DETEKSI PENYAKIT TILV (Tilapia Lake Virus) PADA IKAN NILA (Oreochromis niloticus) DENGAN METODE RT-PCR DI BALAI KIPM MEDAN I, 2021
Lately in some areas there have been cases of death in the cultivation of tilapia fish en masse w... more Lately in some areas there have been cases of death in the cultivation of tilapia fish en masse which is likely to be an infection of TiLV disease. TILV that attacks tilapia can be quick diagnosed with RT - Polymerase Chain Reaction (RT-PCR) method. This examination method detect the virus through the presence of its RNA. The purpose of this study is to determine the effectiveness of RT-PCR method in detecting disease in tilapia (Oreochromis niloticus) at the Fish Quarantine Station for Quality Control and Safety of Fishery Products in Medan I (BKIPM Medan I). Qualitative research method was conducted in BKIPM Medan I. Tilapia sample were taken from tilapia pond in Porsea and Laguboti, Toba Samosir, North Sumatera. Fifteen tilapias were taken from each pond. The sample criteria was 30 days old tilapia or tilapia that shown clinical symptom. RT-PCR method was perform at sample brain, kidney, and liver. The result from RT-PCR than analyzed and compared with the literature. The results of the study using the RT-PCR method on samples of tilapia (Oreochromis niloticus) from Porsea sub-district showed positive infected with TILV with the results of electrophoresis rna band TiLV appeared at 250 bp and the percentage of infestation of 66.67%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. So it can be oncluded that RT-PCR effectively detects TILV disease in tilapia fish.
Masalah dalam penelitian ini dirumusakan adalah Apakah terdapat Pengaruh antara Penggunaan Model ... more Masalah dalam penelitian ini dirumusakan adalah Apakah terdapat Pengaruh antara Penggunaan Model Pembelajaran Kooperatif Tipe Two Stay Two Stray (TSTS) Terhadap Hasil Belajar Pada Pelajaran IPS Materi Pokok Kriminalitas di Kelas IV SD Negeri 102115 Gunung Pamela Kec. Sipispis Kab. Serdang Bedagai TA 2013/2014?. Penelitian ini bertujuan untuk mengetahui Pengaruh Penggunaan Model Pembelajaran Kooperatif Tipe Two Stay Two Stray (TSTS) Terhadap Hasil Belajar Pada Pelajaran IPS Materi Pokok Kriminalitas di Kelas IV SD Negeri 102115 Gunung Pamela Kec. Sipispis Kab. Serdang Bedagai TA 2013/2014.Jenis penelitian ini adalah penelitian populasi. Untuk pengetahuan tersebut data diambil dari sampel yaitu kelas IV yang masing-masing terdiri dua kelas sebanyak 72 Siswa. Sampel diambil dari populasi yang terdiri dari dua kelas. Metode yang digunakan dalam penelitian ini adalah metode eksperimen semu. Data diambil dengan menggunakan analisis statistik deskriptif dan uji t.Hasil perhitungan pada pen...
Akhir-akhir ini di beberapa daerah terjadi kasus kematian pada budidaya ikan nila secara massal y... more Akhir-akhir ini di beberapa daerah terjadi kasus kematian pada budidaya ikan nila secara massal yang kemungkinan infeksi penyakit TiLV. Diagnosa TILV yang menyerang ikan nila dapat dilakukan secara dini menggunakan metode Polymerase Chain Reaction (PCR) secara konvensional sehingga dapat diambil tindakan pencegahan khususnya pada benur ikan nila yang akan ditebar. Metode pemeriksaan tersebut mampu mendeteksi virus melalui keberadaan RNA virus. Tujuan penelitian adalah mengetahui efektivitas metode RT-PCR dalam mendeteksi penyakit pada ikan nila (Oreochromis niloticus) di BKIPM Medan I. Metode penelitian menggunakan metode eksperimen bersifat kualitatif dilaksanakan di Laboratorium Biomolekuler di Balai Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan (BKIPM Medan I). Sampel ikan nila berasal dari tambak ikan nila di Kecamatan Porsea dan Laguboti, Toba Samosir, Sumatera Utara yang terdiri atas masing-masing 15 ekor ikan nila berumur 30 hari atau ikan nila yang sudah meng...
DETEKSI PENYAKIT TILV (Tilapia Lake Virus) PADA IKAN NILA (Oreochromis niloticus) DENGAN METODE RT-PCR DI BALAI KIPM MEDAN I, 2021
Lately in some areas there have been cases of death in the cultivation of tilapia fish en masse w... more Lately in some areas there have been cases of death in the cultivation of tilapia fish en masse which is likely to be an infection of TiLV disease. TILV that attacks tilapia can be quick diagnosed with RT - Polymerase Chain Reaction (RT-PCR) method. This examination method detect the virus through the presence of its RNA. The purpose of this study is to determine the effectiveness of RT-PCR method in detecting disease in tilapia (Oreochromis niloticus) at the Fish Quarantine Station for Quality Control and Safety of Fishery Products in Medan I (BKIPM Medan I). Qualitative research method was conducted in BKIPM Medan I. Tilapia sample were taken from tilapia pond in Porsea and Laguboti, Toba Samosir, North Sumatera. Fifteen tilapias were taken from each pond. The sample criteria was 30 days old tilapia or tilapia that shown clinical symptom. RT-PCR method was perform at sample brain, kidney, and liver. The result from RT-PCR than analyzed and compared with the literature. The results of the study using the RT-PCR method on samples of tilapia (Oreochromis niloticus) from Porsea sub-district showed positive infected with TILV with the results of electrophoresis rna band TiLV appeared at 250 bp and the percentage of infestation of 66.67%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. So it can be oncluded that RT-PCR effectively detects TILV disease in tilapia fish.
Masalah dalam penelitian ini dirumusakan adalah Apakah terdapat Pengaruh antara Penggunaan Model ... more Masalah dalam penelitian ini dirumusakan adalah Apakah terdapat Pengaruh antara Penggunaan Model Pembelajaran Kooperatif Tipe Two Stay Two Stray (TSTS) Terhadap Hasil Belajar Pada Pelajaran IPS Materi Pokok Kriminalitas di Kelas IV SD Negeri 102115 Gunung Pamela Kec. Sipispis Kab. Serdang Bedagai TA 2013/2014?. Penelitian ini bertujuan untuk mengetahui Pengaruh Penggunaan Model Pembelajaran Kooperatif Tipe Two Stay Two Stray (TSTS) Terhadap Hasil Belajar Pada Pelajaran IPS Materi Pokok Kriminalitas di Kelas IV SD Negeri 102115 Gunung Pamela Kec. Sipispis Kab. Serdang Bedagai TA 2013/2014.Jenis penelitian ini adalah penelitian populasi. Untuk pengetahuan tersebut data diambil dari sampel yaitu kelas IV yang masing-masing terdiri dua kelas sebanyak 72 Siswa. Sampel diambil dari populasi yang terdiri dari dua kelas. Metode yang digunakan dalam penelitian ini adalah metode eksperimen semu. Data diambil dengan menggunakan analisis statistik deskriptif dan uji t.Hasil perhitungan pada pen...
Akhir-akhir ini di beberapa daerah terjadi kasus kematian pada budidaya ikan nila secara massal y... more Akhir-akhir ini di beberapa daerah terjadi kasus kematian pada budidaya ikan nila secara massal yang kemungkinan infeksi penyakit TiLV. Diagnosa TILV yang menyerang ikan nila dapat dilakukan secara dini menggunakan metode Polymerase Chain Reaction (PCR) secara konvensional sehingga dapat diambil tindakan pencegahan khususnya pada benur ikan nila yang akan ditebar. Metode pemeriksaan tersebut mampu mendeteksi virus melalui keberadaan RNA virus. Tujuan penelitian adalah mengetahui efektivitas metode RT-PCR dalam mendeteksi penyakit pada ikan nila (Oreochromis niloticus) di BKIPM Medan I. Metode penelitian menggunakan metode eksperimen bersifat kualitatif dilaksanakan di Laboratorium Biomolekuler di Balai Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan (BKIPM Medan I). Sampel ikan nila berasal dari tambak ikan nila di Kecamatan Porsea dan Laguboti, Toba Samosir, Sumatera Utara yang terdiri atas masing-masing 15 ekor ikan nila berumur 30 hari atau ikan nila yang sudah meng...
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method detect the virus through the presence of its RNA. The purpose of this study is to determine the effectiveness of RT-PCR method in detecting disease in tilapia (Oreochromis niloticus) at the Fish Quarantine Station for Quality Control and Safety of Fishery Products in Medan I (BKIPM Medan I). Qualitative research method was conducted in BKIPM Medan I.
Tilapia sample were taken from tilapia pond in Porsea and Laguboti, Toba Samosir, North Sumatera. Fifteen tilapias were taken from each pond. The sample criteria was 30 days old tilapia or tilapia that shown clinical symptom. RT-PCR method was perform at sample brain, kidney, and liver. The result from RT-PCR than analyzed and compared with the literature. The results of the study using the RT-PCR method on samples of tilapia (Oreochromis niloticus) from Porsea sub-district showed positive infected with TILV with the results of electrophoresis rna band TiLV appeared at 250 bp and the percentage of infestation of 66.67%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. So it can be oncluded that RT-PCR effectively detects TILV disease in tilapia fish.
method detect the virus through the presence of its RNA. The purpose of this study is to determine the effectiveness of RT-PCR method in detecting disease in tilapia (Oreochromis niloticus) at the Fish Quarantine Station for Quality Control and Safety of Fishery Products in Medan I (BKIPM Medan I). Qualitative research method was conducted in BKIPM Medan I.
Tilapia sample were taken from tilapia pond in Porsea and Laguboti, Toba Samosir, North Sumatera. Fifteen tilapias were taken from each pond. The sample criteria was 30 days old tilapia or tilapia that shown clinical symptom. RT-PCR method was perform at sample brain, kidney, and liver. The result from RT-PCR than analyzed and compared with the literature. The results of the study using the RT-PCR method on samples of tilapia (Oreochromis niloticus) from Porsea sub-district showed positive infected with TILV with the results of electrophoresis rna band TiLV appeared at 250 bp and the percentage of infestation of 66.67%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. While fish from Laguboti subdistrict there is no indication of TILV disease with the percentage of tilapia infestation (Oreochromis niloticus) affected by Tilapia Lake Virus (TiLV) disease by 0%. So it can be oncluded that RT-PCR effectively detects TILV disease in tilapia fish.