lead, but pregnant women and their unborn children are especially vulnerable. Presently, lead pollution is removed using membrane separation, ion exchange, precipitation, and biosorption. Biosorption's low operating costs, high efficiency at detoxifying low concentrations of toxicants, and small volume of disposal materials make it the least problematic of these technologies. The biosorption of lead (II) onto activated carbon from Tridax procumbens is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Freundlich model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc) The calculated Freundlich parameters kF value using nonlinear regression was 1.334...
There is currently no feasible method of recycling Cd compounds, despite the fact that Cd product... more There is currently no feasible method of recycling Cd compounds, despite the fact that Cd production, consumption, and environmental release have all skyrocketed in recent decades. This raises serious concerns about the potential dangers of Cd compounds to human health. This highlights the pressing need for cadmium pollution cleanup. Biosorption is one of several viable technologies with several advantages, including low operating costs, very efficient detoxification of toxicants at low concentrations, and a low amount of disposal materials. The biosorption of cadmium onto activated carbon from Tridax procumbens is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Freundlich model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc). This is in contrast to...
Journal of Environmental Microbiology and Toxicology
The biosorption of the biosorption of lead(II) onto an Antarctic sea-ice bacterial exopolysacchar... more The biosorption of the biosorption of lead(II) onto an Antarctic sea-ice bacterial exopolysaccharide is remodeled using nonlinear regression and the optimal mode was determined by a series of error function assessments. The Sips model performed best in statistical tests including root-mean-square error (RMSE), adjusted coefficient of determination (adjR2), bias factor (BF), accuracy factor (AF), and corrected Akaike Information Criterion (AICc) which is not the same to the originally published work using a linearized form where the Langmuir and Freundlich models best represent the biosorption and the maximum biosorption capacity. The calculated Sips parameters kS (l/g) value of 0.10 (95% confidence interval from 0.08 to 0.13), a maximum monolayer adsorption capacity qmS (mg/g) value of 252.88 (95% C.I. from 222.13 to 283.64) and nS (Sips model exponent) value of 1.16 (95% C.I. from 1.34 to 1.98). This study indicates that a different isotherm model can be obtained using nonlinear re...
Malaysian Journal of Applied Sciences, Dec 31, 2019
A cytosolic glutathione S-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl de... more A cytosolic glutathione S-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl degrading organism is recently known to have a dehalogenation function on various organochlorine substrates. However, little is known about the specific amino acids involved in this catalytic process. The in silico sitedirected mutagenesis of a highly conserved region; Ala 154 , Asp 155 and Tyr 157 in the C-terminal domain of the cytosolic glutathione S-transferase from Acidovorax sp. was carried out using Deep View/Swiss-Pdb Viewer molecular graphics program for all the proteinogenic amino acids. Sustitutions at position 154 of Ala for Phe, Trp and Tyr showed tendency of greater effect on the protein. At position 155, substitution of Asp for His, Phe, Trp and Tyr have greater effect on the 3D model of the protein. For substitution of Tyr at position 157, only Pro substitution showed greater effect on the protein model. The amino acid substitutions in this region may likely affect the theoretical 3D model of the transferase protein entity through alterations of some stabilization forces which may in turn affect the structural stability and perhaps the activity of the enzyme.
A cytosolic glutathione s-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl de... more A cytosolic glutathione s-tranferase from Acidovorax sp. KKS102, a biphenyl/polychlorobiphenyl degrading organism is recently known to have a dehalogenation function on various organochlorine substrates. However, little is known about the specific amino acids involved in its structural stability and catalytic process. The in silico site-directed mutagenesis of a highly conserved region, Ala154, Asp155 and Tyr157 in the C-terminal domain of the cytosolic glutathione s-transferase from Acidovorax sp. was carried out using Deep View/Swiss-Pdb Viewer molecular graphics program for all the proteinogenic amino acids. The amino acid substitutions in this region directly affected the theoretical 3D model of the transferase protein entity through alteration of the predicted stabilization forces which may in turn affect the structural stability and perhaps the activity of the enzyme
The increasing prices of calf rennets, their accessibility and ethical concerns associated with t... more The increasing prices of calf rennets, their accessibility and ethical concerns associated with the production of such enzymes for general cheese making have led to systematic investigations on the possibility and suitability of their substitution by other enzymes of plant origin. In this study, ammonium sulphate ((NH 4 ) 2 SO 4 ) fractionation, characterization and milk clotting activity (MCA) of protease extracted from Taffin Giwa ginger rhizome cultivar of the family Zingiberaceae from northwestern Nigeria were carried out. The protease extracted showed optimum activity at temperatures near 60 °C and pH value of 6.5 with a relative activity in a broad pH range of 5.0 to 8.0 accordingly. The enzyme was completely denatured at higher temperature of 100 °C and higher pH range of 12.0. The milk clotting property of the protease indicated 3.1 and 2.2 folds of MCA and MSCA respectively in relation to the commercial calf rennet with MCA/PA ratio of 2.52. The properties of Taffin Giwa pr...
In this study, twenty seven (27) female albino rats were used in the determination of contracepti... more In this study, twenty seven (27) female albino rats were used in the determination of contraceptive effect of oral administration of aqueous suspension of Ricinus communis seed. Estrogen and progesterone levels of these rats were analysed using electrochemiluminescent method. The result revealed estrogenic property of the aqueous suspension of R. communis seed, which is partly attributed to the anticonceptive effect of the seeds. The estrogenic activity was high with 107×10-2 mg/kg dose administered after 48hours and 7 days respectively, indicating some degree of dose-dependency. In addition, alteration of estrogen/progesterone balance as indicated by this study may be responsible for the contraceptive effect of aqueous suspension of R. communis seed.
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