Papers by Mounia El KHADIR
Japanese journal of infectious diseases, Jun 28, 2024
Revue D Epidemiologie Et De Sante Publique, May 1, 2018
Diagnostic Microbiology and Infectious Disease, Jul 1, 2021
Helicobacter pylori infection is the most important etiological factor in gastroduodenal diseases... more Helicobacter pylori infection is the most important etiological factor in gastroduodenal diseases development. Its evolution is influenced by several factors, including bacterial virulence genes such as cagA and cagE. This work aimed to evaluate the predictive value of cagE alone and in combination with cagA and CagA-EPIYA-C motifs number as a marker of the infection evolution. A total of 823 H. pylori DNA extracted from biopsies of consenting patients suffering from gastritis, peptic ulcer, or gastric cancer. The cagE, cagA status and cagA 3' region polymorphism were determined by PCR. The analysis shows that the risk of duodenal ulcer is 1.97-fold higher (CI = 1.18-3.30) in patients infected by strains cagA+/cagE+. And the risk of gastric cancer is 5.19-fold higher (CI = 1.18-22.70) in patients harboring strains cagE+/2EPIYA-C. The results suggest that cagE in combination with cagA-EPIYA-C motifs number can be used as predictive biomarker of H. pylori infection evolution.
Journal of The Chinese Medical Association, Jul 1, 2016
Background: Finding a simple, accurate, and noninvasive diagnosis method is a substantial challen... more Background: Finding a simple, accurate, and noninvasive diagnosis method is a substantial challenge for the detection of Helicobacter pylori. The aim of the present study was to compare the presence of H. pylori urease antigen in saliva with the presence of this bacterium in gastric mucosa. Methods: Saliva samples and gastric biopsies were taken from 153 consenting Moroccan patients. Saliva samples were analyzed using an immunochromatographic test for urease antigen H. pylori detection. Thereafter, the gastric biopsies were analyzed by histology and polymerase chain reaction (PCR) to detect this bacterium. Results: From a total of 153 recruited Moroccan patients, H. pylori was detected in 28 (18.30%), 87 (57.24%), and 69 (45.10%) cases by saliva test, histology, and PCR, respectively. A significant association was observed between the presence of H. pylori antigen in saliva and age. However, no association was found with sex, H. pylori virulence factors, gastric disease outcome, and density of the bacterium on the gastric mucosa. Considering that only 90 patients presented concordant results on H. pylori diagnosis (positive or negative) by both histology and PCR, the immunochromatographic test showed very low sensitivity (29.79%) and high specificity (90.70%). Of these two tests, the positive and negative predictive values were 77.78% and 54.17%, respectively. The accuracy of the test for salivary detection of urease antigen H. pylori was 58.89%. Conclusion: This study demonstrated a low detection rate of H. pylori antigens in saliva compared with the presence of this bacterium in gastric mucosa, suggesting that saliva cannot be used as a suitable sample for the diagnosis of H. pylori in our study population.
Revue D Epidemiologie Et De Sante Publique, May 1, 2018
systématique au dossier médical garantissant qualité et validité des données. Le premier volet co... more systématique au dossier médical garantissant qualité et validité des données. Le premier volet concerne les patients traités pour cancer du sein métastatique (CSM). Cette cohorte représente 35 % des patients traités en France mais n'est pas représentative du niveau national. La cohorte Cancer de l'INCa est constituée de tous les patients atteints d'un cancer en France depuis 2010 ayant entraîné des soins à l'hôpital, en ville, ou la mise en affection de longue durée (ALD). Les données collectées sont issues du Système national des données de santé. Un travail a été amorcé entre l'INCa et Unicancer afin d'identifier des paramètres pour extrapoler au niveau national les résultats issus de la cohorte ESME CSM. Méthodes La première étape a consisté à reconstruire, à partir de la cohorte Cancer, une cohorte de patients ayant un CSM diagnostiqué en 2013 (maximum 36 mois après le primitif) et dont le premier traitement a été réalisé dans un Centre de lutte contre le cancer (CLCC). Les patients ont été identifiés via les codes diagnostics issus du PMSI et de l'ALD, et les traitements via le PMSI et les données de remboursement de ville. Résultats Sur l'année 2013, respectivement 1225 et 1319 patients avec un CSM ont été identifiés dans la cohorte ESME CSM et la cohorte Cancer. Les caractéristiques des patients et de leur traitement sont décrites. Discussion La population des patients ayant eu un CSM en 2013 (tumeur primitive en 2011, 2012 ou 2013) identifiée à partir de la cohorte Cancer est plus faible (−7,1 %). Les caractéristiques des patients et de leur maladie sont cohérentes entre les deux échantillons en tenant compte du décalage des effectifs liés à la différence entre les dates de référence utilisées par les deux cohortes (date de diagnostic clinique versus date de première prise en charge). Pour cette population spécifique, des variables liées au patient, aux métastases et à l'établissement de traitement permettront de définir des paramètres d'extrapolation des résultats issus de la cohorte ESME CSM. Conclusion Ces résultats encouragent à identifier des paramètres d'extrapolation des résultats de la cohorte ESME CSM. La même démarche sera adoptée sur les deux autres cohortes du programme ESME. Mots clés Cancer du sein métastatique ; Cohorte ; SNDS ; CLCC Déclaration de liens d'intérêts Les auteurs déclarent ne pas avoir de liens d'intérêts.
Infection, Genetics and Evolution, Dec 1, 2018
Background: The pathogenicity of cagA-positive H. pylori strains is associated with the number an... more Background: The pathogenicity of cagA-positive H. pylori strains is associated with the number and type of repeated sequences named EPIYA located in the C-terminal region of the CagA protein. The aim of this study is to determine the polymorphism of the H. pylori cagA 3' region circulating in Morocco and its association with different gastric pathologies. Methods: A total of 1353 consenting patients, were recruited in this study. The gastric biopsies performed during endoscopy were used for histological examination and for molecular characterization of H. pylori. The study of the type and number of "EPIYA" motif was identified by PCR directly on H. pylori positive biopsies. Results: Of all the biopsies, the infection rate was 61.1%. The cagA gene was amplified in 68.9% of the cases and the analysis of the 3' region of cagA showed the exclusive presence of the "Western CagA" type with a predominance of the EPIYA-ABC motif (71.4%). The number of EPIYA-C motif varies from 0 to 2. The multinomial analysis shows that the infection with strains of H. pylori having two EPIYA-C motifs is a factor that increases the risk of developing gastric cancer compared to gastritis cases with strains lacking this motif (OR=11.64; CI: 3.34-45.15), whereas this risk is 6 fold higher in comparison with duodenal ulcer cases (OR=6, CI: 1.29-27.76). Conclusions: The results of this study suggest that the number of EPIYA-C motifs might be useful as a predictive marker of the infection evolution and will help in the identification of patients at high risk of developing gastric cancer.
Revue D Epidemiologie Et De Sante Publique, May 1, 2018
Journal of Biosciences and Medicines, 2018
Functional dyspepsia constitutes by its impact in quality of life and socioeconomic impact areal ... more Functional dyspepsia constitutes by its impact in quality of life and socioeconomic impact areal public health problem (40% of the adult population in the Western world). The prevalence of infection with Helicobacter pylori (HP) in patients with functional dyspepsia is about 30%-70%. The aim of our study is to observe the improvement of functional dyspepsia (FD) after Helicobacter pylori (HP) eradication. Methods: This is a prospective study over a period of 68 months (May 2009-January 2015). We included all patients aged over 15 years, with functional dyspepsia defined by the Rome III criteria. The diagnosis of HP was made by histology and/or PCR. Patients infected with HP were treated in single-blind: standard triple therapy for 7 days or sequential treatment for 10 days. HP eradication control was made after: 3 months, 6 months and one year of treatment. Results: During the study period, 1190 patients were included of which 250 patients (21%) were in functional dyspepsia according to the Rome III criteria. The average age in patients with functional dyspepsia was 49 years [16-80], sex ratio M/F was 0.58 (N = 92/158). 60% of patients were older than 45 years (N = 150). Chronic smoking was found in 20% of patients (N = 50). One hundred and seventy five patients (70%) had HP infection (N = 175). One hundred and sixty five patients received the treatment of Helicobacter pylori (94.28%), 10 patients were lost to follow (5.71%). The eradication of HP was obtained in 98.41% with sequential treatment versus 88.
International Journal of Cancer, Jul 6, 2020
Helicobacter pylori infection induces inflammation of the gastric mucosa, which may progress to p... more Helicobacter pylori infection induces inflammation of the gastric mucosa, which may progress to precancerous lesions and gastric cancer. The gastric histo‐pathological damages may be associated with some virulence genes of the bacterium, notably vacA and cagA genes. To establish correlations between these genes and the lesions, biopsies from 1303 adults consenting patients that were previously analyzed by PCR to characterize vacA‐s vacA‐m, vacA‐i regions and cagA 3′ region polymorphism, were used. The highest average age was obtained in patients with intestinal metaplasia (53.65 ± 15.26 years) and gastric cancer (53.60 ± 14.32 years). Thus, these lesions are more frequent in elderly and male subjects. Tobacco smoking was significantly associated with neutrophilic activity (P = .02). No significant association was obtained between patients with chronic inflammation and vacA and cagA H. pylori genotypes. However, a significant association has been obtained between this lesion and cagA+ in aged patients (P = .02), while intestinal metaplasia was significantly associated with vacAi1 and vacAm1 separately (P < .01 and .01). Also, a significant association was obtained between intestinal metaplasia and strains with one EPIYA‐C motif in young patients (P = .001). Interestingly, a significant association was obtained between gastric cancer and cagA+, vacAi1, vacAm1 H. pylori genotypes and also with two EPIYA‐C motifs independently of age groups (all P < .05). The results of our study show that H. pylori vacAi1 could be more potent than the other H. pylori virulent factors for predicting the precancerous gastric lesions, confirming that this gene may be helpful to identify patients at high risk for gastric cancer.
<p>Distribution of <i>vacA</i> and <i>cagA</i> combination of <i... more <p>Distribution of <i>vacA</i> and <i>cagA</i> combination of <i>H</i>. <i>pylori</i> in duodenal ulcers and gastric cancer cases.</p
<p>Correlation between <i>vacA</i> mosaicism and <i>cagA</i> status... more <p>Correlation between <i>vacA</i> mosaicism and <i>cagA</i> status of <i>H</i>.<i>pylori</i> strains and their distribution according to the gender and to the age.</p
<p><i>H. pylori</i> status associated to risk factors.</p
<p>Association between <i>H</i>. <i>pylori vacA</i> alleles in stud... more <p>Association between <i>H</i>. <i>pylori vacA</i> alleles in studied isolates from Moroccan patients with single-strains infections.</p
<p>Distribution of Demographic and risk factors of the gastro duodenal diseases.</p
<p>Distribution of demographic and <i>vacA</i> alleles (single and associated) ... more <p>Distribution of demographic and <i>vacA</i> alleles (single and associated) of <i>H</i>. <i>pylori</i> in duodenal ulcers and gastric cancer cases.</p
<p><sup>*</sup><i>H.pylori</i> PCR result</p
<p>Risk for GC in relation to vacAs/cagA combination and i- and m-region of <i>vacA&l... more <p>Risk for GC in relation to vacAs/cagA combination and i- and m-region of <i>vacA</i> gene in a simple logistic regression analysis.</p
<p>Values were expressed % (n).</p
Diagnostic microbiology and infectious disease, 2021
Helicobacter pylori infection is the most important etiological factor in gastroduodenal diseases... more Helicobacter pylori infection is the most important etiological factor in gastroduodenal diseases development. Its evolution is influenced by several factors, including bacterial virulence genes such as cagA and cagE. This work aimed to evaluate the predictive value of cagE alone and in combination with cagA and CagA-EPIYA-C motifs number as a marker of the infection evolution. A total of 823 H. pylori DNA extracted from biopsies of consenting patients suffering from gastritis, peptic ulcer, or gastric cancer. The cagE, cagA status and cagA 3' region polymorphism were determined by PCR. The analysis shows that the risk of duodenal ulcer is 1.97-fold higher (CI = 1.18-3.30) in patients infected by strains cagA+/cagE+. And the risk of gastric cancer is 5.19-fold higher (CI = 1.18-22.70) in patients harboring strains cagE+/2EPIYA-C. The results suggest that cagE in combination with cagA-EPIYA-C motifs number can be used as predictive biomarker of H. pylori infection evolution.
International Journal of Cancer
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Papers by Mounia El KHADIR